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The map label for this gene is gyrB [H]

Identifier: 162147271

GI number: 162147271

Start: 1484068

End: 1486104

Strand: Reverse

Name: gyrB [H]

Synonym: GDI_1476

Alternate gene names: 162147271

Gene position: 1486104-1484068 (Counterclockwise)

Preceding gene: 162147272

Following gene: 162147270

Centisome position: 37.68

GC content: 69.46

Gene sequence:

>2037_bases
ATGAACGATGATCTCTTTTCCGCGGCGCGACCGAAGCGGGAGCCCCGGGGCCGCGGGGCCGCGTCCGCCCCGCCGGCCCC
GGTGCGGAACGATGCCGGCGGGGAGTACGACGCCAGCGCCATCGAGGTGCTGGAGGGGCTGGAACCCGTCCGGCGCCGGC
CGGGCATGTATATCGGTGGCACCGACGAGGGCGCATACCACCACCTGGCGTCCGAGATCCTGGACAATGCGATGGACGAG
GCCGTGGCCGGCCACGCCACCACCATCGACGTCACGCTGGAAGCAGGGGACTGGCTGACGATCCGCGACAACGGGCGCGG
CATTCCGGTCGACCCGCATCCGAAATTCAAGGATCGCTCGGCGCTGGAGGTCATCCTGACCACGCTGCACGCGGGCGGCA
AGTTCTCGGGCAAGGCCTATGCCACCTCGGGCGGCCTGCACGGGGTGGGATCGTCGGTGGTCAACGCCCTGGCGGCACGG
ATGGAGGTCGAGGTCGCCCGCGACCGCGCCGTCTGGCGTCAGGTGTACGAGCGCGGCCATCCCGTCACGATGCTGGAGAA
GGTCGGCGCGACCCAGAACCGGCGCGGCACGCAGATCCGCTTCACCCCCGATCCGCAGATCTTCGGCCGGATCCATTTCT
CGCCCGCGCGGCTGTACCGGCTGTGCCGGTCCAAGGCGTACCTGTTCCGCGGCGTCACCATCCGCTGGTCGTGCGACCCG
GCGCTGGTCCGCGACGCGGACATCCCGGCCGAGGCGGTGCTGCACTTCCCCGGCGGCCTGGCCGACAGCCTGCGCGACGA
ACTGGGCAACGCCGCCCTGCTGACCGAGATGTGGTCCGGCGAGGCCGACCTGCCGGTGGGGCCCGACGGCGCCGATACCG
GCCGGATGGAATGGGCGGTGGCGTTCCTGGAATCGGGGTCGGCCAGCCTGTCCTCGTACTGCAACACCATCCCGACGCCG
CAGGGCGGCACGCACGAAGCCGGGTTTCGCGCCGCGCTGCTGAAGGGCTTCCGCGCCTGGGGCGAACAGCGGTCGAACAA
GCGCGCGGCGGCGGTCAACGCCGATGACGTGCTGGGCGCCATCGCCGCCAAGCTGTCGGTGTTCATCCGCGACCCGCAAT
TCCAGGGCCAGACCAAGGAAAAGCTGACCTCGTCCGAGGCCAGCCGCCTGATCGAGACCGCGCTGCGCGACCGGTTCGAG
CAATGGCTGGCCGGCCATCCGGCACAGGCCGACAACCTGCTGGCCTTCATCATCGAGCGGGCCGAGGAACGGCTGCGCCG
CAAGGACCTGAAAGACACGCCGCGCAAGAGCGCGACCCGGCGGCTGCGCCTGCCCGGCAAGCTGACCGACTGCACGCGCG
AGAACGCGGCCGAGACCGAGATCTTCCTGGTCGAGGGCGATTCGGCCGGCGGGTCGGCCAAGCAGGCCCGAAACCGCGAA
ACCCAGGCCGTGCTGCCGCTGCGCGGCAAGATCCTGAACGTCGCCAGCGCCTCGACCGAGAAGCTGCGCGCCAACCAGGA
ACTGCGCGACCTGGTCGAGGCCCTGGGCTGCGGCATCGGCGAACGGTTCGACGCGACCCGCCTGCGCTACGGCCGCATCG
TCATCATGACCGACGCCGACGTGGACGGCGCGCATATCGCGTCGCTGCTGATGACGTTCTTCTATCGCGAACTGCCCGAC
CTGATCCGCCAGGGGCATCTGTACCTGGCGCAGCCGCCGCTGTACCGGCTGACGCAGGGCGGCAAGACGATCTATGCGAT
GGACGACACCGACCGCGAGCGGAAGCTGAAGTCGAACTTCAAGCCGAATGCCAAGGTCGAGGTCTCGCGCTTCAAGGGCC
TGGGCGAGATGCCGCCGGGCGATCTGAAGCAGACGACGATGGACCCACGGCACCGCACGCTGCTGAAAGTCGTCACCCGT
CCCGAGGACCGCGCCCTGACCAGCGACCGGGTCGAACATCTGATGGGACGGCGGCCGGAACTGCGCTTCCAGTTCATTCA
GGAACACGCGCAGACCGTCGAAGACCTGGACGTTTGA

Upstream 100 bases:

>100_bases
GCCACAAGCCGTCCGCCGCCCACACGTCGGCGCGGAGCTGACGCCGCCCCGGACCGAGTCCGCTTGACAGCGGACCGGCC
GGCGGAACAAAGAACACTCC

Downstream 100 bases:

>100_bases
ATGTCTGTCCGGGCTGCGCCCCGGGCCGCAGGAGTTCCATGACCGCCAGTACATCCCAGGCCGCTCTTGCACCCGGCGCG
CCCGCCGACGGGCAATCGGA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MNDDLFSAARPKREPRGRGAASAPPAPVRNDAGGEYDASAIEVLEGLEPVRRRPGMYIGGTDEGAYHHLASEILDNAMDE
AVAGHATTIDVTLEAGDWLTIRDNGRGIPVDPHPKFKDRSALEVILTTLHAGGKFSGKAYATSGGLHGVGSSVVNALAAR
MEVEVARDRAVWRQVYERGHPVTMLEKVGATQNRRGTQIRFTPDPQIFGRIHFSPARLYRLCRSKAYLFRGVTIRWSCDP
ALVRDADIPAEAVLHFPGGLADSLRDELGNAALLTEMWSGEADLPVGPDGADTGRMEWAVAFLESGSASLSSYCNTIPTP
QGGTHEAGFRAALLKGFRAWGEQRSNKRAAAVNADDVLGAIAAKLSVFIRDPQFQGQTKEKLTSSEASRLIETALRDRFE
QWLAGHPAQADNLLAFIIERAEERLRRKDLKDTPRKSATRRLRLPGKLTDCTRENAAETEIFLVEGDSAGGSAKQARNRE
TQAVLPLRGKILNVASASTEKLRANQELRDLVEALGCGIGERFDATRLRYGRIVIMTDADVDGAHIASLLMTFFYRELPD
LIRQGHLYLAQPPLYRLTQGGKTIYAMDDTDRERKLKSNFKPNAKVEVSRFKGLGEMPPGDLKQTTMDPRHRTLLKVVTR
PEDRALTSDRVEHLMGRRPELRFQFIQEHAQTVEDLDV

Sequences:

>Translated_678_residues
MNDDLFSAARPKREPRGRGAASAPPAPVRNDAGGEYDASAIEVLEGLEPVRRRPGMYIGGTDEGAYHHLASEILDNAMDE
AVAGHATTIDVTLEAGDWLTIRDNGRGIPVDPHPKFKDRSALEVILTTLHAGGKFSGKAYATSGGLHGVGSSVVNALAAR
MEVEVARDRAVWRQVYERGHPVTMLEKVGATQNRRGTQIRFTPDPQIFGRIHFSPARLYRLCRSKAYLFRGVTIRWSCDP
ALVRDADIPAEAVLHFPGGLADSLRDELGNAALLTEMWSGEADLPVGPDGADTGRMEWAVAFLESGSASLSSYCNTIPTP
QGGTHEAGFRAALLKGFRAWGEQRSNKRAAAVNADDVLGAIAAKLSVFIRDPQFQGQTKEKLTSSEASRLIETALRDRFE
QWLAGHPAQADNLLAFIIERAEERLRRKDLKDTPRKSATRRLRLPGKLTDCTRENAAETEIFLVEGDSAGGSAKQARNRE
TQAVLPLRGKILNVASASTEKLRANQELRDLVEALGCGIGERFDATRLRYGRIVIMTDADVDGAHIASLLMTFFYRELPD
LIRQGHLYLAQPPLYRLTQGGKTIYAMDDTDRERKLKSNFKPNAKVEVSRFKGLGEMPPGDLKQTTMDPRHRTLLKVVTR
PEDRALTSDRVEHLMGRRPELRFQFIQEHAQTVEDLDV
>Mature_678_residues
MNDDLFSAARPKREPRGRGAASAPPAPVRNDAGGEYDASAIEVLEGLEPVRRRPGMYIGGTDEGAYHHLASEILDNAMDE
AVAGHATTIDVTLEAGDWLTIRDNGRGIPVDPHPKFKDRSALEVILTTLHAGGKFSGKAYATSGGLHGVGSSVVNALAAR
MEVEVARDRAVWRQVYERGHPVTMLEKVGATQNRRGTQIRFTPDPQIFGRIHFSPARLYRLCRSKAYLFRGVTIRWSCDP
ALVRDADIPAEAVLHFPGGLADSLRDELGNAALLTEMWSGEADLPVGPDGADTGRMEWAVAFLESGSASLSSYCNTIPTP
QGGTHEAGFRAALLKGFRAWGEQRSNKRAAAVNADDVLGAIAAKLSVFIRDPQFQGQTKEKLTSSEASRLIETALRDRFE
QWLAGHPAQADNLLAFIIERAEERLRRKDLKDTPRKSATRRLRLPGKLTDCTRENAAETEIFLVEGDSAGGSAKQARNRE
TQAVLPLRGKILNVASASTEKLRANQELRDLVEALGCGIGERFDATRLRYGRIVIMTDADVDGAHIASLLMTFFYRELPD
LIRQGHLYLAQPPLYRLTQGGKTIYAMDDTDRERKLKSNFKPNAKVEVSRFKGLGEMPPGDLKQTTMDPRHRTLLKVVTR
PEDRALTSDRVEHLMGRRPELRFQFIQEHAQTVEDLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=668, Percent_Identity=24.5508982035928, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI19913406, Length=672, Percent_Identity=24.8511904761905, Blast_Score=146, Evalue=6e-35,
Organism=Escherichia coli, GI1789408, Length=647, Percent_Identity=42.967542503864, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=42.8063943161634, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17535065, Length=599, Percent_Identity=23.5392320534224, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=565, Percent_Identity=23.716814159292, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=256, Percent_Identity=25.390625, Blast_Score=86, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=628, Percent_Identity=24.6815286624204, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI17136538, Length=603, Percent_Identity=23.5489220563847, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74794; Mature: 74794

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDDLFSAARPKREPRGRGAASAPPAPVRNDAGGEYDASAIEVLEGLEPVRRRPGMYIGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCEEECC
TDEGAYHHLASEILDNAMDEAVAGHATTIDVTLEAGDWLTIRDNGRGIPVDPHPKFKDRS
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHH
ALEVILTTLHAGGKFSGKAYATSGGLHGVGSSVVNALAARMEVEVARDRAVWRQVYERGH
HHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVTMLEKVGATQNRRGTQIRFTPDPQIFGRIHFSPARLYRLCRSKAYLFRGVTIRWSCDP
CCHHHHHCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCH
ALVRDADIPAEAVLHFPGGLADSLRDELGNAALLTEMWSGEADLPVGPDGADTGRMEWAV
HHEECCCCCHHHHEECCCCHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCHHHHH
AFLESGSASLSSYCNTIPTPQGGTHEAGFRAALLKGFRAWGEQRSNKRAAAVNADDVLGA
HHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHH
IAAKLSVFIRDPQFQGQTKEKLTSSEASRLIETALRDRFEQWLAGHPAQADNLLAFIIER
HHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AEERLRRKDLKDTPRKSATRRLRLPGKLTDCTRENAAETEIFLVEGDSAGGSAKQARNRE
HHHHHHHCCCCCCCHHHHHHEECCCCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHCCCC
TQAVLPLRGKILNVASASTEKLRANQELRDLVEALGCGIGERFDATRLRYGRIVIMTDAD
CEEECCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECEEEEEECCC
VDGAHIASLLMTFFYRELPDLIRQGHLYLAQPPLYRLTQGGKTIYAMDDTDRERKLKSNF
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCEEEEECCCCHHHHHHHCC
KPNAKVEVSRFKGLGEMPPGDLKQTTMDPRHRTLLKVVTRPEDRALTSDRVEHLMGRRPE
CCCCEEEHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
LRFQFIQEHAQTVEDLDV
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDDLFSAARPKREPRGRGAASAPPAPVRNDAGGEYDASAIEVLEGLEPVRRRPGMYIGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCEEECC
TDEGAYHHLASEILDNAMDEAVAGHATTIDVTLEAGDWLTIRDNGRGIPVDPHPKFKDRS
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHH
ALEVILTTLHAGGKFSGKAYATSGGLHGVGSSVVNALAARMEVEVARDRAVWRQVYERGH
HHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVTMLEKVGATQNRRGTQIRFTPDPQIFGRIHFSPARLYRLCRSKAYLFRGVTIRWSCDP
CCHHHHHCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCH
ALVRDADIPAEAVLHFPGGLADSLRDELGNAALLTEMWSGEADLPVGPDGADTGRMEWAV
HHEECCCCCHHHHEECCCCHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCHHHHH
AFLESGSASLSSYCNTIPTPQGGTHEAGFRAALLKGFRAWGEQRSNKRAAAVNADDVLGA
HHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHH
IAAKLSVFIRDPQFQGQTKEKLTSSEASRLIETALRDRFEQWLAGHPAQADNLLAFIIER
HHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AEERLRRKDLKDTPRKSATRRLRLPGKLTDCTRENAAETEIFLVEGDSAGGSAKQARNRE
HHHHHHHCCCCCCCHHHHHHEECCCCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHCCCC
TQAVLPLRGKILNVASASTEKLRANQELRDLVEALGCGIGERFDATRLRYGRIVIMTDAD
CEEECCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECEEEEEECCC
VDGAHIASLLMTFFYRELPDLIRQGHLYLAQPPLYRLTQGGKTIYAMDDTDRERKLKSNF
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCEEEEECCCCHHHHHHHCC
KPNAKVEVSRFKGLGEMPPGDLKQTTMDPRHRTLLKVVTRPEDRALTSDRVEHLMGRRPE
CCCCEEEHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
LRFQFIQEHAQTVEDLDV
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]