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The map label for this gene is pyrE [H]

Identifier: 162147612

GI number: 162147612

Start: 1861037

End: 1861798

Strand: Reverse

Name: pyrE [H]

Synonym: GDI_1828

Alternate gene names: 162147612

Gene position: 1861798-1861037 (Counterclockwise)

Preceding gene: 162147614

Following gene: 162147611

Centisome position: 47.2

GC content: 66.4

Gene sequence:

>762_bases
ATGAACGATTCGACAGGATATGGCACGGGCGCCTCGTCGGGCTCCACGGCCTGGGACCGCGACGCCGCGCTGACGACGGC
GAAGCTGCTGCTGGAAATCAAGGCGGTCAATTTCCGGCCCGAGGAACCCTACACCCTGACGTCGGGCTGGAAGTCGCCGG
TCTATATCGACTGCCGGCGCATCATCTTCTTCCCCCGCGCCCGCGCCAAGATCGTGGAACTGGGGGTGGAAAAGATCGGC
CGCCATATCGGTTATGAGAGCATCGACGCCGTGGTGGGCGGGGAAACGGCGGGCATCCCCTTCGCCGCCTGGATCGCGGA
CCGGATGATGGCCCCCATGGCTTATGTCCGCAAGAAGCCCAAGGGGTTCGGTCGCAACGCGCAGATCGAGGGCGACGTGC
CCGAGGGCATGCGCACCCTGCTGGTCGAGGACCTGACGACCGACGGATCGTCCAAGATCCGCTTCGCCCGCGCCCTGCGC
GACGCCGGGGCGATCGTCGACCACACCTTCGTCGTCTTCTTCTACGGCGTGTTCCCCGGTTCGCTGCGCACGCTGGCGGA
CATGAACATCTCGCTGCACGCGCTGTGCACCTGGTGGGACGTGCTGGAAGCCTGCGCGGGCCAGCCCTATTTCTCGGAAA
AGGCGGCATCCGAGGTCCGGCGCTTCCTGGAAGACCCCTGCGAATGGTCGGGCCGCCATGGCGGCGTTTCCAGCCAGGAG
GAAGCCCTGGCGTTCAAGGGCGCGAACCAGACCCAGGGCTGA

Upstream 100 bases:

>100_bases
CATACAGCGTCCCAATTGGCGGACGATGGATTTTCCCGCCCGATCCGGCTAAGGCTCATGCACCTCAATGCAATGATACC
AGACCGGAGAGATACGCGGC

Downstream 100 bases:

>100_bases
TGCACGACCAGGACTGACTTTTCATCATGGCGCGGCGAATCCTGGCGTTCGATTCCGGCATCGGCGGGTTCGGAATCGTC
CGGGCCCTTCGCGCGCGGCT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNDSTGYGTGASSGSTAWDRDAALTTAKLLLEIKAVNFRPEEPYTLTSGWKSPVYIDCRRIIFFPRARAKIVELGVEKIG
RHIGYESIDAVVGGETAGIPFAAWIADRMMAPMAYVRKKPKGFGRNAQIEGDVPEGMRTLLVEDLTTDGSSKIRFARALR
DAGAIVDHTFVVFFYGVFPGSLRTLADMNISLHALCTWWDVLEACAGQPYFSEKAASEVRRFLEDPCEWSGRHGGVSSQE
EALAFKGANQTQG

Sequences:

>Translated_253_residues
MNDSTGYGTGASSGSTAWDRDAALTTAKLLLEIKAVNFRPEEPYTLTSGWKSPVYIDCRRIIFFPRARAKIVELGVEKIG
RHIGYESIDAVVGGETAGIPFAAWIADRMMAPMAYVRKKPKGFGRNAQIEGDVPEGMRTLLVEDLTTDGSSKIRFARALR
DAGAIVDHTFVVFFYGVFPGSLRTLADMNISLHALCTWWDVLEACAGQPYFSEKAASEVRRFLEDPCEWSGRHGGVSSQE
EALAFKGANQTQG
>Mature_253_residues
MNDSTGYGTGASSGSTAWDRDAALTTAKLLLEIKAVNFRPEEPYTLTSGWKSPVYIDCRRIIFFPRARAKIVELGVEKIG
RHIGYESIDAVVGGETAGIPFAAWIADRMMAPMAYVRKKPKGFGRNAQIEGDVPEGMRTLLVEDLTTDGSSKIRFARALR
DAGAIVDHTFVVFFYGVFPGSLRTLADMNISLHALCTWWDVLEACAGQPYFSEKAASEVRRFLEDPCEWSGRHGGVSSQE
EALAFKGANQTQG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=192, Percent_Identity=28.6458333333333, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17508631, Length=190, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17933654, Length=150, Percent_Identity=31.3333333333333, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 27738; Mature: 27738

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSTGYGTGASSGSTAWDRDAALTTAKLLLEIKAVNFRPEEPYTLTSGWKSPVYIDCRR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEE
IIFFPRARAKIVELGVEKIGRHIGYESIDAVVGGETAGIPFAAWIADRMMAPMAYVRKKP
EEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
KGFGRNAQIEGDVPEGMRTLLVEDLTTDGSSKIRFARALRDAGAIVDHTFVVFFYGVFPG
CCCCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
SLRTLADMNISLHALCTWWDVLEACAGQPYFSEKAASEVRRFLEDPCEWSGRHGGVSSQE
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
EALAFKGANQTQG
HHHEECCCCCCCC
>Mature Secondary Structure
MNDSTGYGTGASSGSTAWDRDAALTTAKLLLEIKAVNFRPEEPYTLTSGWKSPVYIDCRR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEE
IIFFPRARAKIVELGVEKIGRHIGYESIDAVVGGETAGIPFAAWIADRMMAPMAYVRKKP
EEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
KGFGRNAQIEGDVPEGMRTLLVEDLTTDGSSKIRFARALRDAGAIVDHTFVVFFYGVFPG
CCCCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
SLRTLADMNISLHALCTWWDVLEACAGQPYFSEKAASEVRRFLEDPCEWSGRHGGVSSQE
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
EALAFKGANQTQG
HHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA