Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 162148253

GI number: 162148253

Start: 2561527

End: 2562162

Strand: Reverse

Name: nuoB

Synonym: GDI_2470

Alternate gene names: 162148253

Gene position: 2562162-2561527 (Counterclockwise)

Preceding gene: 162148254

Following gene: 162148252

Centisome position: 64.96

GC content: 65.88

Gene sequence:

>636_bases
ATGCGCTGGTCGCTGACCCGACCGGGCGAGGAACCCGGCACGCCGCGCGACGCGACCATGACCGAAACCGTGAAACGCAA
CCTGGTGATGGGGCGGTTGCAGGATTTCGTGTCCTGGGGACAGAAGAATTCGATCTGGCCGTTCAATTTCGGCCTGTCCT
GCTGCTACGTCGAAATGGCGACGGCCTTCACCAGCAAATACGACATCGCCCGGTTCGGGTCCGAGGTGCTGCGCGCCACC
CCGCGCGAGGCCGACCTGATCGTCATCTCCGGCACCGTCTTCGTGAAGATGGCCCCGATCGTCAAATATCTGTACGACCA
GATGCTGGAACCGCGCTGGGTGATCTCGATGGGGTCCTGCGCCAATTCCGGCGGAATGTACGACATCTACAGCGTGGTGC
AGGGGGTGGACAGCTTCCTGCCGGTCGATGTCTACGTCCCGGGCTGCCCGCCCCGGCCGGATGCGCTGCTGGAAGGACTG
ATGCTGCTGCAGGATTCCGTGGGCACGCAGCGCCGGCCGCTCAGCTGGATGGTGGGGCCGCAGGGTGTCGAACGGGTCAC
GCCGCCCAGCCTGCGTGATGCGAAGCGCGACCAGCGCCGCGCCGCGCAGACCCTGCGCAGCCCCGACACGATCTGA

Upstream 100 bases:

>100_bases
CGCTGGCGGCGGCGCTGGCCTATGTGTGGCGGTGGGGCGCGCTGGACTGGGGCCCTGTTCCCCGTCGCCGGATAGACTAT
CGCAGGGCGGGGGACGCATC

Downstream 100 bases:

>100_bases
CGGGGCCGCGCGATGATCGTGCCCGACCTGGTGGCGGACGCCATGACCGCAGGACCGCCCGCCGCGAAAAGCGGAGACGG
CGCCACCATGTTCGGGCAGG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRWSLTRPGEEPGTPRDATMTETVKRNLVMGRLQDFVSWGQKNSIWPFNFGLSCCYVEMATAFTSKYDIARFGSEVLRAT
PREADLIVISGTVFVKMAPIVKYLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDSFLPVDVYVPGCPPRPDALLEGL
MLLQDSVGTQRRPLSWMVGPQGVERVTPPSLRDAKRDQRRAAQTLRSPDTI

Sequences:

>Translated_211_residues
MRWSLTRPGEEPGTPRDATMTETVKRNLVMGRLQDFVSWGQKNSIWPFNFGLSCCYVEMATAFTSKYDIARFGSEVLRAT
PREADLIVISGTVFVKMAPIVKYLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDSFLPVDVYVPGCPPRPDALLEGL
MLLQDSVGTQRRPLSWMVGPQGVERVTPPSLRDAKRDQRRAAQTLRSPDTI
>Mature_211_residues
MRWSLTRPGEEPGTPRDATMTETVKRNLVMGRLQDFVSWGQKNSIWPFNFGLSCCYVEMATAFTSKYDIARFGSEVLRAT
PREADLIVISGTVFVKMAPIVKYLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDSFLPVDVYVPGCPPRPDALLEGL
MLLQDSVGTQRRPLSWMVGPQGVERVTPPSLRDAKRDQRRAAQTLRSPDTI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=149, Percent_Identity=50.3355704697987, Blast_Score=167, Evalue=5e-42,
Organism=Escherichia coli, GI1788624, Length=192, Percent_Identity=66.6666666666667, Blast_Score=292, Evalue=1e-80,
Organism=Escherichia coli, GI1788834, Length=200, Percent_Identity=34, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1789074, Length=113, Percent_Identity=38.0530973451327, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=165, Percent_Identity=49.0909090909091, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI18859983, Length=174, Percent_Identity=47.7011494252874, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24642371, Length=174, Percent_Identity=47.7011494252874, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=53.0612244897959, Blast_Score=166, Evalue=7e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB1_GLUDA (A9HN98)

Other databases:

- EMBL:   CP001189
- EMBL:   AM889285
- RefSeq:   YP_001602714.1
- RefSeq:   YP_002275122.1
- ProteinModelPortal:   A9HN98
- SMR:   A9HN98
- GeneID:   5789120
- GeneID:   6974114
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0717
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- ProtClustDB:   PRK06411
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 23631; Mature: 23631

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSLTRPGEEPGTPRDATMTETVKRNLVMGRLQDFVSWGQKNSIWPFNFGLSCCYVEMA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHH
TAFTSKYDIARFGSEVLRATPREADLIVISGTVFVKMAPIVKYLYDQMLEPRWVISMGSC
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
ANSGGMYDIYSVVQGVDSFLPVDVYVPGCPPRPDALLEGLMLLQDSVGTQRRPLSWMVGP
CCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCC
QGVERVTPPSLRDAKRDQRRAAQTLRSPDTI
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRWSLTRPGEEPGTPRDATMTETVKRNLVMGRLQDFVSWGQKNSIWPFNFGLSCCYVEMA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHH
TAFTSKYDIARFGSEVLRATPREADLIVISGTVFVKMAPIVKYLYDQMLEPRWVISMGSC
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
ANSGGMYDIYSVVQGVDSFLPVDVYVPGCPPRPDALLEGLMLLQDSVGTQRRPLSWMVGP
CCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCC
QGVERVTPPSLRDAKRDQRRAAQTLRSPDTI
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA