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The map label for this gene is prfA

Identifier: 162147699

GI number: 162147699

Start: 1949964

End: 1951022

Strand: Direct

Name: prfA

Synonym: GDI_1915

Alternate gene names: 162147699

Gene position: 1949964-1951022 (Clockwise)

Preceding gene: 162147698

Following gene: 162147701

Centisome position: 49.44

GC content: 67.71

Gene sequence:

>1059_bases
ATGGGGCTCGACGACAGGCTGGACCGGATCGTCGCCCGGTCCGAGGAATTGCAGGCCGCGCTGGCCGAAGGGCTGGTCGG
GGAGGCGTTCGCCAAGGCCTCGCGCGAATATGCCGAGCTTGAGCCGATCGTCGACCGCATCGGCGAACTGCGCCTGGCGG
AGCAGGAAGAGCGCCAGTCGCAGGCGCTGCTGGCCGATCCGGAAATGCGCGAACTGGCCGAGGCCGAACTGGAAGATCTG
CGCGCGCGCATTCCCGTCCTGCGCCAGGATATCCGCATCGCCATGCTGCCGCGCGACGAGGCGGACGAGCGCAGCGCCAT
CCTGGAAATCCGTCCCGCCGCCGGCGGGGACGAGGCCGCCTTGTTCGCCGCCGAGCTGTTCGACGCCTATCGCCGCTATG
CCGCCCTGCGCGGCTGGCGGTTCGAGATCATGGAATTCGACGAATCCGAACTGGGCGGCCTGCGCGAGGGCATTGCCAAC
ATCACCGGGCGCGGCGTCTTCGCGCGCCTGAAATACGAATCCGGCGTGCATCGGGTCCAGCGCGTGCCCGCGACCGAAAG
CCAGGGGCGCATCCATACCTCGACGGTCACCGTGGCGGTGCTGCCCGAAGCCGGCGACGTGGACGTGCAGATCAATGACG
GCGACCTGCGCATCGACGTCTATCGGGCATCCGGCGCGGGCGGCCAGCATGTGAACAAGACCGAGAGCGCCGTGCGCATT
ACCCACCTGCCCACCGGCCTGGTGGTGGCGATGCAGGAGGAAAAGAGCCAGCACAAGAACCGTGCGAAGGCGATGAAGAT
CCTGATGGCGCGCCTGTACGAGCGCGAACGCGCGGCGGCCCATGCCACCCGCGCCGCCGACCGCAAGTCGCAGGTCGGCA
CCGGGGACCGCTCGGAACGGATACGCACCTATAATTTCCCGCAGGGCCGCGTGACCGATCACCGGATCAACCTGACCGCC
TACAAGATCGATCGCGTCATGATGGGCGAATTCGACGAATTCGTGGATGCGCTGACCCAGGACGAGCAGGCCAGCCTTCT
GGCGGCGGAGGGGCTGTAG

Upstream 100 bases:

>100_bases
CGCGGCGTGGCCCAGGTCAAGGCGCTGGATGACGGGTCGCAGGCCGAACTGGCCCTGGACGCCGTCACGCCCTATCTGGC
CGGGCTGGCCGGATAGACCG

Downstream 100 bases:

>100_bases
TTTCAGCAGCCGCTTACGAGGTCGGCACCGTGCAGAGGCTGGACCGGCCCGGATGATGCTCCACCACGGTAGGGTCCTCG
GGGTCGGCCAGATAGAATTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MGLDDRLDRIVARSEELQAALAEGLVGEAFAKASREYAELEPIVDRIGELRLAEQEERQSQALLADPEMRELAEAELEDL
RARIPVLRQDIRIAMLPRDEADERSAILEIRPAAGGDEAALFAAELFDAYRRYAALRGWRFEIMEFDESELGGLREGIAN
ITGRGVFARLKYESGVHRVQRVPATESQGRIHTSTVTVAVLPEAGDVDVQINDGDLRIDVYRASGAGGQHVNKTESAVRI
THLPTGLVVAMQEEKSQHKNRAKAMKILMARLYERERAAAHATRAADRKSQVGTGDRSERIRTYNFPQGRVTDHRINLTA
YKIDRVMMGEFDEFVDALTQDEQASLLAAEGL

Sequences:

>Translated_352_residues
MGLDDRLDRIVARSEELQAALAEGLVGEAFAKASREYAELEPIVDRIGELRLAEQEERQSQALLADPEMRELAEAELEDL
RARIPVLRQDIRIAMLPRDEADERSAILEIRPAAGGDEAALFAAELFDAYRRYAALRGWRFEIMEFDESELGGLREGIAN
ITGRGVFARLKYESGVHRVQRVPATESQGRIHTSTVTVAVLPEAGDVDVQINDGDLRIDVYRASGAGGQHVNKTESAVRI
THLPTGLVVAMQEEKSQHKNRAKAMKILMARLYERERAAAHATRAADRKSQVGTGDRSERIRTYNFPQGRVTDHRINLTA
YKIDRVMMGEFDEFVDALTQDEQASLLAAEGL
>Mature_351_residues
GLDDRLDRIVARSEELQAALAEGLVGEAFAKASREYAELEPIVDRIGELRLAEQEERQSQALLADPEMRELAEAELEDLR
ARIPVLRQDIRIAMLPRDEADERSAILEIRPAAGGDEAALFAAELFDAYRRYAALRGWRFEIMEFDESELGGLREGIANI
TGRGVFARLKYESGVHRVQRVPATESQGRIHTSTVTVAVLPEAGDVDVQINDGDLRIDVYRASGAGGQHVNKTESAVRIT
HLPTGLVVAMQEEKSQHKNRAKAMKILMARLYERERAAAHATRAADRKSQVGTGDRSERIRTYNFPQGRVTDHRINLTAY
KIDRVMMGEFDEFVDALTQDEQASLLAAEGL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=338, Percent_Identity=46.4497041420118, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI34577120, Length=359, Percent_Identity=38.1615598885794, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=241, Percent_Identity=46.4730290456432, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1787462, Length=348, Percent_Identity=52.8735632183908, Blast_Score=350, Evalue=6e-98,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=37.9821958456973, Blast_Score=194, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=304, Percent_Identity=38.4868421052632, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=42.9022082018927, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19921226, Length=321, Percent_Identity=42.6791277258567, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_GLUDA (A9HJ50)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602160.1
- RefSeq:   YP_002274552.1
- ProteinModelPortal:   A9HJ50
- SMR:   A9HJ50
- GeneID:   5790169
- GeneID:   6973529
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0137
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39264; Mature: 39133

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLDDRLDRIVARSEELQAALAEGLVGEAFAKASREYAELEPIVDRIGELRLAEQEERQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALLADPEMRELAEAELEDLRARIPVLRQDIRIAMLPRDEADERSAILEIRPAAGGDEAA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCCCCCHHH
LFAAELFDAYRRYAALRGWRFEIMEFDESELGGLREGIANITGRGVFARLKYESGVHRVQ
HHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
RVPATESQGRIHTSTVTVAVLPEAGDVDVQINDGDLRIDVYRASGAGGQHVNKTESAVRI
HCCCCCCCCEEEEEEEEEEEECCCCCEEEEEECCEEEEEEEEECCCCCCCCCCCCCEEEE
THLPTGLVVAMQEEKSQHKNRAKAMKILMARLYERERAAAHATRAADRKSQVGTGDRSER
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IRTYNFPQGRVTDHRINLTAYKIDRVMMGEFDEFVDALTQDEQASLLAAEGL
CEEECCCCCCCCCEEEEEEEEEEHHHHHCCHHHHHHHHCCCHHHHHHHCCCC
>Mature Secondary Structure 
GLDDRLDRIVARSEELQAALAEGLVGEAFAKASREYAELEPIVDRIGELRLAEQEERQS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALLADPEMRELAEAELEDLRARIPVLRQDIRIAMLPRDEADERSAILEIRPAAGGDEAA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCCCCCHHH
LFAAELFDAYRRYAALRGWRFEIMEFDESELGGLREGIANITGRGVFARLKYESGVHRVQ
HHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
RVPATESQGRIHTSTVTVAVLPEAGDVDVQINDGDLRIDVYRASGAGGQHVNKTESAVRI
HCCCCCCCCEEEEEEEEEEEECCCCCEEEEEECCEEEEEEEEECCCCCCCCCCCCCEEEE
THLPTGLVVAMQEEKSQHKNRAKAMKILMARLYERERAAAHATRAADRKSQVGTGDRSER
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IRTYNFPQGRVTDHRINLTAYKIDRVMMGEFDEFVDALTQDEQASLLAAEGL
CEEECCCCCCCCCEEEEEEEEEEHHHHHCCHHHHHHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA