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The map label for this gene is glpK [H]

Identifier: 162147537

GI number: 162147537

Start: 1777217

End: 1778716

Strand: Direct

Name: glpK [H]

Synonym: GDI_1753

Alternate gene names: 162147537

Gene position: 1777217-1778716 (Clockwise)

Preceding gene: 162147536

Following gene: 162147539

Centisome position: 45.06

GC content: 70.33

Gene sequence:

>1500_bases
ATGAACAAGAAGACGACCATCCTGGCGATCGATCAGGGCACGACGTCCACCCGCAGCATCATCTTCGATCGCGACGCGAC
GCCGCTGGCGACATCGCGGATCGAGTTCGCGCAGCATTATCCCGACCATGGCTGGGTCGAGCATGACGCGGAAGAGATCT
GGCGCGATGCGCTTTCGACCGCGCGCGCGGCGATCGAACAGGGCGGCGGGGTGGATGCGATCGCGGCCCTGGGCATCACC
AACCAGCGCGAGACCATCGTGGTCTGGGACCGCGCGACCGGAACCCCCATCCATCGCGCCATCGTCTGGCAGGACCGCCG
CACGGCGTCCATGTGCGCCCGCATGCGCCAGGACGGGTGCGAGGGGCTGGTGCGCGACCGGACCGGCCTGCTGCTGGACC
CCTATTTTTCCGCCAGCAAGCTGGCCTGGATGCTGGACAACGTCCCCGGCGCGCGCGCGTCCGCCGAAGCCGGGCGCCTG
GCCTTCGGCACCATCGACAGCTTCCTGCTGTGGCGCCTGACCGGCGGGCGGGTCCATGCCACCGATGTGACGAACGCGTC
GCGCACCCTGCTGTTCGACATCCATCGCCAGGTCTGGGACGACGACCTGCTGCGGTTGTTCGGCATTCCGGCGGCGCTGC
TGCCCGAGGTTCGGGGGAACAGCGAAATCTACGGCGAAACCGATCCCGCCCTGTTCGGCCGGCCGATTCCGGTGGCGGGC
ATGGCCGGGGACCAGCAGGCGGCGCTGGTGGGCCAGGCCTGCTTCGCCCCCGGAATGGCCAAGGCGACCTATGGCACCGG
CTGCTTCGTCCTGCTGAACACCGGCACGGCCCCGGTGCAGTCGGCCAACCGGATGCTGACCACGATCGGCTACCGGATCG
GCGACCGCACGACCTATGCGCTGGAAGGGGCGATCTTCGTCGCGGGGGCCGCGATCAAGTGGCTGCGCGACGGGCTGCAC
CTGATCACCCACGCGTCGCAGACCGACGACATGGCGACGCGTATTCCCCACAGTCACGGCGTCTACATGGTGCCGGGATT
CGTGGGCCTGGGCGCGCCGCATTGGGACCCCGAGGCCAGGGGGCTGATCTGCGGCCTGACGCTGGACGCGACGGCGGCGC
ATATCGCCCGCGCGGCGCTGGAATCGGTCGCCTACCAGACATACGATCTGATGGCGGCGATGGCGCAGGACGGCGGCGGG
GCGGTGACCACCCTGCGTGTCGATGGCGGCATGGCGGCCAATGACTGGTTCTGCCGGTTCCTGTCCGACGTGCTGCAGGC
CCGGGTGGAGCGGCCGCGCCACCTGGAAACCACGGCGACCGGCGCCGCCTTCCTGGCCGGGCTGGCCACGGGGGTGTGGA
CGGACCTGGATGACGTGGCCGCCCGCCGGGCGCGCGGCAGCCTGTTCCAGCCCACGATGGACCTGGCCCAGCGCGGTACC
ATGCTGGACGGCTGGCACCAGGCGGTCCGGCGCACCCTGACCGCCGACGCTTCCGCGTAG

Upstream 100 bases:

>100_bases
CGCCGGCAGCCGCCGGTCCTGGTCGAGACCCTGCCCGCATCCGATGCCGCGATCCGGCGGTCGTCGTGACGGTCATTCCA
AGTCGGGAGACAAGAAAATC

Downstream 100 bases:

>100_bases
ACGGTCGAGTTTTAGGGTGCGTGATCAGGTGGTTCCACCTCATCACGATCTGCTTTAAGGGGCGGTATCGGCCGTGTCGG
GTGCCGGACGCACGTCGAAC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNKKTTILAIDQGTTSTRSIIFDRDATPLATSRIEFAQHYPDHGWVEHDAEEIWRDALSTARAAIEQGGGVDAIAALGIT
NQRETIVVWDRATGTPIHRAIVWQDRRTASMCARMRQDGCEGLVRDRTGLLLDPYFSASKLAWMLDNVPGARASAEAGRL
AFGTIDSFLLWRLTGGRVHATDVTNASRTLLFDIHRQVWDDDLLRLFGIPAALLPEVRGNSEIYGETDPALFGRPIPVAG
MAGDQQAALVGQACFAPGMAKATYGTGCFVLLNTGTAPVQSANRMLTTIGYRIGDRTTYALEGAIFVAGAAIKWLRDGLH
LITHASQTDDMATRIPHSHGVYMVPGFVGLGAPHWDPEARGLICGLTLDATAAHIARAALESVAYQTYDLMAAMAQDGGG
AVTTLRVDGGMAANDWFCRFLSDVLQARVERPRHLETTATGAAFLAGLATGVWTDLDDVAARRARGSLFQPTMDLAQRGT
MLDGWHQAVRRTLTADASA

Sequences:

>Translated_499_residues
MNKKTTILAIDQGTTSTRSIIFDRDATPLATSRIEFAQHYPDHGWVEHDAEEIWRDALSTARAAIEQGGGVDAIAALGIT
NQRETIVVWDRATGTPIHRAIVWQDRRTASMCARMRQDGCEGLVRDRTGLLLDPYFSASKLAWMLDNVPGARASAEAGRL
AFGTIDSFLLWRLTGGRVHATDVTNASRTLLFDIHRQVWDDDLLRLFGIPAALLPEVRGNSEIYGETDPALFGRPIPVAG
MAGDQQAALVGQACFAPGMAKATYGTGCFVLLNTGTAPVQSANRMLTTIGYRIGDRTTYALEGAIFVAGAAIKWLRDGLH
LITHASQTDDMATRIPHSHGVYMVPGFVGLGAPHWDPEARGLICGLTLDATAAHIARAALESVAYQTYDLMAAMAQDGGG
AVTTLRVDGGMAANDWFCRFLSDVLQARVERPRHLETTATGAAFLAGLATGVWTDLDDVAARRARGSLFQPTMDLAQRGT
MLDGWHQAVRRTLTADASA
>Mature_499_residues
MNKKTTILAIDQGTTSTRSIIFDRDATPLATSRIEFAQHYPDHGWVEHDAEEIWRDALSTARAAIEQGGGVDAIAALGIT
NQRETIVVWDRATGTPIHRAIVWQDRRTASMCARMRQDGCEGLVRDRTGLLLDPYFSASKLAWMLDNVPGARASAEAGRL
AFGTIDSFLLWRLTGGRVHATDVTNASRTLLFDIHRQVWDDDLLRLFGIPAALLPEVRGNSEIYGETDPALFGRPIPVAG
MAGDQQAALVGQACFAPGMAKATYGTGCFVLLNTGTAPVQSANRMLTTIGYRIGDRTTYALEGAIFVAGAAIKWLRDGLH
LITHASQTDDMATRIPHSHGVYMVPGFVGLGAPHWDPEARGLICGLTLDATAAHIARAALESVAYQTYDLMAAMAQDGGG
AVTTLRVDGGMAANDWFCRFLSDVLQARVERPRHLETTATGAAFLAGLATGVWTDLDDVAARRARGSLFQPTMDLAQRGT
MLDGWHQAVRRTLTADASA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=44.4444444444444, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=44.4444444444444, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=43.921568627451, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI41393575, Length=500, Percent_Identity=43.4, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=34.1846758349705, Blast_Score=312, Evalue=5e-85,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=51.5151515151515, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789987, Length=440, Percent_Identity=26.1363636363636, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790005, Length=480, Percent_Identity=24.5833333333333, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=496, Percent_Identity=40.7258064516129, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71986140, Length=516, Percent_Identity=25.3875968992248, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71986147, Length=299, Percent_Identity=28.7625418060201, Blast_Score=131, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=495, Percent_Identity=34.9494949494949, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=41.5841584158416, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24654573, Length=448, Percent_Identity=42.6339285714286, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655555, Length=499, Percent_Identity=39.2785571142285, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24655560, Length=499, Percent_Identity=39.2785571142285, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24655570, Length=499, Percent_Identity=39.2785571142285, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24655574, Length=499, Percent_Identity=39.2785571142285, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24655565, Length=499, Percent_Identity=39.2785571142285, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=33.1428571428571, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=33.6048879837067, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24656367, Length=505, Percent_Identity=29.1089108910891, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21357867, Length=521, Percent_Identity=28.4069097888676, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24656372, Length=345, Percent_Identity=31.8840579710145, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45552909, Length=259, Percent_Identity=30.8880308880309, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 53843; Mature: 53843

