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The map label for this gene is pcnB [H]

Identifier: 162420585

GI number: 162420585

Start: 1032570

End: 1033892

Strand: Direct

Name: pcnB [H]

Synonym: YpAngola_A1008

Alternate gene names: 162420585

Gene position: 1032570-1033892 (Clockwise)

Preceding gene: 162419973

Following gene: 162421637

Centisome position: 22.92

GC content: 51.7

Gene sequence:

>1323_bases
ATGGCGATTATTCCGCGGGATCAGCACAATATCTCCCGCAGAGATATCAGTGACAATGCACTGAAGGTTCTCTATCGCCT
GAATAAATCAGGTTACGAAGCTTATCTGGTGGGCGGCGGTGTCCGCGACCTGCTGCTGGGCAGAAAACCCAAAGATTTCG
ATATCACCACCAGCGCCACCCCTGAACAGGTGCGAAAACTGTTCCGTAACTGTCGTTTGGTTGGCCGCCGCTTCCGTTTG
GCTCATGTGATGTTTGGCCCTGAAATTATTGAAGTAGCGACTTTCCGTGGCCACCACGAGCAGCAACAAGCAGAAGACAG
CGATAAGAACTCCTCCCAGCAGGCGCAAAATGGCATGTTGCTGCGTGACAACATCTTTGGCTCCATCGAAGATGATGCTC
AACGCCGTGATTTCACCATTAACAGCCTCTACTACGGTATTTCTGATTTTGCGCTGCGTGACTACACCGGCGGCTTACGT
GATCTGAAAGAAGGTATTATTCGTCTAATTGGCGACCCAGAAACCCGTTACCGTGAAGATCCGGTACGGATGCTGCGTGC
GGTTCGTTTTGCCGCAAAACTGGATATGTCAATCAGCCCGGAAACGGCAGAGCCTATACCGCGTCTGGCCTCACTGTTGC
GCGAAATCCCGCCCGCCCGCCTGTTTGAGGAGTCACTCAAGCTGCTGCAATCCGGCTACGGCTATAAAACCTATTTAAAA
CTGTGCGAATACCAGCTTTTCCAGCCGCTATTCCCACTGATTGCCCGTAATTTTACTGAACAGCATGATTCGCCAATGGA
GCGTATTCTGGTTCAGGTACTGAAAAATACCGACCACCGTCTGCATAATGATCAGCGGGTTAATCCTGCCTTCTTATTTG
CGGCGATGCTGTGGTATCCGCTGATTGAACATGCGCAGAAGTTAACGCAGGAAAGCGGCTTGGCCTATTACGATGCATTT
GCATTGGCCATGAATGATGTACTGGACGAAGAGTGTCGCTCACTGGCGATCCCTAAACGTATCACCTCACTGGTACGGGA
TATCTGGTTGTTACAACTGCGTCTGTCACGTCGTCAGGGGAAACGTGCCCATAAGCTGATGGAACACCCTAAATTCCGGG
CGGCTTACGATCTGTTACTCCTACGGGCAGAAGTGGAAAAAAATCACGAGCTGCAACGTTTGGCGCAGTGGTGGGGCGAA
TTCCAGGAAGCCACTCCCACGCAACAGAAATCCATGCTGAATACATTAGGTGCTGATCCTGCGCCACGGCGTTCACGTCC
ACGCCGCCCACGCAAGCCAGTACCCCGTAAAGAAGGGGTATAA

Upstream 100 bases:

>100_bases
AGTGCCCGGTGAAGATAACGTGGCCCGCAAAGAGAGAAGGCCCGCCAGAGCACATACTGGCCGCAAGGGTCACGCGGTGT
CGTCTTCGGAACAGCGCCAA

Downstream 100 bases:

>100_bases
TTTTACCATGATCCGGGTCTATATCGCGCTGGGTAGCAATTTGGCCATGCCACTACAACAGGTTAGCGCGGCACGGGAAG
CCTTGGCGCATCTGCCGCGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MAIIPRDQHNISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRL
AHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLR
DLKEGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLK
LCEYQLFQPLFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAF
ALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGE
FQEATPTQQKSMLNTLGADPAPRRSRPRRPRKPVPRKEGV

Sequences:

>Translated_440_residues
MAIIPRDQHNISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRL
AHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLR
DLKEGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLK
LCEYQLFQPLFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAF
ALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGE
FQEATPTQQKSMLNTLGADPAPRRSRPRRPRKPVPRKEGV
>Mature_439_residues
AIIPRDQHNISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLA
HVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRD
LKEGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKL
CEYQLFQPLFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFA
LAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEF
QEATPTQQKSMLNTLGADPAPRRSRPRRPRKPVPRKEGV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081691, Length=416, Percent_Identity=80.0480769230769, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=230, Percent_Identity=30.8695652173913, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=191, Percent_Identity=28.2722513089005, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281360112, Length=240, Percent_Identity=30, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357337, Length=240, Percent_Identity=30, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 51075; Mature: 50944

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIPRDQHNISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSAT
CCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCC
PEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGML
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCEE
LRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYRED
EECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCC
PVRMLRAVRFAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
LCEYQLFQPLFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYP
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
LIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADP
HHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
APRRSRPRRPRKPVPRKEGV
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AIIPRDQHNISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSAT
CCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCC
PEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGML
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCEE
LRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYRED
EECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCC
PVRMLRAVRFAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
LCEYQLFQPLFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYP
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
LIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADP
HHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
APRRSRPRRPRKPVPRKEGV
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]