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The map label for this gene is gcvA [H]

Identifier: 162148507

GI number: 162148507

Start: 2823476

End: 2824366

Strand: Direct

Name: gcvA [H]

Synonym: GDI_2729

Alternate gene names: 162148507

Gene position: 2823476-2824366 (Clockwise)

Preceding gene: 162148506

Following gene: 162148508

Centisome position: 71.59

GC content: 61.17

Gene sequence:

>891_bases
ATGCGTCGACGTCTGCCCCTGACGGCTATGGAAGCGTTCTGCGCGGTCGCGCGGCATCTCAGCTTTCGCGGCGCCGCTGG
GTCTATTGGCATTTCTCAAAGCGCGCTCACCCGTCATGTCCAAAACCTTGAACGGGATCTGGGCGTACCGCTTTTCGAGA
GACGTGGCAACACCATTAAGCTGACGGAAAAAGGCCGTCTGCTCTTCGAGAAGGCAGAACAGGCCCTCCAATGGCTTCAC
GACGGTCTGTCTCAGGTCACCGAGCGCGCGGCTTCCCCGCTGCGTGTGAGCGTTCTACCCTCCCTGGCCGCGTCATGGCT
TGCCCCTCGTCTGCCTGCCTTCACGACGAAGCATCGGGATATCACTGTCGATCTCGTGCCGGACTACCACCTCGCCGACC
TTAATGCCGGATCGACGGACCTGGCGTTGCGTTACGGAATAGGTCCATGGCGGGGAACCTGGTCTCGCATGTTCATGCGC
GAGGAACTGGCTCCCGTCGCCGCTCCGACCGCTTGGCAAGACAGCACGGACGTGGAGCAGATATTCGCCGGCAGGACATT
ATTCGGCGCGGCGCAACCTTTCGAATGGGATCGACTGCTCGACGGGCTTGGCCTCTCCTCCAAGCCCGCTGTCACGTCGC
TGAATGACTATAATGTGGTCATCAGCGCGACCTTGGCGGGCCAGGGCATCATGGTCGGCAGACGCTCCCTGATCCAGTCT
CATTTCGACGATGGACGTCTGGTGCGTGTTGGTGCGCCCTGGATGGATTGCGGAACCGCCTATCATGTCGTCACCCGACC
CGGACGCCAGCATGAACGCGCGATCAGCGCTTTCATTGCGTGGCTAACGTCCGAAGGAGCCGCCACAAACGAACTCTGGG
CGCCTCATTGA

Upstream 100 bases:

>100_bases
CGCCCGCAAAAGTTCGGGCGTTTCTGGATTTCATTTGTAATGATGTCGCGACCGTCCAAATAAAGCACGATGGATTTATC
GGTCTGTAAGGGAGGACATT

Downstream 100 bases:

>100_bases
TTGCCTCACGTGATGACCTGAAGTCATCAACCCAGGCAAACGAGCATTGGATCCTGTGGTCCATCAGCGCGTAATTTTCC
TGTCCAGAAAACCTGAAAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRRRLPLTAMEAFCAVARHLSFRGAAGSIGISQSALTRHVQNLERDLGVPLFERRGNTIKLTEKGRLLFEKAEQALQWLH
DGLSQVTERAASPLRVSVLPSLAASWLAPRLPAFTTKHRDITVDLVPDYHLADLNAGSTDLALRYGIGPWRGTWSRMFMR
EELAPVAAPTAWQDSTDVEQIFAGRTLFGAAQPFEWDRLLDGLGLSSKPAVTSLNDYNVVISATLAGQGIMVGRRSLIQS
HFDDGRLVRVGAPWMDCGTAYHVVTRPGRQHERAISAFIAWLTSEGAATNELWAPH

Sequences:

>Translated_296_residues
MRRRLPLTAMEAFCAVARHLSFRGAAGSIGISQSALTRHVQNLERDLGVPLFERRGNTIKLTEKGRLLFEKAEQALQWLH
DGLSQVTERAASPLRVSVLPSLAASWLAPRLPAFTTKHRDITVDLVPDYHLADLNAGSTDLALRYGIGPWRGTWSRMFMR
EELAPVAAPTAWQDSTDVEQIFAGRTLFGAAQPFEWDRLLDGLGLSSKPAVTSLNDYNVVISATLAGQGIMVGRRSLIQS
HFDDGRLVRVGAPWMDCGTAYHVVTRPGRQHERAISAFIAWLTSEGAATNELWAPH
>Mature_296_residues
MRRRLPLTAMEAFCAVARHLSFRGAAGSIGISQSALTRHVQNLERDLGVPLFERRGNTIKLTEKGRLLFEKAEQALQWLH
DGLSQVTERAASPLRVSVLPSLAASWLAPRLPAFTTKHRDITVDLVPDYHLADLNAGSTDLALRYGIGPWRGTWSRMFMR
EELAPVAAPTAWQDSTDVEQIFAGRTLFGAAQPFEWDRLLDGLGLSSKPAVTSLNDYNVVISATLAGQGIMVGRRSLIQS
HFDDGRLVRVGAPWMDCGTAYHVVTRPGRQHERAISAFIAWLTSEGAATNELWAPH

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=31, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788706, Length=310, Percent_Identity=27.4193548387097, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=27.6450511945392, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693193, Length=285, Percent_Identity=25.9649122807018, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=25.0847457627119, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=37.9310344827586, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786401, Length=137, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=311, Percent_Identity=25.08038585209, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=269, Percent_Identity=25.6505576208178, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32660; Mature: 32660

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRLPLTAMEAFCAVARHLSFRGAAGSIGISQSALTRHVQNLERDLGVPLFERRGNTIK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCEEE
LTEKGRLLFEKAEQALQWLHDGLSQVTERAASPLRVSVLPSLAASWLAPRLPAFTTKHRD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCE
ITVDLVPDYHLADLNAGSTDLALRYGIGPWRGTWSRMFMREELAPVAAPTAWQDSTDVEQ
EEEEECCCCEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
IFAGRTLFGAAQPFEWDRLLDGLGLSSKPAVTSLNDYNVVISATLAGQGIMVGRRSLIQS
HHCCCHHCCCCCCCCHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCHHHHHHHHHHH
HFDDGRLVRVGAPWMDCGTAYHVVTRPGRQHERAISAFIAWLTSEGAATNELWAPH
HCCCCCEEEECCCCHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRRRLPLTAMEAFCAVARHLSFRGAAGSIGISQSALTRHVQNLERDLGVPLFERRGNTIK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCEEE
LTEKGRLLFEKAEQALQWLHDGLSQVTERAASPLRVSVLPSLAASWLAPRLPAFTTKHRD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCE
ITVDLVPDYHLADLNAGSTDLALRYGIGPWRGTWSRMFMREELAPVAAPTAWQDSTDVEQ
EEEEECCCCEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
IFAGRTLFGAAQPFEWDRLLDGLGLSSKPAVTSLNDYNVVISATLAGQGIMVGRRSLIQS
HHCCCHHCCCCCCCCHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCHHHHHHHHHHH
HFDDGRLVRVGAPWMDCGTAYHVVTRPGRQHERAISAFIAWLTSEGAATNELWAPH
HCCCCCEEEECCCCHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]