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTTILAIDQGTTSTRSIIFDRDATPLATSRIEFAQHYPDHGWVEHDAEEIWRDALST
CCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ARAAIEQGGGVDAIAALGITNQRETIVVWDRATGTPIHRAIVWQDRRTASMCARMRQDGC
HHHHHHCCCCCCHHHEECCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHH
EGLVRDRTGLLLDPYFSASKLAWMLDNVPGARASAEAGRLAFGTIDSFLLWRLTGGRVHA
HHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHEEECCCEEEE
TDVTNASRTLLFDIHRQVWDDDLLRLFGIPAALLPEVRGNSEIYGETDPALFGRPIPVAG
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCC
MAGDQQAALVGQACFAPGMAKATYGTGCFVLLNTGTAPVQSANRMLTTIGYRIGDRTTYA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
LEGAIFVAGAAIKWLRDGLHLITHASQTDDMATRIPHSHGVYMVPGFVGLGAPHWDPEAR
ECCEEEEHHHHHHHHHCCHHHHHCCCCCCHHHHHCCCCCCCEECCCHHCCCCCCCCCCCC
GLICGLTLDATAAHIARAALESVAYQTYDLMAAMAQDGGGAVTTLRVDGGMAANDWFCRF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHH
LSDVLQARVERPRHLETTATGAAFLAGLATGVWTDLDDVAARRARGSLFQPTMDLAQRGT
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCCC
MLDGWHQAVRRTLTADASA
CCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKKTTILAIDQGTTSTRSIIFDRDATPLATSRIEFAQHYPDHGWVEHDAEEIWRDALST
CCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ARAAIEQGGGVDAIAALGITNQRETIVVWDRATGTPIHRAIVWQDRRTASMCARMRQDGC
HHHHHHCCCCCCHHHEECCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHH
EGLVRDRTGLLLDPYFSASKLAWMLDNVPGARASAEAGRLAFGTIDSFLLWRLTGGRVHA
HHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHEEECCCEEEE
TDVTNASRTLLFDIHRQVWDDDLLRLFGIPAALLPEVRGNSEIYGETDPALFGRPIPVAG
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCC
MAGDQQAALVGQACFAPGMAKATYGTGCFVLLNTGTAPVQSANRMLTTIGYRIGDRTTYA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
LEGAIFVAGAAIKWLRDGLHLITHASQTDDMATRIPHSHGVYMVPGFVGLGAPHWDPEAR
ECCEEEEHHHHHHHHHCCHHHHHCCCCCCHHHHHCCCCCCCEECCCHHCCCCCCCCCCCC
GLICGLTLDATAAHIARAALESVAYQTYDLMAAMAQDGGGAVTTLRVDGGMAANDWFCRF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHH
LSDVLQARVERPRHLETTATGAAFLAGLATGVWTDLDDVAARRARGSLFQPTMDLAQRGT
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCCC
MLDGWHQAVRRTLTADASA
CCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA