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The map label for this gene is truB

Identifier: 162147163

GI number: 162147163

Start: 1371173

End: 1372093

Strand: Direct

Name: truB

Synonym: GDI_1368

Alternate gene names: 162147163

Gene position: 1371173-1372093 (Clockwise)

Preceding gene: 162147161

Following gene: 162147164

Centisome position: 34.76

GC content: 70.14

Gene sequence:

>921_bases
ATGCGACGCAAGCGCGGACGCCCGATCGACGGCTGGCTGGTCATCGACAAGCCGTCGGGCATGACATCGACCGATGTCGT
CAACCGGGTGAAGCGCCTGTTCGACGCCCGCAAGGCGGGGCACGGCGGCACGCTGGACCCGCTGGCGACGGGGCTGCTGC
CCATCGCCTTCGGCGCGGCCACCAAGACCGTGCCCTACATCATGGACGGCACCAAGCGCTATGAATTCACCCTGCGCCTG
GGCGAGGCCCGCGACACCGACGACGCCGAGGGCGCGGTGATCGAAACGTCCGACGTCCGCCCGACCGACGATGCGTTCCG
CGCGGCGCTGCCGGCCTTTCGCGGCGACATCATGCAGGTGCCCCCCATCTATTCCGCGATCAAGGTGGCGGGCGAGCGCG
CCTATGACATGGCGCGCGAGGGCCGGGCCCCCGACCTGCCGCCCCGCCCGGCGCGGGTCGACCGGTTCGACCTGGTGGCG
CGCCCGGACGCCGACACCGCGATCTTCGCCGTCGAATCCGGCAAGGGCGTCTATATGCGCTCGCTGGCGCGCGACATCGC
GCGGGCATGCGGTACCGTGGGGCACGTGGCGGCGCTGCGCCGGCTGCGGGTCGGCCCGTTTTCCGAGGCGGATGCAATTC
TTCTGGACAAAATTGTCCCAAGCGACGACAACGCGCCTGCCTCACCGGATCTGCTGCTTCCGGTCGCGACCGCGCTGGCC
GACATCCCGGCGCTGGCCTTGACCCATGAAGAAGCGGATGCCCTGTCACACGGGCGGGCGGTCAGCCTGCTCGACCTGAT
GGGACGGATACCGGACGCCGTCGATCCCGCATGCGGGATCGTGCGGGGAATGGACGGGGCGCGCGTGATCGGGCTCTGCC
GGCTGGAAGATGGCTGGCTGCGTCCGGACCGCATGCTTTAA

Upstream 100 bases:

>100_bases
GCCAAGGGAAACCTGGCATGGCACAATCCGGCATTGCTCCCTAGTCAGGGCTCGTCTACACGCACGCCTCTTCCGAGGTT
CTGAAAAAAGGTTCTGCCCC

Downstream 100 bases:

>100_bases
CCGAATCTGATATGGAGAACACGATGTCGATTACCGCAGAGCGCCGTACGGCGCTGATCGGCGAATACCAGACTGCCGCC
ACGGATACCGGCTCGCCGGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRRKRGRPIDGWLVIDKPSGMTSTDVVNRVKRLFDARKAGHGGTLDPLATGLLPIAFGAATKTVPYIMDGTKRYEFTLRL
GEARDTDDAEGAVIETSDVRPTDDAFRAALPAFRGDIMQVPPIYSAIKVAGERAYDMAREGRAPDLPPRPARVDRFDLVA
RPDADTAIFAVESGKGVYMRSLARDIARACGTVGHVAALRRLRVGPFSEADAILLDKIVPSDDNAPASPDLLLPVATALA
DIPALALTHEEADALSHGRAVSLLDLMGRIPDAVDPACGIVRGMDGARVIGLCRLEDGWLRPDRML

Sequences:

>Translated_306_residues
MRRKRGRPIDGWLVIDKPSGMTSTDVVNRVKRLFDARKAGHGGTLDPLATGLLPIAFGAATKTVPYIMDGTKRYEFTLRL
GEARDTDDAEGAVIETSDVRPTDDAFRAALPAFRGDIMQVPPIYSAIKVAGERAYDMAREGRAPDLPPRPARVDRFDLVA
RPDADTAIFAVESGKGVYMRSLARDIARACGTVGHVAALRRLRVGPFSEADAILLDKIVPSDDNAPASPDLLLPVATALA
DIPALALTHEEADALSHGRAVSLLDLMGRIPDAVDPACGIVRGMDGARVIGLCRLEDGWLRPDRML
>Mature_306_residues
MRRKRGRPIDGWLVIDKPSGMTSTDVVNRVKRLFDARKAGHGGTLDPLATGLLPIAFGAATKTVPYIMDGTKRYEFTLRL
GEARDTDDAEGAVIETSDVRPTDDAFRAALPAFRGDIMQVPPIYSAIKVAGERAYDMAREGRAPDLPPRPARVDRFDLVA
RPDADTAIFAVESGKGVYMRSLARDIARACGTVGHVAALRRLRVGPFSEADAILLDKIVPSDDNAPASPDLLLPVATALA
DIPALALTHEEADALSHGRAVSLLDLMGRIPDAVDPACGIVRGMDGARVIGLCRLEDGWLRPDRML

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=229, Percent_Identity=35.3711790393013, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503337, Length=278, Percent_Identity=26.2589928057554, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI215599015, Length=278, Percent_Identity=26.2589928057554, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI2367200, Length=315, Percent_Identity=35.5555555555556, Blast_Score=181, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=29.2307692307692, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=204, Percent_Identity=28.921568627451, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_GLUDA (A9HF26)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601624.1
- RefSeq:   YP_002276448.1
- ProteinModelPortal:   A9HF26
- SMR:   A9HF26
- GeneID:   5792127
- GeneID:   6975500
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_2073
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK05389
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32939; Mature: 32939

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: ACT_SITE 46-46

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKRGRPIDGWLVIDKPSGMTSTDVVNRVKRLFDARKAGHGGTLDPLATGLLPIAFGAA
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
TKTVPYIMDGTKRYEFTLRLGEARDTDDAEGAVIETSDVRPTDDAFRAALPAFRGDIMQV
HHCCCCEECCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEC
PPIYSAIKVAGERAYDMAREGRAPDLPPRPARVDRFDLVARPDADTAIFAVESGKGVYMR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHH
SLARDIARACGTVGHVAALRRLRVGPFSEADAILLDKIVPSDDNAPASPDLLLPVATALA
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
DIPALALTHEEADALSHGRAVSLLDLMGRIPDAVDPACGIVRGMDGARVIGLCRLEDGWL
HCCHHEEECHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCC
RPDRML
CCCCCC
>Mature Secondary Structure
MRRKRGRPIDGWLVIDKPSGMTSTDVVNRVKRLFDARKAGHGGTLDPLATGLLPIAFGAA
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
TKTVPYIMDGTKRYEFTLRLGEARDTDDAEGAVIETSDVRPTDDAFRAALPAFRGDIMQV
HHCCCCEECCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEC
PPIYSAIKVAGERAYDMAREGRAPDLPPRPARVDRFDLVARPDADTAIFAVESGKGVYMR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHH
SLARDIARACGTVGHVAALRRLRVGPFSEADAILLDKIVPSDDNAPASPDLLLPVATALA
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
DIPALALTHEEADALSHGRAVSLLDLMGRIPDAVDPACGIVRGMDGARVIGLCRLEDGWL
HCCHHEEECHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCC
RPDRML
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA