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The map label for this gene is aarA [H]

Identifier: 162147614

GI number: 162147614

Start: 1862411

End: 1863712

Strand: Reverse

Name: aarA [H]

Synonym: GDI_1830

Alternate gene names: 162147614

Gene position: 1863712-1862411 (Counterclockwise)

Preceding gene: 162147615

Following gene: 162147612

Centisome position: 47.25

GC content: 63.52

Gene sequence:

>1302_bases
TTGATGACCGAGCCAGGAAAAGTGGCCACCGTCTCGCTAGAAGGCAAAACCGCCAGTCTGCCGGTCCTTTCCGGGACGCT
GGGCCCGGACGTTCTGGACGTCCGCAGGATCGCCGCCGACATGGGCGTCTTCACCTTCGACCCGGGCTATGGCGAAACGG
CGTCGTGCGAAAGCAAGATCACGTTCATCGACGGCGGCAAGGGCGTCCTGCTGCATCGTGGCTACCCGATCGCGCAGCTT
GCCGAGCAGGGCACGTTCCTCGAGGTCGCGTACCTGCTGCTGAACGGCGAACTGCCGACGACGGCGCAGCACGAGGCATT
CGTCTCGACCATCAAGTCGCACACGCTGCTGCATGAACAGATCCGCAATTTCTTCAACGGGTTCCGCCGCGACGCGCATC
CGATGGCGATCCTGTGCGGCACCGTCGGCGCGCTGTCGGCCTTCTATCACGAAGGCACGGACATCTCGAACGAGGCCAGC
CGCTCGCTGTCGGCGATGCGCCTGATCGCCAAGCTGCCGACGATCGCGGCCTGGGCGTACAAATACACGCAGGGCGAGCC
GTTCGTGTATCCGCGCAACGACCTGAATTACGCGGAAAACTTCCTGTCGATGATGTTCGCGCATCCGTCGGAGCCCTACG
TCATCAACCCGGTGCTGGCGCGGGCGATGGATCGCATCCTGATCCTGCATGCCGACCACGAACAGAATGCCTCGACCTCG
ACCGTGCGCCTGGCGGGCTCGACCGGCGCCAATCCGTTCGCGTGCATCGCCGCCGGCATCGCGGCGCTGTGGGGACCCGC
CCATGGCGGCGCGAACGAGGCCGTGCTGAAAATGCTGGCCGAAATCGGCAACAAGGACAACATCCCCGACTTCATCGCCA
AGGTGAAGGACAAGAACAGCGGCGTGCGCCTGATGGGCTTCGGCCATCGCGTGTACAAGAATTTCGACCCGCGCGCGAAG
ATCATGCAGGCCACCTGCCATGAGGTGCTGGGCGAACTGGGGATCAAGGACGATCCGCTGCTGGACCTGGCGGTCGAACT
GGAAAAGATCGCGATCAACGACGAATATTTCGTCAAGCGCAGCCTGTATCCCAACGTCGATTTCTATTCGGGCATCATCC
TGAAGGCGATGGGCATTCCCACCAGCATGTTCACGGTCCTGTTCGCCGTCGCGCGCACGGTGGGCTGGATCAGCCAATGG
AAGGAAATGATCGAGGAACCGGGCCAGCGCATCGGCCGTCCGCGCCAGCTCTATACCGGCGCCCCGCAGCGCGACTTCAC
GCCGTTCGCGCAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CGATGGGCCACGCCATGAACTGTCACGCAGAGGACAGCGGCCCGCAGGTTGGGGATGAGATCGGCCGCATCGCAGGGCGG
CGAACGACCAAGAGGAGAGT

Downstream 100 bases:

>100_bases
CCGGACCGGCAGGCCCGGGCGGACACGCCCGGGCAGCCCCGTGCCGCCCGCCGGGCATGAAAAAGCCGGCCATCCCGCGG
GGGTGGCCGGCTTTTTCATG

Product: citrate synthase

Products: NA

Alternate protein names: Acetic acid resistance protein [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MMTEPGKVATVSLEGKTASLPVLSGTLGPDVLDVRRIAADMGVFTFDPGYGETASCESKITFIDGGKGVLLHRGYPIAQL
AEQGTFLEVAYLLLNGELPTTAQHEAFVSTIKSHTLLHEQIRNFFNGFRRDAHPMAILCGTVGALSAFYHEGTDISNEAS
RSLSAMRLIAKLPTIAAWAYKYTQGEPFVYPRNDLNYAENFLSMMFAHPSEPYVINPVLARAMDRILILHADHEQNASTS
TVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLKMLAEIGNKDNIPDFIAKVKDKNSGVRLMGFGHRVYKNFDPRAK
IMQATCHEVLGELGIKDDPLLDLAVELEKIAINDEYFVKRSLYPNVDFYSGIILKAMGIPTSMFTVLFAVARTVGWISQW
KEMIEEPGQRIGRPRQLYTGAPQRDFTPFAQRG

Sequences:

>Translated_433_residues
MMTEPGKVATVSLEGKTASLPVLSGTLGPDVLDVRRIAADMGVFTFDPGYGETASCESKITFIDGGKGVLLHRGYPIAQL
AEQGTFLEVAYLLLNGELPTTAQHEAFVSTIKSHTLLHEQIRNFFNGFRRDAHPMAILCGTVGALSAFYHEGTDISNEAS
RSLSAMRLIAKLPTIAAWAYKYTQGEPFVYPRNDLNYAENFLSMMFAHPSEPYVINPVLARAMDRILILHADHEQNASTS
TVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLKMLAEIGNKDNIPDFIAKVKDKNSGVRLMGFGHRVYKNFDPRAK
IMQATCHEVLGELGIKDDPLLDLAVELEKIAINDEYFVKRSLYPNVDFYSGIILKAMGIPTSMFTVLFAVARTVGWISQW
KEMIEEPGQRIGRPRQLYTGAPQRDFTPFAQRG
>Mature_433_residues
MMTEPGKVATVSLEGKTASLPVLSGTLGPDVLDVRRIAADMGVFTFDPGYGETASCESKITFIDGGKGVLLHRGYPIAQL
AEQGTFLEVAYLLLNGELPTTAQHEAFVSTIKSHTLLHEQIRNFFNGFRRDAHPMAILCGTVGALSAFYHEGTDISNEAS
RSLSAMRLIAKLPTIAAWAYKYTQGEPFVYPRNDLNYAENFLSMMFAHPSEPYVINPVLARAMDRILILHADHEQNASTS
TVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLKMLAEIGNKDNIPDFIAKVKDKNSGVRLMGFGHRVYKNFDPRAK
IMQATCHEVLGELGIKDDPLLDLAVELEKIAINDEYFVKRSLYPNVDFYSGIILKAMGIPTSMFTVLFAVARTVGWISQW
KEMIEEPGQRIGRPRQLYTGAPQRDFTPFAQRG

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=352, Percent_Identity=28.6931818181818, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=65.8823529411765, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1786527, Length=385, Percent_Identity=30.3896103896104, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17555174, Length=442, Percent_Identity=29.185520361991, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319850, Length=374, Percent_Identity=31.0160427807487, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324328, Length=355, Percent_Identity=29.0140845070423, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325257, Length=360, Percent_Identity=27.5, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640124, Length=420, Percent_Identity=29.0476190476191, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24640126, Length=420, Percent_Identity=29.0476190476191, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356863, Length=364, Percent_Identity=28.2967032967033, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47458; Mature: 47458

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTEPGKVATVSLEGKTASLPVLSGTLGPDVLDVRRIAADMGVFTFDPGYGETASCESKI
CCCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCEE
TFIDGGKGVLLHRGYPIAQLAEQGTFLEVAYLLLNGELPTTAQHEAFVSTIKSHTLLHEQ
EEEECCCEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IRNFFNGFRRDAHPMAILCGTVGALSAFYHEGTDISNEASRSLSAMRLIAKLPTIAAWAY
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHE
KYTQGEPFVYPRNDLNYAENFLSMMFAHPSEPYVINPVLARAMDRILILHADHEQNASTS
EECCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEECHHHHHHHCCEEEEECCCCCCCCCC
TVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLKMLAEIGNKDNIPDFIAKVKDKNS
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GVRLMGFGHRVYKNFDPRAKIMQATCHEVLGELGIKDDPLLDLAVELEKIAINDEYFVKR
CEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCHHHHHH
SLYPNVDFYSGIILKAMGIPTSMFTVLFAVARTVGWISQWKEMIEEPGQRIGRPRQLYTG
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
APQRDFTPFAQRG
CCCCCCCCHHCCC
>Mature Secondary Structure
MMTEPGKVATVSLEGKTASLPVLSGTLGPDVLDVRRIAADMGVFTFDPGYGETASCESKI
CCCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCEE
TFIDGGKGVLLHRGYPIAQLAEQGTFLEVAYLLLNGELPTTAQHEAFVSTIKSHTLLHEQ
EEEECCCEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IRNFFNGFRRDAHPMAILCGTVGALSAFYHEGTDISNEASRSLSAMRLIAKLPTIAAWAY
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHE
KYTQGEPFVYPRNDLNYAENFLSMMFAHPSEPYVINPVLARAMDRILILHADHEQNASTS
EECCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEECHHHHHHHCCEEEEECCCCCCCCCC
TVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLKMLAEIGNKDNIPDFIAKVKDKNS
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GVRLMGFGHRVYKNFDPRAKIMQATCHEVLGELGIKDDPLLDLAVELEKIAINDEYFVKR
CEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCHHHHHH
SLYPNVDFYSGIILKAMGIPTSMFTVLFAVARTVGWISQWKEMIEEPGQRIGRPRQLYTG
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
APQRDFTPFAQRG
CCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156811 [H]