BacMap

Definition	Gluconacetobacter diazotrophicus PAl 5 chromosome, complete genome.
Accession	NC_010125
Length	3,944,163

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The map label for this gene is linX [H]

Identifier: 162147954

GI number: 162147954

Start: 2221435

End: 2222157

Strand: Reverse

Name: linX [H]

Synonym: GDI_2170

Alternate gene names: 162147954

Gene position: 2222157-2221435 (Counterclockwise)

Preceding gene: 162147955

Following gene: 162147953

Centisome position: 56.34

GC content: 71.65

Gene sequence:

>723_bases
GTGCTGGTCACGGGGGGCGCCAGCGGCATCGGCGCGGCGCTGGTCCGCGCCTTCGCCGACCAGGGCGCGCGGGTGGCCTT
CGTGGACCTGGACCGGGTAGCGGCGGACGCTCTGGCGGGCGAGGTCGCCCAGGCGGGCCGGCAGGTGCTGTTCATCCCGT
GCGACGTGACCGATGTCGCGGCGTTGCGCGAGGCGGTCGCCACGGCCTTCGGTGCGCACGGGCCGGTGGACATCCTGCTG
AACAATGCCGGCAACGATGCCCGCCACACGCTGGACGAGGTCGATGTCGCGATGTGGGAGCGCCTGTCCGCGATCAATAT
CCGCGCCCAGTTCTTCGCGGCCCAGGCCGTGGCGCCCGGGATGCGGGCGGCGGGGCGCGGGTCGATCGTCTGCCTGGGGT
CGACGGGGTGGATGCAGAAGAACGCCGGCTATCCCATCTATGTCACCGCCAAGGCGGCGGTGCTGGGGCTGGTGAACGCC
CTGGCGCGCGAACTGGGCCAGGACCATATCCGGGTCAACGCGCTGGTTCCCGGCTGGGTGATGACCGAACGCCAGCGCCG
CCTGTGGCTGACACCCGAGGGCGAGCGGCAGATCGACCGCAGCCAGTGCCTGCCGGGCCGGGTGGAACCCGGCGACATCG
CGCGCATGGCCCTGTTCCTGGCTGCCGATGACAGCCGGATGTGCAGCGGCCAGCAATTCATCGTCGATGGCGGCTGGGTC
TAG

Upstream 100 bases:

>100_bases
CCGGTCTGGCGGCATCGCGACGAGGTCGTTCGCGGAATGGTAGTGTCCCGGTCCCGACACGGGTTCCTATGCCCGCTATC
CCAGCCTGGCCGGCCGTTCG

Downstream 100 bases:

>100_bases
GCGCGGTCGGCGGTTTACGCCCGAAACATGCAAAAAGACGAACCGTATCGTCGCCGGTCCGTTATAATCGCTCAAAAACG
GAGCAGAACATGGAGGCGTA

Product: oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLVTGGASGIGAALVRAFADQGARVAFVDLDRVAADALAGEVAQAGRQVLFIPCDVTDVAALREAVATAFGAHGPVDILL
NNAGNDARHTLDEVDVAMWERLSAINIRAQFFAAQAVAPGMRAAGRGSIVCLGSTGWMQKNAGYPIYVTAKAAVLGLVNA
LARELGQDHIRVNALVPGWVMTERQRRLWLTPEGERQIDRSQCLPGRVEPGDIARMALFLAADDSRMCSGQQFIVDGGWV

Sequences:

>Translated_240_residues
MLVTGGASGIGAALVRAFADQGARVAFVDLDRVAADALAGEVAQAGRQVLFIPCDVTDVAALREAVATAFGAHGPVDILL
NNAGNDARHTLDEVDVAMWERLSAINIRAQFFAAQAVAPGMRAAGRGSIVCLGSTGWMQKNAGYPIYVTAKAAVLGLVNA
LARELGQDHIRVNALVPGWVMTERQRRLWLTPEGERQIDRSQCLPGRVEPGDIARMALFLAADDSRMCSGQQFIVDGGWV
>Mature_240_residues
MLVTGGASGIGAALVRAFADQGARVAFVDLDRVAADALAGEVAQAGRQVLFIPCDVTDVAALREAVATAFGAHGPVDILL
NNAGNDARHTLDEVDVAMWERLSAINIRAQFFAAQAVAPGMRAAGRGSIVCLGSTGWMQKNAGYPIYVTAKAAVLGLVNA
LARELGQDHIRVNALVPGWVMTERQRRLWLTPEGERQIDRSQCLPGRVEPGDIARMALFLAADDSRMCSGQQFIVDGGWV

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=238, Percent_Identity=32.7731092436975, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI126723750, Length=241, Percent_Identity=31.1203319502075, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI15277342, Length=253, Percent_Identity=32.0158102766798, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI7705925, Length=240, Percent_Identity=32.5, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI126723191, Length=196, Percent_Identity=31.6326530612245, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=27.5720164609054, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI31542939, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI19923817, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI66933014, Length=228, Percent_Identity=28.9473684210526, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI40254992, Length=237, Percent_Identity=27.8481012658228, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4504505, Length=251, Percent_Identity=27.4900398406374, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=29.4372294372294, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI33667109, Length=183, Percent_Identity=29.5081967213115, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=28.6274509803922, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI109715829, Length=232, Percent_Identity=29.3103448275862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI302344755, Length=108, Percent_Identity=37.962962962963, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4503817, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI20149619, Length=192, Percent_Identity=26.5625, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787905, Length=239, Percent_Identity=34.3096234309623, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082100, Length=244, Percent_Identity=33.1967213114754, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787335, Length=239, Percent_Identity=30.1255230125523, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1790717, Length=241, Percent_Identity=29.045643153527, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789208, Length=242, Percent_Identity=28.9256198347107, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082160, Length=245, Percent_Identity=28.9795918367347, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1789378, Length=240, Percent_Identity=28.3333333333333, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786812, Length=244, Percent_Identity=29.0983606557377, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI2367365, Length=244, Percent_Identity=31.5573770491803, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788459, Length=243, Percent_Identity=25.5144032921811, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787526, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787820, Length=184, Percent_Identity=29.8913043478261, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789057, Length=259, Percent_Identity=27.7992277992278, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=240, Percent_Identity=30.4166666666667, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17555706, Length=245, Percent_Identity=33.8775510204082, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17561402, Length=242, Percent_Identity=29.7520661157025, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=32.2448979591837, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=257, Percent_Identity=29.1828793774319, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=252, Percent_Identity=27.3809523809524, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=256, Percent_Identity=30.46875, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560676, Length=239, Percent_Identity=25.1046025104602, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=186, Percent_Identity=28.494623655914, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=257, Percent_Identity=31.5175097276265, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI72000259, Length=253, Percent_Identity=28.4584980237154, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=259, Percent_Identity=29.3436293436293, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=257, Percent_Identity=28.0155642023346, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=260, Percent_Identity=28.0769230769231, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=25.7028112449799, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=28.4615384615385, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=252, Percent_Identity=28.1746031746032, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=25.0996015936255, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=175, Percent_Identity=28, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=255, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=253, Percent_Identity=25.6916996047431, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=256, Percent_Identity=26.5625, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=259, Percent_Identity=27.4131274131274, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=28.7313432835821, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=203, Percent_Identity=25.1231527093596, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=256, Percent_Identity=25.390625, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=184, Percent_Identity=25, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=184, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=258, Percent_Identity=28.6821705426357, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=196, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=219, Percent_Identity=27.8538812785388, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357041, Length=244, Percent_Identity=28.6885245901639, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI23397609, Length=247, Percent_Identity=30.3643724696356, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571526, Length=243, Percent_Identity=27.9835390946502, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355319, Length=241, Percent_Identity=25.3112033195021, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639444, Length=243, Percent_Identity=30.0411522633745, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644339, Length=247, Percent_Identity=27.9352226720648, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17737361, Length=258, Percent_Identity=29.8449612403101, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18859837, Length=185, Percent_Identity=29.7297297297297, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364892, Length=193, Percent_Identity=30.5699481865285, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24643142, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358495, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24640492, Length=186, Percent_Identity=26.8817204301075, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24644337, Length=228, Percent_Identity=26.7543859649123, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 25451; Mature: 25451

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTGGASGIGAALVRAFADQGARVAFVDLDRVAADALAGEVAQAGRQVLFIPCDVTDVA
CEEECCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
ALREAVATAFGAHGPVDILLNNAGNDARHTLDEVDVAMWERLSAINIRAQFFAAQAVAPG
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MRAAGRGSIVCLGSTGWMQKNAGYPIYVTAKAAVLGLVNALARELGQDHIRVNALVPGWV
CCCCCCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCHH
MTERQRRLWLTPEGERQIDRSQCLPGRVEPGDIARMALFLAADDSRMCSGQQFIVDGGWV
EECCCCEEEECCCCCHHCCHHHCCCCCCCCCHHHHEEEEEECCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MLVTGGASGIGAALVRAFADQGARVAFVDLDRVAADALAGEVAQAGRQVLFIPCDVTDVA
CEEECCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
ALREAVATAFGAHGPVDILLNNAGNDARHTLDEVDVAMWERLSAINIRAQFFAAQAVAPG
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MRAAGRGSIVCLGSTGWMQKNAGYPIYVTAKAAVLGLVNALARELGQDHIRVNALVPGWV
CCCCCCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCHH
MTERQRRLWLTPEGERQIDRSQCLPGRVEPGDIARMALFLAADDSRMCSGQQFIVDGGWV
EECCCCEEEECCCCCHHCCHHHCCCCCCCCCHHHHEEEEEECCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]