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The map label for this gene is nouE [H]

Identifier: 162148807

GI number: 162148807

Start: 3128017

End: 3128676

Strand: Reverse

Name: nouE [H]

Synonym: GDI_3036

Alternate gene names: 162148807

Gene position: 3128676-3128017 (Counterclockwise)

Preceding gene: 162148808

Following gene: 162148806

Centisome position: 79.32

GC content: 66.97

Gene sequence:

>660_bases
GTGAAAGAGATGTCCGCTCACTCCCCCATTGACCAGATCGAGCAGCCTTCCTCCTTCGCCTTCGATGCCGAGAGCGAGGC
CGAGATCGCCACGATCCTGGTCAAGTACCCGCCCGAGCGCAAAGCCAGCGGCACGCTGCCGCTGCTCTATGTCGCGCAGA
AGCAGATGGGCCGCGTCACCGGCAGCGCCTGGGTGCCCCGGGTGGCGATGGACGAGATCGCCCGCCGGCTGGAGGTCGCG
CCGATCCGCGTCTACGAGGTTGCGACCTTCTACCTGATGTTCAACACCAAGCCGATCGGCCGCTATCACCTGCAGGTCTG
CACCACCACCTCGTGCTGGCTGCGCGGTTCGGACGACGTGGTCGCGGCCTGCAAGAAGGCCACCGGCATCTCCGCCTTCG
GCCAGTCCAGCGCCGACGGACTGTTCACCCTGACCGAGGTCGAATGCCTGGGGGGATGTTCCAACGCGCCGATCCTGCAG
GTGGATGACGATTTCTACGAGGATCTGGACGGACCGCGCACGATCGAACTGATCGAGGCGCTGCGGCGCGGCGATCGCCC
GAAGCCGGGCCCGACCATCGATAGAATGGGTGCCGCCCCCGCGGGGGGGCGCAAGACGCTGACGGACAGTCCGGCGGACA
GCCGGTCGGACCAGGGTTGA

Upstream 100 bases:

>100_bases
CCCATCTGCAGGCCATCGACGAGATGTCGCGCCGCCACATGCTGGCCGACGCGGTGGCGATCATCGGGTCGCTGGACCTG
GTGTTCGGCGAGATTGACAG

Downstream 100 bases:

>100_bases
GCGGGAGAGACGGCAATGCTTCAGGACAAGGACCGGATCTTCACCAACCTGTATGGTCAGCAGGACTGGCGTCTGGCCGG
CGCCCGGGCGCGCGGCGACT

Product: NADH-quinone oxidoreductase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKEMSAHSPIDQIEQPSSFAFDAESEAEIATILVKYPPERKASGTLPLLYVAQKQMGRVTGSAWVPRVAMDEIARRLEVA
PIRVYEVATFYLMFNTKPIGRYHLQVCTTTSCWLRGSDDVVAACKKATGISAFGQSSADGLFTLTEVECLGGCSNAPILQ
VDDDFYEDLDGPRTIELIEALRRGDRPKPGPTIDRMGAAPAGGRKTLTDSPADSRSDQG

Sequences:

>Translated_219_residues
MKEMSAHSPIDQIEQPSSFAFDAESEAEIATILVKYPPERKASGTLPLLYVAQKQMGRVTGSAWVPRVAMDEIARRLEVA
PIRVYEVATFYLMFNTKPIGRYHLQVCTTTSCWLRGSDDVVAACKKATGISAFGQSSADGLFTLTEVECLGGCSNAPILQ
VDDDFYEDLDGPRTIELIEALRRGDRPKPGPTIDRMGAAPAGGRKTLTDSPADSRSDQG
>Mature_219_residues
MKEMSAHSPIDQIEQPSSFAFDAESEAEIATILVKYPPERKASGTLPLLYVAQKQMGRVTGSAWVPRVAMDEIARRLEVA
PIRVYEVATFYLMFNTKPIGRYHLQVCTTTSCWLRGSDDVVAACKKATGISAFGQSSADGLFTLTEVECLGGCSNAPILQ
VDDDFYEDLDGPRTIELIEALRRGDRPKPGPTIDRMGAAPAGGRKTLTDSPADSRSDQG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=195, Percent_Identity=48.2051282051282, Blast_Score=195, Evalue=2e-50,
Organism=Escherichia coli, GI1788621, Length=172, Percent_Identity=34.3023255813954, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=194, Percent_Identity=45.360824742268, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI18859877, Length=193, Percent_Identity=44.559585492228, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355353, Length=200, Percent_Identity=37.5, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 23804; Mature: 23804

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMSAHSPIDQIEQPSSFAFDAESEAEIATILVKYPPERKASGTLPLLYVAQKQMGRVT
CCCCCCCCCHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCHHEEEHHHHHCCCC
GSAWVPRVAMDEIARRLEVAPIRVYEVATFYLMFNTKPIGRYHLQVCTTTSCWLRGSDDV
CCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCEEEEEEEECCEEEEECCCHH
VAACKKATGISAFGQSSADGLFTLTEVECLGGCSNAPILQVDDDFYEDLDGPRTIELIEA
HHHHHHHCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEECCHHHHHCCCCCHHHHHHH
LRRGDRPKPGPTIDRMGAAPAGGRKTLTDSPADSRSDQG
HHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKEMSAHSPIDQIEQPSSFAFDAESEAEIATILVKYPPERKASGTLPLLYVAQKQMGRVT
CCCCCCCCCHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCHHEEEHHHHHCCCC
GSAWVPRVAMDEIARRLEVAPIRVYEVATFYLMFNTKPIGRYHLQVCTTTSCWLRGSDDV
CCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCEEEEEEEECCEEEEECCCHH
VAACKKATGISAFGQSSADGLFTLTEVECLGGCSNAPILQVDDDFYEDLDGPRTIELIEA
HHHHHHHCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEECCHHHHHCCCCCHHHHHHH
LRRGDRPKPGPTIDRMGAAPAGGRKTLTDSPADSRSDQG
HHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]