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The map label for this gene is sucB [H]

Identifier: 162147212

GI number: 162147212

Start: 1419528

End: 1420958

Strand: Direct

Name: sucB [H]

Synonym: GDI_1417

Alternate gene names: 162147212

Gene position: 1419528-1420958 (Clockwise)

Preceding gene: 162147211

Following gene: 162147213

Centisome position: 35.99

GC content: 68.13

Gene sequence:

>1431_bases
ATGGCGGTGTCGTTCCGGCTTCAGTCCGGTTATGTTGAAAGGTGCACAAGCCGGCAGACGACGGGCGAGGGGCATGCGAG
CCATGTCCTGGTCGGGGGCATGCGGTCCCGGTCCAGCGCGCGCGGATCTGTCCGGCAGGGGGAATGGAAAGAAACGATGT
CTGCCGAGATCAAGGTCCCGACTCTGGGCGAGAGCGTGACGACCGCCACGATTGCGAAATGGCTGAAGAAGCCCGGTGAA
GCGGTGACGGCCGACGAACCGGTGGTGGAACTGGAGACCGACAAGGTCAGCGTCGAGGTCGCGGCGCCCGAAGCCGGCGT
GATGGGCCCGCAACTGGTGGCCGAGGGTGACGAGGTCGAGGTCGGCACCGTCCTGGCCTCGGTCGAGGCCGGCAGCGGCG
CCGCCGCCAAGCCCGCGGCCGCTGCCCCGGCGCCGAAGAAGGCCGCCGAGGCCCCGAAGGCCCCGGCCGGCGTGCAGGCC
CAGCCGACCACCAGCGGCCCCGTCGCCCGTCCCGCGACGCCGCCGTCCGACGTCGCGGCCCAGGGGGCGGCCCATGCGCC
GATGCCGTCGGCCCAGAAGATGATGACCGAAAAGGGCGTGACGACCGCCGAAATCGGAATCGGCACCGGCAAGGACGGAC
GCGTGACCAAGGGTGATGTCCTGGCCTTCCTGTCGCAGCCGCCGGTGGCCAAGGCCGCCGCGGCCCCGGCCGCCCCGCGC
ACCGACGACCCGCGCGAGGAACGGGTGAAGATGACCCGCCTGCGCCGGACCATCGCCCGCCGCCTGAAGGACGCGCAGAA
CACCGCCGCCCTGCTGACCACCTTCAACGAGGTGGACATGTCGGCGGCCAAGGCGATGCGCGCCGAATATCGCGATCTGT
TCGAGAAGAAGAACGGCGGCGTGAAGCTGGGCTTCATGTCGATCTTCGCCAAGGCGGCCATCGCGGCGCTGAAGGAATTC
CCGGCGATCAACGCGGAAATCGACGGCGACGACGTGATCTATCGCGAATTCATCAATCTCGGCATCGCGGTCGGCGGCCC
GAACGGGCTGGTCGTGCCGGTCATCCGCGACGCGGACAAGCTGAACTTCGCGGGGATCGAAAGCGCGATCGCGGGCTTCG
GCAAGCGTGCGCGTGACGGGTCGCTGAAACTGGACGAACTGTCGGGCGGCACGTTCTCGATCACCAACGGTGGCATCTAC
GGTTCGCTGATGTCCACGCCGATCGTCAATGCCCCGCAGTCGGGCATCCTGGGCATGCATGCCATCCAGGACCGTCCGGT
CGCCGTGAACGGCCAGGTCGTGATCCGGCCGATGATGTACATCGCCCTGACCTACGATCACCGCATCGTCGACGGCAAGG
AGGCTGTCAGCTTCCTGGTGCGCGTCAAGCAGAACGTGGAAGACCCGCGTCGTCTGCTGCTCGAGGTCTGA

Upstream 100 bases:

>100_bases
GTCCATGCCAGCGAGCAGGCGGCCCGGGTCGCCCAGGCGCTGGGCGTCGGCTTGACGGAACGCCCCGGCCGCGGGGCCGG
GGCATTGAAAACTGGCCGGA

Downstream 100 bases:

>100_bases
TGCGCCGGGCCGGGTCGGGTTCCTAATCCCAGCCCGGCCCGCCGATCCATCCGACCCATCGAACGCAGGACAGAAACATG
ACCATTGAAATCAAGGTGCC

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAVSFRLQSGYVERCTSRQTTGEGHASHVLVGGMRSRSSARGSVRQGEWKETMSAEIKVPTLGESVTTATIAKWLKKPGE
AVTADEPVVELETDKVSVEVAAPEAGVMGPQLVAEGDEVEVGTVLASVEAGSGAAAKPAAAAPAPKKAAEAPKAPAGVQA
QPTTSGPVARPATPPSDVAAQGAAHAPMPSAQKMMTEKGVTTAEIGIGTGKDGRVTKGDVLAFLSQPPVAKAAAAPAAPR
TDDPREERVKMTRLRRTIARRLKDAQNTAALLTTFNEVDMSAAKAMRAEYRDLFEKKNGGVKLGFMSIFAKAAIAALKEF
PAINAEIDGDDVIYREFINLGIAVGGPNGLVVPVIRDADKLNFAGIESAIAGFGKRARDGSLKLDELSGGTFSITNGGIY
GSLMSTPIVNAPQSGILGMHAIQDRPVAVNGQVVIRPMMYIALTYDHRIVDGKEAVSFLVRVKQNVEDPRRLLLEV

Sequences:

>Translated_476_residues
MAVSFRLQSGYVERCTSRQTTGEGHASHVLVGGMRSRSSARGSVRQGEWKETMSAEIKVPTLGESVTTATIAKWLKKPGE
AVTADEPVVELETDKVSVEVAAPEAGVMGPQLVAEGDEVEVGTVLASVEAGSGAAAKPAAAAPAPKKAAEAPKAPAGVQA
QPTTSGPVARPATPPSDVAAQGAAHAPMPSAQKMMTEKGVTTAEIGIGTGKDGRVTKGDVLAFLSQPPVAKAAAAPAAPR
TDDPREERVKMTRLRRTIARRLKDAQNTAALLTTFNEVDMSAAKAMRAEYRDLFEKKNGGVKLGFMSIFAKAAIAALKEF
PAINAEIDGDDVIYREFINLGIAVGGPNGLVVPVIRDADKLNFAGIESAIAGFGKRARDGSLKLDELSGGTFSITNGGIY
GSLMSTPIVNAPQSGILGMHAIQDRPVAVNGQVVIRPMMYIALTYDHRIVDGKEAVSFLVRVKQNVEDPRRLLLEV
>Mature_475_residues
AVSFRLQSGYVERCTSRQTTGEGHASHVLVGGMRSRSSARGSVRQGEWKETMSAEIKVPTLGESVTTATIAKWLKKPGEA
VTADEPVVELETDKVSVEVAAPEAGVMGPQLVAEGDEVEVGTVLASVEAGSGAAAKPAAAAPAPKKAAEAPKAPAGVQAQ
PTTSGPVARPATPPSDVAAQGAAHAPMPSAQKMMTEKGVTTAEIGIGTGKDGRVTKGDVLAFLSQPPVAKAAAAPAAPRT
DDPREERVKMTRLRRTIARRLKDAQNTAALLTTFNEVDMSAAKAMRAEYRDLFEKKNGGVKLGFMSIFAKAAIAALKEFP
AINAEIDGDDVIYREFINLGIAVGGPNGLVVPVIRDADKLNFAGIESAIAGFGKRARDGSLKLDELSGGTFSITNGGIYG
SLMSTPIVNAPQSGILGMHAIQDRPVAVNGQVVIRPMMYIALTYDHRIVDGKEAVSFLVRVKQNVEDPRRLLLEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=54.5454545454545, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI203098753, Length=520, Percent_Identity=27.8846153846154, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=28.2805429864253, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=27.9826464208243, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI110671329, Length=475, Percent_Identity=24.2105263157895, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=33.1325301204819, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=45.6674473067916, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1786305, Length=435, Percent_Identity=30.3448275862069, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=450, Percent_Identity=42, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=27.8280542986425, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=27.6255707762557, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=25.8899676375405, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=423, Percent_Identity=42.789598108747, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=28.5403050108932, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=59.375, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI18859875, Length=490, Percent_Identity=26.530612244898, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI20129315, Length=234, Percent_Identity=28.2051282051282, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24582497, Length=234, Percent_Identity=28.2051282051282, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 49880; Mature: 49748

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSFRLQSGYVERCTSRQTTGEGHASHVLVGGMRSRSSARGSVRQGEWKETMSAEIKVP
CEEEEEECCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCEEECC
TLGESVTTATIAKWLKKPGEAVTADEPVVELETDKVSVEVAAPEAGVMGPQLVAEGDEVE
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCHHCCCCCCEE
VGTVLASVEAGSGAAAKPAAAAPAPKKAAEAPKAPAGVQAQPTTSGPVARPATPPSDVAA
EEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QGAAHAPMPSAQKMMTEKGVTTAEIGIGTGKDGRVTKGDVLAFLSQPPVAKAAAAPAAPR
CCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHEEECCCCCHHHHCCCCCCC
TDDPREERVKMTRLRRTIARRLKDAQNTAALLTTFNEVDMSAAKAMRAEYRDLFEKKNGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VKLGFMSIFAKAAIAALKEFPAINAEIDGDDVIYREFINLGIAVGGPNGLVVPVIRDADK
EEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCCC
LNFAGIESAIAGFGKRARDGSLKLDELSGGTFSITNGGIYGSLMSTPIVNAPQSGILGMH
CCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCEEEHHHCCCCCCCCCCCCEEEH
AIQDRPVAVNGQVVIRPMMYIALTYDHRIVDGKEAVSFLVRVKQNVEDPRRLLLEV
HHCCCCEEECCCEEEEEEEEEEEEECCEECCHHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
AVSFRLQSGYVERCTSRQTTGEGHASHVLVGGMRSRSSARGSVRQGEWKETMSAEIKVP
EEEEEECCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCEEECC
TLGESVTTATIAKWLKKPGEAVTADEPVVELETDKVSVEVAAPEAGVMGPQLVAEGDEVE
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCHHCCCCCCEE
VGTVLASVEAGSGAAAKPAAAAPAPKKAAEAPKAPAGVQAQPTTSGPVARPATPPSDVAA
EEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QGAAHAPMPSAQKMMTEKGVTTAEIGIGTGKDGRVTKGDVLAFLSQPPVAKAAAAPAAPR
CCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHEEECCCCCHHHHCCCCCCC
TDDPREERVKMTRLRRTIARRLKDAQNTAALLTTFNEVDMSAAKAMRAEYRDLFEKKNGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VKLGFMSIFAKAAIAALKEFPAINAEIDGDDVIYREFINLGIAVGGPNGLVVPVIRDADK
EEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCCC
LNFAGIESAIAGFGKRARDGSLKLDELSGGTFSITNGGIYGSLMSTPIVNAPQSGILGMH
CCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCEEEHHHCCCCCCCCCCCCEEEH
AIQDRPVAVNGQVVIRPMMYIALTYDHRIVDGKEAVSFLVRVKQNVEDPRRLLLEV
HHCCCCEEECCCEEEEEEEEEEEEECCEECCHHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]