Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 162148956

GI number: 162148956

Start: 3272403

End: 3273866

Strand: Direct

Name: murE [H]

Synonym: GDI_3186

Alternate gene names: 162148956

Gene position: 3272403-3273866 (Clockwise)

Preceding gene: 162148955

Following gene: 162148957

Centisome position: 82.97

GC content: 74.93

Gene sequence:

>1464_bases
ATGACCCACCCCCTGTCCCAGGTGCTGGCGGCGGCCGGCCTGTCCGCCCCGCCCGGCGCCGACCCGGCGATTACCGGCGT
GACCGCCGACAGCCGGCGGGTGACGCCGGGCATGCTGTTCGTCGCCATTCCCGGCACGCGCGCCGACGGCAGCGCCTTCA
TCCCCGCCGCGATCGCCGCCGGCGCCGCCGCGATCGTCGCACCGCGCGGGACCCCCGCGCCCGGCGTCCCGGTCATCGGG
GTGGACGAACCGCGCCGGGCGCTGGCCCTGCTGGCGGCGTTCCTCGCCGGGGCGCAGCCGGACCATGTCGTCGCCGTCAC
CGGCACCAACGGCAAGACCAGCACCGTCGATTTCCTGCGCCAGATATGGGGCCATCAGCACCGCCGCGCCGCCAGCATCG
GCACGCTGGGGGTGATCGCGGACATCGACCTTCCCGATTGCGGGCCGGTCCTGACCACGCCGGACAGCGTGGGCCTGGCC
ACCCTGCTGGCCGCCATGGCGCGCGGCGGCGTCACCGACGTGGCCATCGAGGCATCGTCCCACGGGCTGGACCAGCACCG
GCTGGACGGGCTGCGCCTGTCGGCGGCCGGCTTTACCAACCTGACCCGAGACCATCTGGACTATCACGGCACGCTGGACG
CCTACCGCGCGGCCAAGCTGCGCCTGTTCGACACGCTGCTGCCGCCGGACGGGCTGATGGCGGCGAATGCGGACATGGAC
ACCGCGACGCTGGACGCGCTACGCGATATTGCCGCGCGGCGGGGACTGGACCTGCGCCTGGTCGGCGAAGCGGGAGATGC
GATCCGCCTGCTGGGCAGCACCCCGCTGCCCGAGGGCCAGCATCTGCGGATCGCGTCGGGCGGGGTTGCGCGCGACATCG
TCCTGGCACTGCCCGGCCGCTTCCAGGCCGACAATGCGCTGCTGGCGGCGGCGCTGGCCGCGCCGGGCGCCGACGGGCTG
GCCCGGGCGATCGACCTGCTGCCTGCGCTGCGCGGCGTGCGCGGACGGATGGAGCGCGCGGCGCTGCTGCCCAACGGGGC
GGCGGCCTATGTCGATTACGCCCATACCCCGGACGCGCTGGCCCGCCTGCTGGACGCGCTGCGCCCGCACGCCTCGGGCC
GGCTGATCGCCGTGTTCGGCGCCGGCGGCGACCGCGATCGCGGCAAGCGCCCGCTGATGGGGCAGGAAGCGACCCGCCGC
GCCGACATCGCCATCGTCACCGACGACAACCCCCGCACCGAGGACCCCGCCTTCATCCGGGGCGAGGTCCTGGCCGGCGC
GCCGGGCGCGATCGAAATCGGCGATCGCGCGCGGGCGATCGCCGAGGGCCTGTCGATGCTGCGGGCCGGCGACGTGCTGG
TGGTGGCCGGCAAGGGCCACGAACAGGGCCAGACCATCGGCACCGCGACGCTGCCCTTCGATGACGCATCGGTCATTCGC
GACCTGGCCGGGGTGCGGGCATGA

Upstream 100 bases:

>100_bases
ATGGCGACATGCCGGTGGCCGACCTGTCCACGCAGCCGCGCCTGCACGCCGCCCGGGCAGAACGGGCCGCGCGGTCCTCC
GACCCGATGGAATCCCGCGG

Downstream 100 bases:

>100_bases
CGGTTCTGTGGACCCGCGCGGCACTGCAGGACGCGACCGGCGGCACGTTCACGGGGCAGGCGCCGGTTGCAGTCACCGGG
GTGTCGATCGACACCCGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTHPLSQVLAAAGLSAPPGADPAITGVTADSRRVTPGMLFVAIPGTRADGSAFIPAAIAAGAAAIVAPRGTPAPGVPVIG
VDEPRRALALLAAFLAGAQPDHVVAVTGTNGKTSTVDFLRQIWGHQHRRAASIGTLGVIADIDLPDCGPVLTTPDSVGLA
TLLAAMARGGVTDVAIEASSHGLDQHRLDGLRLSAAGFTNLTRDHLDYHGTLDAYRAAKLRLFDTLLPPDGLMAANADMD
TATLDALRDIAARRGLDLRLVGEAGDAIRLLGSTPLPEGQHLRIASGGVARDIVLALPGRFQADNALLAAALAAPGADGL
ARAIDLLPALRGVRGRMERAALLPNGAAAYVDYAHTPDALARLLDALRPHASGRLIAVFGAGGDRDRGKRPLMGQEATRR
ADIAIVTDDNPRTEDPAFIRGEVLAGAPGAIEIGDRARAIAEGLSMLRAGDVLVVAGKGHEQGQTIGTATLPFDDASVIR
DLAGVRA

Sequences:

>Translated_487_residues
MTHPLSQVLAAAGLSAPPGADPAITGVTADSRRVTPGMLFVAIPGTRADGSAFIPAAIAAGAAAIVAPRGTPAPGVPVIG
VDEPRRALALLAAFLAGAQPDHVVAVTGTNGKTSTVDFLRQIWGHQHRRAASIGTLGVIADIDLPDCGPVLTTPDSVGLA
TLLAAMARGGVTDVAIEASSHGLDQHRLDGLRLSAAGFTNLTRDHLDYHGTLDAYRAAKLRLFDTLLPPDGLMAANADMD
TATLDALRDIAARRGLDLRLVGEAGDAIRLLGSTPLPEGQHLRIASGGVARDIVLALPGRFQADNALLAAALAAPGADGL
ARAIDLLPALRGVRGRMERAALLPNGAAAYVDYAHTPDALARLLDALRPHASGRLIAVFGAGGDRDRGKRPLMGQEATRR
ADIAIVTDDNPRTEDPAFIRGEVLAGAPGAIEIGDRARAIAEGLSMLRAGDVLVVAGKGHEQGQTIGTATLPFDDASVIR
DLAGVRA
>Mature_486_residues
THPLSQVLAAAGLSAPPGADPAITGVTADSRRVTPGMLFVAIPGTRADGSAFIPAAIAAGAAAIVAPRGTPAPGVPVIGV
DEPRRALALLAAFLAGAQPDHVVAVTGTNGKTSTVDFLRQIWGHQHRRAASIGTLGVIADIDLPDCGPVLTTPDSVGLAT
LLAAMARGGVTDVAIEASSHGLDQHRLDGLRLSAAGFTNLTRDHLDYHGTLDAYRAAKLRLFDTLLPPDGLMAANADMDT
ATLDALRDIAARRGLDLRLVGEAGDAIRLLGSTPLPEGQHLRIASGGVARDIVLALPGRFQADNALLAAALAAPGADGLA
RAIDLLPALRGVRGRMERAALLPNGAAAYVDYAHTPDALARLLDALRPHASGRLIAVFGAGGDRDRGKRPLMGQEATRRA
DIAIVTDDNPRTEDPAFIRGEVLAGAPGAIEIGDRARAIAEGLSMLRAGDVLVVAGKGHEQGQTIGTATLPFDDASVIRD
LAGVRA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=488, Percent_Identity=38.5245901639344, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1786274, Length=292, Percent_Identity=29.4520547945205, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 49911; Mature: 49779

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPLSQVLAAAGLSAPPGADPAITGVTADSRRVTPGMLFVAIPGTRADGSAFIPAAIAA
CCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEECHHHHHC
GAAAIVAPRGTPAPGVPVIGVDEPRRALALLAAFLAGAQPDHVVAVTGTNGKTSTVDFLR
CCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
QIWGHQHRRAASIGTLGVIADIDLPDCGPVLTTPDSVGLATLLAAMARGGVTDVAIEASS
HHHCCHHHHHHCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEECCC
HGLDQHRLDGLRLSAAGFTNLTRDHLDYHGTLDAYRAAKLRLFDTLLPPDGLMAANADMD
CCCCHHHCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCC
TATLDALRDIAARRGLDLRLVGEAGDAIRLLGSTPLPEGQHLRIASGGVARDIVLALPGR
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCC
FQADNALLAAALAAPGADGLARAIDLLPALRGVRGRMERAALLPNGAAAYVDYAHTPDAL
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
ARLLDALRPHASGRLIAVFGAGGDRDRGKRPLMGQEATRRADIAIVTDDNPRTEDPAFIR
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCCCEEEEECCCCCCCCCCEEE
GEVLAGAPGAIEIGDRARAIAEGLSMLRAGDVLVVAGKGHEQGQTIGTATLPFDDASVIR
EEEEECCCCCEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCHHHHHH
DLAGVRA
HHHHCCC
>Mature Secondary Structure 
THPLSQVLAAAGLSAPPGADPAITGVTADSRRVTPGMLFVAIPGTRADGSAFIPAAIAA
CCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEECHHHHHC
GAAAIVAPRGTPAPGVPVIGVDEPRRALALLAAFLAGAQPDHVVAVTGTNGKTSTVDFLR
CCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
QIWGHQHRRAASIGTLGVIADIDLPDCGPVLTTPDSVGLATLLAAMARGGVTDVAIEASS
HHHCCHHHHHHCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEECCC
HGLDQHRLDGLRLSAAGFTNLTRDHLDYHGTLDAYRAAKLRLFDTLLPPDGLMAANADMD
CCCCHHHCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCC
TATLDALRDIAARRGLDLRLVGEAGDAIRLLGSTPLPEGQHLRIASGGVARDIVLALPGR
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCC
FQADNALLAAALAAPGADGLARAIDLLPALRGVRGRMERAALLPNGAAAYVDYAHTPDAL
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
ARLLDALRPHASGRLIAVFGAGGDRDRGKRPLMGQEATRRADIAIVTDDNPRTEDPAFIR
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCCCEEEEECCCCCCCCCCEEE
GEVLAGAPGAIEIGDRARAIAEGLSMLRAGDVLVVAGKGHEQGQTIGTATLPFDDASVIR
EEEEECCCCCEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCHHHHHH
DLAGVRA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]