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The map label for this gene is gatB [H]

Identifier: 162148749

GI number: 162148749

Start: 3065853

End: 3067304

Strand: Reverse

Name: gatB [H]

Synonym: GDI_2977

Alternate gene names: 162148749

Gene position: 3067304-3065853 (Counterclockwise)

Preceding gene: 162148750

Following gene: 162148748

Centisome position: 77.77

GC content: 65.43

Gene sequence:

>1452_bases
GTGATGACCTATCTGGTCGAAGGCCGTACCGGCCCCTGGGAACTGGTCGTGGGGCTGGAAGTCCACGCCCAGGTCATCAG
CCGGTCCAAGCTGTTCTCGGGCGCCTCGGCCTCGTACGGGGGCGAGCCGAACACCCATGTCAGCCTGGTCGATGCGGGAT
TTCCCGGCATGCTGCCGGTCATCAACCGCGAATGCGTGGCCCAGGCGGTGCGGACCGGCCTGGGGCTGAAGGCGCGGATC
AACCTGGAAAGCCGGTTCGACCGCAAGAACTATTTCTATGCGGACCTGCCGACGGGCTATCAGATCAGCCAGTTCGCCCA
TCCGATCATCGGCGAGGGCGCGGTCGAAATCGAACTGGCCGACGGCGCGACCCGCACCATCGGGATCACGCGCCTGCATC
TGGAACAGGATGCCGGCAAGTCGATGCATGACCAGGACCCGACGCGGTCCTTCATCGACCTGAACCGCGCGGGCGTCGCG
CTGATGGAAATCGTCAGCGAACCCGATATCCGCACGCCCGAGGAAGCCGGGGCCTATCTGCGCAAGCTGCGCATGATCCT
GCGCTACCTGGGCACCTGCGACGGCAACATGGAAGAAGGTTCGATGCGCGCCGACGTGAACGTGTCGGTCCGCAAGGCGG
GCGAGCCGTTCCGCACCCGGTGCGAGATCAAGAACGTCAATTCGATTCGCTATGTGATGCAGGCGATCGAGGTCGAGGCC
GTCCGCCAGGTGGAAATCTGGGAAAGCGGCGGCGAGGTCGATCAGGAAACCCGCCTGTTCGACCCCGCGCGGGGCGAGAC
ACGCTCGCTGCGCAGCAAGGAAGACGCCCACGATTACCGCTATTTCCCCGATCCCGACCTGCTGCCGCTGGTGATCGAGG
AAGCGTGGGTCGAATCGCTGGCCGCCGTCCTGCCGGAACTGCCGGATGCCAAGCGCCAGCGCTTCCAGGACGAGTACGGC
ATCCCCCGCTATGATGCCGGGGTGCTGGTGGCCGAACAGGCGACCGCCGATTTCTATGAAACGGTCGCGCGCGGGCGGGA
CCCGCGCCTGGCGGCCAACTGGGTGATCGGCGACCTGTTCGCGGCGTTGAACCGCACCGGCCGCACGATCCAGGACAGCC
CGATCAGCGCAGACGCACTGGGCGGCATGCTGGATCTGGTCACGGACGGCACGATCAACGGCAAGATCGCCAAGGAAGTG
TTCGAGGACATGCTGGAGACCGGCGACCCCGCCGCCGCGATCGTCGAGCGCAAGGGATTGCGGCAGGTCACCGATACCGG
GGCGATCGACGCCGCCGTGCAGGCCGTACTGGCGGCGAACGCGGACAAGCTGGCCGAATACCGTTCGGGCAAGGACAAGC
TGTTCGGATTCTTCGTCGGTCAGGTCATGAAGGCGATGGCCGGCAAGGGCAATCCAGCCCTGGTGAACGAGGCCCTGAAG
AAAGTTCTGTAA

Upstream 100 bases:

>100_bases
TCGGCCGGTCGTTCGACGAGGAATCGGTGCTTGCGGCCGCCAGCGCCATCGAACGGGCGGCGGCCTTCACGCACCGTCCC
GCCCTGCGCGCGGAGGTGGT

Downstream 100 bases:

>100_bases
CCGCCCGCCACGGGCCGGTTTGGGGTTTGACGGCGCTGTGACGACAGTCTACAAGCAGCGCTGCAAACGCCGTGTGTAAA
CACGCTCCTCGGCGGAGGGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MMTYLVEGRTGPWELVVGLEVHAQVISRSKLFSGASASYGGEPNTHVSLVDAGFPGMLPVINRECVAQAVRTGLGLKARI
NLESRFDRKNYFYADLPTGYQISQFAHPIIGEGAVEIELADGATRTIGITRLHLEQDAGKSMHDQDPTRSFIDLNRAGVA
LMEIVSEPDIRTPEEAGAYLRKLRMILRYLGTCDGNMEEGSMRADVNVSVRKAGEPFRTRCEIKNVNSIRYVMQAIEVEA
VRQVEIWESGGEVDQETRLFDPARGETRSLRSKEDAHDYRYFPDPDLLPLVIEEAWVESLAAVLPELPDAKRQRFQDEYG
IPRYDAGVLVAEQATADFYETVARGRDPRLAANWVIGDLFAALNRTGRTIQDSPISADALGGMLDLVTDGTINGKIAKEV
FEDMLETGDPAAAIVERKGLRQVTDTGAIDAAVQAVLAANADKLAEYRSGKDKLFGFFVGQVMKAMAGKGNPALVNEALK
KVL

Sequences:

>Translated_483_residues
MMTYLVEGRTGPWELVVGLEVHAQVISRSKLFSGASASYGGEPNTHVSLVDAGFPGMLPVINRECVAQAVRTGLGLKARI
NLESRFDRKNYFYADLPTGYQISQFAHPIIGEGAVEIELADGATRTIGITRLHLEQDAGKSMHDQDPTRSFIDLNRAGVA
LMEIVSEPDIRTPEEAGAYLRKLRMILRYLGTCDGNMEEGSMRADVNVSVRKAGEPFRTRCEIKNVNSIRYVMQAIEVEA
VRQVEIWESGGEVDQETRLFDPARGETRSLRSKEDAHDYRYFPDPDLLPLVIEEAWVESLAAVLPELPDAKRQRFQDEYG
IPRYDAGVLVAEQATADFYETVARGRDPRLAANWVIGDLFAALNRTGRTIQDSPISADALGGMLDLVTDGTINGKIAKEV
FEDMLETGDPAAAIVERKGLRQVTDTGAIDAAVQAVLAANADKLAEYRSGKDKLFGFFVGQVMKAMAGKGNPALVNEALK
KVL
>Mature_483_residues
MMTYLVEGRTGPWELVVGLEVHAQVISRSKLFSGASASYGGEPNTHVSLVDAGFPGMLPVINRECVAQAVRTGLGLKARI
NLESRFDRKNYFYADLPTGYQISQFAHPIIGEGAVEIELADGATRTIGITRLHLEQDAGKSMHDQDPTRSFIDLNRAGVA
LMEIVSEPDIRTPEEAGAYLRKLRMILRYLGTCDGNMEEGSMRADVNVSVRKAGEPFRTRCEIKNVNSIRYVMQAIEVEA
VRQVEIWESGGEVDQETRLFDPARGETRSLRSKEDAHDYRYFPDPDLLPLVIEEAWVESLAAVLPELPDAKRQRFQDEYG
IPRYDAGVLVAEQATADFYETVARGRDPRLAANWVIGDLFAALNRTGRTIQDSPISADALGGMLDLVTDGTINGKIAKEV
FEDMLETGDPAAAIVERKGLRQVTDTGAIDAAVQAVLAANADKLAEYRSGKDKLFGFFVGQVMKAMAGKGNPALVNEALK
KVL

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=493, Percent_Identity=39.1480730223124, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=342, Percent_Identity=38.3040935672515, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=29.1746641074856, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=37.7551020408163, Blast_Score=338, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53039; Mature: 53039

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTYLVEGRTGPWELVVGLEVHAQVISRSKLFSGASASYGGEPNTHVSLVDAGFPGMLPV
CCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHH
INRECVAQAVRTGLGLKARINLESRFDRKNYFYADLPTGYQISQFAHPIIGEGAVEIELA
HHHHHHHHHHHHCCCCEEEECHHHHCCCCCCEEEECCCCCCHHHHCCHHCCCCEEEEEEC
DGATRTIGITRLHLEQDAGKSMHDQDPTRSFIDLNRAGVALMEIVSEPDIRTPEEAGAYL
CCCCEEEEEEEEEECHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RKLRMILRYLGTCDGNMEEGSMRADVNVSVRKAGEPFRTRCEIKNVNSIRYVMQAIEVEA
HHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHH
VRQVEIWESGGEVDQETRLFDPARGETRSLRSKEDAHDYRYFPDPDLLPLVIEEAWVESL
HHHEEEECCCCCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAVLPELPDAKRQRFQDEYGIPRYDAGVLVAEQATADFYETVARGRDPRLAANWVIGDLF
HHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AALNRTGRTIQDSPISADALGGMLDLVTDGTINGKIAKEVFEDMLETGDPAAAIVERKGL
HHHHHCCCCCCCCCCCHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
RQVTDTGAIDAAVQAVLAANADKLAEYRSGKDKLFGFFVGQVMKAMAGKGNPALVNEALK
HHHHCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KVL
HHC
>Mature Secondary Structure
MMTYLVEGRTGPWELVVGLEVHAQVISRSKLFSGASASYGGEPNTHVSLVDAGFPGMLPV
CCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHH
INRECVAQAVRTGLGLKARINLESRFDRKNYFYADLPTGYQISQFAHPIIGEGAVEIELA
HHHHHHHHHHHHCCCCEEEECHHHHCCCCCCEEEECCCCCCHHHHCCHHCCCCEEEEEEC
DGATRTIGITRLHLEQDAGKSMHDQDPTRSFIDLNRAGVALMEIVSEPDIRTPEEAGAYL
CCCCEEEEEEEEEECHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RKLRMILRYLGTCDGNMEEGSMRADVNVSVRKAGEPFRTRCEIKNVNSIRYVMQAIEVEA
HHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHH
VRQVEIWESGGEVDQETRLFDPARGETRSLRSKEDAHDYRYFPDPDLLPLVIEEAWVESL
HHHEEEECCCCCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAVLPELPDAKRQRFQDEYGIPRYDAGVLVAEQATADFYETVARGRDPRLAANWVIGDLF
HHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AALNRTGRTIQDSPISADALGGMLDLVTDGTINGKIAKEVFEDMLETGDPAAAIVERKGL
HHHHHCCCCCCCCCCCHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
RQVTDTGAIDAAVQAVLAANADKLAEYRSGKDKLFGFFVGQVMKAMAGKGNPALVNEALK
HHHHCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KVL
HHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA