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The map label for this gene is rbsB [H]

Identifier: 162148225

GI number: 162148225

Start: 2532166

End: 2533173

Strand: Direct

Name: rbsB [H]

Synonym: GDI_2442

Alternate gene names: 162148225

Gene position: 2532166-2533173 (Clockwise)

Preceding gene: 162148224

Following gene: 162148226

Centisome position: 64.2

GC content: 62.6

Gene sequence:

>1008_bases
GTGAAATCATCCGTTGAAACCAAAAACAAGAACAAGAAAGACAAAACGATGATTGTCAGGATTGCCCCTGTCGTCGGCCT
GGCGCTCCTCTTCCCGCTGGCGGCGGCGGCCGCCCCGCTGGCGAAGGAGTGCGGGCCGAACAAGAGCTATGTCATCGGGT
TCTCGCAGGCCAACTTCAAGGAACCGTACCGGGCGCACGTGGATCATGAACTTCAGCGCCTGGTGAAGAATTATCCGCAA
TTCAAGCTCGTCATCGCCGACGGCCAGGCCAACGACAATACCCAGGTCTCGCAGGTCGAGAATTTCCTGACGCAGCAGGT
CGATATCCTGTTCGTCTCGGCGTTCGAGGCCGCGCCGCTGACCCCGGCCGTCAGTGCCGTCTACGACAAGGGTATCCCGG
TCATCGAACTGGACCGCAGGACGACGGGCGAAAAATTCACCAGCTTCGTCAGCGGCGATAATCGCGCCATCGCGCATGAA
GCGGGCCTGTATGCGGCAAGCCTGCTGCCCGATGGCGGCGACGTCGCGATTCTGGAGGGGCTGCCCAGTTCCTCGCCGGC
GATCGAACGGCTGGAGGGCTTCAAGGCCGGGATTTCCACCAACCCCAAGGTGAAGATCATCGCGGTCCAGCCGGTCGACT
GGATGCAGGACAAGGCGGTGGAAGTGTTTTCCGCGATGCTGCAGGCCCATCCGGACATCAAGCTGGTCTATACCAGCAAC
GATCTGGCCGCGGCAGGCGCCTATATCGCGGTGAAGCAGGCCGGCAAGCTCGGCCAGGTCAAGATCATCGGGACCGACGG
ACTGCCGGGACCATCCGGCGGCATCCGCGCGGTGGCCGAGGGTCAATGGACGGCGACCTACGTGTACCCCACCGGTGCCG
CCGAAGCCCTGGACCTGGCCAAGAAGATCCTGATCGACTGCGCATCGTCCGTCCCGCGCCTGGTCACGGTGCCGACGCAG
CGCATCGATGCGAGCAACGCCAAGGAACTCTACGCCAAGGGGGAATGA

Upstream 100 bases:

>100_bases
ACGACGTCAGTGCCGCGATGCAGCTTCTGGCAACCGGCCTGATCATCGTCGGCGCGGCCGGACTGCAGACGTTCGTCGGC
GCCGGAAACGGCACGCGCGC

Downstream 100 bases:

>100_bases
CCGATTGCTCTAACTGTTTGGTCCCGGGATTTGGTGGTGCATTATTTTCACATGAGTGGAAGGATGCGCTATGGGCCAGG
TTCACCACGGAAGCGCCACG

Product: D-ribose-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKSSVETKNKNKKDKTMIVRIAPVVGLALLFPLAAAAAPLAKECGPNKSYVIGFSQANFKEPYRAHVDHELQRLVKNYPQ
FKLVIADGQANDNTQVSQVENFLTQQVDILFVSAFEAAPLTPAVSAVYDKGIPVIELDRRTTGEKFTSFVSGDNRAIAHE
AGLYAASLLPDGGDVAILEGLPSSSPAIERLEGFKAGISTNPKVKIIAVQPVDWMQDKAVEVFSAMLQAHPDIKLVYTSN
DLAAAGAYIAVKQAGKLGQVKIIGTDGLPGPSGGIRAVAEGQWTATYVYPTGAAEALDLAKKILIDCASSVPRLVTVPTQ
RIDASNAKELYAKGE

Sequences:

>Translated_335_residues
MKSSVETKNKNKKDKTMIVRIAPVVGLALLFPLAAAAAPLAKECGPNKSYVIGFSQANFKEPYRAHVDHELQRLVKNYPQ
FKLVIADGQANDNTQVSQVENFLTQQVDILFVSAFEAAPLTPAVSAVYDKGIPVIELDRRTTGEKFTSFVSGDNRAIAHE
AGLYAASLLPDGGDVAILEGLPSSSPAIERLEGFKAGISTNPKVKIIAVQPVDWMQDKAVEVFSAMLQAHPDIKLVYTSN
DLAAAGAYIAVKQAGKLGQVKIIGTDGLPGPSGGIRAVAEGQWTATYVYPTGAAEALDLAKKILIDCASSVPRLVTVPTQ
RIDASNAKELYAKGE
>Mature_335_residues
MKSSVETKNKNKKDKTMIVRIAPVVGLALLFPLAAAAAPLAKECGPNKSYVIGFSQANFKEPYRAHVDHELQRLVKNYPQ
FKLVIADGQANDNTQVSQVENFLTQQVDILFVSAFEAAPLTPAVSAVYDKGIPVIELDRRTTGEKFTSFVSGDNRAIAHE
AGLYAASLLPDGGDVAILEGLPSSSPAIERLEGFKAGISTNPKVKIIAVQPVDWMQDKAVEVFSAMLQAHPDIKLVYTSN
DLAAAGAYIAVKQAGKLGQVKIIGTDGLPGPSGGIRAVAEGQWTATYVYPTGAAEALDLAKKILIDCASSVPRLVTVPTQ
RIDASNAKELYAKGE

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=206, Percent_Identity=33.495145631068, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790526, Length=201, Percent_Identity=31.8407960199005, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790674, Length=279, Percent_Identity=29.3906810035842, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789990, Length=241, Percent_Identity=26.9709543568465, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35704; Mature: 35704

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSVETKNKNKKDKTMIVRIAPVVGLALLFPLAAAAAPLAKECGPNKSYVIGFSQANFK
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
EPYRAHVDHELQRLVKNYPQFKLVIADGQANDNTQVSQVENFLTQQVDILFVSAFEAAPL
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCC
TPAVSAVYDKGIPVIELDRRTTGEKFTSFVSGDNRAIAHEAGLYAASLLPDGGDVAILEG
CHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEHHCCCEEEEECCCCCCEEEEEC
LPSSSPAIERLEGFKAGISTNPKVKIIAVQPVDWMQDKAVEVFSAMLQAHPDIKLVYTSN
CCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DLAAAGAYIAVKQAGKLGQVKIIGTDGLPGPSGGIRAVAEGQWTATYVYPTGAAEALDLA
CHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHH
KKILIDCASSVPRLVTVPTQRIDASNAKELYAKGE
HHHHHHHHHCCCEEEECCCHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MKSSVETKNKNKKDKTMIVRIAPVVGLALLFPLAAAAAPLAKECGPNKSYVIGFSQANFK
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
EPYRAHVDHELQRLVKNYPQFKLVIADGQANDNTQVSQVENFLTQQVDILFVSAFEAAPL
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCC
TPAVSAVYDKGIPVIELDRRTTGEKFTSFVSGDNRAIAHEAGLYAASLLPDGGDVAILEG
CHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEHHCCCEEEEECCCCCCEEEEEC
LPSSSPAIERLEGFKAGISTNPKVKIIAVQPVDWMQDKAVEVFSAMLQAHPDIKLVYTSN
CCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DLAAAGAYIAVKQAGKLGQVKIIGTDGLPGPSGGIRAVAEGQWTATYVYPTGAAEALDLA
CHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHH
KKILIDCASSVPRLVTVPTQRIDASNAKELYAKGE
HHHHHHHHHCCCEEEECCCHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]