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The map label for this gene is mdtO [H]

Identifier: 162147801

GI number: 162147801

Start: 2058898

End: 2060655

Strand: Reverse

Name: mdtO [H]

Synonym: GDI_2017

Alternate gene names: 162147801

Gene position: 2060655-2058898 (Counterclockwise)

Preceding gene: 162147802

Following gene: 162147800

Centisome position: 52.25

GC content: 65.42

Gene sequence:

>1758_bases
CTGACGCTCGGGCGGGCCTGGAGGCTGGCGTGCGACCCGGAACCGGGGCGTCTCGGCTACGCCCTGCGCATGGCGGCAGC
CTGCGCCACGACGGTCCTAGTGGGGGAAATCTGGCAGGTCCCCGACCTCGCCGTGCCCGCCCTGGTCACGATGGCGCTCT
GGCAGAAGGACCGCGTGACCAATCTCGTCGCCGGCCTCGCCGTGAATGTGCTGATCCTCGTCATCCTCGCCATGCTGTAC
GGGCTGATTCGCCTGACGCTCGATCATCCGCTGGGCATCGTCGTCGGCGTGGCGATATTGTCCTTCTGCTTCTTCTTCCT
CGGGTCAGCCAGCAAGCTCAAGCCGCTCGCCTATATGCTGGGGCTGATCACCGTCTATGGGCTGATCGCCGTCGATCAGG
CGCCGGTCGGCGAGATCGCCACCCGCGCGCTGCTCTACACCGATCTGTTCCTCGCCATACCGGGCCTGGTCATGATCGGG
CTCGGACTGATCATCTGCCCGTCGCCCAGGACCGTGCTGATGCGCGCCATCGCAGGCCGCCTGCGCATGGCCATGGCGCT
GTTGCGTCATCCCGATGACGCGACGCGCGAGCGCGCCCAGGACATGCTGCGCCAGGGCGGCGCGGGCATGATGGCGAACG
TGAAGATGGCGGCGCTGGAAAGGATCTGGAACCGGCGCGACCTCGCCTGCCTGAAGCAGGCGGCCTATTCCAGCATCGGC
GTCCTTGCCCTGGCCGACGCGGCGGTGCGAGAAGGCGGATCACCTTGCCCGTCTGCCCTGCTGGAAACATTGGAGGAGAT
GGCGGCGATGTTCGAGGCGGGCGATTATCCCGCCGATATCGCGCCCCCCGCCGTGCCCTCCAACGAACCGGCCCTTGCCG
CAATGGCGGCACTGATCGCGATTTTCACGACCCCGGCACCGGAGGAAAAGCCCCCGGAGGAGAAAAAAAGCGGGGGCTTT
TTTTTCCCCGACGCCTTCACGAACCCCGACCATGTCCGCTTTGCCGTGAAGGGCACGGCCGCGGTGATGACCAGCTATTT
CATCTTCAAGATCCTCGACTGGCCGGGCATCCATACCTGCATCATCACGTGTTTCATCGTGGCCCAGCCGACGATGGGCG
AGATGATTTCCAAGCTCATGCTGCGTATCGGCGGCGCACTGCTGGGTGCCGCGCTCGGCATCGGCTCGATCATCCTGCTG
ATGCCGCATCTGAACGATATCACCGGGTTCCTGGCTCTTGTCTTCGCGGGCGCGTTCATCGCAGCCTGGGTCAAGACCGG
TGACGAACGCATTGCGTATGCGGGATTCCAGATCGGGCTGGCCTTCTTCCTGACGGACATCAAGGATTACGGCCCGACCA
CCGACATGACCGTGGCGCGCGACCGCGTGGTCGGCATCATGCTCGGCAATTTCATCACCTATGCGATGTTCACCAGTTTC
TGGCCCGCCAGCGCCTGGGACAGGATCGCGGCGACGTTGCGGGGCGTCGTCAGGGCCCTTGCGGCACAGCGCGACAGTAT
CACGCCGCAGGCGCGCGTGACCCATGCGGCCGAGGTGTTGGGCGCGATCTCGGCCAGCCAGCGCACGCTTGAATTCGCGG
CCACCGAACCCGTCCATATGCGCGCCGATACGGAAAGGATGTCCCAATGCGGCGAAGCCCTGCGGGATGCCTCCCGGCTG
GCCGAAGACCTGCTGATTCCCGAACGCGATGCCCAGACGCGCGCACGTTTCGAACGGTTGGAGAGCCTGGCCTCGTGA

Upstream 100 bases:

>100_bases
CGGGCCGACCCGAACCTGCTGCGCCTGGGTGCCACCGCCACCGTCGAGATCCGGCATGGCGCCGCCTGTCCCTGACCGAC
ATATCGCCGATCCCGGGCGC

Downstream 100 bases:

>100_bases
CCCGTCCCGCCTCCCTCGCAACCATCGGGTTCGCCATGCTCGGGCTGGCCGGTTGCGCCACCCAAGCCCTGCATTCGGCG
CCGGACCGGCCCGACGCGCC

Product: multidrug resistance protein mdtO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTLGRAWRLACDPEPGRLGYALRMAAACATTVLVGEIWQVPDLAVPALVTMALWQKDRVTNLVAGLAVNVLILVILAMLY
GLIRLTLDHPLGIVVGVAILSFCFFFLGSASKLKPLAYMLGLITVYGLIAVDQAPVGEIATRALLYTDLFLAIPGLVMIG
LGLIICPSPRTVLMRAIAGRLRMAMALLRHPDDATRERAQDMLRQGGAGMMANVKMAALERIWNRRDLACLKQAAYSSIG
VLALADAAVREGGSPCPSALLETLEEMAAMFEAGDYPADIAPPAVPSNEPALAAMAALIAIFTTPAPEEKPPEEKKSGGF
FFPDAFTNPDHVRFAVKGTAAVMTSYFIFKILDWPGIHTCIITCFIVAQPTMGEMISKLMLRIGGALLGAALGIGSIILL
MPHLNDITGFLALVFAGAFIAAWVKTGDERIAYAGFQIGLAFFLTDIKDYGPTTDMTVARDRVVGIMLGNFITYAMFTSF
WPASAWDRIAATLRGVVRALAAQRDSITPQARVTHAAEVLGAISASQRTLEFAATEPVHMRADTERMSQCGEALRDASRL
AEDLLIPERDAQTRARFERLESLAS

Sequences:

>Translated_585_residues
MTLGRAWRLACDPEPGRLGYALRMAAACATTVLVGEIWQVPDLAVPALVTMALWQKDRVTNLVAGLAVNVLILVILAMLY
GLIRLTLDHPLGIVVGVAILSFCFFFLGSASKLKPLAYMLGLITVYGLIAVDQAPVGEIATRALLYTDLFLAIPGLVMIG
LGLIICPSPRTVLMRAIAGRLRMAMALLRHPDDATRERAQDMLRQGGAGMMANVKMAALERIWNRRDLACLKQAAYSSIG
VLALADAAVREGGSPCPSALLETLEEMAAMFEAGDYPADIAPPAVPSNEPALAAMAALIAIFTTPAPEEKPPEEKKSGGF
FFPDAFTNPDHVRFAVKGTAAVMTSYFIFKILDWPGIHTCIITCFIVAQPTMGEMISKLMLRIGGALLGAALGIGSIILL
MPHLNDITGFLALVFAGAFIAAWVKTGDERIAYAGFQIGLAFFLTDIKDYGPTTDMTVARDRVVGIMLGNFITYAMFTSF
WPASAWDRIAATLRGVVRALAAQRDSITPQARVTHAAEVLGAISASQRTLEFAATEPVHMRADTERMSQCGEALRDASRL
AEDLLIPERDAQTRARFERLESLAS
>Mature_584_residues
TLGRAWRLACDPEPGRLGYALRMAAACATTVLVGEIWQVPDLAVPALVTMALWQKDRVTNLVAGLAVNVLILVILAMLYG
LIRLTLDHPLGIVVGVAILSFCFFFLGSASKLKPLAYMLGLITVYGLIAVDQAPVGEIATRALLYTDLFLAIPGLVMIGL
GLIICPSPRTVLMRAIAGRLRMAMALLRHPDDATRERAQDMLRQGGAGMMANVKMAALERIWNRRDLACLKQAAYSSIGV
LALADAAVREGGSPCPSALLETLEEMAAMFEAGDYPADIAPPAVPSNEPALAAMAALIAIFTTPAPEEKPPEEKKSGGFF
FPDAFTNPDHVRFAVKGTAAVMTSYFIFKILDWPGIHTCIITCFIVAQPTMGEMISKLMLRIGGALLGAALGIGSIILLM
PHLNDITGFLALVFAGAFIAAWVKTGDERIAYAGFQIGLAFFLTDIKDYGPTTDMTVARDRVVGIMLGNFITYAMFTSFW
PASAWDRIAATLRGVVRALAAQRDSITPQARVTHAAEVLGAISASQRTLEFAATEPVHMRADTERMSQCGEALRDASRLA
EDLLIPERDAQTRARFERLESLAS

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1289

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MdtO family [H]

Homologues:

Organism=Escherichia coli, GI87082367, Length=485, Percent_Identity=27.0103092783505, Blast_Score=146, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006726 [H]

Pfam domain/function: PF04632 FUSC [H]

EC number: NA

Molecular weight: Translated: 62972; Mature: 62841

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGRAWRLACDPEPGRLGYALRMAAACATTVLVGEIWQVPDLAVPALVTMALWQKDRVT
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NLVAGLAVNVLILVILAMLYGLIRLTLDHPLGIVVGVAILSFCFFFLGSASKLKPLAYML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GLITVYGLIAVDQAPVGEIATRALLYTDLFLAIPGLVMIGLGLIICPSPRTVLMRAIAGR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHH
LRMAMALLRHPDDATRERAQDMLRQGGAGMMANVKMAALERIWNRRDLACLKQAAYSSIG
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VLALADAAVREGGSPCPSALLETLEEMAAMFEAGDYPADIAPPAVPSNEPALAAMAALIA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
IFTTPAPEEKPPEEKKSGGFFFPDAFTNPDHVRFAVKGTAAVMTSYFIFKILDWPGIHTC
HHCCCCCCCCCCCHHCCCCEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHH
IITCFIVAQPTMGEMISKLMLRIGGALLGAALGIGSIILLMPHLNDITGFLALVFAGAFI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AAWVKTGDERIAYAGFQIGLAFFLTDIKDYGPTTDMTVARDRVVGIMLGNFITYAMFTSF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
WPASAWDRIAATLRGVVRALAAQRDSITPQARVTHAAEVLGAISASQRTLEFAATEPVHM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
RADTERMSQCGEALRDASRLAEDLLIPERDAQTRARFERLESLAS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLGRAWRLACDPEPGRLGYALRMAAACATTVLVGEIWQVPDLAVPALVTMALWQKDRVT
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NLVAGLAVNVLILVILAMLYGLIRLTLDHPLGIVVGVAILSFCFFFLGSASKLKPLAYML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GLITVYGLIAVDQAPVGEIATRALLYTDLFLAIPGLVMIGLGLIICPSPRTVLMRAIAGR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHH
LRMAMALLRHPDDATRERAQDMLRQGGAGMMANVKMAALERIWNRRDLACLKQAAYSSIG
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VLALADAAVREGGSPCPSALLETLEEMAAMFEAGDYPADIAPPAVPSNEPALAAMAALIA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
IFTTPAPEEKPPEEKKSGGFFFPDAFTNPDHVRFAVKGTAAVMTSYFIFKILDWPGIHTC
HHCCCCCCCCCCCHHCCCCEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHH
IITCFIVAQPTMGEMISKLMLRIGGALLGAALGIGSIILLMPHLNDITGFLALVFAGAFI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AAWVKTGDERIAYAGFQIGLAFFLTDIKDYGPTTDMTVARDRVVGIMLGNFITYAMFTSF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
WPASAWDRIAATLRGVVRALAAQRDSITPQARVTHAAEVLGAISASQRTLEFAATEPVHM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
RADTERMSQCGEALRDASRLAEDLLIPERDAQTRARFERLESLAS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]