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The map label for this gene is artJ [H]

Identifier: 162148490

GI number: 162148490

Start: 2808178

End: 2809095

Strand: Reverse

Name: artJ [H]

Synonym: GDI_2711

Alternate gene names: 162148490

Gene position: 2809095-2808178 (Counterclockwise)

Preceding gene: 162148497

Following gene: 162148489

Centisome position: 71.22

GC content: 69.17

Gene sequence:

>918_bases
ATGGAGGAAGCCGGAGTCTTGAGAGCCTGTCTGAAAATGCGCAGTGTCGGCCGACCGGGGCGCGTTTTCCGCATGTCGCG
GCCAAAGGCTGCCGGGGTGCGTTCCGGCGCGCGGAAAGTGGGCGGGATTCTGGCCGGCTGCGTCCTGTCGCTCTTGCCGC
TGTGCATCGGCCAGGGCGCCCAGGCGCGGGCGCTGGCCGATATCCGCGCCAGCGGTCATCTGAATATCGGTGTCAACCCG
GCGCTTCCGCCGCTGGCGGCATATGATTCGCGCAACGTGATCGAGGGATTCGACGTGGATGTCGGAACCGCGCTGGCGGC
GCGGCTGGGGGTGTCGCCCCGTTTGGTTCCGGTATCGTCGAGCGGGCGCATTCCCTACCTGATGGCGGGCAAGGTGGACG
TGGTGCTGGGTGGGCTGACCCGCACGCCTGAACGCGCGGCCGCCATTGCGTTCTCGGCGCCGCTCTATGCCGAGGAATTC
GCCATCATCGCCCCGGCGGTATCCAGGGTGGGGGGGCTGGACGACCTGGCGTCCGGACGGGTGCGGATGGTCGAGGTCCG
GGGTTCCACGGCGGTGCCGTTCCTGCGGACGCATGCGCCGAAGGCACCGATCCTTCTGCTGGGCAGCTATCCGGATGCCG
TGCGCGCCCTGGCCCAGGGGCGGGGTGACGCCATGGTGGACGTCATCGAATATGTCGGCCGGTTTCTGGCGGCCGAACCC
CAGGCCGGATGGACGATCCAGCGCGGCTTCGCCCCCGCCGCGGGCGAGGATTGCGTGGGCGTCGCCCAGTCCGACACCGA
TCTTCTGCGCGTGGTCGACGGGACGCTGGCCGACCTGAAGCGGGATGGAACAATCCAGGCCGCCTATCGTCGCTGGTTCG
GCGTGTCCATGGCCACCCCGGCGGAGGGACGTCCATGA

Upstream 100 bases:

>100_bases
AATCGGTTCGGGCAAGAAACTCTTCCTTGAACGGTGTTTTAATCGTATACAAAGCGTATATGTTCTTTAGAACAGTGATG
CCTGGATCGCAACATCCAAT

Downstream 100 bases:

>100_bases
CGGCGCTGCATTTCCAGCCGGTCCTGCAACGCCTGCCGTTCCTGCTGGGGGGCGCCGCGACGACGGTTCTTCTGTCCGTC
GCGTCCTTTGGTGCCGGCCT

Product: arginine-binding periplasmic protein 2

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEEAGVLRACLKMRSVGRPGRVFRMSRPKAAGVRSGARKVGGILAGCVLSLLPLCIGQGAQARALADIRASGHLNIGVNP
ALPPLAAYDSRNVIEGFDVDVGTALAARLGVSPRLVPVSSSGRIPYLMAGKVDVVLGGLTRTPERAAAIAFSAPLYAEEF
AIIAPAVSRVGGLDDLASGRVRMVEVRGSTAVPFLRTHAPKAPILLLGSYPDAVRALAQGRGDAMVDVIEYVGRFLAAEP
QAGWTIQRGFAPAAGEDCVGVAQSDTDLLRVVDGTLADLKRDGTIQAAYRRWFGVSMATPAEGRP

Sequences:

>Translated_305_residues
MEEAGVLRACLKMRSVGRPGRVFRMSRPKAAGVRSGARKVGGILAGCVLSLLPLCIGQGAQARALADIRASGHLNIGVNP
ALPPLAAYDSRNVIEGFDVDVGTALAARLGVSPRLVPVSSSGRIPYLMAGKVDVVLGGLTRTPERAAAIAFSAPLYAEEF
AIIAPAVSRVGGLDDLASGRVRMVEVRGSTAVPFLRTHAPKAPILLLGSYPDAVRALAQGRGDAMVDVIEYVGRFLAAEP
QAGWTIQRGFAPAAGEDCVGVAQSDTDLLRVVDGTLADLKRDGTIQAAYRRWFGVSMATPAEGRP
>Mature_305_residues
MEEAGVLRACLKMRSVGRPGRVFRMSRPKAAGVRSGARKVGGILAGCVLSLLPLCIGQGAQARALADIRASGHLNIGVNP
ALPPLAAYDSRNVIEGFDVDVGTALAARLGVSPRLVPVSSSGRIPYLMAGKVDVVLGGLTRTPERAAAIAFSAPLYAEEF
AIIAPAVSRVGGLDDLASGRVRMVEVRGSTAVPFLRTHAPKAPILLLGSYPDAVRALAQGRGDAMVDVIEYVGRFLAAEP
QAGWTIQRGFAPAAGEDCVGVAQSDTDLLRVVDGTLADLKRDGTIQAAYRRWFGVSMATPAEGRP

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=226, Percent_Identity=27.8761061946903, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=25.764192139738, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=23.574144486692, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787031, Length=261, Percent_Identity=27.2030651340996, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=25.974025974026, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786876, Length=244, Percent_Identity=22.9508196721311, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31818; Mature: 31818

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEAGVLRACLKMRSVGRPGRVFRMSRPKAAGVRSGARKVGGILAGCVLSLLPLCIGQGA
CCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QARALADIRASGHLNIGVNPALPPLAAYDSRNVIEGFDVDVGTALAARLGVSPRLVPVSS
CHHHHHHHHCCCCEEECCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECC
SGRIPYLMAGKVDVVLGGLTRTPERAAAIAFSAPLYAEEFAIIAPAVSRVGGLDDLASGR
CCCCCEEECCCEEEEECCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHCCCHHHCCCC
VRMVEVRGSTAVPFLRTHAPKAPILLLGSYPDAVRALAQGRGDAMVDVIEYVGRFLAAEP
EEEEEECCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
QAGWTIQRGFAPAAGEDCVGVAQSDTDLLRVVDGTLADLKRDGTIQAAYRRWFGVSMATP
CCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
AEGRP
CCCCC
>Mature Secondary Structure
MEEAGVLRACLKMRSVGRPGRVFRMSRPKAAGVRSGARKVGGILAGCVLSLLPLCIGQGA
CCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QARALADIRASGHLNIGVNPALPPLAAYDSRNVIEGFDVDVGTALAARLGVSPRLVPVSS
CHHHHHHHHCCCCEEECCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECC
SGRIPYLMAGKVDVVLGGLTRTPERAAAIAFSAPLYAEEFAIIAPAVSRVGGLDDLASGR
CCCCCEEECCCEEEEECCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHCCCHHHCCCC
VRMVEVRGSTAVPFLRTHAPKAPILLLGSYPDAVRALAQGRGDAMVDVIEYVGRFLAAEP
EEEEEECCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
QAGWTIQRGFAPAAGEDCVGVAQSDTDLLRVVDGTLADLKRDGTIQAAYRRWFGVSMATP
CCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
AEGRP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]