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The map label for this gene is leuA [H]

Identifier: 162148122

GI number: 162148122

Start: 2406948

End: 2408522

Strand: Reverse

Name: leuA [H]

Synonym: GDI_2339

Alternate gene names: 162148122

Gene position: 2408522-2406948 (Counterclockwise)

Preceding gene: 162148123

Following gene: 162148120

Centisome position: 61.07

GC content: 67.56

Gene sequence:

>1575_bases
ATGACTATCGACCATCCGTCCTTCGGCCGCATCGACAATGATCGTGTCATCATTTTCGACACGACCCTGCGTGACGGCGA
ACAATCGCCCGGCTTCTCGATGAACCTGGCCGAAAAGCTCCGCATGGCCGAGGCGCTGGCGGAACTGGGCGTCGATGTGA
TCGAGGCGGGATTCCCCGTGGCGTCGAAGGGCGATTTCGACAGCGTGCACCAGATAGCCTCGCACATTCGTGCCCCCGTG
ATCTGCGCCCTGGCCCGCAGCGGCGGGCGGAACGACATCGCCAGCGCCGGCGAGGCCATTCGCCCGGCCGCGCGGGGACG
CATCCACAACTTCATCTCCACCTCGCCGCTGCACATGAAATACAAGCTGCGGATGGAGCCCGAGACGGTGCTGGAGCTGA
TCACCGCCGGCAACCAGGCGGCGCGGCAGTTCACCGACGACGTGGAATGGTCGGCCGAGGACGGGTCGCGCACCGACCCG
GATTTCCTGTGCCGGTGCGTCGAGGCCGCGATCAAGGCCGGCGCCACCACGATCAACATCCCCGATACCGTCGGCTATGC
GACGCCCGAGGACATGGCCCGCATCTTCGCCGACCTGCTGGCCCGCGTGCCGGGAGCGGATACCGTGATCTTCTCGGCCC
ATAACCACAACGACCTGGGGCTGGGGGTGGCCAACACCATCGCCTCGCTGTCCGCCGGCGCCCGGCAGATCGAATGCACG
ATCAACGGCATCGGCGAGCGCGCGGGCAACGCGGCGCTGGAAGAGATCGTCATGGCGCTGCGCACCCGCCAGGACGTGCT
GCCGTACAGCACCGGCATCAAGACGCAGAACCTGCTGCGGACGTCGCGGATGCTGGCGACGATCACCGGGTTCGACGTCC
AGCCCAACAAGGCGATCGTCGGCCGCAACGCCTTCGCGCATGAAAGCGGCATCCACCAGGACGGCGTGCTGAAGAATGCC
GCGACCTACGAGATCATGACGCCCGAGAGCGTGGGCTGGACCCGGTCGTCGCTGGTCCTGGGCAAGCATTCCGGCCGCGC
CGCCTTTCGCGACAAGCTGAAGGCCATGGGATACGGCGAGATGGACGAGGCGCATCTGAATGATGCGTTCGCGCGGTTCA
AGGACCTGGCCGACCGCAAGAAGGTCGTCTACGACGAGGACCTGGTCGCCCTGGTCGATGACGAGGCCCGCGACCATGCC
CGCATCCGCTTCGTCTCGCTGGATATCGAGGCCGGGTCTCGCCGTCCGGCGCGCGCCGACCTGGCGCTGGAGGTCGACGG
CGAGGCGCGCAGCGCCACCGTGACTGGCCAGGGGCCCGTCGATGCGGCGTTCAACGCGGTCCGCGAGATCTTCCCGCACG
AGGCGACGCTGCAGCTCTATTCGGTCGGCGCGGTGACCGAGGGCAGCGACGCGCAGGCGCGCACCACCGTGCGGCTGGAG
GAAGATGGCAAGCTGGTGGACGGCCAGGGCGCGGACAGCGACACGCTGGTGTCGGCCGTGCGGGCCTACGTTCATGCGCT
GAACAAGCTGCTGGTCAAGCGTACCCGCGCCGAACCCGAAGCCATGACGGCCTGA

Upstream 100 bases:

>100_bases
CGGTACGCCCAGGGGAAGTTGCGGGAGCCATCCCGCATGCAAGGCAGGAACGCCCGCACGGCGTTCAAAGGCGAACATCC
GGATCAATCCGAGGTAATTC

Downstream 100 bases:

>100_bases
CCGGCACCGGACGGCCGGTATCATGACGGGCGGGGCATCCTTCGGGTGCCCCGCTTTTTTTCGTCGGTCCGTCCTCAATA
ACCGTTGTATAACCGCCCGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTIDHPSFGRIDNDRVIIFDTTLRDGEQSPGFSMNLAEKLRMAEALAELGVDVIEAGFPVASKGDFDSVHQIASHIRAPV
ICALARSGGRNDIASAGEAIRPAARGRIHNFISTSPLHMKYKLRMEPETVLELITAGNQAARQFTDDVEWSAEDGSRTDP
DFLCRCVEAAIKAGATTINIPDTVGYATPEDMARIFADLLARVPGADTVIFSAHNHNDLGLGVANTIASLSAGARQIECT
INGIGERAGNAALEEIVMALRTRQDVLPYSTGIKTQNLLRTSRMLATITGFDVQPNKAIVGRNAFAHESGIHQDGVLKNA
ATYEIMTPESVGWTRSSLVLGKHSGRAAFRDKLKAMGYGEMDEAHLNDAFARFKDLADRKKVVYDEDLVALVDDEARDHA
RIRFVSLDIEAGSRRPARADLALEVDGEARSATVTGQGPVDAAFNAVREIFPHEATLQLYSVGAVTEGSDAQARTTVRLE
EDGKLVDGQGADSDTLVSAVRAYVHALNKLLVKRTRAEPEAMTA

Sequences:

>Translated_524_residues
MTIDHPSFGRIDNDRVIIFDTTLRDGEQSPGFSMNLAEKLRMAEALAELGVDVIEAGFPVASKGDFDSVHQIASHIRAPV
ICALARSGGRNDIASAGEAIRPAARGRIHNFISTSPLHMKYKLRMEPETVLELITAGNQAARQFTDDVEWSAEDGSRTDP
DFLCRCVEAAIKAGATTINIPDTVGYATPEDMARIFADLLARVPGADTVIFSAHNHNDLGLGVANTIASLSAGARQIECT
INGIGERAGNAALEEIVMALRTRQDVLPYSTGIKTQNLLRTSRMLATITGFDVQPNKAIVGRNAFAHESGIHQDGVLKNA
ATYEIMTPESVGWTRSSLVLGKHSGRAAFRDKLKAMGYGEMDEAHLNDAFARFKDLADRKKVVYDEDLVALVDDEARDHA
RIRFVSLDIEAGSRRPARADLALEVDGEARSATVTGQGPVDAAFNAVREIFPHEATLQLYSVGAVTEGSDAQARTTVRLE
EDGKLVDGQGADSDTLVSAVRAYVHALNKLLVKRTRAEPEAMTA
>Mature_523_residues
TIDHPSFGRIDNDRVIIFDTTLRDGEQSPGFSMNLAEKLRMAEALAELGVDVIEAGFPVASKGDFDSVHQIASHIRAPVI
CALARSGGRNDIASAGEAIRPAARGRIHNFISTSPLHMKYKLRMEPETVLELITAGNQAARQFTDDVEWSAEDGSRTDPD
FLCRCVEAAIKAGATTINIPDTVGYATPEDMARIFADLLARVPGADTVIFSAHNHNDLGLGVANTIASLSAGARQIECTI
NGIGERAGNAALEEIVMALRTRQDVLPYSTGIKTQNLLRTSRMLATITGFDVQPNKAIVGRNAFAHESGIHQDGVLKNAA
TYEIMTPESVGWTRSSLVLGKHSGRAAFRDKLKAMGYGEMDEAHLNDAFARFKDLADRKKVVYDEDLVALVDDEARDHAR
IRFVSLDIEAGSRRPARADLALEVDGEARSATVTGQGPVDAAFNAVREIFPHEATLQLYSVGAVTEGSDAQARTTVRLEE
DGKLVDGQGADSDTLVSAVRAYVHALNKLLVKRTRAEPEAMTA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=500, Percent_Identity=46.4, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320019, Length=386, Percent_Identity=25.6476683937824, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=26.1780104712042, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=551, Percent_Identity=23.7749546279492, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324225, Length=544, Percent_Identity=23.8970588235294, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56559; Mature: 56428

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDHPSFGRIDNDRVIIFDTTLRDGEQSPGFSMNLAEKLRMAEALAELGVDVIEAGFPV
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCC
ASKGDFDSVHQIASHIRAPVICALARSGGRNDIASAGEAIRPAARGRIHNFISTSPLHMK
CCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCHHHCHHHHHHHHHHHCCCCCEEE
YKLRMEPETVLELITAGNQAARQFTDDVEWSAEDGSRTDPDFLCRCVEAAIKAGATTINI
EEEECCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
PDTVGYATPEDMARIFADLLARVPGADTVIFSAHNHNDLGLGVANTIASLSAGARQIECT
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEE
INGIGERAGNAALEEIVMALRTRQDVLPYSTGIKTQNLLRTSRMLATITGFDVQPNKAIV
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEE
GRNAFAHESGIHQDGVLKNAATYEIMTPESVGWTRSSLVLGKHSGRAAFRDKLKAMGYGE
CCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEEECCCCCHHHHHHHHHCCCCC
MDEAHLNDAFARFKDLADRKKVVYDEDLVALVDDEARDHARIRFVSLDIEAGSRRPARAD
CCHHHHHHHHHHHHHHHHHHCEEECCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCC
LALEVDGEARSATVTGQGPVDAAFNAVREIFPHEATLQLYSVGAVTEGSDAQARTTVRLE
EEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEEC
EDGKLVDGQGADSDTLVSAVRAYVHALNKLLVKRTRAEPEAMTA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
TIDHPSFGRIDNDRVIIFDTTLRDGEQSPGFSMNLAEKLRMAEALAELGVDVIEAGFPV
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCC
ASKGDFDSVHQIASHIRAPVICALARSGGRNDIASAGEAIRPAARGRIHNFISTSPLHMK
CCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCHHHCHHHHHHHHHHHCCCCCEEE
YKLRMEPETVLELITAGNQAARQFTDDVEWSAEDGSRTDPDFLCRCVEAAIKAGATTINI
EEEECCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
PDTVGYATPEDMARIFADLLARVPGADTVIFSAHNHNDLGLGVANTIASLSAGARQIECT
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEE
INGIGERAGNAALEEIVMALRTRQDVLPYSTGIKTQNLLRTSRMLATITGFDVQPNKAIV
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEE
GRNAFAHESGIHQDGVLKNAATYEIMTPESVGWTRSSLVLGKHSGRAAFRDKLKAMGYGE
CCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEEECCCCCHHHHHHHHHCCCCC
MDEAHLNDAFARFKDLADRKKVVYDEDLVALVDDEARDHARIRFVSLDIEAGSRRPARAD
CCHHHHHHHHHHHHHHHHHHCEEECCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCC
LALEVDGEARSATVTGQGPVDAAFNAVREIFPHEATLQLYSVGAVTEGSDAQARTTVRLE
EEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEEC
EDGKLVDGQGADSDTLVSAVRAYVHALNKLLVKRTRAEPEAMTA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA