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The map label for this gene is gyrB [H]

Identifier: 162446893

GI number: 162446893

Start: 5729

End: 7636

Strand: Direct

Name: gyrB [H]

Synonym: ACL_0006

Alternate gene names: 162446893

Gene position: 5729-7636 (Clockwise)

Preceding gene: 162446892

Following gene: 162446894

Centisome position: 0.38

GC content: 34.8

Gene sequence:

>1908_bases
ATGCCAAATAATAATAACAGTCATTATACTGCAGATAATATTCAAATATTGGAAGGATTAGAAGCAGTTAAAAAAAGACC
AGGTATGTATATCGGATCCACAGGTGAACGTGGACTTCACCACTTAGTTTGGGAAATCGTTGATAACTCAATTGACGAGG
CTTTAGGCGGATATGCAGATGAAATCACCATAGAGATTTTAAAAGGTGAAGTGATACGCGTTACTGATAATGGTCGTGGT
ATTCCAGTAGATATCCACCCTAAGACAAAAAGACCAGCTGTTGAAACGATTTTAACAACGCTTCATGCAGGTGGTAAGTT
TGATAAAGGTTCTTATAAAGTTTCTGGTGGTCTACACGGTGTAGGGGCATCTGTAGTCAACGGTTTATCTGAATGGTTTG
TTGTAGAAATTCATAAAGATGGAACGATTTATGAACAAAAATATGAACGTGGTATTCCAGCATATGACCTTAAAGTAAAA
GGAAGTACAGATAAATCAGGAACGATTATTTCATTCCAAGCGGATCCACTTATTTTTACTGAAACAGTTATTTATAACTA
TGAAACATTAAGAACAAGAATCCAACAATTAGCATTCTTAAATAAAGGACTTAAACTGAATTTAATCGATGATCGTTTTG
AAGAAAACAAGTCTGAATCATTCCACTACGAAGGTGGTATTACAGAATATGTTAAGTTCTTAAATCAGTCTAAATCAAAG
ATTCATAATGACATTATTTATATCGATAAAGAACAAGATGGCATCACGGTAGAACTTGCTATGCAGTTTGTAGATAGCTA
TTCTCCAAACCTACATTCATTTACAAATAACATCTCAACTACTGAAGGTGGAATGCACGAAGATGGCTTCAAGATGGCAT
TAACGCGTGTTATATCTAAGTATGCAGATGATTTAAAAATGAAAAAAGATGACAGTATTTCAGGTGAGGATACTAGAGAA
GGTTTAACTGCAATTATTTCAGTTAAACACCCTGAACCTCAATTTGAAGGACAAACAAAAACTAAATTAGGTAACCCTGA
AGTTCGTGCAATTACATCCCAAATTACTAGTGAAGCCATAGAACGTTTTTTAATGGAAAATCCAGCTCAAGCAAAAGCGA
TTGTTGAAAAAGTTTTACTTGCAACACGCGCACGTGTTGCTGCAAGAAAAGCAAAAGATTTAACAAGAAGAAAATCACCA
CTTGATGCTTTAGGATTTGCAAGTAAACTAGCGGATTGCCGCAGTAAAGATCCTGAAAAATCAGAAATCTATATTGTGGA
AGGGGACTCTGCGGGTGGTTCTGCAAAACAAGGTAGAGACTCAGAATTCCAAGCAATTATGCCATTACGTGGTAAGGTCT
TAAACGTAGAAAAAGCACGTCTAGATAAAATGCTTTCTAATAAAGAAATTGTGAACTTAATCCAAGCTATGGGTACAGGT
ATCTCTGATGAGTTTGATATTACCAAAGCACGTTATCATAAAATTGTTATTATGACCGATGCTGACGTCGACGGTGCACA
TATTAGAACCCTACTATTAACATTCTTATTTAGACATATGAGACCATTAATTGATGCTGGTTATGTATATGCAGCTCAAC
CACCACTATATAAAATTTCTTGGGGCAGAAATTTTCAATATGCTTATAATGAACAAGAATTAAATGAACTACTAAAAACA
ATAGAAACAAAACCATCTATTCAACGTTATAAAGGTTTAGGAGAAATGGATGCTGAGCAATTATGGGATACTACAATGGA
TCCTGCAAAGCGTACTTTACTTCAAATTAAATTAGAAGATGCTATCGAAGCCGATCAAGTGTTTTCTATGTTAATGGGTG
AAGAAGTTGAACCAAGAAAAGAATTTATTCAAAATAATGCACAATATGCAACAGATATTGACGCATAG

Upstream 100 bases:

>100_bases
CAACTATCTAAAAATCATCAAGTATTTATTACAACAGCACTACCTATAAATGGACACATAGATTTTCAAAAAATAGTATT
ACAAGAAGGAGAAACAATCA

Downstream 100 bases:

>100_bases
GAAAGGATGTAAATAATAATGAGTGAAAATACAGATAAACTACATGATAAGATTAAAGAAGTAAACATTACTTCAGAGAT
GAAAACATCCTTCTTAAACT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MPNNNNSHYTADNIQILEGLEAVKKRPGMYIGSTGERGLHHLVWEIVDNSIDEALGGYADEITIEILKGEVIRVTDNGRG
IPVDIHPKTKRPAVETILTTLHAGGKFDKGSYKVSGGLHGVGASVVNGLSEWFVVEIHKDGTIYEQKYERGIPAYDLKVK
GSTDKSGTIISFQADPLIFTETVIYNYETLRTRIQQLAFLNKGLKLNLIDDRFEENKSESFHYEGGITEYVKFLNQSKSK
IHNDIIYIDKEQDGITVELAMQFVDSYSPNLHSFTNNISTTEGGMHEDGFKMALTRVISKYADDLKMKKDDSISGEDTRE
GLTAIISVKHPEPQFEGQTKTKLGNPEVRAITSQITSEAIERFLMENPAQAKAIVEKVLLATRARVAARKAKDLTRRKSP
LDALGFASKLADCRSKDPEKSEIYIVEGDSAGGSAKQGRDSEFQAIMPLRGKVLNVEKARLDKMLSNKEIVNLIQAMGTG
ISDEFDITKARYHKIVIMTDADVDGAHIRTLLLTFLFRHMRPLIDAGYVYAAQPPLYKISWGRNFQYAYNEQELNELLKT
IETKPSIQRYKGLGEMDAEQLWDTTMDPAKRTLLQIKLEDAIEADQVFSMLMGEEVEPRKEFIQNNAQYATDIDA

Sequences:

>Translated_635_residues
MPNNNNSHYTADNIQILEGLEAVKKRPGMYIGSTGERGLHHLVWEIVDNSIDEALGGYADEITIEILKGEVIRVTDNGRG
IPVDIHPKTKRPAVETILTTLHAGGKFDKGSYKVSGGLHGVGASVVNGLSEWFVVEIHKDGTIYEQKYERGIPAYDLKVK
GSTDKSGTIISFQADPLIFTETVIYNYETLRTRIQQLAFLNKGLKLNLIDDRFEENKSESFHYEGGITEYVKFLNQSKSK
IHNDIIYIDKEQDGITVELAMQFVDSYSPNLHSFTNNISTTEGGMHEDGFKMALTRVISKYADDLKMKKDDSISGEDTRE
GLTAIISVKHPEPQFEGQTKTKLGNPEVRAITSQITSEAIERFLMENPAQAKAIVEKVLLATRARVAARKAKDLTRRKSP
LDALGFASKLADCRSKDPEKSEIYIVEGDSAGGSAKQGRDSEFQAIMPLRGKVLNVEKARLDKMLSNKEIVNLIQAMGTG
ISDEFDITKARYHKIVIMTDADVDGAHIRTLLLTFLFRHMRPLIDAGYVYAAQPPLYKISWGRNFQYAYNEQELNELLKT
IETKPSIQRYKGLGEMDAEQLWDTTMDPAKRTLLQIKLEDAIEADQVFSMLMGEEVEPRKEFIQNNAQYATDIDA
>Mature_634_residues
PNNNNSHYTADNIQILEGLEAVKKRPGMYIGSTGERGLHHLVWEIVDNSIDEALGGYADEITIEILKGEVIRVTDNGRGI
PVDIHPKTKRPAVETILTTLHAGGKFDKGSYKVSGGLHGVGASVVNGLSEWFVVEIHKDGTIYEQKYERGIPAYDLKVKG
STDKSGTIISFQADPLIFTETVIYNYETLRTRIQQLAFLNKGLKLNLIDDRFEENKSESFHYEGGITEYVKFLNQSKSKI
HNDIIYIDKEQDGITVELAMQFVDSYSPNLHSFTNNISTTEGGMHEDGFKMALTRVISKYADDLKMKKDDSISGEDTREG
LTAIISVKHPEPQFEGQTKTKLGNPEVRAITSQITSEAIERFLMENPAQAKAIVEKVLLATRARVAARKAKDLTRRKSPL
DALGFASKLADCRSKDPEKSEIYIVEGDSAGGSAKQGRDSEFQAIMPLRGKVLNVEKARLDKMLSNKEIVNLIQAMGTGI
SDEFDITKARYHKIVIMTDADVDGAHIRTLLLTFLFRHMRPLIDAGYVYAAQPPLYKISWGRNFQYAYNEQELNELLKTI
ETKPSIQRYKGLGEMDAEQLWDTTMDPAKRTLLQIKLEDAIEADQVFSMLMGEEVEPRKEFIQNNAQYATDIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:
GO:0000166: DNA gyrase subunit B
GO:0003677: DNA gyrase subunit B
GO:0003916: DNA gyrase subunit B
GO:0003918: DNA gyrase subunit B
GO:0005524: DNA gyrase subunit B
GO:0005694: DNA gyrase subunit B
GO:0006265: DNA gyrase subunit B
GO:0016853: DNA gyrase subunit B

