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The map label for this gene is ilvX [H]

Identifier: 162148509

GI number: 162148509

Start: 2825344

End: 2826921

Strand: Direct

Name: ilvX [H]

Synonym: GDI_2731

Alternate gene names: 162148509

Gene position: 2825344-2826921 (Clockwise)

Preceding gene: 162148508

Following gene: 162148510

Centisome position: 71.63

GC content: 62.93

Gene sequence:

>1578_bases
ATGTCCGTACCACCTGAAAACGGCACTTCGCTCAGCGGTGCCGAAAGCCTTTTGCATACGCTCGTCGCCTGTGGCGTCGA
TACGTGTTTTGCCAATCCCGGCACGTCCGAGATGCACTTCGTGGCTGCCCTCGATGCTGTTCCCGCGATGCGCCCGGTGC
TTTGTCTGTTCGAAGGAGTCGCAACCGGAGCGGCCGACGGATATGCCCGCATGGCGGGGAAACCCGCCTCGACACTGCTG
CATCTCGGCCCGGGCCTCGCGAACGGCCTCGCCGGCTTGCACAACGCACGACGCGCGCAATCGTCGATCGTCAATATCGT
CGGGGACCATGCCACCTACCACCTCCAGTATGACGCACCCCTGACCTCTGACATCGCGGGCTTCGCGCGCCCTGTATCCG
ACTGGGTTCGCCGACCGGCCTCGGCCCGCGCCCTTCCGGGAGATGTCGCCACAGCGGTCGCCTGCGCCCGTCGTTCACCT
GGGCAGATCGCCACGCTGATCTTACCCGCCGATACCGCCTGGGCTCCTGCCGAGCGGATCGGCCTGCCTGAAACCGAGAT
GTTGCCGGCGACGACCCCCGAAAGCACGCTTGAACATGTTGCTGGCCTTTTACGCGGTCCCAGCCGATCGGCAATGCTCC
TGCGTGGCGATGTCCTTCACGGGCAAGGGCTACAGTGGGCAGCGCGTATAGCAGCACGAACAGGAACGAAGTTGTTCTGT
GATACCTTTGCGCCCAGGATCCGCCGGGGAAGGCATCACCCTGTTATCGAGCGACTGCATTACCGGGGTGACGAAGTCCT
ACGGCAACTGGCGGCATTTGATCAGTTGGTCCTCGTCGGGGCGGAACCACCGGTCGCGTTTTTCGCCTATCCTGGCCGAC
CGAGTTGGCTGGTCCCGCCGGACTGCGCGCTGCATACACTCACACTCCCCCGCGAAGACGGGCCGGATGCTCTCGAGCGC
CTGGCGGATCGTCTTGAGGCAAAAACATATTGCCCTCCTGCTCGGCTTACCGTGGCCGCTCCGCAGTCCGATGAATCGCT
GACGCCGGACAGTATCCTCCGCTGCGTCACCATTGACCTTCCGGAAGACGCGATCGTCATCGACGAAGGACTGACATCGA
CCTTTCCCAATTATGATCTTCTGGATGCGGCATTGCCCCACGACCACCTGAACCTGACGGGAGGCGCCATCGGAAGCGGC
ATTCCGCTCAGCGTTGGGGCGGCAGTCGCCGCTCCTACGCGCAAGGTCGTCTGCCTGCACGGCGATGGGGGTGCGATGTA
CAATCTACAAGGACTATGGACTCAGGCACGCGAACGTCTGGACGTCATCACGATCATCTTCGCCAATCGGGCGTACGCAA
TTCTGGAGCAGGAGTTGGACCGCGTCGGAGCACGCGTCGACGGAACGCGATCACGCAGGCTTCTGGATATTGGTGACCCC
GATCTCGATTGGACCGCCCTCGCGACAGGAATGGGCGTCGCGGCGGAACGTGTCACCACGGTGGCGGCATTCAGGGAGAC
GTTCGGTCGAGCCATTCGAGCGACCGGCCCCATCTTGATTGAAGCCGTCATCGCATGA

Upstream 100 bases:

>100_bases
CCTCGTACGTGACCGGCGCGCTCATCCCCGTCGATGGCGGGCTCCTGCACGCTCACTGAACGCTGTTTACCAAATACGCT
TGCTCCCAAGGACCACAATC

Downstream 100 bases:

>100_bases
GCACCCCCATGGCACAAACCAACGACATCCAGCGCTGGGCGCGCACGCTTGACGAGGCCACACGCAATCGGAAACCCATT
ACTCCTATCAGCATCGAAAC

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSVPPENGTSLSGAESLLHTLVACGVDTCFANPGTSEMHFVAALDAVPAMRPVLCLFEGVATGAADGYARMAGKPASTLL
HLGPGLANGLAGLHNARRAQSSIVNIVGDHATYHLQYDAPLTSDIAGFARPVSDWVRRPASARALPGDVATAVACARRSP
GQIATLILPADTAWAPAERIGLPETEMLPATTPESTLEHVAGLLRGPSRSAMLLRGDVLHGQGLQWAARIAARTGTKLFC
DTFAPRIRRGRHHPVIERLHYRGDEVLRQLAAFDQLVLVGAEPPVAFFAYPGRPSWLVPPDCALHTLTLPREDGPDALER
LADRLEAKTYCPPARLTVAAPQSDESLTPDSILRCVTIDLPEDAIVIDEGLTSTFPNYDLLDAALPHDHLNLTGGAIGSG
IPLSVGAAVAAPTRKVVCLHGDGGAMYNLQGLWTQARERLDVITIIFANRAYAILEQELDRVGARVDGTRSRRLLDIGDP
DLDWTALATGMGVAAERVTTVAAFRETFGRAIRATGPILIEAVIA

Sequences:

>Translated_525_residues
MSVPPENGTSLSGAESLLHTLVACGVDTCFANPGTSEMHFVAALDAVPAMRPVLCLFEGVATGAADGYARMAGKPASTLL
HLGPGLANGLAGLHNARRAQSSIVNIVGDHATYHLQYDAPLTSDIAGFARPVSDWVRRPASARALPGDVATAVACARRSP
GQIATLILPADTAWAPAERIGLPETEMLPATTPESTLEHVAGLLRGPSRSAMLLRGDVLHGQGLQWAARIAARTGTKLFC
DTFAPRIRRGRHHPVIERLHYRGDEVLRQLAAFDQLVLVGAEPPVAFFAYPGRPSWLVPPDCALHTLTLPREDGPDALER
LADRLEAKTYCPPARLTVAAPQSDESLTPDSILRCVTIDLPEDAIVIDEGLTSTFPNYDLLDAALPHDHLNLTGGAIGSG
IPLSVGAAVAAPTRKVVCLHGDGGAMYNLQGLWTQARERLDVITIIFANRAYAILEQELDRVGARVDGTRSRRLLDIGDP
DLDWTALATGMGVAAERVTTVAAFRETFGRAIRATGPILIEAVIA
>Mature_524_residues
SVPPENGTSLSGAESLLHTLVACGVDTCFANPGTSEMHFVAALDAVPAMRPVLCLFEGVATGAADGYARMAGKPASTLLH
LGPGLANGLAGLHNARRAQSSIVNIVGDHATYHLQYDAPLTSDIAGFARPVSDWVRRPASARALPGDVATAVACARRSPG
QIATLILPADTAWAPAERIGLPETEMLPATTPESTLEHVAGLLRGPSRSAMLLRGDVLHGQGLQWAARIAARTGTKLFCD
TFAPRIRRGRHHPVIERLHYRGDEVLRQLAAFDQLVLVGAEPPVAFFAYPGRPSWLVPPDCALHTLTLPREDGPDALERL
ADRLEAKTYCPPARLTVAAPQSDESLTPDSILRCVTIDLPEDAIVIDEGLTSTFPNYDLLDAALPHDHLNLTGGAIGSGI
PLSVGAAVAAPTRKVVCLHGDGGAMYNLQGLWTQARERLDVITIIFANRAYAILEQELDRVGARVDGTRSRRLLDIGDPD
LDWTALATGMGVAAERVTTVAAFRETFGRAIRATGPILIEAVIA

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=122, Percent_Identity=35.2459016393443, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 55731; Mature: 55600

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPPENGTSLSGAESLLHTLVACGVDTCFANPGTSEMHFVAALDAVPAMRPVLCLFEGV
CCCCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
ATGAADGYARMAGKPASTLLHLGPGLANGLAGLHNARRAQSSIVNIVGDHATYHLQYDAP
HCCCCCCHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
LTSDIAGFARPVSDWVRRPASARALPGDVATAVACARRSPGQIATLILPADTAWAPAERI
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHC
GLPETEMLPATTPESTLEHVAGLLRGPSRSAMLLRGDVLHGQGLQWAARIAARTGTKLFC
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCEECCCCHHHHHHHHHHCCCCHHH
DTFAPRIRRGRHHPVIERLHYRGDEVLRQLAAFDQLVLVGAEPPVAFFAYPGRPSWLVPP
HHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCC
DCALHTLTLPREDGPDALERLADRLEAKTYCPPARLTVAAPQSDESLTPDSILRCVTIDL
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHEEEEEEEEC
PEDAIVIDEGLTSTFPNYDLLDAALPHDHLNLTGGAIGSGIPLSVGAAVAAPTRKVVCLH
CCCCEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEEE
GDGGAMYNLQGLWTQARERLDVITIIFANRAYAILEQELDRVGARVDGTRSRRLLDIGDP
CCCCCEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
DLDWTALATGMGVAAERVTTVAAFRETFGRAIRATGPILIEAVIA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
SVPPENGTSLSGAESLLHTLVACGVDTCFANPGTSEMHFVAALDAVPAMRPVLCLFEGV
CCCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
ATGAADGYARMAGKPASTLLHLGPGLANGLAGLHNARRAQSSIVNIVGDHATYHLQYDAP
HCCCCCCHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
LTSDIAGFARPVSDWVRRPASARALPGDVATAVACARRSPGQIATLILPADTAWAPAERI
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHC
GLPETEMLPATTPESTLEHVAGLLRGPSRSAMLLRGDVLHGQGLQWAARIAARTGTKLFC
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCEECCCCHHHHHHHHHHCCCCHHH
DTFAPRIRRGRHHPVIERLHYRGDEVLRQLAAFDQLVLVGAEPPVAFFAYPGRPSWLVPP
HHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCC
DCALHTLTLPREDGPDALERLADRLEAKTYCPPARLTVAAPQSDESLTPDSILRCVTIDL
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHEEEEEEEEC
PEDAIVIDEGLTSTFPNYDLLDAALPHDHLNLTGGAIGSGIPLSVGAAVAAPTRKVVCLH
CCCCEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEEE
GDGGAMYNLQGLWTQARERLDVITIIFANRAYAILEQELDRVGARVDGTRSRRLLDIGDP
CCCCCEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
DLDWTALATGMGVAAERVTTVAAFRETFGRAIRATGPILIEAVIA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]