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The map label for this gene is mdtN [H]

Identifier: 162147802

GI number: 162147802

Start: 2060681

End: 2062018

Strand: Reverse

Name: mdtN [H]

Synonym: GDI_2018

Alternate gene names: 162147802

Gene position: 2062018-2060681 (Counterclockwise)

Preceding gene: 162147803

Following gene: 162147801

Centisome position: 52.28

GC content: 66.44

Gene sequence:

>1338_bases
TTGCGGTTATTTCATGACCTCGCATTCCGCTCCTCTTGCGCGTGTAGCGCCCGTTCTTTTCCTCGGTGGCTGCGCTGTGC
GTGGCGCCCCGTCCTTTCCACTGGTCGGGGCCTATTTCCCGGGCTGGATGTTCTGCGCAATGGTGGGTATCGCCGCCGCC
GTGGGTTTGCGGGTGGTATTCATACAGACCGGTATCGACGCGATCCTGAGCTTCCGGCTGTTCACCTACGTATCGCTCGG
CGTGATCGCGGCGCTCCTGGCCTGGCTGATCTGGTTCGGGCCATAGCGATGCGAAGGGTTTCCATGTCCGGCAGGAAGCC
TGTCGGTCTCATCATCGCCGTGGCCTGCGTCGTGGCCGCTGTCCTGCTGGGCGTCCATGTCGCGAACGAGGACAGCGCCC
ATCCTTCCTCCGACAGCGGGTCGATCGACGCCGAAATCGTGCACATGGCTTCGACCGTCGGCGGCCGACTGGTCGATCTG
CCCGTTCACGTCAATCAGGCGGTGCGCAAGGGCGACCTGCTCTATCGTCTCGATCCCGAACCGTACGAGCTGACCGTGCG
GCAGGCGCAAGCCAATCTGGCGCTGGCCCAGGCCGAGGTCGAAAACCAGCGACGGCTGGTCTCGGTCAAGACCGCCGACG
CCGCCGTGGCACAGGCCCAGACCGCGCGCGCGCGAACCAACCGCGATCTCGCCGCGCGCACGGTCGAACGCCTCTCGCCT
CTGGCCGGGCATGCCTATATCCCGTTGCAGCAATATGACCAGGCCCGGGTTGCCCTCCGCGATGCCGAGATATCGCTGGA
GCAGGCGCATCGGCAGGAAGCGGCGGCACAGACCGCAATCGGAGACCTGAACAGTTCGCTGGCGGCGCGGGACGCCGCTG
CGGCGGCGCTCGATCACGCGCGCTATGAACTCAGGCAGACAACGGTCGTCGCCCCCCTGGACGGCTACGTGACCAGCCTG
CGCGTCAAGCCAGGCGAGATTCTGGCGCCGGCGCAGGTGCTGTTCACCCTGATCGCCAACGATGCATGGTATGCCAACGC
CAATATCCGGGAGGTCGATCTGCGCCCCATCCGGCCCGGAGACTGCGCGACAGTCCATTCCATGATCGACCGGCACGTGG
CCATTCGCGGTCATGTGGACAGCATCGGCTGGGGCGTCCTGTCCGCCGATTCGGCAGGGATCGCACGCGCCCTTCCCGTC
GTGCCGCGCGAAATGGATTGGGTGCACGTGGCGCAGCGGTTCCCCGTGCGGGTAAAGCTCGATCGGGCCGACCCGAACCT
GCTGCGCCTGGGTGCCACCGCCACCGTCGAGATCCGGCATGGCGCCGCCTGTCCCTGA

Upstream 100 bases:

>100_bases
CGGGCGGCGGGCCCGGATGCGTCGCGACCGGAGATGGACCTGAGATTTCTGATACGTTAGGGTAACGCAATAGCCGTCAG
CCGTCTTAACGCGTGGGGAT

Downstream 100 bases:

>100_bases
CCGACATATCGCCGATCCCGGGCGCCTGACGCTCGGGCGGGCCTGGAGGCTGGCGTGCGACCCGGAACCGGGGCGTCTCG
GCTACGCCCTGCGCATGGCG

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MRLFHDLAFRSSCACSARSFPRWLRCAWRPVLSTGRGLFPGLDVLRNGGYRRRRGFAGGIHTDRYRRDPELPAVHLRIAR
RDRGAPGLADLVRAIAMRRVSMSGRKPVGLIIAVACVVAAVLLGVHVANEDSAHPSSDSGSIDAEIVHMASTVGGRLVDL
PVHVNQAVRKGDLLYRLDPEPYELTVRQAQANLALAQAEVENQRRLVSVKTADAAVAQAQTARARTNRDLAARTVERLSP
LAGHAYIPLQQYDQARVALRDAEISLEQAHRQEAAAQTAIGDLNSSLAARDAAAAALDHARYELRQTTVVAPLDGYVTSL
RVKPGEILAPAQVLFTLIANDAWYANANIREVDLRPIRPGDCATVHSMIDRHVAIRGHVDSIGWGVLSADSAGIARALPV
VPREMDWVHVAQRFPVRVKLDRADPNLLRLGATATVEIRHGAACP

Sequences:

>Translated_445_residues
MRLFHDLAFRSSCACSARSFPRWLRCAWRPVLSTGRGLFPGLDVLRNGGYRRRRGFAGGIHTDRYRRDPELPAVHLRIAR
RDRGAPGLADLVRAIAMRRVSMSGRKPVGLIIAVACVVAAVLLGVHVANEDSAHPSSDSGSIDAEIVHMASTVGGRLVDL
PVHVNQAVRKGDLLYRLDPEPYELTVRQAQANLALAQAEVENQRRLVSVKTADAAVAQAQTARARTNRDLAARTVERLSP
LAGHAYIPLQQYDQARVALRDAEISLEQAHRQEAAAQTAIGDLNSSLAARDAAAAALDHARYELRQTTVVAPLDGYVTSL
RVKPGEILAPAQVLFTLIANDAWYANANIREVDLRPIRPGDCATVHSMIDRHVAIRGHVDSIGWGVLSADSAGIARALPV
VPREMDWVHVAQRFPVRVKLDRADPNLLRLGATATVEIRHGAACP
>Mature_445_residues
MRLFHDLAFRSSCACSARSFPRWLRCAWRPVLSTGRGLFPGLDVLRNGGYRRRRGFAGGIHTDRYRRDPELPAVHLRIAR
RDRGAPGLADLVRAIAMRRVSMSGRKPVGLIIAVACVVAAVLLGVHVANEDSAHPSSDSGSIDAEIVHMASTVGGRLVDL
PVHVNQAVRKGDLLYRLDPEPYELTVRQAQANLALAQAEVENQRRLVSVKTADAAVAQAQTARARTNRDLAARTVERLSP
LAGHAYIPLQQYDQARVALRDAEISLEQAHRQEAAAQTAIGDLNSSLAARDAAAAALDHARYELRQTTVVAPLDGYVTSL
RVKPGEILAPAQVLFTLIANDAWYANANIREVDLRPIRPGDCATVHSMIDRHVAIRGHVDSIGWGVLSADSAGIARALPV
VPREMDWVHVAQRFPVRVKLDRADPNLLRLGATATVEIRHGAACP

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=309, Percent_Identity=37.5404530744337, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789637, Length=325, Percent_Identity=27.0769230769231, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081951, Length=336, Percent_Identity=28.2738095238095, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789041, Length=335, Percent_Identity=26.865671641791, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788711, Length=281, Percent_Identity=25.9786476868327, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790024, Length=281, Percent_Identity=26.6903914590747, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790012, Length=220, Percent_Identity=29.0909090909091, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787013, Length=225, Percent_Identity=28.4444444444444, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48443; Mature: 48443

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFHDLAFRSSCACSARSFPRWLRCAWRPVLSTGRGLFPGLDVLRNGGYRRRRGFAGGI
CCHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCCCCCCC
HTDRYRRDPELPAVHLRIARRDRGAPGLADLVRAIAMRRVSMSGRKPVGLIIAVACVVAA
CCHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VLLGVHVANEDSAHPSSDSGSIDAEIVHMASTVGGRLVDLPVHVNQAVRKGDLLYRLDPE
HHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHCCCEEEEECCC
PYELTVRQAQANLALAQAEVENQRRLVSVKTADAAVAQAQTARARTNRDLAARTVERLSP
CCEEEEEHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHCH
LAGHAYIPLQQYDQARVALRDAEISLEQAHRQEAAAQTAIGDLNSSLAARDAAAAALDHA
HCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYELRQTTVVAPLDGYVTSLRVKPGEILAPAQVLFTLIANDAWYANANIREVDLRPIRPG
HHHHHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCEEEEECCCCCCC
DCATVHSMIDRHVAIRGHVDSIGWGVLSADSAGIARALPVVPREMDWVHVAQRFPVRVKL
CHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCHHHHCCCCCCCCHHHHHHHHCCEEEEE
DRADPNLLRLGATATVEIRHGAACP
ECCCCCEEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MRLFHDLAFRSSCACSARSFPRWLRCAWRPVLSTGRGLFPGLDVLRNGGYRRRRGFAGGI
CCHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCCCCCCC
HTDRYRRDPELPAVHLRIARRDRGAPGLADLVRAIAMRRVSMSGRKPVGLIIAVACVVAA
CCHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VLLGVHVANEDSAHPSSDSGSIDAEIVHMASTVGGRLVDLPVHVNQAVRKGDLLYRLDPE
HHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHCCCEEEEECCC
PYELTVRQAQANLALAQAEVENQRRLVSVKTADAAVAQAQTARARTNRDLAARTVERLSP
CCEEEEEHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHCH
LAGHAYIPLQQYDQARVALRDAEISLEQAHRQEAAAQTAIGDLNSSLAARDAAAAALDHA
HCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYELRQTTVVAPLDGYVTSLRVKPGEILAPAQVLFTLIANDAWYANANIREVDLRPIRPG
HHHHHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCEEEEECCCCCCC
DCATVHSMIDRHVAIRGHVDSIGWGVLSADSAGIARALPVVPREMDWVHVAQRFPVRVKL
CHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCHHHHCCCCCCCCHHHHHHHHCCEEEEE
DRADPNLLRLGATATVEIRHGAACP
ECCCCCEEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]