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The map label for this gene is tsf [H]

Identifier: 162148789

GI number: 162148789

Start: 3106544

End: 3107458

Strand: Reverse

Name: tsf [H]

Synonym: GDI_3018

Alternate gene names: 162148789

Gene position: 3107458-3106544 (Counterclockwise)

Preceding gene: 162148790

Following gene: 162148788

Centisome position: 78.79

GC content: 68.96

Gene sequence:

>915_bases
GTGACGCATATGGCGGAGATTACCGCAGCCCTGGTGAAGGAACTTCGCGAGAAGACCGGTGCCGGCATGATGGACTGCAA
GAAGGCCCTGAACGAGGCCGGCGGCGAGATCGAGGGCGCGATCGACTGGCTGCGCACCAAGGGCCTGGCGGCGGCCGCGA
AGAAGTCGGGCCGCGTGACGGCCGAGGGCCTGGTGGCCGTGGCCTCGGCCCCCCGCGCTGCCGCGATGGTCGAAGTGAAC
GCCGAAACCGACTTCGTCGCGCGCAACGAGAGCTTCCAGGCGTTCGTCGAGGCCGTGGCCCGCGCTGCGCTGACCGCCGG
CGAGGACCTGGAGAAGATCAAGGCCGTGGTGCTGGACAGCGGCCGCACCGTCGCTGACGAGCTGACCCACCTGATCGCGA
CGATCGGCGAGAACATGTCGATCCGCCGCGCCCGCGTGCTGAGCGTGCCGTCGGGCGTCGTCGCCAGCTACATCCATTCC
GCCGTCCGTCCGGGCCTGGGCAAGATCGGCGTCCTGGCCGCCATCGAAGCCCCGTCCGAGAGCGAGGCGCTGGAACTGCT
GGGCCGCCAGGTCGGCATGCATGTCGCCGCCACCCGCCCGGCCGCGCTGGACATCGACAGCGTCGATCCCGCGTCGCTGG
AGCGCGAGCGTGCGGTGCTGGTCGAGCAGGCCCGTGCCTCCGGCAAGCCCGAAGCGATCATCGAGAAGATGGTCGAAGGC
CGCATCCGCAAGTTCTACGAGGAAGTGGTCCTGCTGGAGCAGGTCTGGGTGCATGACGGCGAAAGCACCGTCCGCAAGGT
CGTCCAGAAGGCCGGCGCCAAGCTGAGCGGCTTCGATCGCTTCCAGCTCGGTGAAGGCATCGAGAAGGAAGAGAACGACT
TCGCGGCCGAGGTCGCCAAGGCGGCCGGCGTCTGA

Upstream 100 bases:

>100_bases
CCACCCCGCGCCGCCCGGGCGGCGCGGGGTGGGATCTTCACGATGACAGATGCAAGGGCGCGCCCTGCCGTCACGGCAGG
GCGCGCGCCTGCCTTGAGGA

Downstream 100 bases:

>100_bases
CCGGCACGCGACCCTCTGATCGCAGCCTGAACGGGGCGGGGCCTTCCTGATGGAATGGGAGGCACCCGCCCTTGTGCTGT
CTGCGTCCCCCTATGGCGAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTHMAEITAALVKELREKTGAGMMDCKKALNEAGGEIEGAIDWLRTKGLAAAAKKSGRVTAEGLVAVASAPRAAAMVEVN
AETDFVARNESFQAFVEAVARAALTAGEDLEKIKAVVLDSGRTVADELTHLIATIGENMSIRRARVLSVPSGVVASYIHS
AVRPGLGKIGVLAAIEAPSESEALELLGRQVGMHVAATRPAALDIDSVDPASLERERAVLVEQARASGKPEAIIEKMVEG
RIRKFYEEVVLLEQVWVHDGESTVRKVVQKAGAKLSGFDRFQLGEGIEKEENDFAAEVAKAAGV

Sequences:

>Translated_304_residues
MTHMAEITAALVKELREKTGAGMMDCKKALNEAGGEIEGAIDWLRTKGLAAAAKKSGRVTAEGLVAVASAPRAAAMVEVN
AETDFVARNESFQAFVEAVARAALTAGEDLEKIKAVVLDSGRTVADELTHLIATIGENMSIRRARVLSVPSGVVASYIHS
AVRPGLGKIGVLAAIEAPSESEALELLGRQVGMHVAATRPAALDIDSVDPASLERERAVLVEQARASGKPEAIIEKMVEG
RIRKFYEEVVLLEQVWVHDGESTVRKVVQKAGAKLSGFDRFQLGEGIEKEENDFAAEVAKAAGV
>Mature_303_residues
THMAEITAALVKELREKTGAGMMDCKKALNEAGGEIEGAIDWLRTKGLAAAAKKSGRVTAEGLVAVASAPRAAAMVEVNA
ETDFVARNESFQAFVEAVARAALTAGEDLEKIKAVVLDSGRTVADELTHLIATIGENMSIRRARVLSVPSGVVASYIHSA
VRPGLGKIGVLAAIEAPSESEALELLGRQVGMHVAATRPAALDIDSVDPASLERERAVLVEQARASGKPEAIIEKMVEGR
IRKFYEEVVLLEQVWVHDGESTVRKVVQKAGAKLSGFDRFQLGEGIEKEENDFAAEVAKAAGV

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=226, Percent_Identity=35.8407079646018, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291084500, Length=247, Percent_Identity=33.1983805668016, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI291084502, Length=127, Percent_Identity=38.5826771653543, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI291084498, Length=105, Percent_Identity=40.9523809523809, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786366, Length=298, Percent_Identity=45.9731543624161, Blast_Score=220, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17561440, Length=295, Percent_Identity=25.4237288135593, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921466, Length=298, Percent_Identity=29.1946308724832, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32220; Mature: 32089

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHMAEITAALVKELREKTGAGMMDCKKALNEAGGEIEGAIDWLRTKGLAAAAKKSGRVT
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCC
AEGLVAVASAPRAAAMVEVNAETDFVARNESFQAFVEAVARAALTAGEDLEKIKAVVLDS
HHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
GRTVADELTHLIATIGENMSIRRARVLSVPSGVVASYIHSAVRPGLGKIGVLAAIEAPSE
CCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHEEEECCCCH
SEALELLGRQVGMHVAATRPAALDIDSVDPASLERERAVLVEQARASGKPEAIIEKMVEG
HHHHHHHHHHHCCHHEECCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RIRKFYEEVVLLEQVWVHDGESTVRKVVQKAGAKLSGFDRFQLGEGIEKEENDFAAEVAK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
AAGV
HCCC
>Mature Secondary Structure 
THMAEITAALVKELREKTGAGMMDCKKALNEAGGEIEGAIDWLRTKGLAAAAKKSGRVT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCC
AEGLVAVASAPRAAAMVEVNAETDFVARNESFQAFVEAVARAALTAGEDLEKIKAVVLDS
HHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
GRTVADELTHLIATIGENMSIRRARVLSVPSGVVASYIHSAVRPGLGKIGVLAAIEAPSE
CCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHEEEECCCCH
SEALELLGRQVGMHVAATRPAALDIDSVDPASLERERAVLVEQARASGKPEAIIEKMVEG
HHHHHHHHHHHCCHHEECCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RIRKFYEEVVLLEQVWVHDGESTVRKVVQKAGAKLSGFDRFQLGEGIEKEENDFAAEVAK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
AAGV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA