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The map label for this gene is nrdF [H]

Identifier: 162147608

GI number: 162147608

Start: 1857139

End: 1858200

Strand: Direct

Name: nrdF [H]

Synonym: GDI_1824

Alternate gene names: 162147608

Gene position: 1857139-1858200 (Clockwise)

Preceding gene: 162147607

Following gene: 162147610

Centisome position: 47.09

GC content: 61.86

Gene sequence:

>1062_bases
ATGACCGACATCACCAACGCGACCGGCACGGCACCGGAGAGCGCGCCCCAGCCGACAGGGGTCGAGGCGCGCCTCGACCT
GCTGACGTCCAATCCGATCTACAAGCCGTTCCGCTACCCCTGGGCGTACGATGCGTGGCTGACGCAGCAGCGCGTGCACT
GGCTGCCCGAGGAAGTGCCGCTGGCCGATGACGTCAAGGACTGGCACCGCACCCTGACCGAGAGCGAGCAGCATCTGGTC
ACGCAGATCTTCCGCTTCTTCACGCAGTCGGACGTGGAGGTGAACAACTGCTACATGAAGCACTACAGCCGGGTATTCAA
ACCGACCGAAGTGCTGATGATGCTGTCCGCCTTCTCGAACATCGAGACGATCCATATCGCCGCCTACAGTCATCTGCTCG
ACACCATCGGCATGCCCGAGATGGAATACTCGGCCTTCCTCAAATACAAGGAGATGAAGGACAAGTATGACTACATGCAG
GGCTTCTCGGTGGACAGCAAGCCCGAGATCGCCCGCACGCTGGCGGCCTTCGGCGCGTTCACCGAGGGGCTGCAGCTCTT
CGCGTCCTTCGCCATCCTGCTGAACTTCCCGCGCTTCAACAAGCTGAAGGGCATGGGGCAGATCGTGTCGTGGTCGGTGC
GCGACGAAACGCTGCACTGCCTGTCCATGGCCCGGCTGTTCCGCGTGTTCATCCAGGAAAATCCCGAAATCTGGACCGAC
GCCCTGCGCGCGGACATCGTCCAGATCTGCCGCGACATCGTCGAGCACGAGGACGCCTTCATCGATCTGTGCTTCGAGAT
GGGGCCGGTCGAAGGGCTGGATGGCGACCTGGTCAAGACCTATATCCGCTTCATCGCGGACCGGCGGCTGAACCTGCTGG
GGCTGGACGGGATCTATGACGTGCCCAGCAATCCGCTGCCGTGGGTGGACGACATGCTGAACGCCGTCGAGCACACCAAT
TTCTTCGAAAACCGCTCCACGGAATACAGCCGCGCCTCGACCACGGGGTCGTGGGAAGAGGCGTTCGAGGAAAGCGTTTT
CCAGAACGGAAACGCGGGCTGA

Upstream 100 bases:

>100_bases
TCATGGCCGACGAACCTTCCGCTCCTGCTCCCGCTCCCGCCAGCCCGCCGGCATCTTCCAACAATTACGACGAATGCCTG
GCCTGCCAGTGAGAGACGAC

Downstream 100 bases:

>100_bases
CCGTCAGGCGATCGGCGCGCCGTCGCGCAGGACGGCTTCGGCCTGCGGGGTGAAGCTGCCGTCGTCGCGGTGCAGGGTCA
TGCCCGGCATCAGGACGGCC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTDITNATGTAPESAPQPTGVEARLDLLTSNPIYKPFRYPWAYDAWLTQQRVHWLPEEVPLADDVKDWHRTLTESEQHLV
TQIFRFFTQSDVEVNNCYMKHYSRVFKPTEVLMMLSAFSNIETIHIAAYSHLLDTIGMPEMEYSAFLKYKEMKDKYDYMQ
GFSVDSKPEIARTLAAFGAFTEGLQLFASFAILLNFPRFNKLKGMGQIVSWSVRDETLHCLSMARLFRVFIQENPEIWTD
ALRADIVQICRDIVEHEDAFIDLCFEMGPVEGLDGDLVKTYIRFIADRRLNLLGLDGIYDVPSNPLPWVDDMLNAVEHTN
FFENRSTEYSRASTTGSWEEAFEESVFQNGNAG

