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The map label for this gene is rpoH [H]

Identifier: 162148038

GI number: 162148038

Start: 2311027

End: 2312004

Strand: Direct

Name: rpoH [H]

Synonym: GDI_2254

Alternate gene names: 162148038

Gene position: 2311027-2312004 (Clockwise)

Preceding gene: 162148037

Following gene: 162148043

Centisome position: 58.59

GC content: 62.47

Gene sequence:

>978_bases
ATGCGGGCACGGTCGTGCAACCCTTCATATGGATGTGCCGTAGGACATCCAGGGACAGATGACCACCCCCAGGGAAAAGG
AGTCCGGTCCATGGCCTCTTCCGTTCTCAATGTTGGTCCTGAATCCAACCTGTCAAGATACCTTCAGGACATCCGTAAAT
TTCCGATGCTGTCGCCCGAGGATGAACAGCGCCTGTCCCGGCGCTGGAAGGACAAGGGCGATACCGAGGCCGCGCATGCG
CTCGTCACCTCGCACCTGCGCCTGGTCGCCAAGATCGCCATGGGCTATCGCGGCTATGGCCTGCCGCTGGGCGAGCTGAT
CAGCGAAGGCAATATCGGGATGATGCAGGCGGTCCGCCGCTTCGACCCGGATCGCGGCTTCCGCCTGGCGACCTATGCGA
TGTGGTGGATTCGGGCCGCCATCCAGGAATATATCCTGCATAGCTGGTCGCTGGTGAAGATGGGCACCACCGCCGCGCAG
AAGAAGCTGTTCTTCAACCTGCGCCGCCTGAAGGGCCAGATGCAGGCCATCGACGACGGCGACCTGAAGCCCGAGCAGGT
GAACAAGATCGCCGAATCGCTGGGTGTGCCCGAGCAGGACGTCATCAACATGAACCGCCGCCTGTCGGCGCCGGATCACA
GCCTGAACGCCCCGCTGCGCGCCGACAGCGAAGGCGAATGGCAGGACTGGCTGGTCGACGAACACGACAACCAGGAACAG
ACCCTGGCGGAAAACGAGGAATTCAGCGGACGCAAGGCATTGCTGGACAATGCCATGAAGACGCTGAACGACCGTGAGCG
CCACATCCTGACCGAACGCCGCCTGAAGGACGACCCGGCCACGCTGGAAGAATTGTCGCACACCTACAACATCTCGCGCG
AGCGGGTGCGGCAGATCGAGGTCCGGGCGTTCGAGAAGGTGCAGGCGGCGATGAAGGCGGAAGTCGAGGCCCATCGCGAA
GCCCACGCCGCAAACTGA

Upstream 100 bases:

>100_bases
CATAACTCCGACGGGTGACGCACCGCCGGATTGGACGTCCCGCGTCGACGCGGCCCATGGAGGGGGCGTCTCGCGGGGAC
GTGAAGTCCATCCACGCGTC

Downstream 100 bases:

>100_bases
GAATGACGGGGGGCGTTGAAGCGCCTCCCCCTCTTCCGTTTCCCGTTTCCGGGTGGATCGTCAGTTCTCGAACGGGTCGC
GCATCAGGATCGTATCGTCG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRARSCNPSYGCAVGHPGTDDHPQGKGVRSMASSVLNVGPESNLSRYLQDIRKFPMLSPEDEQRLSRRWKDKGDTEAAHA
LVTSHLRLVAKIAMGYRGYGLPLGELISEGNIGMMQAVRRFDPDRGFRLATYAMWWIRAAIQEYILHSWSLVKMGTTAAQ
KKLFFNLRRLKGQMQAIDDGDLKPEQVNKIAESLGVPEQDVINMNRRLSAPDHSLNAPLRADSEGEWQDWLVDEHDNQEQ
TLAENEEFSGRKALLDNAMKTLNDRERHILTERRLKDDPATLEELSHTYNISRERVRQIEVRAFEKVQAAMKAEVEAHRE
AHAAN

Sequences:

>Translated_325_residues
MRARSCNPSYGCAVGHPGTDDHPQGKGVRSMASSVLNVGPESNLSRYLQDIRKFPMLSPEDEQRLSRRWKDKGDTEAAHA
LVTSHLRLVAKIAMGYRGYGLPLGELISEGNIGMMQAVRRFDPDRGFRLATYAMWWIRAAIQEYILHSWSLVKMGTTAAQ
KKLFFNLRRLKGQMQAIDDGDLKPEQVNKIAESLGVPEQDVINMNRRLSAPDHSLNAPLRADSEGEWQDWLVDEHDNQEQ
TLAENEEFSGRKALLDNAMKTLNDRERHILTERRLKDDPATLEELSHTYNISRERVRQIEVRAFEKVQAAMKAEVEAHRE
AHAAN
>Mature_325_residues
MRARSCNPSYGCAVGHPGTDDHPQGKGVRSMASSVLNVGPESNLSRYLQDIRKFPMLSPEDEQRLSRRWKDKGDTEAAHA
LVTSHLRLVAKIAMGYRGYGLPLGELISEGNIGMMQAVRRFDPDRGFRLATYAMWWIRAAIQEYILHSWSLVKMGTTAAQ
KKLFFNLRRLKGQMQAIDDGDLKPEQVNKIAESLGVPEQDVINMNRRLSAPDHSLNAPLRADSEGEWQDWLVDEHDNQEQ
TLAENEEFSGRKALLDNAMKTLNDRERHILTERRLKDDPATLEELSHTYNISRERVRQIEVRAFEKVQAAMKAEVEAHRE
AHAAN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=293, Percent_Identity=39.9317406143345, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789098, Length=270, Percent_Identity=34.4444444444444, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789448, Length=273, Percent_Identity=30.7692307692308, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36967; Mature: 36967

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00716 SIGMA70_2 ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]