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The map label for this gene is ksgA [H]

Identifier: 162446903

GI number: 162446903

Start: 19492

End: 20280

Strand: Direct

Name: ksgA [H]

Synonym: ACL_0016

Alternate gene names: 162446903

Gene position: 19492-20280 (Clockwise)

Preceding gene: 162446902

Following gene: 162446904

Centisome position: 1.3

GC content: 29.28

Gene sequence:

>789_bases
ATGCATCAAGCTAAAAAAAGATTTGGTCAAAACTTTTTAACAGATAAGAATTTATTGAAAAAGATTGTTGACTCAGCACA
AATTCATGATAAAAACGTATTAGAAATTGGACCAGGGTTAGGTGCACTAACTCAGTTTATAGTTAAAGATGCCAAAAAGT
ATCTAGCCTATGAAATTGACTACTCTCTAAAAGATGTTTTAGAAAATTTCTTAAGTGAAGATAGCAACATCATATTCCAA
GACTTTTTAGAATCTGATGTCACAAATGATTTAGATACATATTTTAAAGGTGAACCAATTCACTTGATTGGTAATTTACC
ATACTACATTACAACACCGATTATTTTTAAATTCTTAGAAATAGATCAACTTAAAACAGCAACAATCATGGTCCAAAAAG
AAGTGGGTGACCGTATGATTAGCAAGAAGAATTTAAAAAGCTATAATGCCTTTTCTGCGATTCTACAATACTTTACAGAT
GTAAGGTTGGTTGTTAATGTAAATAGAAAAATGTTTAACCCGGTACCTAAGGTAGATTCCATGGTTGTTCAACTAACTAA
AAAAGAAACTAAACTGGATAAATCATTAGAGCTTAAGTTTATTCAAATCGTTAAGTTGAGTTTTATGCATAAAAGAAAAA
CCTTGTTAAATAATCTATCTACAGGTCTAAACGAAGATAAAGCTAAGATACTAGAATCTTTAAAGTCGCTTAATTTAGAT
GAGAAGACAAGAGCTGAATCACTAAGTGTAGATGATTTTATTACACTTACTGAAAAACTTTATAAATAA

Upstream 100 bases:

>100_bases
TTAACCAGCAAATTAAACCTAACCTATAGTAACTCCAAGACACTGATACAAAGGCTTAATTGGCTAGGGTTAAGTTATAA
AGATTTAGAGGATATATTAT

Downstream 100 bases:

>100_bases
AATAACTAAAAATATTGATCGATTGTATCAATATTTTTTTATTTATATAAGGACAATCAAATAAAATACATAAATAATTG
CATAAAAACATATTAAGGAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MHQAKKRFGQNFLTDKNLLKKIVDSAQIHDKNVLEIGPGLGALTQFIVKDAKKYLAYEIDYSLKDVLENFLSEDSNIIFQ
DFLESDVTNDLDTYFKGEPIHLIGNLPYYITTPIIFKFLEIDQLKTATIMVQKEVGDRMISKKNLKSYNAFSAILQYFTD
VRLVVNVNRKMFNPVPKVDSMVVQLTKKETKLDKSLELKFIQIVKLSFMHKRKTLLNNLSTGLNEDKAKILESLKSLNLD
EKTRAESLSVDDFITLTEKLYK

Sequences:

>Translated_262_residues
MHQAKKRFGQNFLTDKNLLKKIVDSAQIHDKNVLEIGPGLGALTQFIVKDAKKYLAYEIDYSLKDVLENFLSEDSNIIFQ
DFLESDVTNDLDTYFKGEPIHLIGNLPYYITTPIIFKFLEIDQLKTATIMVQKEVGDRMISKKNLKSYNAFSAILQYFTD
VRLVVNVNRKMFNPVPKVDSMVVQLTKKETKLDKSLELKFIQIVKLSFMHKRKTLLNNLSTGLNEDKAKILESLKSLNLD
EKTRAESLSVDDFITLTEKLYK
>Mature_262_residues
MHQAKKRFGQNFLTDKNLLKKIVDSAQIHDKNVLEIGPGLGALTQFIVKDAKKYLAYEIDYSLKDVLENFLSEDSNIIFQ
DFLESDVTNDLDTYFKGEPIHLIGNLPYYITTPIIFKFLEIDQLKTATIMVQKEVGDRMISKKNLKSYNAFSAILQYFTD
VRLVVNVNRKMFNPVPKVDSMVVQLTKKETKLDKSLELKFIQIVKLSFMHKRKTLLNNLSTGLNEDKAKILESLKSLNLD
EKTRAESLSVDDFITLTEKLYK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=30.1418439716312, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI7657198, Length=278, Percent_Identity=28.4172661870504, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=32.5757575757576, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25141369, Length=284, Percent_Identity=27.4647887323944, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25146882, Length=278, Percent_Identity=29.4964028776978, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=215, Percent_Identity=28.3720930232558, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358017, Length=280, Percent_Identity=28.2142857142857, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21357273, Length=283, Percent_Identity=29.3286219081272, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30213; Mature: 30213

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQAKKRFGQNFLTDKNLLKKIVDSAQIHDKNVLEIGPGLGALTQFIVKDAKKYLAYEID
CCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC
YSLKDVLENFLSEDSNIIFQDFLESDVTNDLDTYFKGEPIHLIGNLPYYITTPIIFKFLE
CHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
IDQLKTATIMVQKEVGDRMISKKNLKSYNAFSAILQYFTDVRLVVNVNRKMFNPVPKVDS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCHHHH
MVVQLTKKETKLDKSLELKFIQIVKLSFMHKRKTLLNNLSTGLNEDKAKILESLKSLNLD
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
EKTRAESLSVDDFITLTEKLYK
HHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHQAKKRFGQNFLTDKNLLKKIVDSAQIHDKNVLEIGPGLGALTQFIVKDAKKYLAYEID
CCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC
YSLKDVLENFLSEDSNIIFQDFLESDVTNDLDTYFKGEPIHLIGNLPYYITTPIIFKFLE
CHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
IDQLKTATIMVQKEVGDRMISKKNLKSYNAFSAILQYFTDVRLVVNVNRKMFNPVPKVDS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCHHHH
MVVQLTKKETKLDKSLELKFIQIVKLSFMHKRKTLLNNLSTGLNEDKAKILESLKSLNLD
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
EKTRAESLSVDDFITLTEKLYK
HHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA