BacMap

Definition	Gluconacetobacter diazotrophicus PAl 5 chromosome, complete genome.
Accession	NC_010125
Length	3,944,163

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The map label for this gene is nodG [H]

Identifier: 162148508

GI number: 162148508

Start: 2824475

End: 2825302

Strand: Direct

Name: nodG [H]

Synonym: GDI_2730

Alternate gene names: 162148508

Gene position: 2824475-2825302 (Clockwise)

Preceding gene: 162148507

Following gene: 162148509

Centisome position: 71.61

GC content: 62.92

Gene sequence:

>828_bases
ATGCCATGTCGCCTGGTTCAGGGAAAAAAGGGACACGGAATGGATCTTCAAATATCGGGCCGAACGGCCATTGTCATGGG
TGCCAGCAGCGGCCTTGGCCTCGGAATTGCCCATGCCCTTGCAAGGGCAGGCGTGCATGTCGGCCTGCTCTCCCGCGACC
CGTCACGCCTGGAAGCGGCGGCGGCAAGTCTCCGTGCTGTTTCGTCGGCAACAATATCATGCATCGTGGCCGATCTGGCA
TCGCAGGACGCAGTTCATGCGGCGATCAGCACGATAAAAGATGAGGTCGGTGTTCCTGACATTCTGATCGGCAATGGTGG
TGGCCCGCCGCCCAGTTCCGCCACCGTCTTCGATGCCGCGCTCTGGGAAGCCCAGGCGCGGGCGATGCTGTTGCCCGTGA
TGACCATGACCGACGCGTTCCTGCCGAACATGCGCGCAAACGGTTTCGGACGCCTCATCATGGTCTCGTCCACCAGCGTC
GTCGAACCGATCCCGGGTCTTGTCCTGTCCAATGCCCTGAGAGCAGCGCTCGCCAATTGGGGGCGCACTCTGGCGCGTGA
AGTCGCGCGTGACGGCGTCACCGTCAATACGTTGATGCCTGGCCGCTTCGATACCGCTCGGGTCCAAAAACTCAACCAGT
CACGCGCGAACACCGAAGGTGGCTCCGCCGAGGACGTTCGGCGACAGGATCTCGAAACCATACCAGCAGGCCGCTATGGG
ACACCCGAGGAATTCGGCGCCATCGCCGCCTTCCTCGCCAGCCCTTTGGCCTCGTACGTGACCGGCGCGCTCATCCCCGT
CGATGGCGGGCTCCTGCACGCTCACTGA

Upstream 100 bases:

>100_bases
CGTGATGACCTGAAGTCATCAACCCAGGCAAACGAGCATTGGATCCTGTGGTCCATCAGCGCGTAATTTTCCTGTCCAGA
AAACCTGAAAAGCGGCGGCG

Downstream 100 bases:

>100_bases
ACGCTGTTTACCAAATACGCTTGCTCCCAAGGACCACAATCATGTCCGTACCACCTGAAAACGGCACTTCGCTCAGCGGT
GCCGAAAGCCTTTTGCATAC

Product: oxidoreductase protein

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPCRLVQGKKGHGMDLQISGRTAIVMGASSGLGLGIAHALARAGVHVGLLSRDPSRLEAAAASLRAVSSATISCIVADLA
SQDAVHAAISTIKDEVGVPDILIGNGGGPPPSSATVFDAALWEAQARAMLLPVMTMTDAFLPNMRANGFGRLIMVSSTSV
VEPIPGLVLSNALRAALANWGRTLAREVARDGVTVNTLMPGRFDTARVQKLNQSRANTEGGSAEDVRRQDLETIPAGRYG
TPEEFGAIAAFLASPLASYVTGALIPVDGGLLHAH

Sequences:

>Translated_275_residues
MPCRLVQGKKGHGMDLQISGRTAIVMGASSGLGLGIAHALARAGVHVGLLSRDPSRLEAAAASLRAVSSATISCIVADLA
SQDAVHAAISTIKDEVGVPDILIGNGGGPPPSSATVFDAALWEAQARAMLLPVMTMTDAFLPNMRANGFGRLIMVSSTSV
VEPIPGLVLSNALRAALANWGRTLAREVARDGVTVNTLMPGRFDTARVQKLNQSRANTEGGSAEDVRRQDLETIPAGRYG
TPEEFGAIAAFLASPLASYVTGALIPVDGGLLHAH
>Mature_274_residues
PCRLVQGKKGHGMDLQISGRTAIVMGASSGLGLGIAHALARAGVHVGLLSRDPSRLEAAAASLRAVSSATISCIVADLAS
QDAVHAAISTIKDEVGVPDILIGNGGGPPPSSATVFDAALWEAQARAMLLPVMTMTDAFLPNMRANGFGRLIMVSSTSVV
EPIPGLVLSNALRAALANWGRTLAREVARDGVTVNTLMPGRFDTARVQKLNQSRANTEGGSAEDVRRQDLETIPAGRYGT
PEEFGAIAAFLASPLASYVTGALIPVDGGLLHAH

Specific function: Proposed to modify Nod factor fatty acyl chain [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=29.5454545454545, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI32483357, Length=255, Percent_Identity=28.6274509803922, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI5031737, Length=276, Percent_Identity=30.0724637681159, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI66933014, Length=183, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI126723750, Length=262, Percent_Identity=25.5725190839695, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI226371731, Length=186, Percent_Identity=31.1827956989247, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI33667109, Length=278, Percent_Identity=26.978417266187, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI40254992, Length=252, Percent_Identity=26.5873015873016, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4557649, Length=221, Percent_Identity=26.2443438914027, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4503301, Length=254, Percent_Identity=27.1653543307087, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=32.9411764705882, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=28.515625, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789208, Length=260, Percent_Identity=31.9230769230769, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082160, Length=270, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790717, Length=259, Percent_Identity=25.0965250965251, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787905, Length=261, Percent_Identity=25.2873563218391, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789378, Length=279, Percent_Identity=27.2401433691756, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI2367365, Length=265, Percent_Identity=28.6792452830189, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788459, Length=264, Percent_Identity=25.3787878787879, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17560676, Length=259, Percent_Identity=27.027027027027, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=27.3764258555133, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=258, Percent_Identity=26.7441860465116, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=28.4644194756554, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=270, Percent_Identity=27.4074074074074, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=269, Percent_Identity=28.2527881040892, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=255, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=29.5454545454545, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=28.735632183908, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=268, Percent_Identity=28.7313432835821, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=274, Percent_Identity=27.3722627737226, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=263, Percent_Identity=26.615969581749, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=254, Percent_Identity=29.5275590551181, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=275, Percent_Identity=26.5454545454545, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=26.2357414448669, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=270, Percent_Identity=27.4074074074074, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17561272, Length=268, Percent_Identity=26.1194029850746, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=270, Percent_Identity=24.8148148148148, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17536651, Length=261, Percent_Identity=25.2873563218391, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=275, Percent_Identity=26.5454545454545, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=270, Percent_Identity=25.5555555555556, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=262, Percent_Identity=23.6641221374046, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=32.156862745098, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=26.8482490272374, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=29.5275590551181, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21355319, Length=258, Percent_Identity=26.3565891472868, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571526, Length=259, Percent_Identity=29.7297297297297, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI161076933, Length=185, Percent_Identity=30.8108108108108, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24584748, Length=185, Percent_Identity=30.8108108108108, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24644337, Length=125, Percent_Identity=39.2, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584744, Length=186, Percent_Identity=28.494623655914, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28574402, Length=186, Percent_Identity=28.494623655914, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640442, Length=228, Percent_Identity=27.6315789473684, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358495, Length=197, Percent_Identity=28.4263959390863, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011283
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 28376; Mature: 28245

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCRLVQGKKGHGMDLQISGRTAIVMGASSGLGLGIAHALARAGVHVGLLSRDPSRLEAA
CCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHH
AASLRAVSSATISCIVADLASQDAVHAAISTIKDEVGVPDILIGNGGGPPPSSATVFDAA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHH
LWEAQARAMLLPVMTMTDAFLPNMRANGFGRLIMVSSTSVVEPIPGLVLSNALRAALANW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHH
GRTLAREVARDGVTVNTLMPGRFDTARVQKLNQSRANTEGGSAEDVRRQDLETIPAGRYG
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
TPEEFGAIAAFLASPLASYVTGALIPVDGGLLHAH
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECC
>Mature Secondary Structure 
PCRLVQGKKGHGMDLQISGRTAIVMGASSGLGLGIAHALARAGVHVGLLSRDPSRLEAA
CCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHH
AASLRAVSSATISCIVADLASQDAVHAAISTIKDEVGVPDILIGNGGGPPPSSATVFDAA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHH
LWEAQARAMLLPVMTMTDAFLPNMRANGFGRLIMVSSTSVVEPIPGLVLSNALRAALANW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHH
GRTLAREVARDGVTVNTLMPGRFDTARVQKLNQSRANTEGGSAEDVRRQDLETIPAGRYG
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
TPEEFGAIAAFLASPLASYVTGALIPVDGGLLHAH
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1538694; 2243795 [H]