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The map label for this gene is mhpD [H]

Identifier: 162148510

GI number: 162148510

Start: 2826918

End: 2827715

Strand: Direct

Name: mhpD [H]

Synonym: GDI_2732

Alternate gene names: 162148510

Gene position: 2826918-2827715 (Clockwise)

Preceding gene: 162148509

Following gene: 162148511

Centisome position: 71.67

GC content: 56.89

Gene sequence:

>798_bases
ATGAGCACCCCCATGGCACAAACCAACGACATCCAGCGCTGGGCGCGCACGCTTGACGAGGCCACACGCAATCGGAAACC
CATTACTCCTATCAGCATCGAAACTGGCTCCGACAACGTGATGTTCGGCTATCAGGTTCAAACCGCGTGGCGCGAACTGG
CACTCAGCCAAGGTCGGCGCTTGATCGGCCATAAAATCGGCCTGACATCTCCGGAGGTCCAGCGCCAGCTTGGCGTTGAC
GAGCCAGATAGCGGAACGCTGTTCGCGGACATGGAAATACCCGATGAAGGAATGATTCCGCTCGCTCACCTCATCCAGCC
ACGCATCGAAGCAGAAATCGCGTTCGTCCTGGGGCGTGATTTCGTCCACCCCGACCCGACAATGGCGGAATTAATTCGCT
CGATCGATCATGTCCTGCCCGCACTGGAAATTGTCGATAGCCGTATTGAGGATTGGAAAATCCGAGCGATCGATACAATT
GCCGACAATGGATCCTCCGCCCTTTTCATCATGGGAACGCGACCAAAGCTGCTGGGGGACGTCGATCTACAACTTTGCGG
CATGACGATGACCCGTAATGGCCGCATCGTTTCGATCGGTGTCGGCGCCGCATGTCTTGGACATCCGCTCCATGCGCTGC
GCTGGTTCCTACGTCATAAGGCAGTGCTCGGCGAGCCGCTGAAGGCGGGAGAAGTCGTTCTCAGCGGAGCGCTTGGCCCT
ATGATTCCTCTTGAAGGCGATAGCCACTACTCCACGAATATCGCCCACATGGGAACCGTCTCATGCTCTACCGAATAA

Upstream 100 bases:

>100_bases
GCGTCGCGGCGGAACGTGTCACCACGGTGGCGGCATTCAGGGAGACGTTCGGTCGAGCCATTCGAGCGACCGGCCCCATC
TTGATTGAAGCCGTCATCGC

Downstream 100 bases:

>100_bases
GTTCGTTATGGGGGCTCGTCATCGTTCTGGCGGTTAGCTCTACTTCCCAAGCTGCGCTAGCTGCGGATGCCGGATCGAAA
CGTGTGCTCGTTATCCTTTC

Product: 2-keto-4-pentenoate hydratase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSTPMAQTNDIQRWARTLDEATRNRKPITPISIETGSDNVMFGYQVQTAWRELALSQGRRLIGHKIGLTSPEVQRQLGVD
EPDSGTLFADMEIPDEGMIPLAHLIQPRIEAEIAFVLGRDFVHPDPTMAELIRSIDHVLPALEIVDSRIEDWKIRAIDTI
ADNGSSALFIMGTRPKLLGDVDLQLCGMTMTRNGRIVSIGVGAACLGHPLHALRWFLRHKAVLGEPLKAGEVVLSGALGP
MIPLEGDSHYSTNIAHMGTVSCSTE

Sequences:

>Translated_265_residues
MSTPMAQTNDIQRWARTLDEATRNRKPITPISIETGSDNVMFGYQVQTAWRELALSQGRRLIGHKIGLTSPEVQRQLGVD
EPDSGTLFADMEIPDEGMIPLAHLIQPRIEAEIAFVLGRDFVHPDPTMAELIRSIDHVLPALEIVDSRIEDWKIRAIDTI
ADNGSSALFIMGTRPKLLGDVDLQLCGMTMTRNGRIVSIGVGAACLGHPLHALRWFLRHKAVLGEPLKAGEVVLSGALGP
MIPLEGDSHYSTNIAHMGTVSCSTE
>Mature_264_residues
STPMAQTNDIQRWARTLDEATRNRKPITPISIETGSDNVMFGYQVQTAWRELALSQGRRLIGHKIGLTSPEVQRQLGVDE
PDSGTLFADMEIPDEGMIPLAHLIQPRIEAEIAFVLGRDFVHPDPTMAELIRSIDHVLPALEIVDSRIEDWKIRAIDTIA
DNGSSALFIMGTRPKLLGDVDLQLCGMTMTRNGRIVSIGVGAACLGHPLHALRWFLRHKAVLGEPLKAGEVVLSGALGPM
IPLEGDSHYSTNIAHMGTVSCSTE

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=253, Percent_Identity=45.4545454545455, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 29016; Mature: 28885

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPMAQTNDIQRWARTLDEATRNRKPITPISIETGSDNVMFGYQVQTAWRELALSQGRR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHCCH
LIGHKIGLTSPEVQRQLGVDEPDSGTLFADMEIPDEGMIPLAHLIQPRIEAEIAFVLGRD
HHHHHCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCHHHHEEEEEECCC
FVHPDPTMAELIRSIDHVLPALEIVDSRIEDWKIRAIDTIADNGSSALFIMGTRPKLLGD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCCC
VDLQLCGMTMTRNGRIVSIGVGAACLGHPLHALRWFLRHKAVLGEPLKAGEVVLSGALGP
CCEEEECEEEECCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
MIPLEGDSHYSTNIAHMGTVSCSTE
CCCCCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
STPMAQTNDIQRWARTLDEATRNRKPITPISIETGSDNVMFGYQVQTAWRELALSQGRR
CCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHCCH
LIGHKIGLTSPEVQRQLGVDEPDSGTLFADMEIPDEGMIPLAHLIQPRIEAEIAFVLGRD
HHHHHCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCHHHHEEEEEECCC
FVHPDPTMAELIRSIDHVLPALEIVDSRIEDWKIRAIDTIADNGSSALFIMGTRPKLLGD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCCC
VDLQLCGMTMTRNGRIVSIGVGAACLGHPLHALRWFLRHKAVLGEPLKAGEVVLSGALGP
CCEEEECEEEECCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
MIPLEGDSHYSTNIAHMGTVSCSTE
CCCCCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537203 [H]