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The map label for this gene is lpxD [H]

Identifier: 162147927

GI number: 162147927

Start: 2194680

End: 2195750

Strand: Reverse

Name: lpxD [H]

Synonym: GDI_2143

Alternate gene names: 162147927

Gene position: 2195750-2194680 (Counterclockwise)

Preceding gene: 162147928

Following gene: 162147926

Centisome position: 55.67

GC content: 68.25

Gene sequence:

>1071_bases
ATGGAAAGGCAGGACGCGGCCCTGCCGGGGGACCGGCGGTTCTTCGAGCGGAACGGCCCGTTCTCGCTCCGGGCACTGGC
GGAATGCGCCGGGGCCAGGGTCGAAGGCGGGGATGAGGCAGACGACGCCCCCCGCTATGTCGGGATCGCGCCGCTGCAGT
CGGCCGGCCCGCAAGAGGTCAGCTTCCTCGACAACCGGCGCTATGCGCCGCTGCTGGAACAGACGCGGGCGGGCGTGGTG
ATCCTTGCCCCCGCCTTCGTCGACCGTCTGCCCAAGGGTACGGTGGGGCTGGTGTGTTCGACGCCCTATCTGGCGTGGGC
GCGCGTGGCTGGCCTGTTCCACCCGGTCCCGGCCGCGATCCCGGGTATCCATCCCACCGCCATCGTCGGCGCCGGCGCCG
ACATCGATCCGTCGGCGCAGATCGGTCCATTCGTGACCATCGGCGCGGGCGCGCAGGTGGGGGCGGGCAGCCGGATCGAC
GCCTATGCGCTGATCGGAGACGGGGTGCGGATCGGCGCGCATTGCCGGATCGGCAGCCACGCCAGCGTGTCGCATGCGCT
GCTGGGCGACCGGGTCACGCTGCTGTCCGGCGCGCGGATCGGCCAGGAAGGGTTCGGCTTCGCGGTAGGGCCCGACGGGT
TCGAGACCGTGCCGCAGTTGGGCCGCGTGGTGCTGGAAGACGGGGTGGAGGTCGGAGCGAATTCGACCATCGATCGCGGT
TCGTCCCAGGACACCGTCATCGGCGCGGGCTCACGCCTCGACAACCTGGTGCAGATTGGCCATAATGCCCGCCTCGGGCG
GTGTTGTATCGTCGTTTCCCAGGCCGGTATTTCAGGTTCGACGGAACTGGGCGATTTTGTCACCATCGCCGCCCAGGCCG
GTCTGATCGGTCATATTCGTGTTGGCACCAAGGCCCGTATCGGTGCGCAATGCGGCGTGATGTCGGATGTGGAAGCAGGG
GCCGATGTTATCGGTAGCCCTGCGATGCCGTTTCGGGAATTTTTCCGGAACGTGGCCGTGTTGCGGCGCCTGGCCAAAAA
GGCGACGCAAAATGGAGGGGGCGAGACCTGA

Upstream 100 bases:

>100_bases
GCACGATGCCGACGACGGCCGATGCCGGCCGGCAGGCGGCGCCTGCCCCGACACCCGCGCCGGTCGCGGCGACGGCCCGG
CACTGACGGGCGCCAGCGCA

Downstream 100 bases:

>100_bases
GGCTGCTGGCCTCCGAACAAGGCCCCCGGAATTCAACCGGGACAGGCGGGCGACGGTCGCAAGGATGACGTGTCCCGCTA
TCCCGAGCAAGATGGTCGGA

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MERQDAALPGDRRFFERNGPFSLRALAECAGARVEGGDEADDAPRYVGIAPLQSAGPQEVSFLDNRRYAPLLEQTRAGVV
ILAPAFVDRLPKGTVGLVCSTPYLAWARVAGLFHPVPAAIPGIHPTAIVGAGADIDPSAQIGPFVTIGAGAQVGAGSRID
AYALIGDGVRIGAHCRIGSHASVSHALLGDRVTLLSGARIGQEGFGFAVGPDGFETVPQLGRVVLEDGVEVGANSTIDRG
SSQDTVIGAGSRLDNLVQIGHNARLGRCCIVVSQAGISGSTELGDFVTIAAQAGLIGHIRVGTKARIGAQCGVMSDVEAG
ADVIGSPAMPFREFFRNVAVLRRLAKKATQNGGGET

Sequences:

>Translated_356_residues
MERQDAALPGDRRFFERNGPFSLRALAECAGARVEGGDEADDAPRYVGIAPLQSAGPQEVSFLDNRRYAPLLEQTRAGVV
ILAPAFVDRLPKGTVGLVCSTPYLAWARVAGLFHPVPAAIPGIHPTAIVGAGADIDPSAQIGPFVTIGAGAQVGAGSRID
AYALIGDGVRIGAHCRIGSHASVSHALLGDRVTLLSGARIGQEGFGFAVGPDGFETVPQLGRVVLEDGVEVGANSTIDRG
SSQDTVIGAGSRLDNLVQIGHNARLGRCCIVVSQAGISGSTELGDFVTIAAQAGLIGHIRVGTKARIGAQCGVMSDVEAG
ADVIGSPAMPFREFFRNVAVLRRLAKKATQNGGGET
>Mature_356_residues
MERQDAALPGDRRFFERNGPFSLRALAECAGARVEGGDEADDAPRYVGIAPLQSAGPQEVSFLDNRRYAPLLEQTRAGVV
ILAPAFVDRLPKGTVGLVCSTPYLAWARVAGLFHPVPAAIPGIHPTAIVGAGADIDPSAQIGPFVTIGAGAQVGAGSRID
AYALIGDGVRIGAHCRIGSHASVSHALLGDRVTLLSGARIGQEGFGFAVGPDGFETVPQLGRVVLEDGVEVGANSTIDRG
SSQDTVIGAGSRLDNLVQIGHNARLGRCCIVVSQAGISGSTELGDFVTIAAQAGLIGHIRVGTKARIGAQCGVMSDVEAG
ADVIGSPAMPFREFFRNVAVLRRLAKKATQNGGGET

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=316, Percent_Identity=30.379746835443, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786378, Length=212, Percent_Identity=25.9433962264151, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36660; Mature: 36660

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQDAALPGDRRFFERNGPFSLRALAECAGARVEGGDEADDAPRYVGIAPLQSAGPQEV
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHH
SFLDNRRYAPLLEQTRAGVVILAPAFVDRLPKGTVGLVCSTPYLAWARVAGLFHPVPAAI
HHHCCCCCCHHHHHCCCCEEEECHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCHHHC
PGIHPTAIVGAGADIDPSAQIGPFVTIGAGAQVGAGSRIDAYALIGDGVRIGAHCRIGSH
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEEEECCCC
ASVSHALLGDRVTLLSGARIGQEGFGFAVGPDGFETVPQLGRVVLEDGVEVGANSTIDRG
CCHHHHHHCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
SSQDTVIGAGSRLDNLVQIGHNARLGRCCIVVSQAGISGSTELGDFVTIAAQAGLIGHIR
CCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHEEEEECCCCEEEEE
VGTKARIGAQCGVMSDVEAGADVIGSPAMPFREFFRNVAVLRRLAKKATQNGGGET
ECCCCCCCCCCCCCCCHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MERQDAALPGDRRFFERNGPFSLRALAECAGARVEGGDEADDAPRYVGIAPLQSAGPQEV
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHH
SFLDNRRYAPLLEQTRAGVVILAPAFVDRLPKGTVGLVCSTPYLAWARVAGLFHPVPAAI
HHHCCCCCCHHHHHCCCCEEEECHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCHHHC
PGIHPTAIVGAGADIDPSAQIGPFVTIGAGAQVGAGSRIDAYALIGDGVRIGAHCRIGSH
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEEEECCCC
ASVSHALLGDRVTLLSGARIGQEGFGFAVGPDGFETVPQLGRVVLEDGVEVGANSTIDRG
CCHHHHHHCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
SSQDTVIGAGSRLDNLVQIGHNARLGRCCIVVSQAGISGSTELGDFVTIAAQAGLIGHIR
CCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHEEEEECCCCEEEEE
VGTKARIGAQCGVMSDVEAGADVIGSPAMPFREFFRNVAVLRRLAKKATQNGGGET
ECCCCCCCCCCCCCCCHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA