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The map label for this gene is gumJ [H]

Identifier: 162148318

GI number: 162148318

Start: 2631521

End: 2633077

Strand: Reverse

Name: gumJ [H]

Synonym: GDI_2535

Alternate gene names: 162148318

Gene position: 2633077-2631521 (Counterclockwise)

Preceding gene: 162148319

Following gene: 162148317

Centisome position: 66.76

GC content: 67.57

Gene sequence:

>1557_bases
GTGACGACACCGGAAAGACGACGAGACCCAGAGGAGGACCGCATGGCGGCAGAAGGACAGGGCGCGCGCGCGGGCGGGCT
GGGCAGGGCGGCCACCAGCGGGGTCCTGTGGCTGATGGTGCAGAGCCTGGCGGCGCGGGGAATCGGCTTCGTCTCGCAGC
TGGCGCTGGCGTGGCTGCTGACCCCGGCCGATTTCGGGGTCATCGGCCTGGCCTATACCGTGTTCGGGATCGCCAACGCG
CTGGTCAGCTTCGGTGTCGACGACGTGCTGCTGCAACGGCAGAAGGTCATGACGCTGTGGTCGGCGCCGGCCTTCTGGGT
CTGCCTGTCGCTGGGGCTGCTGGGCATGGTCGGGATGCTGGTCGCCGCCCCGTTCGCCGCGTCCTGGTACCATTCGCCGG
AACTGGTGGGGCTGATCGCGGCGCTGGCCATCGCCACGCCGATCAAGACCCTGGCCACCGTGCCTTCGGTGCGCATCCGC
GCCGACATGGATTTCCGCTTCCTGGCGGCCTACAACACATTCGAAATCATGGCGCTGCAACTGGGGACGATCCTGCTGGC
GTGGCTGGGATGCGGCGCCTACAGCTTCGCGATCCCGATTCCGGTGCTGGCCGTCATCAAGGCCGCCCTGTTCTGGTGGA
AGGCCCCGCCGCGCATCGGCCGCGCGATCCGCAAGGTCCAGTTGCAGTACATCCTGGGCAGCAGCAGCACGGTGTTCGCC
ACGCGGACGATCATCGAAATCGTCAACCAGGGCGACTACATCGTCCTGGGGCTGATCGCGTCGCATTCCGTCGTCGGACT
CTATTTCTTCGCCTTCCGCTTCTCGGCGCAGCCGGTGCGGATGCTGGCCGGCAATTTCATCAATGTCCTGCTGCCGGCCT
TCGCGCAGTTGCGCAGCGACCCGCACCGCCAGACCGAGGCCGCGCTGAAGGCCTGTCGCCTGCTGGCCTATATCGTCGTG
CCGTTCTGCTTCCTGCAGGCCGTCCTGGCCGGACCCGGCCTGCATTTCCTGTTCGGCGAACGCTGGATGCGGGCGGTGCC
GTTCGTACAGATCCTCAGCCTGGGCCTGCCGTTCGACGCGGTGTCGTGGATTACCGGCTCGTTGCTCAGCGCGCGGCGCG
AATTCCGGCTGGGGCTGATGTTCGCCGTGATCTCGGCGCCGCTCTTCTTCGGGCTGGCGATCGCGGGCGGCCTGGCGGAC
AGTGCGCTTGGCGTCGCGATGGCGGTGGCGTTCTATTATTTCACCTATCCGCCGCTCTGTTCGCTGCTGATCCTTCGGCG
CTGCGGCGTGCCCGTCGGGCGCGTGATGGAGCTCTACGTCATGCCCTTCGTGATCTCGGCGGTGGCGATGGGGCTGGGGT
ACGCGTCATCGCTCCTGCCAGGCGTCCGCGCCCATGACCTGTCGCGGGTCATCGCGATCGGGACCGTGGGCACCGGCCTC
TATCCGGTGCTGCTGTTCATGCTGCGACGCGACGTGTTCGACCAGCTTGCCGGACGGTTCGGCGGCATGCTGGGGAAGTT
TCGGCGGGTCAAGGGCGGGGTCGCGCAGGCTGCCTAG

Upstream 100 bases:

>100_bases
CGCGCTATCCCGTGCTGCTGGGCCGGATTTCGTTCCGGCTGGTGCTGCCGTCGGGCCTGCGCGACGTCTTCAAGCGCCTG
GCGGGCCGCGCGCAACCGGC

Downstream 100 bases:

>100_bases
AACGGCGCGCCCGCGTTGGGTCCGCCCCCTGCCAGATGACGCAGTCGTTATAGGGTAAGCACGCTATATCGCTTGTATTG
CGCCAACACATCTGCTGTCA

Product: lipopolysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTTPERRRDPEEDRMAAEGQGARAGGLGRAATSGVLWLMVQSLAARGIGFVSQLALAWLLTPADFGVIGLAYTVFGIANA
LVSFGVDDVLLQRQKVMTLWSAPAFWVCLSLGLLGMVGMLVAAPFAASWYHSPELVGLIAALAIATPIKTLATVPSVRIR
ADMDFRFLAAYNTFEIMALQLGTILLAWLGCGAYSFAIPIPVLAVIKAALFWWKAPPRIGRAIRKVQLQYILGSSSTVFA
TRTIIEIVNQGDYIVLGLIASHSVVGLYFFAFRFSAQPVRMLAGNFINVLLPAFAQLRSDPHRQTEAALKACRLLAYIVV
PFCFLQAVLAGPGLHFLFGERWMRAVPFVQILSLGLPFDAVSWITGSLLSARREFRLGLMFAVISAPLFFGLAIAGGLAD
SALGVAMAVAFYYFTYPPLCSLLILRRCGVPVGRVMELYVMPFVISAVAMGLGYASSLLPGVRAHDLSRVIAIGTVGTGL
YPVLLFMLRRDVFDQLAGRFGGMLGKFRRVKGGVAQAA

Sequences:

>Translated_518_residues
MTTPERRRDPEEDRMAAEGQGARAGGLGRAATSGVLWLMVQSLAARGIGFVSQLALAWLLTPADFGVIGLAYTVFGIANA
LVSFGVDDVLLQRQKVMTLWSAPAFWVCLSLGLLGMVGMLVAAPFAASWYHSPELVGLIAALAIATPIKTLATVPSVRIR
ADMDFRFLAAYNTFEIMALQLGTILLAWLGCGAYSFAIPIPVLAVIKAALFWWKAPPRIGRAIRKVQLQYILGSSSTVFA
TRTIIEIVNQGDYIVLGLIASHSVVGLYFFAFRFSAQPVRMLAGNFINVLLPAFAQLRSDPHRQTEAALKACRLLAYIVV
PFCFLQAVLAGPGLHFLFGERWMRAVPFVQILSLGLPFDAVSWITGSLLSARREFRLGLMFAVISAPLFFGLAIAGGLAD
SALGVAMAVAFYYFTYPPLCSLLILRRCGVPVGRVMELYVMPFVISAVAMGLGYASSLLPGVRAHDLSRVIAIGTVGTGL
YPVLLFMLRRDVFDQLAGRFGGMLGKFRRVKGGVAQAA
>Mature_517_residues
TTPERRRDPEEDRMAAEGQGARAGGLGRAATSGVLWLMVQSLAARGIGFVSQLALAWLLTPADFGVIGLAYTVFGIANAL
VSFGVDDVLLQRQKVMTLWSAPAFWVCLSLGLLGMVGMLVAAPFAASWYHSPELVGLIAALAIATPIKTLATVPSVRIRA
DMDFRFLAAYNTFEIMALQLGTILLAWLGCGAYSFAIPIPVLAVIKAALFWWKAPPRIGRAIRKVQLQYILGSSSTVFAT
RTIIEIVNQGDYIVLGLIASHSVVGLYFFAFRFSAQPVRMLAGNFINVLLPAFAQLRSDPHRQTEAALKACRLLAYIVVP
FCFLQAVLAGPGLHFLFGERWMRAVPFVQILSLGLPFDAVSWITGSLLSARREFRLGLMFAVISAPLFFGLAIAGGLADS
ALGVAMAVAFYYFTYPPLCSLLILRRCGVPVGRVMELYVMPFVISAVAMGLGYASSLLPGVRAHDLSRVIAIGTVGTGLY
PVLLFMLRRDVFDQLAGRFGGMLGKFRRVKGGVAQAA

Specific function: Lipopolysaccharide biosynthesis. [C]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788359, Length=388, Percent_Identity=22.680412371134, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 55923; Mature: 55792

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPERRRDPEEDRMAAEGQGARAGGLGRAATSGVLWLMVQSLAARGIGFVSQLALAWLL
CCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TPADFGVIGLAYTVFGIANALVSFGVDDVLLQRQKVMTLWSAPAFWVCLSLGLLGMVGML
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VAAPFAASWYHSPELVGLIAALAIATPIKTLATVPSVRIRADMDFRFLAAYNTFEIMALQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHHHH
LGTILLAWLGCGAYSFAIPIPVLAVIKAALFWWKAPPRIGRAIRKVQLQYILGSSSTVFA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHH
TRTIIEIVNQGDYIVLGLIASHSVVGLYFFAFRFSAQPVRMLAGNFINVLLPAFAQLRSD
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PHRQTEAALKACRLLAYIVVPFCFLQAVLAGPGLHFLFGERWMRAVPFVQILSLGLPFDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VSWITGSLLSARREFRLGLMFAVISAPLFFGLAIAGGLADSALGVAMAVAFYYFTYPPLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLILRRCGVPVGRVMELYVMPFVISAVAMGLGYASSLLPGVRAHDLSRVIAIGTVGTGL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCH
YPVLLFMLRRDVFDQLAGRFGGMLGKFRRVKGGVAQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
TTPERRRDPEEDRMAAEGQGARAGGLGRAATSGVLWLMVQSLAARGIGFVSQLALAWLL
CCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TPADFGVIGLAYTVFGIANALVSFGVDDVLLQRQKVMTLWSAPAFWVCLSLGLLGMVGML
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VAAPFAASWYHSPELVGLIAALAIATPIKTLATVPSVRIRADMDFRFLAAYNTFEIMALQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHHHH
LGTILLAWLGCGAYSFAIPIPVLAVIKAALFWWKAPPRIGRAIRKVQLQYILGSSSTVFA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHH
TRTIIEIVNQGDYIVLGLIASHSVVGLYFFAFRFSAQPVRMLAGNFINVLLPAFAQLRSD
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PHRQTEAALKACRLLAYIVVPFCFLQAVLAGPGLHFLFGERWMRAVPFVQILSLGLPFDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VSWITGSLLSARREFRLGLMFAVISAPLFFGLAIAGGLADSALGVAMAVAFYYFTYPPLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLILRRCGVPVGRVMELYVMPFVISAVAMGLGYASSLLPGVRAHDLSRVIAIGTVGTGL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCH
YPVLLFMLRRDVFDQLAGRFGGMLGKFRRVKGGVAQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]