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The map label for this gene is ftsI [H]

Identifier: 162148955

GI number: 162148955

Start: 3270358

End: 3272406

Strand: Direct

Name: ftsI [H]

Synonym: GDI_3185

Alternate gene names: 162148955

Gene position: 3270358-3272406 (Clockwise)

Preceding gene: 162148954

Following gene: 162148956

Centisome position: 82.92

GC content: 68.57

Gene sequence:

>2049_bases
ATGACCAACGGCCCGACCGACACACCAGACAGCATGCGGAGCGGGCCCTCCCGCCCCCAGCGGATGGATGTACGGATCAC
GGGCGACGACCTGCGCAGCCGGACGGCGCGCGAACGCACCCAGTCCCGCCTGCTGGGCGTCTCGGTCGGGTTCTGCGCCC
TGTTCGGCGCGGTGGCGCTGAAACTGGCCGCCGCCACCGTCATCATGCCGATGGAACCGGAACGCCGGCAGGTCGCCCCC
CAGGTGCCCACGATCCCCAAGAGCGATCCCAAGGGCATGATGGCCGGCGATTTCGCCCTGCCGCAGGTCCATCGCGCCTC
GATCACCGACCGCAACGGGCAGGTGCTGGCGATTTCGCTGCCCGTCGCGCAGGTCTATGCCAATCCGCAGGAAATGATCG
ACCCGCAGGACGCCGCGCAGAAGCTGAAGAGCGTGCTGCCCGCGCTGGATACGGACGAGACGATCCGCCGCCTGTCCACG
AAGAAGCAGTTCGTCTATCTGGCGCGCGATATCTCGCCGATGCAGGAAGTCGCGATCAACAACCTGGGCATTCCGGGCAT
CTATTTCGAACCGGGCGAGCGGCGCCATTACCCGCTGGGCCGCGTCGCCGCCCACATCATGGGCGCGGTCGACATCGACG
ATCACGGCGTGGCCGGCGTCGAACGCTTCCTGGACAAGCGGCTGGATTCCGACCGCACGCCCCTGCGCCTGTCGCTGGAC
GTGCGCATCCAGGCGGTGGTGCGCGACGAACTGGCGGCGGCCAAGGACATGTTCCAGGCCATCGGCGCGTGCGCCATCGT
GATGGACGTCAATACCGGCGAGATCATCGCCATGGTCAGCCTGCCCGATTACGATGCCAACGATTTCGGCCGCGCGGACC
CCGATGCCCGCTTCAACCGCGCCGTGACGGGCATGTACGAACCGGGCTCGACCTTCAAGCTGCAGACGGCCGCCATGGCG
CTGCAAATGGGTGTGGCGCATATCTGGGACCGGTTTTCCACCATTCCGATCCATATCGGCCGCTTTACCATTTCGGACAT
GAAGACGGACCATTTCGCCCCCTGGCTGTCGCTGCCCGGGGTCATGGCGTTCTCCTCCAACCCCGCCGCCGCCCATATCG
CGCTGGATGTCGGTGCCCAGCGCCAGCAGGACTGGCTGCGGAAGATGGGCTTCTTCTCCCCGGCGCCGATCGAACTGCCG
GAAGCCGGACGTCCCATCGTGCCCCAGACGCGCAACTGGGGGATTTCCACTGTCATGACGGTGGGCTTCGGCCATGGCGT
CGCCGAACCGCCGCTGGCCATCGTCCGGGGCACCGCCGCCACCGTGAATGGCGGGCTGCTTCTGCACCCCACCCTGCTGG
CCCGCGACGATGCCGGCACCGACGCGCCCGCCGCCCCGTCGTCCGACCCGTCGCCCACCGAACCCGGAGCGCCCGCCGCC
GCCGGCACGCGCGTCATCTCGCCCGAAATCTCGGCACTGCTGCGCCGCATCCTGCGCCTGGACGTGACACAGGGGACCGG
AAAATCGGCGGAATCACCGGGCTATTTCGTCGGCGGCAAGACAGGCACCGCCGAGAAGATCGGGCCGCATGGCGGCTATC
TGAAACATGTCAACGTCGCGGCCTTCACCAGCATCTTTCCGATGAACGCACCGCGCTACGCCGTCTACGTCATGCTCGAC
AGTCCCAAGGCGACGCCGCAGACCCATGGCTGGACCACGGCGGCGTGGAACGCCGCCCCCACGGTGTCCAAGATCGTGAC
CCGCATCGGCCCGATGCTGGGGCTGTTCCCCAACACCGCCAACGCCCAGGCCATCGACGAGGCGCTGGCCATCCCGATGG
AGCCGGCGGTGCCGCGCGGCTATCGCGCGCTGGGCCCGGGCAACGACCCCGGCGATCCCCGCAACGCACCGAAGCCGCCG
AAGACGCCGAAGAAGGGCGAGCGGCATGGCGACATGCCGGTGGCCGACCTGTCCACGCAGCCGCGCCTGCACGCCGCCCG
GGCAGAACGGGCCGCGCGGTCCTCCGACCCGATGGAATCCCGCGGATGA

Upstream 100 bases:

>100_bases
CAGGGGGTCCTCGCTGGGTTTCACGCATTCCGCTTCTCTTCCGCCCCCCGTCCCCGTCAGCAACTGACGACCCAGCCCCC
GATGACCTAGCGCAGGTACC

Downstream 100 bases:

>100_bases
CCCACCCCCTGTCCCAGGTGCTGGCGGCGGCCGGCCTGTCCGCCCCGCCCGGCGCCGACCCGGCGATTACCGGCGTGACC
GCCGACAGCCGGCGGGTGAC

Product: peptidoglycan synthetase ftsI

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MTNGPTDTPDSMRSGPSRPQRMDVRITGDDLRSRTARERTQSRLLGVSVGFCALFGAVALKLAAATVIMPMEPERRQVAP
QVPTIPKSDPKGMMAGDFALPQVHRASITDRNGQVLAISLPVAQVYANPQEMIDPQDAAQKLKSVLPALDTDETIRRLST
KKQFVYLARDISPMQEVAINNLGIPGIYFEPGERRHYPLGRVAAHIMGAVDIDDHGVAGVERFLDKRLDSDRTPLRLSLD
VRIQAVVRDELAAAKDMFQAIGACAIVMDVNTGEIIAMVSLPDYDANDFGRADPDARFNRAVTGMYEPGSTFKLQTAAMA
LQMGVAHIWDRFSTIPIHIGRFTISDMKTDHFAPWLSLPGVMAFSSNPAAAHIALDVGAQRQQDWLRKMGFFSPAPIELP
EAGRPIVPQTRNWGISTVMTVGFGHGVAEPPLAIVRGTAATVNGGLLLHPTLLARDDAGTDAPAAPSSDPSPTEPGAPAA
AGTRVISPEISALLRRILRLDVTQGTGKSAESPGYFVGGKTGTAEKIGPHGGYLKHVNVAAFTSIFPMNAPRYAVYVMLD
SPKATPQTHGWTTAAWNAAPTVSKIVTRIGPMLGLFPNTANAQAIDEALAIPMEPAVPRGYRALGPGNDPGDPRNAPKPP
KTPKKGERHGDMPVADLSTQPRLHAARAERAARSSDPMESRG

Sequences:

>Translated_682_residues
MTNGPTDTPDSMRSGPSRPQRMDVRITGDDLRSRTARERTQSRLLGVSVGFCALFGAVALKLAAATVIMPMEPERRQVAP
QVPTIPKSDPKGMMAGDFALPQVHRASITDRNGQVLAISLPVAQVYANPQEMIDPQDAAQKLKSVLPALDTDETIRRLST
KKQFVYLARDISPMQEVAINNLGIPGIYFEPGERRHYPLGRVAAHIMGAVDIDDHGVAGVERFLDKRLDSDRTPLRLSLD
VRIQAVVRDELAAAKDMFQAIGACAIVMDVNTGEIIAMVSLPDYDANDFGRADPDARFNRAVTGMYEPGSTFKLQTAAMA
LQMGVAHIWDRFSTIPIHIGRFTISDMKTDHFAPWLSLPGVMAFSSNPAAAHIALDVGAQRQQDWLRKMGFFSPAPIELP
EAGRPIVPQTRNWGISTVMTVGFGHGVAEPPLAIVRGTAATVNGGLLLHPTLLARDDAGTDAPAAPSSDPSPTEPGAPAA
AGTRVISPEISALLRRILRLDVTQGTGKSAESPGYFVGGKTGTAEKIGPHGGYLKHVNVAAFTSIFPMNAPRYAVYVMLD
SPKATPQTHGWTTAAWNAAPTVSKIVTRIGPMLGLFPNTANAQAIDEALAIPMEPAVPRGYRALGPGNDPGDPRNAPKPP
KTPKKGERHGDMPVADLSTQPRLHAARAERAARSSDPMESRG
>Mature_681_residues
TNGPTDTPDSMRSGPSRPQRMDVRITGDDLRSRTARERTQSRLLGVSVGFCALFGAVALKLAAATVIMPMEPERRQVAPQ
VPTIPKSDPKGMMAGDFALPQVHRASITDRNGQVLAISLPVAQVYANPQEMIDPQDAAQKLKSVLPALDTDETIRRLSTK
KQFVYLARDISPMQEVAINNLGIPGIYFEPGERRHYPLGRVAAHIMGAVDIDDHGVAGVERFLDKRLDSDRTPLRLSLDV
RIQAVVRDELAAAKDMFQAIGACAIVMDVNTGEIIAMVSLPDYDANDFGRADPDARFNRAVTGMYEPGSTFKLQTAAMAL
QMGVAHIWDRFSTIPIHIGRFTISDMKTDHFAPWLSLPGVMAFSSNPAAAHIALDVGAQRQQDWLRKMGFFSPAPIELPE
AGRPIVPQTRNWGISTVMTVGFGHGVAEPPLAIVRGTAATVNGGLLLHPTLLARDDAGTDAPAAPSSDPSPTEPGAPAAA
GTRVISPEISALLRRILRLDVTQGTGKSAESPGYFVGGKTGTAEKIGPHGGYLKHVNVAAFTSIFPMNAPRYAVYVMLDS
PKATPQTHGWTTAAWNAAPTVSKIVTRIGPMLGLFPNTANAQAIDEALAIPMEPAVPRGYRALGPGNDPGDPRNAPKPPK
TPKKGERHGDMPVADLSTQPRLHAARAERAARSSDPMESRG

