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The map label for this gene is recF [H]

Identifier: 162446892

GI number: 162446892

Start: 4690

End: 5739

Strand: Direct

Name: recF [H]

Synonym: ACL_0005

Alternate gene names: 162446892

Gene position: 4690-5739 (Clockwise)

Preceding gene: 162446891

Following gene: 162446893

Centisome position: 0.31

GC content: 28.76

Gene sequence:

>1050_bases
ATGATTACTTCAATTGAATTAAGAAACTTTAGAAATTTAGAAAATTATAAAGTTTTAATCAATAAACCACTTGTGATTAT
TCAAGGCTTAAACGGAGTTGGAAAGACTTCCATTCTAGAATCGATTTATTTTGCTGCAACAACAAAGTCACATCGATCAA
GCGTTGAAAAGGATATGATTCAATATGATAAACCCTATGCCTCAGTAAAACTTATAGAGGATTCAAAACTACATGAGATT
GTACTTACACCAAATGGTAAAAGAACAACCATCAATAAATCAGAAGTTAGAAAAATTTCTGACTATATAGGTCAATTAAG
AGTTGTAATGTTTGCTCCTGAAGACTTAATGCTTATCAAGGGAAGTCCTTCCGAGCGACGTTACTTTTTAGATATGGAAC
TCATGCAAGTTTCTAAAACCTATTTAAGAAATTTAAATAGTTATAAAAAGATCTTAAAACAAAGAAATGCTTTACTTAAG
AAAAACAGAAATTTAACAGATTATACATTCTTAAATATTTTAGGTGAACAACTCTATGATGTAGGTATTCAAATTTTTGA
TGAAAGACAAAAGTTTATTGAAGCACTTAATCAAAAATTTAAAACAATACAAACAAAGTATAAGGACTTTGAAGTTGAGA
TGCTATATGAACCTAACGTCACTAAAGAAAATTTCCTTAAACATCTTAAAACAAAACAAAAACAAGATATTATGTATGAG
ACAACAACTGCCGGTATACATAAAGACGACTTTAAACTACTTTATAAGGGTTTGAATGCAAAAGACAGTGCATCACAGGG
AACAAGTAGATTAATTGTCATAGAACTCAAATTAGCCTTGTTAGAATGGATTAAAGAAGTAACTAAGACAGATGCAATCT
TACTATTAGATGATGTATTATCTGAATTAGATTTAGAACGTCAAAACTTGTTTATGAGTCAACTATCTAAAAATCATCAA
GTATTTATTACAACAGCACTACCTATAAATGGACACATAGATTTTCAAAAAATAGTATTACAAGAAGGAGAAACAATCAA
TGCCAAATAA

Upstream 100 bases:

>100_bases
AGCATAAAGTATTACTAAATGATGCAGAAGTTTACGAGAAGAAAAAGAAGATTAAAAAAGGTGATGTCGTTACAATTAAC
GGCAACAAGATTTTAATTAA

Downstream 100 bases:

>100_bases
TAATAACAGTCATTATACTGCAGATAATATTCAAATATTGGAAGGATTAGAAGCAGTTAAAAAAAGACCAGGTATGTATA
TCGGATCCACAGGTGAACGT

Product: DNA replication and repair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MITSIELRNFRNLENYKVLINKPLVIIQGLNGVGKTSILESIYFAATTKSHRSSVEKDMIQYDKPYASVKLIEDSKLHEI
VLTPNGKRTTINKSEVRKISDYIGQLRVVMFAPEDLMLIKGSPSERRYFLDMELMQVSKTYLRNLNSYKKILKQRNALLK
KNRNLTDYTFLNILGEQLYDVGIQIFDERQKFIEALNQKFKTIQTKYKDFEVEMLYEPNVTKENFLKHLKTKQKQDIMYE
TTTAGIHKDDFKLLYKGLNAKDSASQGTSRLIVIELKLALLEWIKEVTKTDAILLLDDVLSELDLERQNLFMSQLSKNHQ
VFITTALPINGHIDFQKIVLQEGETINAK

Sequences:

>Translated_349_residues
MITSIELRNFRNLENYKVLINKPLVIIQGLNGVGKTSILESIYFAATTKSHRSSVEKDMIQYDKPYASVKLIEDSKLHEI
VLTPNGKRTTINKSEVRKISDYIGQLRVVMFAPEDLMLIKGSPSERRYFLDMELMQVSKTYLRNLNSYKKILKQRNALLK
KNRNLTDYTFLNILGEQLYDVGIQIFDERQKFIEALNQKFKTIQTKYKDFEVEMLYEPNVTKENFLKHLKTKQKQDIMYE
TTTAGIHKDDFKLLYKGLNAKDSASQGTSRLIVIELKLALLEWIKEVTKTDAILLLDDVLSELDLERQNLFMSQLSKNHQ
VFITTALPINGHIDFQKIVLQEGETINAK
>Mature_349_residues
MITSIELRNFRNLENYKVLINKPLVIIQGLNGVGKTSILESIYFAATTKSHRSSVEKDMIQYDKPYASVKLIEDSKLHEI
VLTPNGKRTTINKSEVRKISDYIGQLRVVMFAPEDLMLIKGSPSERRYFLDMELMQVSKTYLRNLNSYKKILKQRNALLK
KNRNLTDYTFLNILGEQLYDVGIQIFDERQKFIEALNQKFKTIQTKYKDFEVEMLYEPNVTKENFLKHLKTKQKQDIMYE
TTTAGIHKDDFKLLYKGLNAKDSASQGTSRLIVIELKLALLEWIKEVTKTDAILLLDDVLSELDLERQNLFMSQLSKNHQ
VFITTALPINGHIDFQKIVLQEGETINAK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:
GO:0000166: DNA replication and repair protein recF
GO:0003677: DNA replication and repair protein recF
GO:0003697: DNA replication and repair protein recF
GO:0005524: DNA replication and repair protein recF
GO:0005694: DNA replication and repair protein recF
GO:0005737: DNA replication and repair protein recF
GO:0006260: DNA replication and repair protein recF
GO:0006281: DNA replication and repair protein recF
GO:0006974: DNA replication and repair protein recF
GO:0009432: DNA replication and repair protein recF

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=332, Percent_Identity=26.2048192771084, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40525; Mature: 40525

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSIELRNFRNLENYKVLINKPLVIIQGLNGVGKTSILESIYFAATTKSHRSSVEKDMI
CCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
QYDKPYASVKLIEDSKLHEIVLTPNGKRTTINKSEVRKISDYIGQLRVVMFAPEDLMLIK
HHCCCCCEEEEEECCCEEEEEECCCCCEECCCHHHHHHHHHHHCCEEEEEECCCCEEEEE
GSPSERRYFLDMELMQVSKTYLRNLNSYKKILKQRNALLKKNRNLTDYTFLNILGEQLYD
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VGIQIFDERQKFIEALNQKFKTIQTKYKDFEVEMLYEPNVTKENFLKHLKTKQKQDIMYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCEEE
TTTAGIHKDDFKLLYKGLNAKDSASQGTSRLIVIELKLALLEWIKEVTKTDAILLLDDVL
ECCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEHHHHHH
SELDLERQNLFMSQLSKNHQVFITTALPINGHIDFQKIVLQEGETINAK
HHHCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MITSIELRNFRNLENYKVLINKPLVIIQGLNGVGKTSILESIYFAATTKSHRSSVEKDMI
CCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
QYDKPYASVKLIEDSKLHEIVLTPNGKRTTINKSEVRKISDYIGQLRVVMFAPEDLMLIK
HHCCCCCEEEEEECCCEEEEEECCCCCEECCCHHHHHHHHHHHCCEEEEEECCCCEEEEE
GSPSERRYFLDMELMQVSKTYLRNLNSYKKILKQRNALLKKNRNLTDYTFLNILGEQLYD
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VGIQIFDERQKFIEALNQKFKTIQTKYKDFEVEMLYEPNVTKENFLKHLKTKQKQDIMYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCEEE
TTTAGIHKDDFKLLYKGLNAKDSASQGTSRLIVIELKLALLEWIKEVTKTDAILLLDDVL
ECCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEHHHHHH
SELDLERQNLFMSQLSKNHQVFITTALPINGHIDFQKIVLQEGETINAK
HHHCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10779957 [H]