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=665, Percent_Identity=25.7142857142857, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI19913406, Length=652, Percent_Identity=24.5398773006135, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=50.8108108108108, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=36.8670886075949, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535065, Length=587, Percent_Identity=24.7018739352641, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=607, Percent_Identity=25.2059308072488, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI212645657, Length=196, Percent_Identity=26.0204081632653, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=590, Percent_Identity=28.3050847457627, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136538, Length=691, Percent_Identity=24.3125904486252, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71216; Mature: 71085

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNNNSHYTADNIQILEGLEAVKKRPGMYIGSTGERGLHHLVWEIVDNSIDEALGGYAD
CCCCCCCCEECCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCH
EITIEILKGEVIRVTDNGRGIPVDIHPKTKRPAVETILTTLHAGGKFDKGSYKVSGGLHG
HEEEEEECCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNGLSEWFVVEIHKDGTIYEQKYERGIPAYDLKVKGSTDKSGTIISFQADPLIFT
CHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCEEEE
ETVIYNYETLRTRIQQLAFLNKGLKLNLIDDRFEENKSESFHYEGGITEYVKFLNQSKSK
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCEEECCCHHHHHHHHHHHHHH
IHNDIIYIDKEQDGITVELAMQFVDSYSPNLHSFTNNISTTEGGMHEDGFKMALTRVISK
HCCCEEEEECCCCCEEHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
YADDLKMKKDDSISGEDTREGLTAIISVKHPEPQFEGQTKTKLGNPEVRAITSQITSEAI
HHHHHCCCCCCCCCCCCHHCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ERFLMENPAQAKAIVEKVLLATRARVAARKAKDLTRRKSPLDALGFASKLADCRSKDPEK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
SEIYIVEGDSAGGSAKQGRDSEFQAIMPLRGKVLNVEKARLDKMLSNKEIVNLIQAMGTG
CEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHCCC
ISDEFDITKARYHKIVIMTDADVDGAHIRTLLLTFLFRHMRPLIDAGYVYAAQPPLYKIS
CCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEE
WGRNFQYAYNEQELNELLKTIETKPSIQRYKGLGEMDAEQLWDTTMDPAKRTLLQIKLED
CCCCCCEECCHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHCCCCCHHHHHEEEEEHHH
AIEADQVFSMLMGEEVEPRKEFIQNNAQYATDIDA
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PNNNNSHYTADNIQILEGLEAVKKRPGMYIGSTGERGLHHLVWEIVDNSIDEALGGYAD
CCCCCCCEECCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCH
EITIEILKGEVIRVTDNGRGIPVDIHPKTKRPAVETILTTLHAGGKFDKGSYKVSGGLHG
HEEEEEECCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNGLSEWFVVEIHKDGTIYEQKYERGIPAYDLKVKGSTDKSGTIISFQADPLIFT
CHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCEEEE
ETVIYNYETLRTRIQQLAFLNKGLKLNLIDDRFEENKSESFHYEGGITEYVKFLNQSKSK
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCEEECCCHHHHHHHHHHHHHH
IHNDIIYIDKEQDGITVELAMQFVDSYSPNLHSFTNNISTTEGGMHEDGFKMALTRVISK
HCCCEEEEECCCCCEEHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
YADDLKMKKDDSISGEDTREGLTAIISVKHPEPQFEGQTKTKLGNPEVRAITSQITSEAI
HHHHHCCCCCCCCCCCCHHCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ERFLMENPAQAKAIVEKVLLATRARVAARKAKDLTRRKSPLDALGFASKLADCRSKDPEK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
SEIYIVEGDSAGGSAKQGRDSEFQAIMPLRGKVLNVEKARLDKMLSNKEIVNLIQAMGTG
CEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHCCC
ISDEFDITKARYHKIVIMTDADVDGAHIRTLLLTFLFRHMRPLIDAGYVYAAQPPLYKIS
CCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEE
WGRNFQYAYNEQELNELLKTIETKPSIQRYKGLGEMDAEQLWDTTMDPAKRTLLQIKLED
CCCCCCEECCHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHCCCCCHHHHHEEEEEHHH
AIEADQVFSMLMGEEVEPRKEFIQNNAQYATDIDA
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]