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The map label for this gene is lexA

Identifier: 162147707

GI number: 162147707

Start: 1957792

End: 1958553

Strand: Reverse

Name: lexA

Synonym: GDI_1923

Alternate gene names: 162147707

Gene position: 1958553-1957792 (Counterclockwise)

Preceding gene: 162147708

Following gene: 162147703

Centisome position: 49.66

GC content: 66.54

Gene sequence:

>762_bases
ATGCTGACGCGCAAGCAACATGAACTGCTGCTGTTCATCGACCGGCATCTGAAACAGACGGGCTTTTCACCCTCCTTCGA
CGAAATGAAGGATGCGCTGAACCTGCGGTCGAAATCCGGCATCCACCGCCTGATTTCGGCGCTGGAGGAGCGGGATTTCC
TCCGTCGCCGTCACCACCGCGCCCGCGCCCTGGAAGTCCTGCGCCTGCCGGAAACCATGCCGGCCGCCACCGGCAAGCCG
CCCCTGGCGGAATCCGGTCCCCCGCCGGTCACCGCACCCGCCACCGACGAGTCCGCTGCTGCCGAATCCTTCGTGCCCAA
TGTCATCAAGGGTGATTTCGCCAACCGGCTGGCGGGGGCGTCCGTTGCGACCGAGGCCGGGGCGATTCACCTGCCCTTCT
ATGGTCGGATCGCCGCCGGCCAGCCGATCGAGGCCCTGCGTGAAACCGGGGCGCAGATCGAGGTTCCGATGAACCTGCTG
GGCCATGGCGAGCATTATGCGCTGGAAGTCGCGGGCGATTCGATGATCGAGGCCGGAATCCTGGACGGCGATACGGTGAT
CATCCGCCGGGGCGACGTGGCGCAGAACGGACAGATCGTCGTCGCGCTGATCGACGACCAGGAAGTCACGCTGAAACGGC
TGCGCCGGCGCGGCAGCACCATCGCGCTGGAACCGGCCAACGCGCGCTACGAGCCGCGCATTGTCCCGTCGGACCGGGTC
CGAATCCAGGGCCAGCTCGTCGGCCTGCTCCGCCGGTATTGA

Upstream 100 bases:

>100_bases
GGGGAACCAATATAGAACATCGGCATTAGGTTTCCTGTTTGTTCCATGCCCTGGTAACGGATCGGCCAACCATCGGAACA
GTACGATCACGGGGGTGCCA

Downstream 100 bases:

>100_bases
CCGGGACGGACGGGTTTCAGGGATAGCACCACAGATCGTCATAGGACGGCAGCAAGCCGGCCAGGTTCGCGACCTGTTCG
GGACCGACCTGATCCAGATG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLTRKQHELLLFIDRHLKQTGFSPSFDEMKDALNLRSKSGIHRLISALEERDFLRRRHHRARALEVLRLPETMPAATGKP
PLAESGPPPVTAPATDESAAAESFVPNVIKGDFANRLAGASVATEAGAIHLPFYGRIAAGQPIEALRETGAQIEVPMNLL
GHGEHYALEVAGDSMIEAGILDGDTVIIRRGDVAQNGQIVVALIDDQEVTLKRLRRRGSTIALEPANARYEPRIVPSDRV
RIQGQLVGLLRRY

Sequences:

>Translated_253_residues
MLTRKQHELLLFIDRHLKQTGFSPSFDEMKDALNLRSKSGIHRLISALEERDFLRRRHHRARALEVLRLPETMPAATGKP
PLAESGPPPVTAPATDESAAAESFVPNVIKGDFANRLAGASVATEAGAIHLPFYGRIAAGQPIEALRETGAQIEVPMNLL
GHGEHYALEVAGDSMIEAGILDGDTVIIRRGDVAQNGQIVVALIDDQEVTLKRLRRRGSTIALEPANARYEPRIVPSDRV
RIQGQLVGLLRRY
>Mature_253_residues
MLTRKQHELLLFIDRHLKQTGFSPSFDEMKDALNLRSKSGIHRLISALEERDFLRRRHHRARALEVLRLPETMPAATGKP
PLAESGPPPVTAPATDESAAAESFVPNVIKGDFANRLAGASVATEAGAIHLPFYGRIAAGQPIEALRETGAQIEVPMNLL
GHGEHYALEVAGDSMIEAGILDGDTVIIRRGDVAQNGQIVVALIDDQEVTLKRLRRRGSTIALEPANARYEPRIVPSDRV
RIQGQLVGLLRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=123, Percent_Identity=38.2113821138211, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_GLUDA (A9HJ64)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602168.1
- RefSeq:   YP_002274560.1
- ProteinModelPortal:   A9HJ64
- SMR:   A9HJ64
- MEROPS:   S24.001
- GeneID:   5790177
- GeneID:   6973537
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0145
- HOGENOM:   HBG679610
- OMA:   LEPANAR
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 27763; Mature: 27763

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: ACT_SITE 174-174 ACT_SITE 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQHELLLFIDRHLKQTGFSPSFDEMKDALNLRSKSGIHRLISALEERDFLRRRHHR
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARALEVLRLPETMPAATGKPPLAESGPPPVTAPATDESAAAESFVPNVIKGDFANRLAGA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCC
SVATEAGAIHLPFYGRIAAGQPIEALRETGAQIEVPMNLLGHGEHYALEVAGDSMIEAGI
HHHHCCCEEECCCCCCCCCCCCHHHHHHCCCEEEECHHHHCCCCEEEEEECCCCHHHCCC
LDGDTVIIRRGDVAQNGQIVVALIDDQEVTLKRLRRRGSTIALEPANARYEPRIVPSDRV
CCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEECCCCCCCCCEECCCCCE
RIQGQLVGLLRRY
EEHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQHELLLFIDRHLKQTGFSPSFDEMKDALNLRSKSGIHRLISALEERDFLRRRHHR
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARALEVLRLPETMPAATGKPPLAESGPPPVTAPATDESAAAESFVPNVIKGDFANRLAGA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCC
SVATEAGAIHLPFYGRIAAGQPIEALRETGAQIEVPMNLLGHGEHYALEVAGDSMIEAGI
HHHHCCCEEECCCCCCCCCCCCHHHHHHCCCEEEECHHHHCCCCEEEEEECCCCHHHCCC
LDGDTVIIRRGDVAQNGQIVVALIDDQEVTLKRLRRRGSTIALEPANARYEPRIVPSDRV
CCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEECCCCCCCCCEECCCCCE
RIQGQLVGLLRRY
EEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA