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The map label for this gene is gatA [H]

Identifier: 162148750

GI number: 162148750

Start: 3067301

End: 3068785

Strand: Reverse

Name: gatA [H]

Synonym: GDI_2978

Alternate gene names: 162148750

Gene position: 3068785-3067301 (Counterclockwise)

Preceding gene: 162148751

Following gene: 162148749

Centisome position: 77.81

GC content: 69.63

Gene sequence:

>1485_bases
ATGCTGACCGAACTGACGATCGCCGCGGCCCGGGACGGGCTGGGGCGCCGGGAATTTTCCGCCGTCGAACTGGTCGGCGC
CCACGTGGCGGCCATCGAGAAGCTGAACGGCCGGCTGAACGCCTTCATCACCGTCACCGCCCCGCAGGCGCTGGACGCCG
CGCGCCGCGCCGATGCGATCCTGGCCGGTGGGGATGCGCCCGCGCCGCTGACCGGCATTCCGCTGGGGATCAAGGACCTG
TTCTGCACCGACGGCGTGCGCACCACCGCCGCCAGCCGGATGCTGGAGGATTTCGTGCCCCCGTACGAAAGCACGGTGAC
GGCGAACCTGCTGCGCGACGGTGCGGTGTTCGTGGGCAAGACCAATTTGGACGAATTCGCGATGGGTTCGGCCAACATCA
ACTCGGCCTTCGGCGCGGTGGAAAATCCGTGGAAGCGCGCGGGTGACACCGACACCGTGCTGGTCCCCGGCGGGTCGTCG
GGCGGGTCGGCGGCGGCGGTGGCCGCCGGCCTGGCCATGGGCGCCACCGGCACCGACACCGGCGGGTCGATCCGCCAGCC
CGCGTCCTATTGCGGCATCGCGGGGATCAAGCCGACCTATGGACGGTGCAGCCGCTGGGGCACCATCGCCTTCGCCAGTT
CCCTGGACCAGGCGGGCCCGATGGCGCGCACGGTCGAGGATTGCGCCATCCTGCTGCAGTCGATGGCGGGGTTCGATCCG
CGAGACAGCACCAGCGTCGATATCGCGGTGCCGGACTACCGCGCCGCCTGCGGCCGGTCGCTGAAGGGCATGAAGGTCGG
CATTCCCGCCGAATACCGCGTCGCCGGCATGCCCGCCGAAATCGAGGCGGCGTGGGAGCAGGGCATCGCCTGGCTGCGCG
ACGCGGGGTGCGAGATCGTGGAGGTGTCGCTGCCCTACACCAAATACGGGTTGGCGACCTATTATATCGTGGCGCCGGCG
GAATGTTCGTCCAACCTGGCGCGTTATGACGGCGTGCGCTTCGGCCGCCGCGTGCCGGCGGCGTCTCTGGACGAGCTGTA
CGAGGCGACGCGGCGCGAGGGATTCGGGGCCGAGGTCCGGCGCCGCATCCTGATGGGGACCTACGTCCTCTCGGCCGGCT
ATTACGACGCGTACTATCTGCGCGCGCAGAAGGTCCGCAACCTGATCCGACGGGATTTCGAACAGGCGTTCCGCACGGTC
GATGTCCTGCTGACGCCGACCGCGCCGTCGCCGGCCTTCGCGCAGGGCGAAAAGATGGATGATCCGGTACAGATGTATCT
GAACGACGTATTTACCGTGCCCGCCAGCATGGCCGGCGTACCCGCCATGTCGGTCCCGGCGGGGCTCAGCACCACGGGCC
TGCCGCTGGGGCTGCAGGTGATCGGCCGGTCGTTCGACGAGGAATCGGTGCTTGCGGCCGCCAGCGCCATCGAACGGGCG
GCGGCCTTCACGCACCGTCCCGCCCTGCGCGCGGAGGTGGTGTGA

Upstream 100 bases:

>100_bases
GCGAGGACGTGGTGACCGACGGGGCCGCGCGCGATGCCGTCCTGTCGAACGCGCCCGACGCGGCGGGGCCGTTCTATACC
GTGCCGAAGGTGGTTGAATA

Downstream 100 bases:

>100_bases
TGACCTATCTGGTCGAAGGCCGTACCGGCCCCTGGGAACTGGTCGTGGGGCTGGAAGTCCACGCCCAGGTCATCAGCCGG
TCCAAGCTGTTCTCGGGCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MLTELTIAAARDGLGRREFSAVELVGAHVAAIEKLNGRLNAFITVTAPQALDAARRADAILAGGDAPAPLTGIPLGIKDL
FCTDGVRTTAASRMLEDFVPPYESTVTANLLRDGAVFVGKTNLDEFAMGSANINSAFGAVENPWKRAGDTDTVLVPGGSS
GGSAAAVAAGLAMGATGTDTGGSIRQPASYCGIAGIKPTYGRCSRWGTIAFASSLDQAGPMARTVEDCAILLQSMAGFDP
RDSTSVDIAVPDYRAACGRSLKGMKVGIPAEYRVAGMPAEIEAAWEQGIAWLRDAGCEIVEVSLPYTKYGLATYYIVAPA
ECSSNLARYDGVRFGRRVPAASLDELYEATRREGFGAEVRRRILMGTYVLSAGYYDAYYLRAQKVRNLIRRDFEQAFRTV
DVLLTPTAPSPAFAQGEKMDDPVQMYLNDVFTVPASMAGVPAMSVPAGLSTTGLPLGLQVIGRSFDEESVLAAASAIERA
AAFTHRPALRAEVV

Sequences:

>Translated_494_residues
MLTELTIAAARDGLGRREFSAVELVGAHVAAIEKLNGRLNAFITVTAPQALDAARRADAILAGGDAPAPLTGIPLGIKDL
FCTDGVRTTAASRMLEDFVPPYESTVTANLLRDGAVFVGKTNLDEFAMGSANINSAFGAVENPWKRAGDTDTVLVPGGSS
GGSAAAVAAGLAMGATGTDTGGSIRQPASYCGIAGIKPTYGRCSRWGTIAFASSLDQAGPMARTVEDCAILLQSMAGFDP
RDSTSVDIAVPDYRAACGRSLKGMKVGIPAEYRVAGMPAEIEAAWEQGIAWLRDAGCEIVEVSLPYTKYGLATYYIVAPA
ECSSNLARYDGVRFGRRVPAASLDELYEATRREGFGAEVRRRILMGTYVLSAGYYDAYYLRAQKVRNLIRRDFEQAFRTV
DVLLTPTAPSPAFAQGEKMDDPVQMYLNDVFTVPASMAGVPAMSVPAGLSTTGLPLGLQVIGRSFDEESVLAAASAIERA
AAFTHRPALRAEVV
>Mature_494_residues
MLTELTIAAARDGLGRREFSAVELVGAHVAAIEKLNGRLNAFITVTAPQALDAARRADAILAGGDAPAPLTGIPLGIKDL
FCTDGVRTTAASRMLEDFVPPYESTVTANLLRDGAVFVGKTNLDEFAMGSANINSAFGAVENPWKRAGDTDTVLVPGGSS
GGSAAAVAAGLAMGATGTDTGGSIRQPASYCGIAGIKPTYGRCSRWGTIAFASSLDQAGPMARTVEDCAILLQSMAGFDP
RDSTSVDIAVPDYRAACGRSLKGMKVGIPAEYRVAGMPAEIEAAWEQGIAWLRDAGCEIVEVSLPYTKYGLATYYIVAPA
ECSSNLARYDGVRFGRRVPAASLDELYEATRREGFGAEVRRRILMGTYVLSAGYYDAYYLRAQKVRNLIRRDFEQAFRTV
DVLLTPTAPSPAFAQGEKMDDPVQMYLNDVFTVPASMAGVPAMSVPAGLSTTGLPLGLQVIGRSFDEESVLAAASAIERA
AAFTHRPALRAEVV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=42.2448979591837, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=25, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI166795287, Length=161, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=40.2272727272727, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17537465, Length=488, Percent_Identity=24.3852459016393, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=241, Percent_Identity=30.7053941908714, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=245, Percent_Identity=31.4285714285714, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=247, Percent_Identity=29.9595141700405, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=203, Percent_Identity=30.5418719211823, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=203, Percent_Identity=30.5418719211823, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=36.6197183098592, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=415, Percent_Identity=28.433734939759, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=207, Percent_Identity=31.8840579710145, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648113, Length=459, Percent_Identity=42.0479302832244, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI24652985, Length=510, Percent_Identity=26.2745098039216, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922090, Length=510, Percent_Identity=26.2745098039216, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652981, Length=510, Percent_Identity=26.2745098039216, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652983, Length=510, Percent_Identity=26.2745098039216, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=24.6987951807229, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356731, Length=502, Percent_Identity=26.6932270916335, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI161078093, Length=520, Percent_Identity=26.9230769230769, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=28.9795918367347, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=28.9795918367347, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=28.9795918367347, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648441, Length=187, Percent_Identity=30.4812834224599, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648439, Length=187, Percent_Identity=30.4812834224599, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52123; Mature: 52123

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTELTIAAARDGLGRREFSAVELVGAHVAAIEKLNGRLNAFITVTAPQALDAARRADAI
CCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCEE
LAGGDAPAPLTGIPLGIKDLFCTDGVRTTAASRMLEDFVPPYESTVTANLLRDGAVFVGK
EECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEC
TNLDEFAMGSANINSAFGAVENPWKRAGDTDTVLVPGGSSGGSAAAVAAGLAMGATGTDT
CCCHHHHCCCCCCCHHCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCC
GGSIRQPASYCGIAGIKPTYGRCSRWGTIAFASSLDQAGPMARTVEDCAILLQSMAGFDP
CCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
RDSTSVDIAVPDYRAACGRSLKGMKVGIPAEYRVAGMPAEIEAAWEQGIAWLRDAGCEIV
CCCCEEEEECCCHHHHHCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEE
EVSLPYTKYGLATYYIVAPAECSSNLARYDGVRFGRRVPAASLDELYEATRREGFGAEVR
EEECCCHHHCCEEEEEEECHHHHCCHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHH
RRILMGTYVLSAGYYDAYYLRAQKVRNLIRRDFEQAFRTVDVLLTPTAPSPAFAQGEKMD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
DPVQMYLNDVFTVPASMAGVPAMSVPAGLSTTGLPLGLQVIGRSFDEESVLAAASAIERA
CHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
AAFTHRPALRAEVV
HHHHCCCCCCCCCC
>Mature Secondary Structure
MLTELTIAAARDGLGRREFSAVELVGAHVAAIEKLNGRLNAFITVTAPQALDAARRADAI
CCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCEE
LAGGDAPAPLTGIPLGIKDLFCTDGVRTTAASRMLEDFVPPYESTVTANLLRDGAVFVGK
EECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEC
TNLDEFAMGSANINSAFGAVENPWKRAGDTDTVLVPGGSSGGSAAAVAAGLAMGATGTDT
CCCHHHHCCCCCCCHHCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCC
GGSIRQPASYCGIAGIKPTYGRCSRWGTIAFASSLDQAGPMARTVEDCAILLQSMAGFDP
CCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
RDSTSVDIAVPDYRAACGRSLKGMKVGIPAEYRVAGMPAEIEAAWEQGIAWLRDAGCEIV
CCCCEEEEECCCHHHHHCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEE
EVSLPYTKYGLATYYIVAPAECSSNLARYDGVRFGRRVPAASLDELYEATRREGFGAEVR
EEECCCHHHCCEEEEEEECHHHHCCHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHH
RRILMGTYVLSAGYYDAYYLRAQKVRNLIRRDFEQAFRTVDVLLTPTAPSPAFAQGEKMD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
DPVQMYLNDVFTVPASMAGVPAMSVPAGLSTTGLPLGLQVIGRSFDEESVLAAASAIERA
CHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
AAFTHRPALRAEVV
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA