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The map label for this gene is leuC [H]

Identifier: 162148858

GI number: 162148858

Start: 3182663

End: 3184114

Strand: Reverse

Name: leuC [H]

Synonym: GDI_3087

Alternate gene names: 162148858

Gene position: 3184114-3182663 (Counterclockwise)

Preceding gene: 162148859

Following gene: 162148857

Centisome position: 80.73

GC content: 68.87

Gene sequence:

>1452_bases
ATGGCGGCGCTGGCGTATCCGATGACGAAGAGAAAGGAAGAAAGACCGATGGCCGCACGCACGCTGTTCGACAAGATCTG
GGACAGTCATGTCGTCGAACGGCTTGAGGACGGCACGGCCATTCTCTACATCGACCGCCACCTGGTGCATGAAGTCACCA
GCCCCCAGGCGTTCGAGGGGCTGCGCGCCAGCGGCCGGCGCCTGCGCCGCCCGGACGCCACCATCGCGGTGGTGGACCAT
AACGTGCCGACGTCGGACCGTACCCGGCCGATCGAGGAACCGGAAAGCCGCAACCAGGTCGAAACGCTGGAGCGCAATGT
CCGGGAATTTGGCGTTCCCTATTTCCCGCTGCTGTCCGAACGCCAGGGCATCGTACATGTCGTGGGGCCGGAACAGGGGA
TTTCGCTGCCTGGCATGACCATCGTCTGCGGCGACAGCCATACGTCCACCCACGGCGCGCTGGGTGCGCTGGCGTTCGGC
ATCGGCACGTCCGAGGTCGAGCATGTCATGGCGACCCAGACGCTGCTCCAGAAGCCCGCGCGGAACATGAAGGTGGTGGT
CGAGGGCCCGCTGGGCGCGGGGGTGACGGCCAAGGACGTGATGCTGGCGATCATCGGCCGCATCGGCACGGCGGGCGGCA
CCGGGCATGTGATCGAATTCGCGGGATCGACCATTCGCGGGCTGGACATGGCCGGCCGGATGACGATCTGCAACATGGCG
ATCGAAGCCGGCGCCCGCGCCGGCCTGGTCGCGCCCGACCAGACGACGTTCGACTATGTGCGCGGCCGCCCCTTCGCCCC
GCAGGGCGAGGCGTTCGACCAGGCGGTGGCCTGGTGGAAGACGCTGCCTGCGGACGAGGGCGCGCAGTACGACAAGGTCG
TCGAATTGCGGGCCGAGGACATTCCGCCGGTCCTGACCTGGGGCACCAGCCCGGAAACCGTCATTCCGGTGACGGGCACG
GTCCCCGATCCGCAGGCCGAGCCCGACGAGGCCCGGCGCGGGCAGATGCGGCGCATGCTGGACTATATGGGCCTGCAGGC
CGGGCAGCCGATTGCCGGCGTGCCGGTGGATGTCGTCTTCATCGGGTCCTGCACCAACAGCCGGATCGAGGACCTGCGTT
CGGCGGCCGCGGTGGTGGCGGGCCGTCATGTCGCGCCGGGCGTGCGCGCGATGGTGGTGCCTGGTTCGGGCATCGTGAAG
GCCCAGGCCGAGCAGGAGGGGCTGGACCGCATCTTCCGCGATGCCGGCTTCGAATGGCGCGAGGCAGGCTGTTCGATGTG
CCTGGGCATGAACCCGGACAAGCTGACCCCCGGCCAGCGCTGCGCCTCGACCTCGAACCGCAATTTCGAGGGACGGCAGG
GCCCGGGCGGCCGCACCCACCTGCTGTCGCCCGCCATGGCGGCGGCGGCGGCGGTCACGGGACATCTGACAGACGTGCGG
GAGCTGATCTGA

Upstream 100 bases:

>100_bases
CGAGCGCGCGCGTGAGACGGTGTCGGCCGAGGCCTGAGCCTCTCCGCCCGCGACGCGGACAGGACCGGGTTCGTCGCTCC
GCCATTCGTCCCCGTGACGG

Downstream 100 bases:

>100_bases
GCCATGGAGAAATTCACCGTCCTGACCGCGATCGCGGCGCCGCTGCCCGAAGCGAACATCGACACGGACAAGATCATTCC
CGCCCGGTTCCTGAAGACCA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAALAYPMTKRKEERPMAARTLFDKIWDSHVVERLEDGTAILYIDRHLVHEVTSPQAFEGLRASGRRLRRPDATIAVVDH
NVPTSDRTRPIEEPESRNQVETLERNVREFGVPYFPLLSERQGIVHVVGPEQGISLPGMTIVCGDSHTSTHGALGALAFG
IGTSEVEHVMATQTLLQKPARNMKVVVEGPLGAGVTAKDVMLAIIGRIGTAGGTGHVIEFAGSTIRGLDMAGRMTICNMA
IEAGARAGLVAPDQTTFDYVRGRPFAPQGEAFDQAVAWWKTLPADEGAQYDKVVELRAEDIPPVLTWGTSPETVIPVTGT
VPDPQAEPDEARRGQMRRMLDYMGLQAGQPIAGVPVDVVFIGSCTNSRIEDLRSAAAVVAGRHVAPGVRAMVVPGSGIVK
AQAEQEGLDRIFRDAGFEWREAGCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGPGGRTHLLSPAMAAAAAVTGHLTDVR
ELI

Sequences:

>Translated_483_residues
MAALAYPMTKRKEERPMAARTLFDKIWDSHVVERLEDGTAILYIDRHLVHEVTSPQAFEGLRASGRRLRRPDATIAVVDH
NVPTSDRTRPIEEPESRNQVETLERNVREFGVPYFPLLSERQGIVHVVGPEQGISLPGMTIVCGDSHTSTHGALGALAFG
IGTSEVEHVMATQTLLQKPARNMKVVVEGPLGAGVTAKDVMLAIIGRIGTAGGTGHVIEFAGSTIRGLDMAGRMTICNMA
IEAGARAGLVAPDQTTFDYVRGRPFAPQGEAFDQAVAWWKTLPADEGAQYDKVVELRAEDIPPVLTWGTSPETVIPVTGT
VPDPQAEPDEARRGQMRRMLDYMGLQAGQPIAGVPVDVVFIGSCTNSRIEDLRSAAAVVAGRHVAPGVRAMVVPGSGIVK
AQAEQEGLDRIFRDAGFEWREAGCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGPGGRTHLLSPAMAAAAAVTGHLTDVR
ELI
>Mature_482_residues
AALAYPMTKRKEERPMAARTLFDKIWDSHVVERLEDGTAILYIDRHLVHEVTSPQAFEGLRASGRRLRRPDATIAVVDHN
VPTSDRTRPIEEPESRNQVETLERNVREFGVPYFPLLSERQGIVHVVGPEQGISLPGMTIVCGDSHTSTHGALGALAFGI
GTSEVEHVMATQTLLQKPARNMKVVVEGPLGAGVTAKDVMLAIIGRIGTAGGTGHVIEFAGSTIRGLDMAGRMTICNMAI
EAGARAGLVAPDQTTFDYVRGRPFAPQGEAFDQAVAWWKTLPADEGAQYDKVVELRAEDIPPVLTWGTSPETVIPVTGTV
PDPQAEPDEARRGQMRRMLDYMGLQAGQPIAGVPVDVVFIGSCTNSRIEDLRSAAAVVAGRHVAPGVRAMVVPGSGIVKA
QAEQEGLDRIFRDAGFEWREAGCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGPGGRTHLLSPAMAAAAAVTGHLTDVRE
LI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=367, Percent_Identity=28.6103542234332, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI8659555, Length=453, Percent_Identity=26.0485651214128, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=27.8947368421053, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=64.4396551724138, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI1787531, Length=448, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=28.6501377410468, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=28.6501377410468, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=27.7602523659306, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=456, Percent_Identity=25.6578947368421, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=62.3404255319149, Blast_Score=619, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6320440, Length=426, Percent_Identity=26.7605633802817, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=28.8461538461538, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=501, Percent_Identity=26.9461077844311, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281365315, Length=488, Percent_Identity=26.4344262295082, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=488, Percent_Identity=26.4344262295082, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076999, Length=368, Percent_Identity=28.5326086956522, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571643, Length=473, Percent_Identity=25.369978858351, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.1455525606469, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51913; Mature: 51782

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALAYPMTKRKEERPMAARTLFDKIWDSHVVERLEDGTAILYIDRHLVHEVTSPQAFEG
CCCCCCCHHHCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCHHHHH
LRASGRRLRRPDATIAVVDHNVPTSDRTRPIEEPESRNQVETLERNVREFGVPYFPLLSE
HHHCCHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
RQGIVHVVGPEQGISLPGMTIVCGDSHTSTHGALGALAFGIGTSEVEHVMATQTLLQKPA
CCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
RNMKVVVEGPLGAGVTAKDVMLAIIGRIGTAGGTGHVIEFAGSTIRGLDMAGRMTICNMA
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCCCHHHHHHH
IEAGARAGLVAPDQTTFDYVRGRPFAPQGEAFDQAVAWWKTLPADEGAQYDKVVELRAED
HHCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
IPPVLTWGTSPETVIPVTGTVPDPQAEPDEARRGQMRRMLDYMGLQAGQPIAGVPVDVVF
CCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
IGSCTNSRIEDLRSAAAVVAGRHVAPGVRAMVVPGSGIVKAQAEQEGLDRIFRDAGFEWR
EECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHCCCHHH
EAGCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGPGGRTHLLSPAMAAAAAVTGHLTDVR
HCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
ELI
HHC
>Mature Secondary Structure 
AALAYPMTKRKEERPMAARTLFDKIWDSHVVERLEDGTAILYIDRHLVHEVTSPQAFEG
CCCCCCHHHCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCHHHHH
LRASGRRLRRPDATIAVVDHNVPTSDRTRPIEEPESRNQVETLERNVREFGVPYFPLLSE
HHHCCHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
RQGIVHVVGPEQGISLPGMTIVCGDSHTSTHGALGALAFGIGTSEVEHVMATQTLLQKPA
CCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
RNMKVVVEGPLGAGVTAKDVMLAIIGRIGTAGGTGHVIEFAGSTIRGLDMAGRMTICNMA
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCCCHHHHHHH
IEAGARAGLVAPDQTTFDYVRGRPFAPQGEAFDQAVAWWKTLPADEGAQYDKVVELRAED
HHCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
IPPVLTWGTSPETVIPVTGTVPDPQAEPDEARRGQMRRMLDYMGLQAGQPIAGVPVDVVF
CCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
IGSCTNSRIEDLRSAAAVVAGRHVAPGVRAMVVPGSGIVKAQAEQEGLDRIFRDAGFEWR
EECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHCCCHHH
EAGCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGPGGRTHLLSPAMAAAAAVTGHLTDVR
HCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
ELI
HHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA