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The map label for this gene is dgoD [H]

Identifier: 162147955

GI number: 162147955

Start: 2222215

End: 2223363

Strand: Reverse

Name: dgoD [H]

Synonym: GDI_2171

Alternate gene names: 162147955

Gene position: 2223363-2222215 (Counterclockwise)

Preceding gene: 162147956

Following gene: 162147954

Centisome position: 56.37

GC content: 65.8

Gene sequence:

>1149_bases
ATGAAGATCACGAAGCTGACGACGTTCCAGGTCCCGCCGCGCTGGCTGTTCCTGAAGATCGAGACCGACGAGGGGATCAG
CGGCTGGGGTGAGCCGGTGGTCGAGGGCAGGGCCGACACCGTTGCCGCCGCCGTGGCCGAACTGGCGGACTACCTGGTCG
GCAAGGACCCGTTCCGCATCGAGGATCACTGGACCGTCCTGTATCGCGGCGGCTTCTACCGGGGCGGCGCGGTGCATATG
AGCGCCATTGCCGGTATCGATCAGGCGCTGTGGGACATCAAGGGCCGGGCCTTCGGTGTGCCGGTGCACGATCTGCTGGG
CGGGCGCTGCCGCGACCGTATCCGCGTCTATTCCTGGATCGGCGGCGACCGGCCGGCGGACACGGCCCAGGCGGTCCGCG
CCGTGGTCGATCGCGGCTTTACCGCGATCAAGATGAATGCGACCGAAGAACTGCAATATGTCGACAGCCACGCCAAGGTG
GACGACGTGATCGCCCGTGTCGCCGCGATCCGCGAGGAGGCGGGGCCCTATCTGGGCATCGGCGTGGATTTCCACGGCCG
CGTGCACAAGCCGATGGCCAAGGTCCTGGCCAGGGAACTGGAACCCTACGACCTGATGTTCATCGAGGAGCCGGTCCTGA
GCGAGCATCTGGAAGACCTGCCTGAAATCACCAAGCACACCTCGATTCCCATCGCGCTGGGCGAACGCCTGTTTTCGCGC
TGGGACTTCAAGCGGGTGTTCGAACAGGGGTGCGTGGACATCATCCAGCCCGACCCGTCGCATGCCGGCGGCATCACCGA
AACCCGCAAGATCGCGGCGATGGCGGAGGCCTATGACGTCGCGGTGGCGCTGCACTGCCCGCTGGGGCCGATCGCGCTGG
CGGCGAACCTGCAGCTCGATGCCTTGTGCTACAATGCGTTCATCCAGGAACAGAGCCTGGGCATCCACTACAACAAGACC
AACGACCTGCTGGACTATCTGGTGGATCCGGATGTCTTCGCCTATCGCGATGGGCACGTGGACATCCCGACCGGCCCCGG
CCTGGGGATCGAGATCAACGAGGACTATGTCCGCGCCCGCGCCGCCGAGGGCCATCGCTGGCGCAACCGGTCTGGCGGCA
TCGCGACGAGGTCGTTCGCGGAATGGTAG

Upstream 100 bases:

>100_bases
CGGCGGGCGAGGTCGGCGCGACGGCGCGGCTGTTTATCGCCGGCCTGGAAAAAGCCGGTCTGGAAAAATCCAGACTGGAA
AAACAGGAACGGAAACGGAC

Downstream 100 bases:

>100_bases
TGTCCCGGTCCCGACACGGGTTCCTATGCCCGCTATCCCAGCCTGGCCGGCCGTTCGGTGCTGGTCACGGGGGGCGCCAG
CGGCATCGGCGCGGCGCTGG

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITKLTTFQVPPRWLFLKIETDEGISGWGEPVVEGRADTVAAAVAELADYLVGKDPFRIEDHWTVLYRGGFYRGGAVHM
SAIAGIDQALWDIKGRAFGVPVHDLLGGRCRDRIRVYSWIGGDRPADTAQAVRAVVDRGFTAIKMNATEELQYVDSHAKV
DDVIARVAAIREEAGPYLGIGVDFHGRVHKPMAKVLARELEPYDLMFIEEPVLSEHLEDLPEITKHTSIPIALGERLFSR
WDFKRVFEQGCVDIIQPDPSHAGGITETRKIAAMAEAYDVAVALHCPLGPIALAANLQLDALCYNAFIQEQSLGIHYNKT
NDLLDYLVDPDVFAYRDGHVDIPTGPGLGIEINEDYVRARAAEGHRWRNRSGGIATRSFAEW

Sequences:

>Translated_382_residues
MKITKLTTFQVPPRWLFLKIETDEGISGWGEPVVEGRADTVAAAVAELADYLVGKDPFRIEDHWTVLYRGGFYRGGAVHM
SAIAGIDQALWDIKGRAFGVPVHDLLGGRCRDRIRVYSWIGGDRPADTAQAVRAVVDRGFTAIKMNATEELQYVDSHAKV
DDVIARVAAIREEAGPYLGIGVDFHGRVHKPMAKVLARELEPYDLMFIEEPVLSEHLEDLPEITKHTSIPIALGERLFSR
WDFKRVFEQGCVDIIQPDPSHAGGITETRKIAAMAEAYDVAVALHCPLGPIALAANLQLDALCYNAFIQEQSLGIHYNKT
NDLLDYLVDPDVFAYRDGHVDIPTGPGLGIEINEDYVRARAAEGHRWRNRSGGIATRSFAEW
>Mature_382_residues
MKITKLTTFQVPPRWLFLKIETDEGISGWGEPVVEGRADTVAAAVAELADYLVGKDPFRIEDHWTVLYRGGFYRGGAVHM
SAIAGIDQALWDIKGRAFGVPVHDLLGGRCRDRIRVYSWIGGDRPADTAQAVRAVVDRGFTAIKMNATEELQYVDSHAKV
DDVIARVAAIREEAGPYLGIGVDFHGRVHKPMAKVLARELEPYDLMFIEEPVLSEHLEDLPEITKHTSIPIALGERLFSR
WDFKRVFEQGCVDIIQPDPSHAGGITETRKIAAMAEAYDVAVALHCPLGPIALAANLQLDALCYNAFIQEQSLGIHYNKT
NDLLDYLVDPDVFAYRDGHVDIPTGPGLGIEINEDYVRARAAEGHRWRNRSGGIATRSFAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=57.8534031413613, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1787864, Length=385, Percent_Identity=32.2077922077922, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI226510960, Length=279, Percent_Identity=29.7491039426523, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42428; Mature: 42428

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKLTTFQVPPRWLFLKIETDEGISGWGEPVVEGRADTVAAAVAELADYLVGKDPFRI
CCEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
EDHWTVLYRGGFYRGGAVHMSAIAGIDQALWDIKGRAFGVPVHDLLGGRCRDRIRVYSWI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCHHHHHEEEEEEC
GGDRPADTAQAVRAVVDRGFTAIKMNATEELQYVDSHAKVDDVIARVAAIREEAGPYLGI
CCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GVDFHGRVHKPMAKVLARELEPYDLMFIEEPVLSEHLEDLPEITKHTSIPIALGERLFSR
EECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WDFKRVFEQGCVDIIQPDPSHAGGITETRKIAAMAEAYDVAVALHCPLGPIALAANLQLD
HHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCHH
ALCYNAFIQEQSLGIHYNKTNDLLDYLVDPDVFAYRDGHVDIPTGPGLGIEINEDYVRAR
HHHHHHHHHHCCCCEEECCHHHHHHHHCCCCEEEEECCEEECCCCCCCEEEECHHHHHHH
AAEGHRWRNRSGGIATRSFAEW
HCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITKLTTFQVPPRWLFLKIETDEGISGWGEPVVEGRADTVAAAVAELADYLVGKDPFRI
CCEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
EDHWTVLYRGGFYRGGAVHMSAIAGIDQALWDIKGRAFGVPVHDLLGGRCRDRIRVYSWI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCHHHHHEEEEEEC
GGDRPADTAQAVRAVVDRGFTAIKMNATEELQYVDSHAKVDDVIARVAAIREEAGPYLGI
CCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GVDFHGRVHKPMAKVLARELEPYDLMFIEEPVLSEHLEDLPEITKHTSIPIALGERLFSR
EECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WDFKRVFEQGCVDIIQPDPSHAGGITETRKIAAMAEAYDVAVALHCPLGPIALAANLQLD
HHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCHH
ALCYNAFIQEQSLGIHYNKTNDLLDYLVDPDVFAYRDGHVDIPTGPGLGIEINEDYVRAR
HHHHHHHHHHCCCCEEECCHHHHHHHHCCCCEEEEECCEEECCCCCCCEEEECHHHHHHH
AAEGHRWRNRSGGIATRSFAEW
HCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA