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The map label for this gene is pmbA [H]

Identifier: 162147874

GI number: 162147874

Start: 2140417

End: 2141757

Strand: Direct

Name: pmbA [H]

Synonym: GDI_2090

Alternate gene names: 162147874

Gene position: 2140417-2141757 (Clockwise)

Preceding gene: 162147873

Following gene: 162147875

Centisome position: 54.27

GC content: 71.14

Gene sequence:

>1341_bases
ATGAGCACCGATCCGCTCGACCTCCTCGGCACCCTGATCGGCAAGGCCCGGGCGGCCGGTGCCGACGCGGCGGATGCCGT
CTACATGGCGCGGACCACGCACGGCGTGCAGGTCCGCAACGGCCGGACCGAGGACCTGGAACGGTCCGAGACCAGCGACC
TGGGGCTGCGGGTCTTCGTCGGCCGCCGCTCGGCCATCGTCTCGGCCACCAGCCTGGACCCGGAACGCTTCGACCCGCTG
GTCGCCCAGGCGCTGGCCATGGCCCGCGTGGTGCCCGAGGACCCGCATTCCGGCCTGTCCCCCGACGCGGTGCAGGGCTT
CGTGGACGCGGCGGGGATGGACCTGCTGGACCCGGCGCAGCCCGACACCGCCGCCCTGCTGGACCGCGCGCGCGCGGCCG
AGGACGCGGCGGTATCGGTCGCGGGCGTCACCAACAGCAATGGCGGATCGGCCTCGTCGGGCCTGTCCGACATCATCCTG
ATGACCTCGGCCGGGTTTTCCGGCCGCTACGCCCGCAGCAGCCATTCGGTGTCGGCCAGCGTCCTGGCCGGACAGGGCAC
CGGCATGCAGCGGGATTACGACTATCACGCGACGGTGCATCTGTCCGATCTGGACGATCCCGCCAAGATCGGGCGCAGCG
CCGGCGAGAAGGCGGTGGCCCGGCTGAACCCGACCCGGCCGCGCACCGGCAGCATGGCGGTGGTGTTCGACCCGCGCGTA
TCCTCCAGCCTGCTGGGCCATCTGGCGGGGGCCGTGAACGGCAGCGCCATCGCCCGGGGCACCTCGTTCCTGAAGGACCG
GATGGGGCAGCGGATCATGCCGGCCGGCATCCACATCATCGACGACCCGACGCAGTCGCGCGGCCTGTCAGCCCACCCGT
TCGACGGCGAGGGCATGCGCGCGGGCGAACTGGTCATCGTGCAGGACGGGGTACTGATGAACTGGGCGCTGGATTCCCGC
AGCGCGCGGCAGGTCGGGCTGCCCGGCAACGGCCGGGCCAGCCGGGGGACCACGGCCCCTCCGGGACCGTCGCTGGGCAG
CCTGTATGCCCGGCCCGGCACCGTGACACCGGCCGCGCTGATGGCGGACATTGCCGAGGGCATCTACGTGACCGAACTGA
TGGGATCGGCCATCAACGGCCTGACCGGCGATTACAGCCGGGGCGCCTCCGGCTTCATGATCCGTGGCGGCACGCTGGCC
GAACCGGTTGCCGAATTGACCATCGCCGGCAACCTGAACGACATGTTTGCCCGCATGGTCCTGGCGGACGATCTGGTATT
CCGCCGTGGCATCAACGCGCCCACGATCCGGATCGACGATCTGATGATCGCCGGTTCCTGA

Upstream 100 bases:

>100_bases
GGGTGGCCACGCGGCTGTTCCGGGGCAAGCCCACCCTGGCCTCGCTGGGGCCGGTGCGCAACATTCCCGGCATCGCCGCC
ATTGCCGAGGCCCTGGCGGC

Downstream 100 bases:

>100_bases
CCCAGCGATACGGGATGGACAGATCCATGAGCACACCGACCACGCCCGCCCGTCCGCCCCGCCCCCGTCGCGCGGCGATC
CTGCTGCTGGGCACCGCGTT

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSTDPLDLLGTLIGKARAAGADAADAVYMARTTHGVQVRNGRTEDLERSETSDLGLRVFVGRRSAIVSATSLDPERFDPL
VAQALAMARVVPEDPHSGLSPDAVQGFVDAAGMDLLDPAQPDTAALLDRARAAEDAAVSVAGVTNSNGGSASSGLSDIIL
MTSAGFSGRYARSSHSVSASVLAGQGTGMQRDYDYHATVHLSDLDDPAKIGRSAGEKAVARLNPTRPRTGSMAVVFDPRV
SSSLLGHLAGAVNGSAIARGTSFLKDRMGQRIMPAGIHIIDDPTQSRGLSAHPFDGEGMRAGELVIVQDGVLMNWALDSR
SARQVGLPGNGRASRGTTAPPGPSLGSLYARPGTVTPAALMADIAEGIYVTELMGSAINGLTGDYSRGASGFMIRGGTLA
EPVAELTIAGNLNDMFARMVLADDLVFRRGINAPTIRIDDLMIAGS

