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The map label for this gene is gshB [H]

Identifier: 162147887

GI number: 162147887

Start: 2151815

End: 2152819

Strand: Reverse

Name: gshB [H]

Synonym: GDI_2103

Alternate gene names: 162147887

Gene position: 2152819-2151815 (Counterclockwise)

Preceding gene: 162147888

Following gene: 162147886

Centisome position: 54.58

GC content: 66.17

Gene sequence:

>1005_bases
ATGTCCCGCCCGTTGAAGGTCGCCGTACAGATGGATCCCCTCGCCGGGATCGACATCAATGGCGACTCCACCTTTGCCCT
GATGCTGGAGGCGCAGGCCCGGGGATACGAACTGTTCGTCTACCATGTGCAGGGCCTGAGCCTGCGCGAGGGACGCACCG
ACGGGCATGCTGGCGGCACGCGAACCGAGCGGCTGACCGCACGGGCGCGCCCCGCACGCGTCCAGCGCGTGGCGGGCGAT
CACGCGGCGCTGGGCGAGGAACGGGTGCTGGACCTGTCGGGGATGGACGTGATCCTGATGCGTCAGGACCCGCCGTTCGA
CATGGCCTATATTACCGCCACGCATATGCTGGAACATGTCCACGGTACCGGCCCGGGCCGGGCGCTGGTGGTCAACGACC
CGGAATCGGTGCGCAACGCGCCGGAAAAGCTGCTGGTCACGCATTATCCCGACCTGATGCCGCCCACGCTGGTGACGTGG
GACGTCCAAGCCATCCGCGATTTCCGCGCCCAGTGGCGGGACATCATCGTCAAGCCGCTGTTCGGCAATGGCGGCGCGGG
CGTCTTCCGCATCCGTGAGGATGACGAAAACCTGAATTCCCTGCTGGAGATGCATTTCGCCCGCTCGCGCGAGCCCCTGA
TGATCCAGCGGTACGAGGCCGCGGTCCGGCGCGGCGACAAGCGTATCATCCTGGCGGACGGCCAGCCGATCGGTGCCATC
AACCGCGTTCCCGCGCAGGGCGAGGCCCGGTCGAACATGCATGTGGGCGGCAAGGCGGCGAAGGTGGAACTGACCCCGCG
CGACCGCGAGATCTGCGAGGCGATCGGACCGATGCTGCGTGAAAAGGGATTGATCTTCGTCGGGATCGACGTGATCGGCG
ACTGGCTGACCGAGATCAATGTGACGTCGCCGACCGGCCTGCAGGAAATAGACCGGTTCGATGATATCAACTCGGCGGGC
CTGATCTGGGACTGCATCCTGCGCCGGCTGGATGGCGGGGCTTGA

Upstream 100 bases:

>100_bases
TGGTATGAGATTTTTCGTTGCAAAGAAATTCGACTAAGAAGCAGGGGCGGATGGCGCCGTTCGTCGTCCGCGGTCCGAAT
CGTCGCCGGAGGCTGCGTCC

Downstream 100 bases:

>100_bases
TCGGGTGGGCGAGGGGATGCGGCCAAGGTGGCACGAACAAATGGGAAGGGCAGGCATTTTGCGGCGCGACAGCGGACTTT
GGTGCGACGGCCACTTGAAG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSRPLKVAVQMDPLAGIDINGDSTFALMLEAQARGYELFVYHVQGLSLREGRTDGHAGGTRTERLTARARPARVQRVAGD
HAALGEERVLDLSGMDVILMRQDPPFDMAYITATHMLEHVHGTGPGRALVVNDPESVRNAPEKLLVTHYPDLMPPTLVTW
DVQAIRDFRAQWRDIIVKPLFGNGGAGVFRIREDDENLNSLLEMHFARSREPLMIQRYEAAVRRGDKRIILADGQPIGAI
NRVPAQGEARSNMHVGGKAAKVELTPRDREICEAIGPMLREKGLIFVGIDVIGDWLTEINVTSPTGLQEIDRFDDINSAG
LIWDCILRRLDGGA

Sequences:

>Translated_334_residues
MSRPLKVAVQMDPLAGIDINGDSTFALMLEAQARGYELFVYHVQGLSLREGRTDGHAGGTRTERLTARARPARVQRVAGD
HAALGEERVLDLSGMDVILMRQDPPFDMAYITATHMLEHVHGTGPGRALVVNDPESVRNAPEKLLVTHYPDLMPPTLVTW
DVQAIRDFRAQWRDIIVKPLFGNGGAGVFRIREDDENLNSLLEMHFARSREPLMIQRYEAAVRRGDKRIILADGQPIGAI
NRVPAQGEARSNMHVGGKAAKVELTPRDREICEAIGPMLREKGLIFVGIDVIGDWLTEINVTSPTGLQEIDRFDDINSAG
LIWDCILRRLDGGA
>Mature_333_residues
SRPLKVAVQMDPLAGIDINGDSTFALMLEAQARGYELFVYHVQGLSLREGRTDGHAGGTRTERLTARARPARVQRVAGDH
AALGEERVLDLSGMDVILMRQDPPFDMAYITATHMLEHVHGTGPGRALVVNDPESVRNAPEKLLVTHYPDLMPPTLVTWD
VQAIRDFRAQWRDIIVKPLFGNGGAGVFRIREDDENLNSLLEMHFARSREPLMIQRYEAAVRRGDKRIILADGQPIGAIN
RVPAQGEARSNMHVGGKAAKVELTPRDREICEAIGPMLREKGLIFVGIDVIGDWLTEINVTSPTGLQEIDRFDDINSAGL
IWDCILRRLDGGA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=327, Percent_Identity=41.5902140672783, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 36992; Mature: 36861

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPLKVAVQMDPLAGIDINGDSTFALMLEAQARGYELFVYHVQGLSLREGRTDGHAGGT
CCCCEEEEEEECCCCCEEECCCCEEEEEEEECCCCEEEEEEEECCCEEECCCCCCCCCCC
RTERLTARARPARVQRVAGDHAALGEERVLDLSGMDVILMRQDPPFDMAYITATHMLEHV
CHHHHHHCCCHHHHHHHCCCHHHCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
HGTGPGRALVVNDPESVRNAPEKLLVTHYPDLMPPTLVTWDVQAIRDFRAQWRDIIVKPL
CCCCCCCEEEECCCHHHHCCCHHEEEECCCCCCCCCEEEECHHHHHHHHHHHHHEEEEEE
FGNGGAGVFRIREDDENLNSLLEMHFARSREPLMIQRYEAAVRRGDKRIILADGQPIGAI
ECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCC
NRVPAQGEARSNMHVGGKAAKVELTPRDREICEAIGPMLREKGLIFVGIDVIGDWLTEIN
CCCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHEE
VTSPTGLQEIDRFDDINSAGLIWDCILRRLDGGA
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRPLKVAVQMDPLAGIDINGDSTFALMLEAQARGYELFVYHVQGLSLREGRTDGHAGGT
CCCEEEEEEECCCCCEEECCCCEEEEEEEECCCCEEEEEEEECCCEEECCCCCCCCCCC
RTERLTARARPARVQRVAGDHAALGEERVLDLSGMDVILMRQDPPFDMAYITATHMLEHV
CHHHHHHCCCHHHHHHHCCCHHHCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
HGTGPGRALVVNDPESVRNAPEKLLVTHYPDLMPPTLVTWDVQAIRDFRAQWRDIIVKPL
CCCCCCCEEEECCCHHHHCCCHHEEEECCCCCCCCCEEEECHHHHHHHHHHHHHEEEEEE
FGNGGAGVFRIREDDENLNSLLEMHFARSREPLMIQRYEAAVRRGDKRIILADGQPIGAI
ECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCC
NRVPAQGEARSNMHVGGKAAKVELTPRDREICEAIGPMLREKGLIFVGIDVIGDWLTEIN
CCCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHEE
VTSPTGLQEIDRFDDINSAGLIWDCILRRLDGGA
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]