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The map label for this gene is priA [H]

Identifier: 162148141

GI number: 162148141

Start: 2428728

End: 2431040

Strand: Direct

Name: priA [H]

Synonym: GDI_2358

Alternate gene names: 162148141

Gene position: 2428728-2431040 (Clockwise)

Preceding gene: 162148136

Following gene: 162148144

Centisome position: 61.58

GC content: 73.32

Gene sequence:

>2313_bases
GTGTGTTTCCGAGCACCGAAGCGGAGCGGCCTTATGGCCGTGAGCATCGGAGCGCAGGAAATGCGCGTCGGACCGCCAGC
AGCGGGGCTTTTGAAGCAGCCTCCGACCGGCCGGCCGCGTGTCGCGGTCCTGCTGCCTCTGCCCTTCCCCGGCCCGTTCG
ACTATGCCGCACCGCCGGACCTGCCCGCCCCCCCGCTGCAACCCGGCGACATCGTCACCGTGCCGCTGGGGCGGCGTACC
GAAACCGGCGTGGTCTGGGACCCGGAGCGCAACCTGCCGCCCGAATTCACCCCGCCCCCCATGAAGAGCGTGGCGGACGG
ACGGCTGAAGCCCGTCATCGCGCGGCTGGACCAGCCGCCCTTGCCGGCCTCGCTGCGCCGCTTCGTCGACTGGGTCGCGG
CCTATACGCTTTCCCCCCCGGGGCTGGTGCTGGCCATGGCGCTGCGCACCGGCACCGCCGCCCAGGCCGCCCCCGCGCTG
GGCTGGGCCGTTGCCGATCCGCCGCCGGGCCTGCGCGACACCCCGGCACGGCGAAGGGTGCTGGACATCGCCGCGCATGA
CGGGCCGCTGACCGCCGCCGAACTGGCCCGCCGTGCCGGGGTCGGCACCAGCGTGGTGCGCGGCCTGGCGGATGCCGGGG
CGCTGCGCGGGGTAACGATGACGCCGCGCCCCGCCTTCGTGCGGCCCGATCCGGACCATTGCCCCCCGGCACTGGACGGC
GAGCAGGCGGAATCGGCCCGGGCCCTGCGTGCCCTGGCGGCGGAGGGCGGCTTTTCCGTCACCCTGCTGGAAGGAGTGAC
CGGATCGGGCAAGACCGAGATCTACATGGAGGCCATTGCCGCCTGCCTGGCGCGCGGGAAGCAGGTGATGGTGCTGCTGC
CGGAAATCGCCCTGTCCTCGCAATGGACCGGGCGTTTCGCCCGCCGCTTCGGGGCGGAGCCCGCGCTGTGGCATTCCGAC
CTGGGGCCGCGCCTGCGCCGCGTCACCTGGCACGCGGTGGCCGACGGCACGGCGCGGGTGGTGGTGGGCGCGCGCTCGGC
CCTGTTCCTGCCCTTCGCGGAACTGGGGCTGATCATCGTGGACGAGGAACACGAGACCGCGTTCAAGCAGGAAGACGGCG
TGACCTACCACGCGCGCGACATGGCCGTGGTGCGGGCGCGCATGGACCGCGCGCCGGTCGTCCTGGTCTCGGCCACGCCC
AGCCTGGAGACCCTGGCCAATGTCGGGGCCGGCCGCTACCGCCATCTGGTGCTGACCGCGCGCCATGGCGGGGCGACGAT
GCCCGAGGTGCGCACCATCGACATGCGCGCCCATCCGCCCGAACGGGGACTGTTCCTCTCGCCGGTGCTGACCGAGGCCA
TCCGGCAGGCGATCGGGCGCGGCGAGCAGGGGATGCTGTTCCTCAACCGGCGGGGCTATGCGCCGCTGACCCTGTGCCGT
ACCTGCGGCCACCGCCTGCAATGCCCGAACTGCACCGCCTGGCTGGTGGAACATCGCGCGCGGCAGATCCTGACCTGCCA
TCATTGCGGCTATCAGGAAGCGACACCGCCCGCCTGCCCCGAATGCCACGCCGAATCCAGCCTGACGCCGATCGGCCCCG
GCGTGGAGCGCATTACCGAGGAAGCGCGCGCCACCTTCCCCGATGCGCGCATCCTGGTGATGGCCAGCGACACCCTGCCC
GGCCCCGCCGCCACCGCCGACGCCGTGGCGCGGATCGCGCGGCGCGAGATCGACCTGGTGATCGGCACGCAGATCGTGGC
CAAGGGCTGGCATTTTCCGCATCTGACGCTGGTCGGTGTCGTCGATGCCGACCTGGGCCTGGGCGGCGGCGACCTGCGCG
CGGCGGAACGCACGGTGCAGTTGCTGCATCAGGTCGCCGGCCGCGCCGGCCGTGCCGAGGCCCCGGGGACGGTGCTGCTG
CAGAGCTACACCCCCGACCATCCGGTGATGCTGGCGCTGGTGTCAGGCGATTTCGAGAGTTTCATGCGCCAGGAGGCCGA
GCAGCGCCGCCCCGGCTTCTGGCCCCCCTTCGGCCGGCTGGCCGCCCTGATCGTCAGCGCGGATACGCCGGAGGCCGCCG
ACGCCACCGCCCGGGCCCTGGGTCAGGCAGCGCCCCGGGGCGACAGCCTGCAGGTGCTGGGCCCGGCCCCCGCGCCGCTG
GCCATCCTGCGCGGCCGCCACCGCAGGCGACTGCTGCTGCGCACGCGGCGCGAGGTCGCGGTCCAGCCCCTGCTGCGGCA
ATGGCTGGACCGCGTGACGCCCGAACGCGGGGCCCGGATCGACATCGATATCGATCCGGTCTCGTTCCTTTAG

Upstream 100 bases:

>100_bases
CGAGTCTAGACAAACGCACCCCGGCACGGAAGGCGGGAATGGGAGGCTGCTTCAAAAGCCCCGCTGCTGAGCGAGAGCGG
CCCGAAGGGTCGCGCCCGTC

Downstream 100 bases:

>100_bases
CAGGCCCTATACCGCGTGGACGTTCGCCTCGCGCTCCCAGAACCGCAGAGCCCTGCACGCGGCGATGAAGGCGGTCGCGT
CGGCGGGCGTGGACAGTTTA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MCFRAPKRSGLMAVSIGAQEMRVGPPAAGLLKQPPTGRPRVAVLLPLPFPGPFDYAAPPDLPAPPLQPGDIVTVPLGRRT
ETGVVWDPERNLPPEFTPPPMKSVADGRLKPVIARLDQPPLPASLRRFVDWVAAYTLSPPGLVLAMALRTGTAAQAAPAL
GWAVADPPPGLRDTPARRRVLDIAAHDGPLTAAELARRAGVGTSVVRGLADAGALRGVTMTPRPAFVRPDPDHCPPALDG
EQAESARALRALAAEGGFSVTLLEGVTGSGKTEIYMEAIAACLARGKQVMVLLPEIALSSQWTGRFARRFGAEPALWHSD
LGPRLRRVTWHAVADGTARVVVGARSALFLPFAELGLIIVDEEHETAFKQEDGVTYHARDMAVVRARMDRAPVVLVSATP
SLETLANVGAGRYRHLVLTARHGGATMPEVRTIDMRAHPPERGLFLSPVLTEAIRQAIGRGEQGMLFLNRRGYAPLTLCR
TCGHRLQCPNCTAWLVEHRARQILTCHHCGYQEATPPACPECHAESSLTPIGPGVERITEEARATFPDARILVMASDTLP
GPAATADAVARIARREIDLVIGTQIVAKGWHFPHLTLVGVVDADLGLGGGDLRAAERTVQLLHQVAGRAGRAEAPGTVLL
QSYTPDHPVMLALVSGDFESFMRQEAEQRRPGFWPPFGRLAALIVSADTPEAADATARALGQAAPRGDSLQVLGPAPAPL
AILRGRHRRRLLLRTRREVAVQPLLRQWLDRVTPERGARIDIDIDPVSFL

Sequences:

>Translated_770_residues
MCFRAPKRSGLMAVSIGAQEMRVGPPAAGLLKQPPTGRPRVAVLLPLPFPGPFDYAAPPDLPAPPLQPGDIVTVPLGRRT
ETGVVWDPERNLPPEFTPPPMKSVADGRLKPVIARLDQPPLPASLRRFVDWVAAYTLSPPGLVLAMALRTGTAAQAAPAL
GWAVADPPPGLRDTPARRRVLDIAAHDGPLTAAELARRAGVGTSVVRGLADAGALRGVTMTPRPAFVRPDPDHCPPALDG
EQAESARALRALAAEGGFSVTLLEGVTGSGKTEIYMEAIAACLARGKQVMVLLPEIALSSQWTGRFARRFGAEPALWHSD
LGPRLRRVTWHAVADGTARVVVGARSALFLPFAELGLIIVDEEHETAFKQEDGVTYHARDMAVVRARMDRAPVVLVSATP
SLETLANVGAGRYRHLVLTARHGGATMPEVRTIDMRAHPPERGLFLSPVLTEAIRQAIGRGEQGMLFLNRRGYAPLTLCR
TCGHRLQCPNCTAWLVEHRARQILTCHHCGYQEATPPACPECHAESSLTPIGPGVERITEEARATFPDARILVMASDTLP
GPAATADAVARIARREIDLVIGTQIVAKGWHFPHLTLVGVVDADLGLGGGDLRAAERTVQLLHQVAGRAGRAEAPGTVLL
QSYTPDHPVMLALVSGDFESFMRQEAEQRRPGFWPPFGRLAALIVSADTPEAADATARALGQAAPRGDSLQVLGPAPAPL
AILRGRHRRRLLLRTRREVAVQPLLRQWLDRVTPERGARIDIDIDPVSFL
>Mature_770_residues
MCFRAPKRSGLMAVSIGAQEMRVGPPAAGLLKQPPTGRPRVAVLLPLPFPGPFDYAAPPDLPAPPLQPGDIVTVPLGRRT
ETGVVWDPERNLPPEFTPPPMKSVADGRLKPVIARLDQPPLPASLRRFVDWVAAYTLSPPGLVLAMALRTGTAAQAAPAL
GWAVADPPPGLRDTPARRRVLDIAAHDGPLTAAELARRAGVGTSVVRGLADAGALRGVTMTPRPAFVRPDPDHCPPALDG
EQAESARALRALAAEGGFSVTLLEGVTGSGKTEIYMEAIAACLARGKQVMVLLPEIALSSQWTGRFARRFGAEPALWHSD
LGPRLRRVTWHAVADGTARVVVGARSALFLPFAELGLIIVDEEHETAFKQEDGVTYHARDMAVVRARMDRAPVVLVSATP
SLETLANVGAGRYRHLVLTARHGGATMPEVRTIDMRAHPPERGLFLSPVLTEAIRQAIGRGEQGMLFLNRRGYAPLTLCR
TCGHRLQCPNCTAWLVEHRARQILTCHHCGYQEATPPACPECHAESSLTPIGPGVERITEEARATFPDARILVMASDTLP
GPAATADAVARIARREIDLVIGTQIVAKGWHFPHLTLVGVVDADLGLGGGDLRAAERTVQLLHQVAGRAGRAEAPGTVLL
QSYTPDHPVMLALVSGDFESFMRQEAEQRRPGFWPPFGRLAALIVSADTPEAADATARALGQAAPRGDSLQVLGPAPAPL
AILRGRHRRRLLLRTRREVAVQPLLRQWLDRVTPERGARIDIDIDPVSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=761, Percent_Identity=35.4796320630749, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 82695; Mature: 82695

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFRAPKRSGLMAVSIGAQEMRVGPPAAGLLKQPPTGRPRVAVLLPLPFPGPFDYAAPPD
CCCCCCCCCCEEEEEECHHHHCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
LPAPPLQPGDIVTVPLGRRTETGVVWDPERNLPPEFTPPPMKSVADGRLKPVIARLDQPP
CCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCC
LPASLRRFVDWVAAYTLSPPGLVLAMALRTGTAAQAAPALGWAVADPPPGLRDTPARRRV
CCHHHHHHHHHHHHHCCCCCHHEEHHHHHCCCCHHHCCCCCEEECCCCCCCCCCHHHHHE
LDIAAHDGPLTAAELARRAGVGTSVVRGLADAGALRGVTMTPRPAFVRPDPDHCPPALDG
EEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCEECCCCCCCCCCCCC
EQAESARALRALAAEGGFSVTLLEGVTGSGKTEIYMEAIAACLARGKQVMVLLPEIALSS
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHCC
QWTGRFARRFGAEPALWHSDLGPRLRRVTWHAVADGTARVVVGARSALFLPFAELGLIIV
CHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEECCCCEEEEEECCCHHCCCHHHCCEEEE
DEEHETAFKQEDGVTYHARDMAVVRARMDRAPVVLVSATPSLETLANVGAGRYRHLVLTA
ECCHHHHHHHCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEEE
RHGGATMPEVRTIDMRAHPPERGLFLSPVLTEAIRQAIGRGEQGMLFLNRRGYAPLTLCR
CCCCCCCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
TCGHRLQCPNCTAWLVEHRARQILTCHHCGYQEATPPACPECHAESSLTPIGPGVERITE
HCCCCCCCCCHHHHHHHHHHHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
EARATFPDARILVMASDTLPGPAATADAVARIARREIDLVIGTQIVAKGWHFPHLTLVGV
HHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHCCCCCCCEEEEEE
VDADLGLGGGDLRAAERTVQLLHQVAGRAGRAEAPGTVLLQSYTPDHPVMLALVSGDFES
EECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHH
FMRQEAEQRRPGFWPPFGRLAALIVSADTPEAADATARALGQAAPRGDSLQVLGPAPAPL
HHHHHHHHCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCH
AILRGRHRRRLLLRTRREVAVQPLLRQWLDRVTPERGARIDIDIDPVSFL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MCFRAPKRSGLMAVSIGAQEMRVGPPAAGLLKQPPTGRPRVAVLLPLPFPGPFDYAAPPD
CCCCCCCCCCEEEEEECHHHHCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
LPAPPLQPGDIVTVPLGRRTETGVVWDPERNLPPEFTPPPMKSVADGRLKPVIARLDQPP
CCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCC
LPASLRRFVDWVAAYTLSPPGLVLAMALRTGTAAQAAPALGWAVADPPPGLRDTPARRRV
CCHHHHHHHHHHHHHCCCCCHHEEHHHHHCCCCHHHCCCCCEEECCCCCCCCCCHHHHHE
LDIAAHDGPLTAAELARRAGVGTSVVRGLADAGALRGVTMTPRPAFVRPDPDHCPPALDG
EEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCEECCCCCCCCCCCCC
EQAESARALRALAAEGGFSVTLLEGVTGSGKTEIYMEAIAACLARGKQVMVLLPEIALSS
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHCC
QWTGRFARRFGAEPALWHSDLGPRLRRVTWHAVADGTARVVVGARSALFLPFAELGLIIV
CHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEECCCCEEEEEECCCHHCCCHHHCCEEEE
DEEHETAFKQEDGVTYHARDMAVVRARMDRAPVVLVSATPSLETLANVGAGRYRHLVLTA
ECCHHHHHHHCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEEE
RHGGATMPEVRTIDMRAHPPERGLFLSPVLTEAIRQAIGRGEQGMLFLNRRGYAPLTLCR
CCCCCCCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
TCGHRLQCPNCTAWLVEHRARQILTCHHCGYQEATPPACPECHAESSLTPIGPGVERITE
HCCCCCCCCCHHHHHHHHHHHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
EARATFPDARILVMASDTLPGPAATADAVARIARREIDLVIGTQIVAKGWHFPHLTLVGV
HHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHCCCCCCCEEEEEE
VDADLGLGGGDLRAAERTVQLLHQVAGRAGRAEAPGTVLLQSYTPDHPVMLALVSGDFES
EECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHH
FMRQEAEQRRPGFWPPFGRLAALIVSADTPEAADATARALGQAAPRGDSLQVLGPAPAPL
HHHHHHHHCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCH
AILRGRHRRRLLLRTRREVAVQPLLRQWLDRVTPERGARIDIDIDPVSFL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]