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The map label for this gene is ilvE [H]

Identifier: 162148262

GI number: 162148262

Start: 2572522

End: 2573616

Strand: Reverse

Name: ilvE [H]

Synonym: GDI_2479

Alternate gene names: 162148262

Gene position: 2573616-2572522 (Counterclockwise)

Preceding gene: 162148263

Following gene: 162148260

Centisome position: 65.25

GC content: 67.49

Gene sequence:

>1095_bases
ATGGACAGCGCCGACCAATTTCGTTTTACGCATCTTCCGCATCCCGCGCCGGTTCCGGCGGCACGGCGCACGGAATTGCT
GGCCAATCCGGGATTCGGTCGCGTCTTCACCGACCACATGGCTGTCGTACGCTACAAGGAAGGGCAGGGCTGGCATAACC
CGACGATCGAGGCCCGGGCCCCGATCCCGCTGGACCCGGCCGCGGCGGTGCTGCACTACGCCCAGGAAATTTTCGAGGGC
ATGAAGGCCTATCGCGCCGCCGATGGCGGCGTGGTGATGTTCCGGCCCGATGCGAATGCCCGCCGGTTCTATCAGTCCGC
CGAACGCATGGCGATGGCCCCGGTGCCGGATGCGCTGTTCCTGGCGGCGGTGCGGGAACTGGTGCGGGTCGACCGCGACT
GGATTCCGACCGGCGAGAACGCCAGCCTGTACCTGCGGCCGTTCATGATCGCCAGCGAGGCGTTCCTGGGCGTGAAGCCC
TCGTCGGAATATCTGTTCATCGTCATCGCCTCGCCGGTCGGATCGTATTTCAGCGGGGGCGCCGTCTCCGTCTGGGTGTC
CGAGGATTATACCCGCGCGGCCGTGGGCGGCACGGGGGCGGCCAAATGCGGTGGCAACTATGCCGCAAGCCTGCTGGCCC
AGCGCGAGGCGAAGACCCACGGGTGCGATCAGGTCCTGTTCCTGGACGCCGTCGAACACCGATGGGTCGAGGAAATGGGC
GGCATGAACATTTTCTTCGTGCTGGATGACGGATCGCTGGTCACCCCGCCGCTGGGGGGCACGATCCTGCCGGGCATCAC
CCGCGACGCGATCCTGACACTGGCGCGCGACCGTGGCCTGACGGTGCGCGAGGAGCCGTACAGCATCGACCAGTGCTATG
CGGACGCCGCCAGCGGTCGCCTGAAGGAAGCCTTCGCCTGCGGAACGGCGGCGGTCGTGACCCCGATCGGCCGCCTGTGC
GGCCATAGGGGCGAGGTCGTGATCGGCAGCGGCAACGGCGCCGATCCGATCACCGAAAGCCTGCGCGGCGCATTGATCGG
CATCCAGCGCGGGCAGAACCCGGACCCCTATGGCTGGGTCCAGCACGTCGCCTGA

Upstream 100 bases:

>100_bases
TCTGACCGATCGGTCCGGCCCGCGTCGCGGGCGCGTAAGGGGGCCGTTTCGGGCTGGCGCGGCAGAGGCCGCGCCGATGT
GGTGGTGAAAGAGCAGGATC

Downstream 100 bases:

>100_bases
ACCCAGGACGACCAGCTACCCCAAAGGGCTCGGCCCTTTGGGGCACAGGGCAGCGCCCTGACGCTTTACAAGGCTGCGAT
GGCAGTGAGTTCGACCCGCC

Product: branched-chain-amino-acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MDSADQFRFTHLPHPAPVPAARRTELLANPGFGRVFTDHMAVVRYKEGQGWHNPTIEARAPIPLDPAAAVLHYAQEIFEG
MKAYRAADGGVVMFRPDANARRFYQSAERMAMAPVPDALFLAAVRELVRVDRDWIPTGENASLYLRPFMIASEAFLGVKP
SSEYLFIVIASPVGSYFSGGAVSVWVSEDYTRAAVGGTGAAKCGGNYAASLLAQREAKTHGCDQVLFLDAVEHRWVEEMG
GMNIFFVLDDGSLVTPPLGGTILPGITRDAILTLARDRGLTVREEPYSIDQCYADAASGRLKEAFACGTAAVVTPIGRLC
GHRGEVVIGSGNGADPITESLRGALIGIQRGQNPDPYGWVQHVA

Sequences:

>Translated_364_residues
MDSADQFRFTHLPHPAPVPAARRTELLANPGFGRVFTDHMAVVRYKEGQGWHNPTIEARAPIPLDPAAAVLHYAQEIFEG
MKAYRAADGGVVMFRPDANARRFYQSAERMAMAPVPDALFLAAVRELVRVDRDWIPTGENASLYLRPFMIASEAFLGVKP
SSEYLFIVIASPVGSYFSGGAVSVWVSEDYTRAAVGGTGAAKCGGNYAASLLAQREAKTHGCDQVLFLDAVEHRWVEEMG
GMNIFFVLDDGSLVTPPLGGTILPGITRDAILTLARDRGLTVREEPYSIDQCYADAASGRLKEAFACGTAAVVTPIGRLC
GHRGEVVIGSGNGADPITESLRGALIGIQRGQNPDPYGWVQHVA
>Mature_364_residues
MDSADQFRFTHLPHPAPVPAARRTELLANPGFGRVFTDHMAVVRYKEGQGWHNPTIEARAPIPLDPAAAVLHYAQEIFEG
MKAYRAADGGVVMFRPDANARRFYQSAERMAMAPVPDALFLAAVRELVRVDRDWIPTGENASLYLRPFMIASEAFLGVKP
SSEYLFIVIASPVGSYFSGGAVSVWVSEDYTRAAVGGTGAAKCGGNYAASLLAQREAKTHGCDQVLFLDAVEHRWVEEMG
GMNIFFVLDDGSLVTPPLGGTILPGITRDAILTLARDRGLTVREEPYSIDQCYADAASGRLKEAFACGTAAVVTPIGRLC
GHRGEVVIGSGNGADPITESLRGALIGIQRGQNPDPYGWVQHVA

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=333, Percent_Identity=40.5405405405405, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI296010904, Length=333, Percent_Identity=40.5405405405405, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI38176287, Length=333, Percent_Identity=40.5405405405405, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI50658084, Length=354, Percent_Identity=37.5706214689266, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI296010902, Length=333, Percent_Identity=36.3363363363363, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI296010900, Length=333, Percent_Identity=36.3363363363363, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI258614015, Length=284, Percent_Identity=38.3802816901408, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI48994963, Length=300, Percent_Identity=35, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17568601, Length=299, Percent_Identity=40.133779264214, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17565728, Length=299, Percent_Identity=37.4581939799331, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322608, Length=335, Percent_Identity=39.1044776119403, Blast_Score=228, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6322002, Length=327, Percent_Identity=40.6727828746177, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24641779, Length=339, Percent_Identity=42.7728613569322, Blast_Score=248, Evalue=5e-66,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39233; Mature: 39233

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSADQFRFTHLPHPAPVPAARRTELLANPGFGRVFTDHMAVVRYKEGQGWHNPTIEARA
CCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCC
PIPLDPAAAVLHYAQEIFEGMKAYRAADGGVVMFRPDANARRFYQSAERMAMAPVPDALF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHH
LAAVRELVRVDRDWIPTGENASLYLRPFMIASEAFLGVKPSSEYLFIVIASPVGSYFSGG
HHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHCCCCCCCCEEEEEEECCCCCHHCCC
AVSVWVSEDYTRAAVGGTGAAKCGGNYAASLLAQREAKTHGCDQVLFLDAVEHRWVEEMG
EEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHC
GMNIFFVLDDGSLVTPPLGGTILPGITRDAILTLARDRGLTVREEPYSIDQCYADAASGR
CCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCH
LKEAFACGTAAVVTPIGRLCGHRGEVVIGSGNGADPITESLRGALIGIQRGQNPDPYGWV
HHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHEEEECCCCCCCCCCHH
QHVA
HCCC
>Mature Secondary Structure
MDSADQFRFTHLPHPAPVPAARRTELLANPGFGRVFTDHMAVVRYKEGQGWHNPTIEARA
CCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCC
PIPLDPAAAVLHYAQEIFEGMKAYRAADGGVVMFRPDANARRFYQSAERMAMAPVPDALF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHH
LAAVRELVRVDRDWIPTGENASLYLRPFMIASEAFLGVKPSSEYLFIVIASPVGSYFSGG
HHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHCCCCCCCCEEEEEEECCCCCHHCCC
AVSVWVSEDYTRAAVGGTGAAKCGGNYAASLLAQREAKTHGCDQVLFLDAVEHRWVEEMG
EEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHC
GMNIFFVLDDGSLVTPPLGGTILPGITRDAILTLARDRGLTVREEPYSIDQCYADAASGR
CCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCH
LKEAFACGTAAVVTPIGRLCGHRGEVVIGSGNGADPITESLRGALIGIQRGQNPDPYGWV
HHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHEEEECCCCCCCCCCHH
QHVA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]