Sequences:

>Translated_353_residues
MTDITNATGTAPESAPQPTGVEARLDLLTSNPIYKPFRYPWAYDAWLTQQRVHWLPEEVPLADDVKDWHRTLTESEQHLV
TQIFRFFTQSDVEVNNCYMKHYSRVFKPTEVLMMLSAFSNIETIHIAAYSHLLDTIGMPEMEYSAFLKYKEMKDKYDYMQ
GFSVDSKPEIARTLAAFGAFTEGLQLFASFAILLNFPRFNKLKGMGQIVSWSVRDETLHCLSMARLFRVFIQENPEIWTD
ALRADIVQICRDIVEHEDAFIDLCFEMGPVEGLDGDLVKTYIRFIADRRLNLLGLDGIYDVPSNPLPWVDDMLNAVEHTN
FFENRSTEYSRASTTGSWEEAFEESVFQNGNAG
>Mature_352_residues
TDITNATGTAPESAPQPTGVEARLDLLTSNPIYKPFRYPWAYDAWLTQQRVHWLPEEVPLADDVKDWHRTLTESEQHLVT
QIFRFFTQSDVEVNNCYMKHYSRVFKPTEVLMMLSAFSNIETIHIAAYSHLLDTIGMPEMEYSAFLKYKEMKDKYDYMQG
FSVDSKPEIARTLAAFGAFTEGLQLFASFAILLNFPRFNKLKGMGQIVSWSVRDETLHCLSMARLFRVFIQENPEIWTDA
LRADIVQICRDIVEHEDAFIDLCFEMGPVEGLDGDLVKTYIRFIADRRLNLLGLDGIYDVPSNPLPWVDDMLNAVEHTNF
FENRSTEYSRASTTGSWEEAFEESVFQNGNAG

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=300, Percent_Identity=28.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI289177074, Length=300, Percent_Identity=29, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4557845, Length=340, Percent_Identity=28.2352941176471, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260064013, Length=340, Percent_Identity=28.2352941176471, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI289177076, Length=279, Percent_Identity=28.3154121863799, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17551968, Length=288, Percent_Identity=30.2083333333333, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322434, Length=306, Percent_Identity=27.4509803921569, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18079279, Length=318, Percent_Identity=29.2452830188679, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40630; Mature: 40499

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDITNATGTAPESAPQPTGVEARLDLLTSNPIYKPFRYPWAYDAWLTQQRVHWLPEEVP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
LADDVKDWHRTLTESEQHLVTQIFRFFTQSDVEVNNCYMKHYSRVFKPTEVLMMLSAFSN
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCC
IETIHIAAYSHLLDTIGMPEMEYSAFLKYKEMKDKYDYMQGFSVDSKPEIARTLAAFGAF
CCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TEGLQLFASFAILLNFPRFNKLKGMGQIVSWSVRDETLHCLSMARLFRVFIQENPEIWTD
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHEECHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALRADIVQICRDIVEHEDAFIDLCFEMGPVEGLDGDLVKTYIRFIADRRLNLLGLDGIYD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCC
VPSNPLPWVDDMLNAVEHTNFFENRSTEYSRASTTGSWEEAFEESVFQNGNAG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDITNATGTAPESAPQPTGVEARLDLLTSNPIYKPFRYPWAYDAWLTQQRVHWLPEEVP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
LADDVKDWHRTLTESEQHLVTQIFRFFTQSDVEVNNCYMKHYSRVFKPTEVLMMLSAFSN
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCC
IETIHIAAYSHLLDTIGMPEMEYSAFLKYKEMKDKYDYMQGFSVDSKPEIARTLAAFGAF
CCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TEGLQLFASFAILLNFPRFNKLKGMGQIVSWSVRDETLHCLSMARLFRVFIQENPEIWTD
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHEECHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALRADIVQICRDIVEHEDAFIDLCFEMGPVEGLDGDLVKTYIRFIADRRLNLLGLDGIYD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCC
VPSNPLPWVDDMLNAVEHTNFFENRSTEYSRASTTGSWEEAFEESVFQNGNAG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]