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The map label for this gene is mazG [H]

Identifier: 162148043

GI number: 162148043

Start: 2317011

End: 2317802

Strand: Direct

Name: mazG [H]

Synonym: GDI_2259

Alternate gene names: 162148043

Gene position: 2317011-2317802 (Clockwise)

Preceding gene: 162148038

Following gene: 162148053

Centisome position: 58.75

GC content: 68.31

Gene sequence:

>792_bases
ATGGAGTTCCCCCCGATGTCCGAAGCCACCCAGGCCCTGTCCCGCCTGATAGACATCATGGCAAGGCTGCGCGATCCGGT
GGCGGGATGTCCGTGGGACCGCGAGCAGGACTTCGCCACGATCGCCCCGTTCGCGATCGAGGAAGCCTATGAGGTCGAGG
ACACGATCGCCCGGCAGGATTGGGCGGCGCTGCCGGACGAACTGGGCGACCTGTTGCTGCAGGTCGTCTATCACGCCCGC
ATGGCGGAGGAAGCCGGCTGGTTCGATTTCGCGACCGTGGCCGGCATGATCGGCGACAAGATGGTCCGCCGCCATCCCCA
TGTGTTCGGCGATGCCACGCTGCAGGCCGGCCAGTGGGAAGATGAAAAGGAACGCGAGCGGCAGGCCCGGCGGGAGGAGG
GTACGCTGGCCGGCATTCCGGCGGCGCTGCCGGCCCTGCTGCGCGCGCGCAAATTGTCGGCGCGGGCGGCCCGGGTCGGA
TTCGACTGGCCCGATGCAAGGGACGTCCTGGACAAGGTGGACGAGGAAGTCGCGGAACTGAAGGCCGAACTGGATTCGGG
CGATCGCGCGCGCATCACCGACGAACTGGGGGACGTGCTGTTCACCCTGGCGACGCTGGCCCGCAAACTGGACATCGACC
CGGAAGCCTGCCTGCGCCAGGCCAATGCCAAATTCACCCGCCGCTTCACCGCCATGGAACAGGACCTGGCCCGGCGGGGC
ACCAGCCTGCCGGACAGCACGCTGGCCGAGATGGAAGCCGGCTGGCAGGAGGTCAAGCGGCGCGAGCGGTAG

Upstream 100 bases:

>100_bases
GATAGGGGCCTGTACCATAATCCAAAGGCCGATACCCCTGGAGACGGATTCTTTGTCAATGTCCCGGGGCGCAGGGCATC
ATGCCCGGCCTTTTCATAAC

Downstream 100 bases:

>100_bases
GACGAAGCCGTTTTCAGTCCCTGTCGTATTCCAGCAGCAGGCGCCGCAGATCCAGCCAGCTTGCCCGGTCGGGGGCATAG
AGCAGGCCGCCGGCAAGGTC

Product: protein MazG

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MEFPPMSEATQALSRLIDIMARLRDPVAGCPWDREQDFATIAPFAIEEAYEVEDTIARQDWAALPDELGDLLLQVVYHAR
MAEEAGWFDFATVAGMIGDKMVRRHPHVFGDATLQAGQWEDEKERERQARREEGTLAGIPAALPALLRARKLSARAARVG
FDWPDARDVLDKVDEEVAELKAELDSGDRARITDELGDVLFTLATLARKLDIDPEACLRQANAKFTRRFTAMEQDLARRG
TSLPDSTLAEMEAGWQEVKRRER

Sequences:

>Translated_263_residues
MEFPPMSEATQALSRLIDIMARLRDPVAGCPWDREQDFATIAPFAIEEAYEVEDTIARQDWAALPDELGDLLLQVVYHAR
MAEEAGWFDFATVAGMIGDKMVRRHPHVFGDATLQAGQWEDEKERERQARREEGTLAGIPAALPALLRARKLSARAARVG
FDWPDARDVLDKVDEEVAELKAELDSGDRARITDELGDVLFTLATLARKLDIDPEACLRQANAKFTRRFTAMEQDLARRG
TSLPDSTLAEMEAGWQEVKRRER
>Mature_263_residues
MEFPPMSEATQALSRLIDIMARLRDPVAGCPWDREQDFATIAPFAIEEAYEVEDTIARQDWAALPDELGDLLLQVVYHAR
MAEEAGWFDFATVAGMIGDKMVRRHPHVFGDATLQAGQWEDEKERERQARREEGTLAGIPAALPALLRARKLSARAARVG
FDWPDARDVLDKVDEEVAELKAELDSGDRARITDELGDVLFTLATLARKLDIDPEACLRQANAKFTRRFTAMEQDLARRG
TSLPDSTLAEMEAGWQEVKRRER

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=257, Percent_Identity=48.2490272373541, Blast_Score=238, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 29652; Mature: 29652

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFPPMSEATQALSRLIDIMARLRDPVAGCPWDREQDFATIAPFAIEEAYEVEDTIARQD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WAALPDELGDLLLQVVYHARMAEEAGWFDFATVAGMIGDKMVRRHPHVFGDATLQAGQWE
HHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DEKERERQARREEGTLAGIPAALPALLRARKLSARAARVGFDWPDARDVLDKVDEEVAEL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KAELDSGDRARITDELGDVLFTLATLARKLDIDPEACLRQANAKFTRRFTAMEQDLARRG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TSLPDSTLAEMEAGWQEVKRRER
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFPPMSEATQALSRLIDIMARLRDPVAGCPWDREQDFATIAPFAIEEAYEVEDTIARQD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WAALPDELGDLLLQVVYHARMAEEAGWFDFATVAGMIGDKMVRRHPHVFGDATLQAGQWE
HHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DEKERERQARREEGTLAGIPAALPALLRARKLSARAARVGFDWPDARDVLDKVDEEVAEL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KAELDSGDRARITDELGDVLFTLATLARKLDIDPEACLRQANAKFTRRFTAMEQDLARRG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TSLPDSTLAEMEAGWQEVKRRER
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]