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The map label for this gene is grepB [H]

Identifier: 162147888

GI number: 162147888

Start: 2152912

End: 2153511

Strand: Reverse

Name: grepB [H]

Synonym: GDI_2104

Alternate gene names: 162147888

Gene position: 2153511-2152912 (Counterclockwise)

Preceding gene: 162147889

Following gene: 162147887

Centisome position: 54.6

GC content: 66.83

Gene sequence:

>600_bases
GTGGCGCTCGCGCTCACCAGCCCACGTTTCCGGACCAACATCGAGGCCTGTTTTGCGATGAGTGACGACGACAATGATGA
CGACGATGCGGCACCCCGGCGGCTGGAGGGGGTGTCGCGTTATGTCACGCCCGCGGGCATGGCGGCGATGCGGGGGGAAC
TGAACGCGCTGCTGCGCGATGAACGGCCCCGGATCGTCGATATCGTGTCCTGGGCGGCGGGCAATGGCGATCGGTCGGAA
AATGGCGACTACCTGTACGGCAAGAAGCGCCTGCGCGAGATCGACCGGCGCGTGCGCTTCCTGACCCGTCGGATCGAAAA
GGCGATCGTGGTCGATCCCGCCGCCCAGACCCGGCGTGACCGCGTGTTCTTCGGTGCGACCGTCACCTATGTGACCGAGG
ACGATACCGAACGGACGGTCGTCATCGTGGGCGTGGATGAATCCGACCTGGCGGCGGGCCAGGTCAGCCTGGCATCGCCC
GTGGCCCGTGCGCTGCTGGGCGGCCGGGTGGGGGACGAGGTCCGTCTGCTGACTCCGGCGGGGGCCGAGATGATCGAAAT
CCTGCGGATTGCCTATCCCGCCCCGGCTGAAATGGTATGA

Upstream 100 bases:

>100_bases
CAAAGCTGGAGGGACATATCCGCATCGCCCTGATGGCCTGTTAGGATGTTGGTCCGGAAACGCGCGCTGGTGGCGATCCG
ACGCGTGTTTCCTGCGCTTC

Downstream 100 bases:

>100_bases
GATTTTTCGTTGCAAAGAAATTCGACTAAGAAGCAGGGGCGGATGGCGCCGTTCGTCGTCCGCGGTCCGAATCGTCGCCG
GAGGCTGCGTCCATGTCCCG

Product: transcription elongation factor greB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MALALTSPRFRTNIEACFAMSDDDNDDDDAAPRRLEGVSRYVTPAGMAAMRGELNALLRDERPRIVDIVSWAAGNGDRSE
NGDYLYGKKRLREIDRRVRFLTRRIEKAIVVDPAAQTRRDRVFFGATVTYVTEDDTERTVVIVGVDESDLAAGQVSLASP
VARALLGGRVGDEVRLLTPAGAEMIEILRIAYPAPAEMV

Sequences:

>Translated_199_residues
MALALTSPRFRTNIEACFAMSDDDNDDDDAAPRRLEGVSRYVTPAGMAAMRGELNALLRDERPRIVDIVSWAAGNGDRSE
NGDYLYGKKRLREIDRRVRFLTRRIEKAIVVDPAAQTRRDRVFFGATVTYVTEDDTERTVVIVGVDESDLAAGQVSLASP
VARALLGGRVGDEVRLLTPAGAEMIEILRIAYPAPAEMV
>Mature_198_residues
ALALTSPRFRTNIEACFAMSDDDNDDDDAAPRRLEGVSRYVTPAGMAAMRGELNALLRDERPRIVDIVSWAAGNGDRSEN
GDYLYGKKRLREIDRRVRFLTRRIEKAIVVDPAAQTRRDRVFFGATVTYVTEDDTERTVVIVGVDESDLAAGQVSLASPV
ARALLGGRVGDEVRLLTPAGAEMIEILRIAYPAPAEMV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=46.7532467532467, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=33.5526315789474, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 21912; Mature: 21781

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALALTSPRFRTNIEACFAMSDDDNDDDDAAPRRLEGVSRYVTPAGMAAMRGELNALLRD
CEEECCCCCHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
ERPRIVDIVSWAAGNGDRSENGDYLYGKKRLREIDRRVRFLTRRIEKAIVVDPAAQTRRD
CCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHCCC
RVFFGATVTYVTEDDTERTVVIVGVDESDLAAGQVSLASPVARALLGGRVGDEVRLLTPA
EEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCC
GAEMIEILRIAYPAPAEMV
CHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALALTSPRFRTNIEACFAMSDDDNDDDDAAPRRLEGVSRYVTPAGMAAMRGELNALLRD
EEECCCCCHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
ERPRIVDIVSWAAGNGDRSENGDYLYGKKRLREIDRRVRFLTRRIEKAIVVDPAAQTRRD
CCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHCCC
RVFFGATVTYVTEDDTERTVVIVGVDESDLAAGQVSLASPVARALLGGRVGDEVRLLTPA
EEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCC
GAEMIEILRIAYPAPAEMV
CHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]