Sequences:

>Translated_446_residues
MSTDPLDLLGTLIGKARAAGADAADAVYMARTTHGVQVRNGRTEDLERSETSDLGLRVFVGRRSAIVSATSLDPERFDPL
VAQALAMARVVPEDPHSGLSPDAVQGFVDAAGMDLLDPAQPDTAALLDRARAAEDAAVSVAGVTNSNGGSASSGLSDIIL
MTSAGFSGRYARSSHSVSASVLAGQGTGMQRDYDYHATVHLSDLDDPAKIGRSAGEKAVARLNPTRPRTGSMAVVFDPRV
SSSLLGHLAGAVNGSAIARGTSFLKDRMGQRIMPAGIHIIDDPTQSRGLSAHPFDGEGMRAGELVIVQDGVLMNWALDSR
SARQVGLPGNGRASRGTTAPPGPSLGSLYARPGTVTPAALMADIAEGIYVTELMGSAINGLTGDYSRGASGFMIRGGTLA
EPVAELTIAGNLNDMFARMVLADDLVFRRGINAPTIRIDDLMIAGS
>Mature_445_residues
STDPLDLLGTLIGKARAAGADAADAVYMARTTHGVQVRNGRTEDLERSETSDLGLRVFVGRRSAIVSATSLDPERFDPLV
AQALAMARVVPEDPHSGLSPDAVQGFVDAAGMDLLDPAQPDTAALLDRARAAEDAAVSVAGVTNSNGGSASSGLSDIILM
TSAGFSGRYARSSHSVSASVLAGQGTGMQRDYDYHATVHLSDLDDPAKIGRSAGEKAVARLNPTRPRTGSMAVVFDPRVS
SSLLGHLAGAVNGSAIARGTSFLKDRMGQRIMPAGIHIIDDPTQSRGLSAHPFDGEGMRAGELVIVQDGVLMNWALDSRS
ARQVGLPGNGRASRGTTAPPGPSLGSLYARPGTVTPAALMADIAEGIYVTELMGSAINGLTGDYSRGASGFMIRGGTLAE
PVAELTIAGNLNDMFARMVLADDLVFRRGINAPTIRIDDLMIAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=431, Percent_Identity=34.5707656612529, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1789640, Length=267, Percent_Identity=23.9700374531835, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46177; Mature: 46045

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDPLDLLGTLIGKARAAGADAADAVYMARTTHGVQVRNGRTEDLERSETSDLGLRVFV
CCCCHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCEEECCCCCHHHHHCCCCCCCEEEEE
GRRSAIVSATSLDPERFDPLVAQALAMARVVPEDPHSGLSPDAVQGFVDAAGMDLLDPAQ
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
PDTAALLDRARAAEDAAVSVAGVTNSNGGSASSGLSDIILMTSAGFSGRYARSSHSVSAS
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
VLAGQGTGMQRDYDYHATVHLSDLDDPAKIGRSAGEKAVARLNPTRPRTGSMAVVFDPRV
EEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCH
SSSLLGHLAGAVNGSAIARGTSFLKDRMGQRIMPAGIHIIDDPTQSRGLSAHPFDGEGMR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEECCCCCEEECCCCHHCCCCCCCCCCCCCC
AGELVIVQDGVLMNWALDSRSARQVGLPGNGRASRGTTAPPGPSLGSLYARPGTVTPAAL
CCCEEEEECCEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
MADIAEGIYVTELMGSAINGLTGDYSRGASGFMIRGGTLAEPVAELTIAGNLNDMFARMV
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHCCHHHEEEECCHHHHHHHHH
LADDLVFRRGINAPTIRIDDLMIAGS
HHHHHHHHCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
STDPLDLLGTLIGKARAAGADAADAVYMARTTHGVQVRNGRTEDLERSETSDLGLRVFV
CCCHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCEEECCCCCHHHHHCCCCCCCEEEEE
GRRSAIVSATSLDPERFDPLVAQALAMARVVPEDPHSGLSPDAVQGFVDAAGMDLLDPAQ
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
PDTAALLDRARAAEDAAVSVAGVTNSNGGSASSGLSDIILMTSAGFSGRYARSSHSVSAS
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
VLAGQGTGMQRDYDYHATVHLSDLDDPAKIGRSAGEKAVARLNPTRPRTGSMAVVFDPRV
EEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCH
SSSLLGHLAGAVNGSAIARGTSFLKDRMGQRIMPAGIHIIDDPTQSRGLSAHPFDGEGMR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEECCCCCEEECCCCHHCCCCCCCCCCCCCC
AGELVIVQDGVLMNWALDSRSARQVGLPGNGRASRGTTAPPGPSLGSLYARPGTVTPAAL
CCCEEEEECCEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
MADIAEGIYVTELMGSAINGLTGDYSRGASGFMIRGGTLAEPVAELTIAGNLNDMFARMV
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHCCHHHEEEECCHHHHHHHHH
LADDLVFRRGINAPTIRIDDLMIAGS
HHHHHHHHCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]