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The map label for this gene is plcN [H]

Identifier: 162148260

GI number: 162148260

Start: 2569290

End: 2571776

Strand: Reverse

Name: plcN [H]

Synonym: GDI_2477

Alternate gene names: 162148260

Gene position: 2571776-2569290 (Counterclockwise)

Preceding gene: 162148262

Following gene: 162148256

Centisome position: 65.2

GC content: 63.93

Gene sequence:

>2487_bases
ATGGCCACACGCAGAGATTTCCTGAAATACGCCCTTCTGTCCGGAGCGGCGGGTGGCGCGTCGTTGCTGTCTGATCCCAT
CCGCCGTGCTTTCGCCATTGCACCGAATCCGGGCTCGACCTGGCAGGACGCCGAGCATGTCGTGATCCTGATGCAGGAAA
ACCGCTCGTTCGACCACGTCTTCGGCACGCTGCAGGGTGTGCGCGGCTTCAACGACCCGCGTGCGATGCGCCTGGCCAAC
GGCAATCCGGTCTTTGCGCAGACCGGCAAGGCGGGGGAGACGTATCTGCCCTGGCGGCTGAACATCCGCGATACGCGCGT
GACCTGGATGGGCTCGATCCCCCATTCCCGCGACAGCCAGGTCGATGCCTGGAATGGCGGCGGTCATGACGGATGGATTG
ATGCCAAGAAGTCGCATCACAAGGGCTATGATCGCTATCCGCTGACAATGGGCCACTACACGCGCGAGGACCTGCCTTTT
TACTACGCGCTGGCCGACGCCTTTACCGTCTGCGACCAGAATTACTGCGGCGCGATGACCAGTACCACGCCCAATCGGCT
GCTGTTCATGACCGGAACCGTGCGCGACCGGCAGGATACGTCGTCGAACGTGTATATGCGCAATGGCGAGATCCTGGAGG
GCGGCATGACGTGGGCCACCTTTCCGGAACGACTGGAACAGGCCGGAATTTCGTGGAAATACTACCAGAACGAACTGACG
CAGACCGGCGGCATGAGCGAGCCGGAACGGGCGTGGCTGGGGAATTTCGGCACGAACGTTCTGGAATGCTTCGATCAGTA
TTGCGTTTCGGCCAATCCCGGTTTCGCCGACTGGCTCGAGGCGCGCATCGCCGACTGCCAGGGCCATATCGACCGGCTCA
ACAGCCGGGAAATGCTCATTTCCGAAGCGCATGAGGAGCAGTTGGCCGAGGCGCGGATGCTGCTCGATGTCCTGACGCGC
CGTCGCGGCCTGGCCGACCAGACGATGGACAGGCTGACACCGCAGGAACGTACGTTGTTCGAGAAGGCGTTCGTCATCAA
CAGCGGTGATCCGAATTATCGCAAGCTGGACCGTCTGGCCTTCCATGACGGAGAAAAGACCACTCACATGGATGCGCCGA
AGGGCGACCTCCTGCATCAGTTCCGCCAGGATGTGAAAGCGGGCAGCCTGCCTGTCGTATCGTGGCTGTCGGCACCGGGG
CATTTTTCCGACCATCCGACCTCGCCGTGGTACGGCGCGTGGTACGTGTCGGAAGTCATGGATATCCTGACCGAAAATCC
CGATATCTGGAAAAAGACCATTTTCATCATCACCTATGATGAAAATGACGGTTATTTCGACCATTGCTGTTCCTACGCGG
CCCCGGACCCCACGCGCCCGGACACCGGTCGTTCTTCCGCCGGCATCGGACCGGACGGGCTGGAATACACCACCGCGAAG
GATGAAACGGACCGAGGCGTGCCCGAACGCCTGGCCCGCAGCGGCCCGATCGGGCTGGGCTTCCGTGTGCCGATGATCGT
GGCCTCGCCCTGGAGCCGGGGCGGTTGGGTCAATTCCCAACTGTTCGAACACACCTCGACGTTGCGCTTTCTGGAAGCCT
TCATCCAGGGCAAGTTCGGCAGGACGGTCACCGAGACCAATATCTCGCCCTGGCGCCGCGCCATCAGCGGCGACCTGACC
TCCTGCTTCCGGCCCTATGACGGTACGGTGCCGACCCTGCCTTTCCTGGACCGCGACGCCCATCTGCATATGATAGAGGA
CGCGCGTGACCGGCCGATGCCGGGCGGCTTTCGCGCGCTGGATGATGCGGAGATAGCCGCCCTGCGCGCCGACCCCGCCA
CCTTGCGGCGGGTGATCCGGCAGGAGCCCGGCACGCGTCCGGCCTGCGCTCTGCCCTACGAGCCCTATTGCGACGGCGGC
ATGTCGAAGGATGGCAGGCTGGTCAGCCTGCGTATGCGCGCCGGTACGACGCTGCATGGCGCACGGTCGGCCGGGCTGCC
GTTCAATGTCTATCGTCATGCCGCCGCACCCGATGGCGGCATGCAGGCAGGGACCTACGCCGTCGCGGCCGGGGATACGC
TGAGCACGGCATTCGGCGGTTCGGCATTTCTCGACGGGCTCTACGACGTGTCGGTCCATGCGCCGAACGGCTTTTATCGT
CGGTACAGGGGGCGGCGGGACGGCGTGCCGTTATGGGCGGAATGCCTCTATCTGCACGGCAAGGATGCGGATCTGCTGGT
GCGCGTCACCAATCACGGGACGAAGGCAATCGACATAACCTGTACCCTGGCCGCGCACGGCACGGCGCGGACGCTGCATG
TGCCGCCGGGACGGAGCGTCGAGGCGCCGTTCGCCCTGCGTCAGGACGCGATGTGGTACGACGTCACCCTGACCAGCCCA
ACCGAGGGCGATCTGCTGTATCAGTTCGCGGGCCGGGTCGAAACCGGGAACCCGGGGCGGACCGATCCGGCCATGGGCGC
GGCGTAG

Upstream 100 bases:

>100_bases
ATTGTCATAAGGGAAACCGGGAGGTCTGTCGTATAGGCACTGACCTATACGACACTCATCTTATATGCCCCTCATCCGGC
GGGTCTTGCGCGAGTAGACA

Downstream 100 bases:

>100_bases
GGGACCATCTCCCCGCGCGTCAGCGGGCGGGGCGATCGGTCAGCATGTCCAGGAAGGCGACGATGTCCCGCCGCTCGTCC
GGGGTCAGGGGCGGCGCGTC

Product: non-hemolytic phospholipase C

Products: NA

Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]

Number of amino acids: Translated: 828; Mature: 827

Protein sequence:

>828_residues
MATRRDFLKYALLSGAAGGASLLSDPIRRAFAIAPNPGSTWQDAEHVVILMQENRSFDHVFGTLQGVRGFNDPRAMRLAN
GNPVFAQTGKAGETYLPWRLNIRDTRVTWMGSIPHSRDSQVDAWNGGGHDGWIDAKKSHHKGYDRYPLTMGHYTREDLPF
YYALADAFTVCDQNYCGAMTSTTPNRLLFMTGTVRDRQDTSSNVYMRNGEILEGGMTWATFPERLEQAGISWKYYQNELT
QTGGMSEPERAWLGNFGTNVLECFDQYCVSANPGFADWLEARIADCQGHIDRLNSREMLISEAHEEQLAEARMLLDVLTR
RRGLADQTMDRLTPQERTLFEKAFVINSGDPNYRKLDRLAFHDGEKTTHMDAPKGDLLHQFRQDVKAGSLPVVSWLSAPG
HFSDHPTSPWYGAWYVSEVMDILTENPDIWKKTIFIITYDENDGYFDHCCSYAAPDPTRPDTGRSSAGIGPDGLEYTTAK
DETDRGVPERLARSGPIGLGFRVPMIVASPWSRGGWVNSQLFEHTSTLRFLEAFIQGKFGRTVTETNISPWRRAISGDLT
SCFRPYDGTVPTLPFLDRDAHLHMIEDARDRPMPGGFRALDDAEIAALRADPATLRRVIRQEPGTRPACALPYEPYCDGG
MSKDGRLVSLRMRAGTTLHGARSAGLPFNVYRHAAAPDGGMQAGTYAVAAGDTLSTAFGGSAFLDGLYDVSVHAPNGFYR
RYRGRRDGVPLWAECLYLHGKDADLLVRVTNHGTKAIDITCTLAAHGTARTLHVPPGRSVEAPFALRQDAMWYDVTLTSP
TEGDLLYQFAGRVETGNPGRTDPAMGAA

Sequences:

>Translated_828_residues
MATRRDFLKYALLSGAAGGASLLSDPIRRAFAIAPNPGSTWQDAEHVVILMQENRSFDHVFGTLQGVRGFNDPRAMRLAN
GNPVFAQTGKAGETYLPWRLNIRDTRVTWMGSIPHSRDSQVDAWNGGGHDGWIDAKKSHHKGYDRYPLTMGHYTREDLPF
YYALADAFTVCDQNYCGAMTSTTPNRLLFMTGTVRDRQDTSSNVYMRNGEILEGGMTWATFPERLEQAGISWKYYQNELT
QTGGMSEPERAWLGNFGTNVLECFDQYCVSANPGFADWLEARIADCQGHIDRLNSREMLISEAHEEQLAEARMLLDVLTR
RRGLADQTMDRLTPQERTLFEKAFVINSGDPNYRKLDRLAFHDGEKTTHMDAPKGDLLHQFRQDVKAGSLPVVSWLSAPG
HFSDHPTSPWYGAWYVSEVMDILTENPDIWKKTIFIITYDENDGYFDHCCSYAAPDPTRPDTGRSSAGIGPDGLEYTTAK
DETDRGVPERLARSGPIGLGFRVPMIVASPWSRGGWVNSQLFEHTSTLRFLEAFIQGKFGRTVTETNISPWRRAISGDLT
SCFRPYDGTVPTLPFLDRDAHLHMIEDARDRPMPGGFRALDDAEIAALRADPATLRRVIRQEPGTRPACALPYEPYCDGG
MSKDGRLVSLRMRAGTTLHGARSAGLPFNVYRHAAAPDGGMQAGTYAVAAGDTLSTAFGGSAFLDGLYDVSVHAPNGFYR
RYRGRRDGVPLWAECLYLHGKDADLLVRVTNHGTKAIDITCTLAAHGTARTLHVPPGRSVEAPFALRQDAMWYDVTLTSP
TEGDLLYQFAGRVETGNPGRTDPAMGAA
>Mature_827_residues
ATRRDFLKYALLSGAAGGASLLSDPIRRAFAIAPNPGSTWQDAEHVVILMQENRSFDHVFGTLQGVRGFNDPRAMRLANG
NPVFAQTGKAGETYLPWRLNIRDTRVTWMGSIPHSRDSQVDAWNGGGHDGWIDAKKSHHKGYDRYPLTMGHYTREDLPFY
YALADAFTVCDQNYCGAMTSTTPNRLLFMTGTVRDRQDTSSNVYMRNGEILEGGMTWATFPERLEQAGISWKYYQNELTQ
TGGMSEPERAWLGNFGTNVLECFDQYCVSANPGFADWLEARIADCQGHIDRLNSREMLISEAHEEQLAEARMLLDVLTRR
RGLADQTMDRLTPQERTLFEKAFVINSGDPNYRKLDRLAFHDGEKTTHMDAPKGDLLHQFRQDVKAGSLPVVSWLSAPGH
FSDHPTSPWYGAWYVSEVMDILTENPDIWKKTIFIITYDENDGYFDHCCSYAAPDPTRPDTGRSSAGIGPDGLEYTTAKD
ETDRGVPERLARSGPIGLGFRVPMIVASPWSRGGWVNSQLFEHTSTLRFLEAFIQGKFGRTVTETNISPWRRAISGDLTS
CFRPYDGTVPTLPFLDRDAHLHMIEDARDRPMPGGFRALDDAEIAALRADPATLRRVIRQEPGTRPACALPYEPYCDGGM
SKDGRLVSLRMRAGTTLHGARSAGLPFNVYRHAAAPDGGMQAGTYAVAAGDTLSTAFGGSAFLDGLYDVSVHAPNGFYRR
YRGRRDGVPLWAECLYLHGKDADLLVRVTNHGTKAIDITCTLAAHGTARTLHVPPGRSVEAPFALRQDAMWYDVTLTSPT
EGDLLYQFAGRVETGNPGRTDPAMGAA

Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR008475
- InterPro:   IPR017767
- InterPro:   IPR006311 [H]

Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 91914; Mature: 91783

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRRDFLKYALLSGAAGGASLLSDPIRRAFAIAPNPGSTWQDAEHVVILMQENRSFDHV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEEECCCCHHHH
FGTLQGVRGFNDPRAMRLANGNPVFAQTGKAGETYLPWRLNIRDTRVTWMGSIPHSRDSQ
HHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCEECCEEEEEEEEEEEEEECCCCCCCCC
VDAWNGGGHDGWIDAKKSHHKGYDRYPLTMGHYTREDLPFYYALADAFTVCDQNYCGAMT
CCCCCCCCCCCCCCCCHHHCCCCCCCCEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCC
STTPNRLLFMTGTVRDRQDTSSNVYMRNGEILEGGMTWATFPERLEQAGISWKYYQNELT
CCCCCEEEEEECCCCCCCCCCCCEEEECCEEEECCCCHHHHHHHHHHCCCCEEEHHHHHH
QTGGMSEPERAWLGNFGTNVLECFDQYCVSANPGFADWLEARIADCQGHIDRLNSREMLI
HCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
SEAHEEQLAEARMLLDVLTRRRGLADQTMDRLTPQERTLFEKAFVINSGDPNYRKLDRLA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHEEEEECCCCCHHHHHHHH
FHDGEKTTHMDAPKGDLLHQFRQDVKAGSLPVVSWLSAPGHFSDHPTSPWYGAWYVSEVM
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
DILTENPDIWKKTIFIITYDENDGYFDHCCSYAAPDPTRPDTGRSSAGIGPDGLEYTTAK
HHHHCCCCCEEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCC
DETDRGVPERLARSGPIGLGFRVPMIVASPWSRGGWVNSQLFEHTSTLRFLEAFIQGKFG
CCCCCCCHHHHHHCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
RTVTETNISPWRRAISGDLTSCFRPYDGTVPTLPFLDRDAHLHMIEDARDRPMPGGFRAL
CEEEECCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
DDAEIAALRADPATLRRVIRQEPGTRPACALPYEPYCDGGMSKDGRLVSLRMRAGTTLHG
CCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCEECC
ARSAGLPFNVYRHAAAPDGGMQAGTYAVAAGDTLSTAFGGSAFLDGLYDVSVHAPNGFYR
CCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCHHHHHCCCHHHHCCEEEEEEECCCHHHH
RYRGRRDGVPLWAECLYLHGKDADLLVRVTNHGTKAIDITCTLAAHGTARTLHVPPGRSV
HHCCCCCCCCHHHHEEEECCCCCEEEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCC
EAPFALRQDAMWYDVTLTSPTEGDLLYQFAGRVETGNPGRTDPAMGAA
CCCHHHCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATRRDFLKYALLSGAAGGASLLSDPIRRAFAIAPNPGSTWQDAEHVVILMQENRSFDHV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEEECCCCHHHH
FGTLQGVRGFNDPRAMRLANGNPVFAQTGKAGETYLPWRLNIRDTRVTWMGSIPHSRDSQ
HHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCEECCEEEEEEEEEEEEEECCCCCCCCC
VDAWNGGGHDGWIDAKKSHHKGYDRYPLTMGHYTREDLPFYYALADAFTVCDQNYCGAMT
CCCCCCCCCCCCCCCCHHHCCCCCCCCEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCC
STTPNRLLFMTGTVRDRQDTSSNVYMRNGEILEGGMTWATFPERLEQAGISWKYYQNELT
CCCCCEEEEEECCCCCCCCCCCCEEEECCEEEECCCCHHHHHHHHHHCCCCEEEHHHHHH
QTGGMSEPERAWLGNFGTNVLECFDQYCVSANPGFADWLEARIADCQGHIDRLNSREMLI
HCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
SEAHEEQLAEARMLLDVLTRRRGLADQTMDRLTPQERTLFEKAFVINSGDPNYRKLDRLA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHEEEEECCCCCHHHHHHHH
FHDGEKTTHMDAPKGDLLHQFRQDVKAGSLPVVSWLSAPGHFSDHPTSPWYGAWYVSEVM
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
DILTENPDIWKKTIFIITYDENDGYFDHCCSYAAPDPTRPDTGRSSAGIGPDGLEYTTAK
HHHHCCCCCEEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCC
DETDRGVPERLARSGPIGLGFRVPMIVASPWSRGGWVNSQLFEHTSTLRFLEAFIQGKFG
CCCCCCCHHHHHHCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
RTVTETNISPWRRAISGDLTSCFRPYDGTVPTLPFLDRDAHLHMIEDARDRPMPGGFRAL
CEEEECCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
DDAEIAALRADPATLRRVIRQEPGTRPACALPYEPYCDGGMSKDGRLVSLRMRAGTTLHG
CCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCEECC
ARSAGLPFNVYRHAAAPDGGMQAGTYAVAAGDTLSTAFGGSAFLDGLYDVSVHAPNGFYR
CCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCHHHHHCCCHHHHCCEEEEEEECCCHHHH
RYRGRRDGVPLWAECLYLHGKDADLLVRVTNHGTKAIDITCTLAAHGTARTLHVPPGRSV
HHCCCCCCCCHHHHEEEECCCCCEEEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCC
EAPFALRQDAMWYDVTLTSPTEGDLLYQFAGRVETGNPGRTDPAMGAA
CCCHHHCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2120196; 10984043 [H]