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The map label for this gene is nusA [H]

Identifier: 162147158

GI number: 162147158

Start: 1364625

End: 1366196

Strand: Direct

Name: nusA [H]

Synonym: GDI_1363

Alternate gene names: 162147158

Gene position: 1364625-1366196 (Clockwise)

Preceding gene: 162147157

Following gene: 162147159

Centisome position: 34.6

GC content: 66.98

Gene sequence:

>1572_bases
ATGGATACGTCCGTTTCCCGTCCCGAACTGCTGCTGGTGGCCGATGCCGTCGCGCGTGAGAAGGCGATCGACCGGGAGGA
GGTTCTGGAGGCGATGGAACAGGCCATCCAGAAGGCCGGCCGCGCCAAGTACGGGCACGAAAAGGATATCCGCGCGACCA
TCGACCGCAAGACCGGCGACGTCCGCCTGTCCCGCTGGACCGAGGCCGTCGAGGAGGTGGAGAACGAGGAAACCCAGATC
CCGCTCCACATCGCCCGCAAGTTCAAGCCCGAGATCCAGCTGGGCGAACATCTGGTCGATCCGCTGCCGCCGATCGATTT
CGGCCGCATCGCGGCGCAGACCGCCAAGCAGGTGATCGTCCAGCGCGTGCGCGAATACGAGCGCAAGCGCCAGTATGACG
AATTCAAGGACCGCGTGGGCGAGATCGTGAACGGCACGGTCAAGCGCACCGAATACGGAAACCTGATGGTCGAGATCGGC
AGTTCCGAGGCGCTGCTGCGCCGGGACGAGCTGATCCCCCGCGAAAGCTTCCGCAATTCGGACCGCGTGCGCGCCTATAT
CTATGATGTGCGTGACGAGCCGCGCGGGCCGCAGATCTTCCTGTCGCGCACCCATCCCGCCTTCCTGGCGAAGCTGTTCG
CTCAGGAAGTGCCGGAAATCTACGACGGCATCATCGAAATCAAGGCCGTCGCCCGCGACCCGGGATCGCGCGCCAAGATG
GCGGTGATTTCCCGCGACGCGTCGATCGACCCGGTGGGCGCCTGCGTGGGCATGCGCGGATCGCGCGTCCAGGCGGTGGT
GCAGGAACTGCAGGGCGAGAAGATCGACATCATTCCCTGGAGCCCGCAGGCCGCGACCTTCGTGGTCAACGCGCTGGCGC
CGGCGGAAGTGACCAAGGTCGTGATGGACGAGGAAGCTGGCCGGGTCGAGGTCGTGGTGCCTGACGAGCAGCTCAGCCTG
GCGATCGGCCGGCGCGGGCAGAATGTCCGCCTGGCCAGCCAGCTCACCCGCTGGGACATCGACATCCTGACCGAGGCCGA
GGAATCGGAACGCCGGCAGGAAGAATTCCGCCGTCGCAGCGGCCTGTTCGTCGAGGCGCTGGACGTGGACGACGTCATCG
CCGGCCTGCTGGTGACCGAAGGCTTCCATTCGATCGAGGAACTGGCCTATGCCGACCCCGACGAACTGGCCGAGATCGAG
GGCTTCGACGAGGACGTGGCCGGCGAACTGGTCCGCCGGGCCGAGGGCTTCCTGGCCCGGCGCGAGGACGAGCTGGACGA
GAAGCGGCGCGGCCTCGGGGTGTCGGACGATGTCGCGGCGCTGGGCGTGTTCTCGAACCAGATGCTGGTGACGCTGGGCG
AGAAGGGTGTGAAGTCGCTGGACGACCTGGCCGACCTGGCGGGCGACGAACTGGTCGAGATCCTGGGCGGCGAGGTCATC
GACGAGGAAGCGGCGAACGAGATCATCATGGCCGCCCGCGCGCACTGGTTCGAAGGCGAGGAAGCCGCCGGGGAAGCCGC
CCGAGAAGCTTCTGGGGAGACGGCCGAAGGCCGGGAGGCGTCGGACGTCTGA

Upstream 100 bases:

>100_bases
GGAAGATACTACGGAAGCGGCCCCCGAAGGGGCCGGAAAATCCCCGAAGCCCGGCCGGCGGCCGGCGCGCAAGACTCACT
GAGACGTCCTGGAGGCTCTG

Downstream 100 bases:

>100_bases
TCACGAAGCCGCACCCGACCGGGCGATGCGAGACATGGAAACGGATGCCGACCAGGACAGGGATGACGAACGCGGGCCGC
TGCGGCGCTGCATCGTCACC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MDTSVSRPELLLVADAVAREKAIDREEVLEAMEQAIQKAGRAKYGHEKDIRATIDRKTGDVRLSRWTEAVEEVENEETQI
PLHIARKFKPEIQLGEHLVDPLPPIDFGRIAAQTAKQVIVQRVREYERKRQYDEFKDRVGEIVNGTVKRTEYGNLMVEIG
SSEALLRRDELIPRESFRNSDRVRAYIYDVRDEPRGPQIFLSRTHPAFLAKLFAQEVPEIYDGIIEIKAVARDPGSRAKM
AVISRDASIDPVGACVGMRGSRVQAVVQELQGEKIDIIPWSPQAATFVVNALAPAEVTKVVMDEEAGRVEVVVPDEQLSL
AIGRRGQNVRLASQLTRWDIDILTEAEESERRQEEFRRRSGLFVEALDVDDVIAGLLVTEGFHSIEELAYADPDELAEIE
GFDEDVAGELVRRAEGFLARREDELDEKRRGLGVSDDVAALGVFSNQMLVTLGEKGVKSLDDLADLAGDELVEILGGEVI
DEEAANEIIMAARAHWFEGEEAAGEAAREASGETAEGREASDV

Sequences:

>Translated_523_residues
MDTSVSRPELLLVADAVAREKAIDREEVLEAMEQAIQKAGRAKYGHEKDIRATIDRKTGDVRLSRWTEAVEEVENEETQI
PLHIARKFKPEIQLGEHLVDPLPPIDFGRIAAQTAKQVIVQRVREYERKRQYDEFKDRVGEIVNGTVKRTEYGNLMVEIG
SSEALLRRDELIPRESFRNSDRVRAYIYDVRDEPRGPQIFLSRTHPAFLAKLFAQEVPEIYDGIIEIKAVARDPGSRAKM
AVISRDASIDPVGACVGMRGSRVQAVVQELQGEKIDIIPWSPQAATFVVNALAPAEVTKVVMDEEAGRVEVVVPDEQLSL
AIGRRGQNVRLASQLTRWDIDILTEAEESERRQEEFRRRSGLFVEALDVDDVIAGLLVTEGFHSIEELAYADPDELAEIE
GFDEDVAGELVRRAEGFLARREDELDEKRRGLGVSDDVAALGVFSNQMLVTLGEKGVKSLDDLADLAGDELVEILGGEVI
DEEAANEIIMAARAHWFEGEEAAGEAAREASGETAEGREASDV
>Mature_523_residues
MDTSVSRPELLLVADAVAREKAIDREEVLEAMEQAIQKAGRAKYGHEKDIRATIDRKTGDVRLSRWTEAVEEVENEETQI
PLHIARKFKPEIQLGEHLVDPLPPIDFGRIAAQTAKQVIVQRVREYERKRQYDEFKDRVGEIVNGTVKRTEYGNLMVEIG
SSEALLRRDELIPRESFRNSDRVRAYIYDVRDEPRGPQIFLSRTHPAFLAKLFAQEVPEIYDGIIEIKAVARDPGSRAKM
AVISRDASIDPVGACVGMRGSRVQAVVQELQGEKIDIIPWSPQAATFVVNALAPAEVTKVVMDEEAGRVEVVVPDEQLSL
AIGRRGQNVRLASQLTRWDIDILTEAEESERRQEEFRRRSGLFVEALDVDDVIAGLLVTEGFHSIEELAYADPDELAEIE
GFDEDVAGELVRRAEGFLARREDELDEKRRGLGVSDDVAALGVFSNQMLVTLGEKGVKSLDDLADLAGDELVEILGGEVI
DEEAANEIIMAARAHWFEGEEAAGEAAREASGETAEGREASDV

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=44.6464646464646, Blast_Score=390, Evalue=1e-110,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 58218; Mature: 58218

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSVSRPELLLVADAVAREKAIDREEVLEAMEQAIQKAGRAKYGHEKDIRATIDRKTGD
CCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCC
VRLSRWTEAVEEVENEETQIPLHIARKFKPEIQLGEHLVDPLPPIDFGRIAAQTAKQVIV
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
QRVREYERKRQYDEFKDRVGEIVNGTVKRTEYGNLMVEIGSSEALLRRDELIPRESFRNS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCHHHHHHHHCCCHHHHCCC
DRVRAYIYDVRDEPRGPQIFLSRTHPAFLAKLFAQEVPEIYDGIIEIKAVARDPGSRAKM
CCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
AVISRDASIDPVGACVGMRGSRVQAVVQELQGEKIDIIPWSPQAATFVVNALAPAEVTKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VMDEEAGRVEVVVPDEQLSLAIGRRGQNVRLASQLTRWDIDILTEAEESERRQEEFRRRS
HHCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHC
GLFVEALDVDDVIAGLLVTEGFHSIEELAYADPDELAEIEGFDEDVAGELVRRAEGFLAR
CCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
REDELDEKRRGLGVSDDVAALGVFSNQMLVTLGEKGVKSLDDLADLAGDELVEILGGEVI
HHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHH
DEEAANEIIMAARAHWFEGEEAAGEAAREASGETAEGREASDV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDTSVSRPELLLVADAVAREKAIDREEVLEAMEQAIQKAGRAKYGHEKDIRATIDRKTGD
CCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCC
VRLSRWTEAVEEVENEETQIPLHIARKFKPEIQLGEHLVDPLPPIDFGRIAAQTAKQVIV
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
QRVREYERKRQYDEFKDRVGEIVNGTVKRTEYGNLMVEIGSSEALLRRDELIPRESFRNS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCHHHHHHHHCCCHHHHCCC
DRVRAYIYDVRDEPRGPQIFLSRTHPAFLAKLFAQEVPEIYDGIIEIKAVARDPGSRAKM
CCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
AVISRDASIDPVGACVGMRGSRVQAVVQELQGEKIDIIPWSPQAATFVVNALAPAEVTKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VMDEEAGRVEVVVPDEQLSLAIGRRGQNVRLASQLTRWDIDILTEAEESERRQEEFRRRS
HHCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHC
GLFVEALDVDDVIAGLLVTEGFHSIEELAYADPDELAEIEGFDEDVAGELVRRAEGFLAR
CCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
REDELDEKRRGLGVSDDVAALGVFSNQMLVTLGEKGVKSLDDLADLAGDELVEILGGEVI
HHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHH
DEEAANEIIMAARAHWFEGEEAAGEAAREASGETAEGREASDV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]