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=503, Percent_Identity=30.4174950298211, Blast_Score=202, Evalue=8e-53,
Organism=Escherichia coli, GI1786854, Length=613, Percent_Identity=22.1859706362153, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 73131; Mature: 73000

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNGPTDTPDSMRSGPSRPQRMDVRITGDDLRSRTARERTQSRLLGVSVGFCALFGAVAL
CCCCCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAAATVIMPMEPERRQVAPQVPTIPKSDPKGMMAGDFALPQVHRASITDRNGQVLAISL
HHHHHHEEECCCCHHHHCCCCCCCCCCCCCCCCEECCCCCCCHHHHHCCCCCCCEEEEEC
PVAQVYANPQEMIDPQDAAQKLKSVLPALDTDETIRRLSTKKQFVYLARDISPMQEVAIN
CHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHH
NLGIPGIYFEPGERRHYPLGRVAAHIMGAVDIDDHGVAGVERFLDKRLDSDRTPLRLSLD
CCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
VRIQAVVRDELAAAKDMFQAIGACAIVMDVNTGEIIAMVSLPDYDANDFGRADPDARFNR
EEEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHH
AVTGMYEPGSTFKLQTAAMALQMGVAHIWDRFSTIPIHIGRFTISDMKTDHFAPWLSLPG
HEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHCCCC
VMAFSSNPAAAHIALDVGAQRQQDWLRKMGFFSPAPIELPEAGRPIVPQTRNWGISTVMT
EEEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VGFGHGVAEPPLAIVRGTAATVNGGLLLHPTLLARDDAGTDAPAAPSSDPSPTEPGAPAA
EECCCCCCCCCCEEEECCCEEECCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
AGTRVISPEISALLRRILRLDVTQGTGKSAESPGYFVGGKTGTAEKIGPHGGYLKHVNVA
CCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHCCCCCCCCEEEEHHH
AFTSIFPMNAPRYAVYVMLDSPKATPQTHGWTTAAWNAAPTVSKIVTRIGPMLGLFPNTA
HHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCC
NAQAIDEALAIPMEPAVPRGYRALGPGNDPGDPRNAPKPPKTPKKGERHGDMPVADLSTQ
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRLHAARAERAARSSDPMESRG
CCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TNGPTDTPDSMRSGPSRPQRMDVRITGDDLRSRTARERTQSRLLGVSVGFCALFGAVAL
CCCCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAAATVIMPMEPERRQVAPQVPTIPKSDPKGMMAGDFALPQVHRASITDRNGQVLAISL
HHHHHHEEECCCCHHHHCCCCCCCCCCCCCCCCEECCCCCCCHHHHHCCCCCCCEEEEEC
PVAQVYANPQEMIDPQDAAQKLKSVLPALDTDETIRRLSTKKQFVYLARDISPMQEVAIN
CHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHH
NLGIPGIYFEPGERRHYPLGRVAAHIMGAVDIDDHGVAGVERFLDKRLDSDRTPLRLSLD
CCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
VRIQAVVRDELAAAKDMFQAIGACAIVMDVNTGEIIAMVSLPDYDANDFGRADPDARFNR
EEEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHH
AVTGMYEPGSTFKLQTAAMALQMGVAHIWDRFSTIPIHIGRFTISDMKTDHFAPWLSLPG
HEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHCCCC
VMAFSSNPAAAHIALDVGAQRQQDWLRKMGFFSPAPIELPEAGRPIVPQTRNWGISTVMT
EEEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VGFGHGVAEPPLAIVRGTAATVNGGLLLHPTLLARDDAGTDAPAAPSSDPSPTEPGAPAA
EECCCCCCCCCCEEEECCCEEECCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
AGTRVISPEISALLRRILRLDVTQGTGKSAESPGYFVGGKTGTAEKIGPHGGYLKHVNVA
CCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHCCCCCCCCEEEEHHH
AFTSIFPMNAPRYAVYVMLDSPKATPQTHGWTTAAWNAAPTVSKIVTRIGPMLGLFPNTA
HHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCC
NAQAIDEALAIPMEPAVPRGYRALGPGNDPGDPRNAPKPPKTPKKGERHGDMPVADLSTQ
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRLHAARAERAARSSDPMESRG
CCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]