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The map label for this gene is leuB [H]

Identifier: 162147775

GI number: 162147775

Start: 2025329

End: 2026357

Strand: Direct

Name: leuB [H]

Synonym: GDI_1991

Alternate gene names: 162147775

Gene position: 2025329-2026357 (Clockwise)

Preceding gene: 162147774

Following gene: 162147789

Centisome position: 51.35

GC content: 66.28

Gene sequence:

>1029_bases
ATGTCCGCTGAAAAGACCATTCCGGCTACCCTGATCCCCGGCGATGGGATCGGACCCGAAATCACGGAATCCGTGACCGA
GATCCTGGCGGCCGTCGGCGCGCCGTTCGACTGGGACAACCAGCTCGCGGGCATGGCGGCGGTCGACGCCACCGGCAATC
CGCTGCCGCCGCAGACGATCGCCAGCATCCATCGCACCGGACTGGCCCTGAAGGGCCCGCTGACCACCCCGGTCGGCGGC
GGCTTCAAGTCGATCAACGTCACGCTGCGGCAGGAGTTCGGCCTGTTCGCCAATCTTCGTCCGACGCACACGATCGTCCC
CGGCGGCCGCTTCGACAAGATCGACCTGGTCCTGGTGCGCGAGAACCTGGAAGGCTACTACGCCGCCATGGAAAGCTACA
TCCCGGTGGGCGACGACCCGCACGGGATCGCGACCGGCGCCGGCTACAACTCGCGCGCCGAATGCAACCGCATTGTCCGC
TTCGCCTTCGAATACGCGGTCAAGAACAACCGCAAGAAGGTGACGCTGGTCCACAAGGCCAACATCCTGAAGCTGCTGAC
CGGCCTATTCCTGGAAGAAGGCCGCAAGGTCGCCAAGGAATATGAAGGCCGCGTCGAGTGCAACGAGCGCATCGTCGATG
CGTGCGCCATGCAGCTGGTCATCGACCCGTGGCAGTTCGACGTGATCGTCACCACGAACCTGTTCGGCGACATCCTGTCC
GACCTGACGGCCGGCCTGGTCGGCGGCCTGGGCCTGGCCCCGGGCGCCAATATCGGCGAGAAGGCAGCGGTATTCGAGGC
CGTTCACGGCTCGGCGCCCGACATCGCCGGCAAGAACGTCGCCAACCCGCTGGCGCTGCTGATGGCCGCGAACATGATGC
TGAAGCATGTCGGCCGCGCGGATCTGGCCACCCGGATCGAAAGCGCGATCCAGTCCGTCATCACAGAGGGCACGGTCCGC
ACCCGCGACCTGGGCGGCACCGCCGGCACGCGCGAACTGACCGCGGCGCTGAAGCAGAAGCTGGTCTGA

Upstream 100 bases:

>100_bases
GACACTGGGACCATACGGGTTCCGGCATGGCGGTTCCGCCTCGCGAACGGATCGTCCGTGGCCCGGACCTGTCAGACTGC
ACAGAGGGAGACGTTCGACA

Downstream 100 bases:

>100_bases
CCCGGAATGCCGGCCCGGTTTTCCCGGGCCGGCAACCTCTCTCAGCTTCCCCGATAGGTCGAAAACCCGTACGGGGAGAC
CAGCAGCGGCACATGATAGT

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSAEKTIPATLIPGDGIGPEITESVTEILAAVGAPFDWDNQLAGMAAVDATGNPLPPQTIASIHRTGLALKGPLTTPVGG
GFKSINVTLRQEFGLFANLRPTHTIVPGGRFDKIDLVLVRENLEGYYAAMESYIPVGDDPHGIATGAGYNSRAECNRIVR
FAFEYAVKNNRKKVTLVHKANILKLLTGLFLEEGRKVAKEYEGRVECNERIVDACAMQLVIDPWQFDVIVTTNLFGDILS
DLTAGLVGGLGLAPGANIGEKAAVFEAVHGSAPDIAGKNVANPLALLMAANMMLKHVGRADLATRIESAIQSVITEGTVR
TRDLGGTAGTRELTAALKQKLV

Sequences:

>Translated_342_residues
MSAEKTIPATLIPGDGIGPEITESVTEILAAVGAPFDWDNQLAGMAAVDATGNPLPPQTIASIHRTGLALKGPLTTPVGG
GFKSINVTLRQEFGLFANLRPTHTIVPGGRFDKIDLVLVRENLEGYYAAMESYIPVGDDPHGIATGAGYNSRAECNRIVR
FAFEYAVKNNRKKVTLVHKANILKLLTGLFLEEGRKVAKEYEGRVECNERIVDACAMQLVIDPWQFDVIVTTNLFGDILS
DLTAGLVGGLGLAPGANIGEKAAVFEAVHGSAPDIAGKNVANPLALLMAANMMLKHVGRADLATRIESAIQSVITEGTVR
TRDLGGTAGTRELTAALKQKLV
>Mature_341_residues
SAEKTIPATLIPGDGIGPEITESVTEILAAVGAPFDWDNQLAGMAAVDATGNPLPPQTIASIHRTGLALKGPLTTPVGGG
FKSINVTLRQEFGLFANLRPTHTIVPGGRFDKIDLVLVRENLEGYYAAMESYIPVGDDPHGIATGAGYNSRAECNRIVRF
AFEYAVKNNRKKVTLVHKANILKLLTGLFLEEGRKVAKEYEGRVECNERIVDACAMQLVIDPWQFDVIVTTNLFGDILSD
LTAGLVGGLGLAPGANIGEKAAVFEAVHGSAPDIAGKNVANPLALLMAANMMLKHVGRADLATRIESAIQSVITEGTVRT
RDLGGTAGTRELTAALKQKLV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=337, Percent_Identity=43.9169139465875, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI28178816, Length=340, Percent_Identity=38.5294117647059, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI28178821, Length=340, Percent_Identity=38.2352941176471, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=38.728323699422, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI28178838, Length=334, Percent_Identity=38.9221556886228, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI28178819, Length=189, Percent_Identity=46.031746031746, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1787381, Length=358, Percent_Identity=35.7541899441341, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI87081683, Length=349, Percent_Identity=36.3896848137536, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=32.5842696629214, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71986051, Length=329, Percent_Identity=45.2887537993921, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI25144293, Length=333, Percent_Identity=40.5405405405405, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17550882, Length=346, Percent_Identity=36.7052023121387, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=38.8059701492537, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324709, Length=339, Percent_Identity=42.4778761061947, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324291, Length=357, Percent_Identity=38.375350140056, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=37.5350140056022, Blast_Score=208, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6319830, Length=371, Percent_Identity=32.3450134770889, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24643270, Length=345, Percent_Identity=44.0579710144928, Blast_Score=277, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24643268, Length=345, Percent_Identity=44.0579710144928, Blast_Score=277, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24661184, Length=346, Percent_Identity=41.0404624277457, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI161078635, Length=331, Percent_Identity=40.1812688821752, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI161078637, Length=331, Percent_Identity=40.1812688821752, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI161078633, Length=331, Percent_Identity=40.1812688821752, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24650122, Length=331, Percent_Identity=40.1812688821752, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI161078639, Length=338, Percent_Identity=39.3491124260355, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI281362242, Length=336, Percent_Identity=39.2857142857143, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24648872, Length=336, Percent_Identity=39.2857142857143, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI20130355, Length=341, Percent_Identity=31.9648093841642, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI45550436, Length=219, Percent_Identity=26.027397260274, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 8180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36278; Mature: 36147

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEKTIPATLIPGDGIGPEITESVTEILAAVGAPFDWDNQLAGMAAVDATGNPLPPQTI
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHH
ASIHRTGLALKGPLTTPVGGGFKSINVTLRQEFGLFANLRPTHTIVPGGRFDKIDLVLVR
HHHHHCCCEEECCCCCCCCCCCEEEEEEEEHHCCCEECCCCCEEECCCCCCCCEEEEEEE
ENLEGYYAAMESYIPVGDDPHGIATGAGYNSRAECNRIVRFAFEYAVKNNRKKVTLVHKA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHH
NILKLLTGLFLEEGRKVAKEYEGRVECNERIVDACAMQLVIDPWQFDVIVTTNLFGDILS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
DLTAGLVGGLGLAPGANIGEKAAVFEAVHGSAPDIAGKNVANPLALLMAANMMLKHVGRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
DLATRIESAIQSVITEGTVRTRDLGGTAGTRELTAALKQKLV
HHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SAEKTIPATLIPGDGIGPEITESVTEILAAVGAPFDWDNQLAGMAAVDATGNPLPPQTI
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHH
ASIHRTGLALKGPLTTPVGGGFKSINVTLRQEFGLFANLRPTHTIVPGGRFDKIDLVLVR
HHHHHCCCEEECCCCCCCCCCCEEEEEEEEHHCCCEECCCCCEEECCCCCCCCEEEEEEE
ENLEGYYAAMESYIPVGDDPHGIATGAGYNSRAECNRIVRFAFEYAVKNNRKKVTLVHKA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHH
NILKLLTGLFLEEGRKVAKEYEGRVECNERIVDACAMQLVIDPWQFDVIVTTNLFGDILS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
DLTAGLVGGLGLAPGANIGEKAAVFEAVHGSAPDIAGKNVANPLALLMAANMMLKHVGRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
DLATRIESAIQSVITEGTVRTRDLGGTAGTRELTAALKQKLV
HHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]