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The map label for this gene is hemK [H]

Identifier: 162147701

GI number: 162147701

Start: 1951531

End: 1952412

Strand: Direct

Name: hemK [H]

Synonym: GDI_1917

Alternate gene names: 162147701

Gene position: 1951531-1952412 (Clockwise)

Preceding gene: 162147699

Following gene: 162147704

Centisome position: 49.48

GC content: 70.07

Gene sequence:

>882_bases
ATGAAGAGGTTCGACGCCGCCCCGCCCCACGATCCGGCGGATATCGCATCCCTTCTGGCGTGGGCGGCCGGCCGGCTGGC
CGCGGCCGGCCTGGATCATCCGCGACGCGAGGCCCGGCTGCTGATGGCGCACGTCCTGCGCACCGACCTGGCCGGCCTGC
TGGCCAGATCGGCGATGGACGCGGCCGAGCACCGATCCTTCGTGGCCCTGGTCGCGCGGCGGGCCGCACATGAACCGATG
GCCTATATCACCGGTCGGGCCGGATTCTGGTCGCTGGACCTCGAGACCGCGCCCGCCACGCTGATCCCCCGTGCCGACAG
CGAAACCCTGGTCGAGGCCCTGCTGGCCCAGCGGCCCGACCGGGGCGCGGTGCGCACGATCCTGGACCTGGGAACGGGGA
CGGGGTGCCTGCTGCTGGCGGCACTGAGCGAGTATCCCGACGCCTGGGGCCTGGGCGTGGATATCGACCCGGATGCCGCG
CATCTGGCCGCGCGCAATGCGCGCCGCACCGGGCTGCGGGACCGGTGCGCAATGCTGGCGGCCGACTGGTCCACGGCGAT
CGCGGGCCGTTTCGACGTGGTGTTCAGCAACCCGCCCTATATTCCCCGGGCCGATCTGGCGGGATTGATGCCGGATGTCC
GTGATCACGAGCCGGCCCGCGCCCTGGATGGCGGGACGGACGGGCTGGATGCCTACCGGCTGCTGACCGGGGCCTTGCCC
TCGCTTCTGGCGCATGGCGGCATTGCAATTTTTGAAATCGGCATCGGCCAGGAACGCAGCATGCCGGACCTCGCCCGACA
GGCGGGACTTGATATCGTGGGAATCCGTACCGATCTGGGTGGTATTCCCCGTGCTGTACTGATGCAACAGTGCCGGGTCT
GA

Upstream 100 bases:

>100_bases
CGGGCGCCGCCACGGTGGCGCAAGCCACCAGAATCGCAAGGATTCCCCATGAACGGACCTGATATGCCATGGCGGTACCG
TAGGATGGATTGCCGTTGAA

Downstream 100 bases:

>100_bases
GCCAAAAAACCATTTGGCAGACCGTGTACAAGGGAATAAATTGGCCGAGGAATGCAGGCAGGGGTATTGACCCTCTTTAA
CTGCCGCGTGCCGATCCTGA

Product: HemK protein

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKRFDAAPPHDPADIASLLAWAAGRLAAAGLDHPRREARLLMAHVLRTDLAGLLARSAMDAAEHRSFVALVARRAAHEPM
AYITGRAGFWSLDLETAPATLIPRADSETLVEALLAQRPDRGAVRTILDLGTGTGCLLLAALSEYPDAWGLGVDIDPDAA
HLAARNARRTGLRDRCAMLAADWSTAIAGRFDVVFSNPPYIPRADLAGLMPDVRDHEPARALDGGTDGLDAYRLLTGALP
SLLAHGGIAIFEIGIGQERSMPDLARQAGLDIVGIRTDLGGIPRAVLMQQCRV

Sequences:

>Translated_293_residues
MKRFDAAPPHDPADIASLLAWAAGRLAAAGLDHPRREARLLMAHVLRTDLAGLLARSAMDAAEHRSFVALVARRAAHEPM
AYITGRAGFWSLDLETAPATLIPRADSETLVEALLAQRPDRGAVRTILDLGTGTGCLLLAALSEYPDAWGLGVDIDPDAA
HLAARNARRTGLRDRCAMLAADWSTAIAGRFDVVFSNPPYIPRADLAGLMPDVRDHEPARALDGGTDGLDAYRLLTGALP
SLLAHGGIAIFEIGIGQERSMPDLARQAGLDIVGIRTDLGGIPRAVLMQQCRV
>Mature_293_residues
MKRFDAAPPHDPADIASLLAWAAGRLAAAGLDHPRREARLLMAHVLRTDLAGLLARSAMDAAEHRSFVALVARRAAHEPM
AYITGRAGFWSLDLETAPATLIPRADSETLVEALLAQRPDRGAVRTILDLGTGTGCLLLAALSEYPDAWGLGVDIDPDAA
HLAARNARRTGLRDRCAMLAADWSTAIAGRFDVVFSNPPYIPRADLAGLMPDVRDHEPARALDGGTDGLDAYRLLTGALP
SLLAHGGIAIFEIGIGQERSMPDLARQAGLDIVGIRTDLGGIPRAVLMQQCRV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=308, Percent_Identity=29.5454545454545, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1787463, Length=260, Percent_Identity=38.0769230769231, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082085, Length=267, Percent_Identity=30.7116104868914, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24582226, Length=255, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31205; Mature: 31205

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFDAAPPHDPADIASLLAWAAGRLAAAGLDHPRREARLLMAHVLRTDLAGLLARSAMD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEHRSFVALVARRAAHEPMAYITGRAGFWSLDLETAPATLIPRADSETLVEALLAQRPD
HHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCC
RGAVRTILDLGTGTGCLLLAALSEYPDAWGLGVDIDPDAAHLAARNARRTGLRDRCAMLA
CHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
ADWSTAIAGRFDVVFSNPPYIPRADLAGLMPDVRDHEPARALDGGTDGLDAYRLLTGALP
HHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHH
SLLAHGGIAIFEIGIGQERSMPDLARQAGLDIVGIRTDLGGIPRAVLMQQCRV
HHHHHCCEEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRFDAAPPHDPADIASLLAWAAGRLAAAGLDHPRREARLLMAHVLRTDLAGLLARSAMD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEHRSFVALVARRAAHEPMAYITGRAGFWSLDLETAPATLIPRADSETLVEALLAQRPD
HHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCC
RGAVRTILDLGTGTGCLLLAALSEYPDAWGLGVDIDPDAAHLAARNARRTGLRDRCAMLA
CHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
ADWSTAIAGRFDVVFSNPPYIPRADLAGLMPDVRDHEPARALDGGTDGLDAYRLLTGALP
HHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHH
SLLAHGGIAIFEIGIGQERSMPDLARQAGLDIVGIRTDLGGIPRAVLMQQCRV
HHHHHCCEEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]