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The map label for this gene is xylF [H]

Identifier: 162148317

GI number: 162148317

Start: 2630267

End: 2631319

Strand: Reverse

Name: xylF [H]

Synonym: GDI_2534

Alternate gene names: 162148317

Gene position: 2631319-2630267 (Counterclockwise)

Preceding gene: 162148318

Following gene: 162148316

Centisome position: 66.71

GC content: 59.54

Gene sequence:

>1053_bases
TTGGTGAACAATAACAACAAGATTATCAAATGCCGCGCGTCTGTCACGCGTCGGGCAGCCCTTGGCGTGCTCATGGCCAG
CGCGTTGTGCGCGATGGTGCCCCATGGGGCCATCGCCAGCGCCGCCCATCCGAAAATCGGTTTTTCCATCGACGACCTTC
GCGTGGAACGCTGGACGCATGACCGCGACTATTTTGTCGCTGCTGCGACGAAACTGGGCGCGACCGTCAACGTGCAGTCG
GCGAATGCCAGCGAATCCCGCCAGATGGCGCAGATTGAAAACCTGATATCCCGCGGGATGGATGTTATCGTCATCGTGCC
GTTCAACGGCAAGGTGCTGGCCAATACGATTGCCGAAGCAAAGCAGGCCGGAATCAAGGTGATTTCCTATGATCGCCTGA
TCCTGGATTCGGATATCGACGCCTATATCTCGTTCGACAATGTGCGCGTGGGTGAAATGCAGGCCCAGGGCGTCGTGGAT
GCGGCACCGACCGGAAATTATTATCTGCTGGGCGGCGCGCCGACCGACAACAACGCGAAGTTGCTGCGCGAAGGCCAGAT
GAAGGTGCTGCAGCCCCTGGTGGACAGCCACAAGATCAAGATCGTGGGCGCCCAGTGGGTCAAGGAATGGAGCCCGACCG
AGGCGCTTGCCATCACCGAAAACGCCCTGACCGCCACCGCCAACAAGATCGACGGCATCGTCGCGTCCAATGACACGATC
GCGGGGGGTGCGATCCAGGCGCTGGCCGCGCAGGGCCTGGCAGGCAAGACGGCCATTTCCGGGCAGGATGCCGACCTTGC
GGCGTTGCAGCGCATCAACAAGGGCACGCAGACCATGACGGTCTACAAGCCGTTGAAGGATATCGCATCCACCGCAGCGC
ACCTGGCGGTCGACCTGGCAAAGGGGACGACGCCCACTTATACGACCAAGATGAACAATGGGCAGAAGGATGTGAATACC
GTTCTGCTGACGCCGATTTCACTGACCAAGGCCAATCTCGATCGCGTCGTGAAGGACGGGTATTTCACGATGGCACAGAT
CGAAGGACAGTAA

Upstream 100 bases:

>100_bases
TTTAGATAACGCCAGCAAGCCAAAAAGGCATTGCAAAATGACTGGCGAGGGATCGAACGACGCCTGATGGCGAAATCTGT
TCGGCAAGAGCGGAGTGTGT

Downstream 100 bases:

>100_bases
TCCTTCGCCGTGTGCGCGGGAAGACGGATACTCCTTCCCGCGCATCCCGATTGGAATGGACACGCGATACGCGAGGACAC
GGGATATGGCCGAATACCTT

Product: D-xylose-binding periplasmic protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MVNNNNKIIKCRASVTRRAALGVLMASALCAMVPHGAIASAAHPKIGFSIDDLRVERWTHDRDYFVAAATKLGATVNVQS
ANASESRQMAQIENLISRGMDVIVIVPFNGKVLANTIAEAKQAGIKVISYDRLILDSDIDAYISFDNVRVGEMQAQGVVD
AAPTGNYYLLGGAPTDNNAKLLREGQMKVLQPLVDSHKIKIVGAQWVKEWSPTEALAITENALTATANKIDGIVASNDTI
AGGAIQALAAQGLAGKTAISGQDADLAALQRINKGTQTMTVYKPLKDIASTAAHLAVDLAKGTTPTYTTKMNNGQKDVNT
VLLTPISLTKANLDRVVKDGYFTMAQIEGQ

Sequences:

>Translated_350_residues
MVNNNNKIIKCRASVTRRAALGVLMASALCAMVPHGAIASAAHPKIGFSIDDLRVERWTHDRDYFVAAATKLGATVNVQS
ANASESRQMAQIENLISRGMDVIVIVPFNGKVLANTIAEAKQAGIKVISYDRLILDSDIDAYISFDNVRVGEMQAQGVVD
AAPTGNYYLLGGAPTDNNAKLLREGQMKVLQPLVDSHKIKIVGAQWVKEWSPTEALAITENALTATANKIDGIVASNDTI
AGGAIQALAAQGLAGKTAISGQDADLAALQRINKGTQTMTVYKPLKDIASTAAHLAVDLAKGTTPTYTTKMNNGQKDVNT
VLLTPISLTKANLDRVVKDGYFTMAQIEGQ
>Mature_350_residues
MVNNNNKIIKCRASVTRRAALGVLMASALCAMVPHGAIASAAHPKIGFSIDDLRVERWTHDRDYFVAAATKLGATVNVQS
ANASESRQMAQIENLISRGMDVIVIVPFNGKVLANTIAEAKQAGIKVISYDRLILDSDIDAYISFDNVRVGEMQAQGVVD
AAPTGNYYLLGGAPTDNNAKLLREGQMKVLQPLVDSHKIKIVGAQWVKEWSPTEALAITENALTATANKIDGIVASNDTI
AGGAIQALAAQGLAGKTAISGQDADLAALQRINKGTQTMTVYKPLKDIASTAAHLAVDLAKGTTPTYTTKMNNGQKDVNT
VLLTPISLTKANLDRVVKDGYFTMAQIEGQ

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=310, Percent_Identity=62.5806451612903, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1790526, Length=256, Percent_Identity=27.734375, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790192, Length=228, Percent_Identity=26.7543859649123, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37201; Mature: 37201

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNNNNKIIKCRASVTRRAALGVLMASALCAMVPHGAIASAAHPKIGFSIDDLRVERWTH
CCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEHHEEEEEECC
DRDYFVAAATKLGATVNVQSANASESRQMAQIENLISRGMDVIVIVPFNGKVLANTIAEA
CCCEEEEEEHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHH
KQAGIKVISYDRLILDSDIDAYISFDNVRVGEMQAQGVVDAAPTGNYYLLGGAPTDNNAK
HHCCEEEEEECEEEEECCCCEEEEECCEEECCEECCCCEECCCCCCEEEEECCCCCCCHH
LLREGQMKVLQPLVDSHKIKIVGAQWVKEWSPTEALAITENALTATANKIDGIVASNDTI
HHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCEEEEECCHHHHHHHHHCCEEECCCCC
AGGAIQALAAQGLAGKTAISGQDADLAALQRINKGTQTMTVYKPLKDIASTAAHLAVDLA
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHEEECC
KGTTPTYTTKMNNGQKDVNTVLLTPISLTKANLDRVVKDGYFTMAQIEGQ
CCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MVNNNNKIIKCRASVTRRAALGVLMASALCAMVPHGAIASAAHPKIGFSIDDLRVERWTH
CCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEHHEEEEEECC
DRDYFVAAATKLGATVNVQSANASESRQMAQIENLISRGMDVIVIVPFNGKVLANTIAEA
CCCEEEEEEHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHH
KQAGIKVISYDRLILDSDIDAYISFDNVRVGEMQAQGVVDAAPTGNYYLLGGAPTDNNAK
HHCCEEEEEECEEEEECCCCEEEEECCEEECCEECCCCEECCCCCCEEEEECCCCCCCHH
LLREGQMKVLQPLVDSHKIKIVGAQWVKEWSPTEALAITENALTATANKIDGIVASNDTI
HHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCEEEEECCHHHHHHHHHCCEEECCCCC
AGGAIQALAAQGLAGKTAISGQDADLAALQRINKGTQTMTVYKPLKDIASTAAHLAVDLA
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHEEECC
KGTTPTYTTKMNNGQKDVNTVLLTPISLTKANLDRVVKDGYFTMAQIEGQ
CCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]