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The map label for this gene is ydgK [H]

Identifier: 162147138

GI number: 162147138

Start: 1342428

End: 1343735

Strand: Direct

Name: ydgK [H]

Synonym: GDI_1343

Alternate gene names: 162147138

Gene position: 1342428-1343735 (Clockwise)

Preceding gene: 162147137

Following gene: 162147139

Centisome position: 34.04

GC content: 69.95

Gene sequence:

>1308_bases
ATGGCGTCTTCCGCGACAGCCGCGAGATCCGACGATGCCCCTGTTTCCGCCGTCGCCGCCGGGTCCATGAGCTGGATAGC
CATCCTGCTGGGGTTCCTCACGGCCGTGGGGCCGGTATCGACGGATATCTACCTTCCCGCCTTTCCGGCGCTGGAAGCGT
CACTGCACGCGCCTGCCGGCTCGGCGGAAATGACGCTGGCCGTCTGGTTCGTCGGGCTGGCGGTCGGGCAGATCACGATG
GGCCCGGTGTCGGACCGGTTCGGCCGGCGCATGCCCATGCTGGTGGGCACGCTGGTCTATACCCTGGCCTCGGTCGGCTG
CGCCGTGGCGCCCGATATCTGGACGTTTTCCAGCTTCCGCCTGCTGGCGTCGCTGGGGGCGTCGGCCAGCCTGGTGATCC
CCAGCGCCTGCGTGCGCGACCTGTCCCACGGGAACGAGGCCGCGCGCCTGATGTCGCGGCTGGTGCTGGTCATGGGCGTG
GTGCCGATCCTGGCGCCGACCCTGGGCGGCTTCGTGCTGTCCTTCACCACCTGGCGGTCGATCTTCTGGGCGTCGGCGGT
CTACGGGCTGATCTGCACGTTCCTGATCGCGCGGGTCCTGCCGGAAACCCTGGCCGCCCACGACCGCAGCGTGATGCCCC
CGGTGGCGCTGGCCGGCCGCTACCTGACGCTGGTGCGGGACCGGGGCTTCATCACCAATGCCCTGGTGGCGGGCTTCGGG
GCGTTCATGTCGTTCACCTACCTGTCGGCGGCGCCGTCGGTCTTCATCCATCTGTTCGGGTTCTCGCCCGCGCAGTTCGG
CATGCTGTTCGGGGTGTTCGCCGTCTGCATGATCGGGGCGTCGCAACTGAACGGCGTGCTGGTCGGGCGGATCGACGCCA
CGCGCATCCTGGGCATTTCGGTCGGGACCGCCGTGCTGGGAACGGTGGCGATGACGGTGGTGTCGATCGTCATGGCCTTC
GCGATGAAGGGGGGCGTCATGCTGCATAGCCTGGCGCTGGTGCCGCTGGTCCTGACGATGATGGTGGCGCTCGGCACCAC
CGGCATCATCGGGCCGAACGCCACGGTGGGGGCCATGGCCGACCATCCGCGCCTGGCCGGCAGCGCCTCGGCGTTCGTCG
GCACGCTGCAATACGTGCTGGGCGCCGTCGCGGGCGCCATCGTGGGCATGCTGCCCGCCACCACCCCGGTGCCGATGTCC
GGCGTCATGCTGACGGGCGCCGTCATCATGCTGCTGATGGTGCTGCTGCGGCCCGCCCGCCGGCCGGGCGAGGCCACCGT
CCCGGCCGACCTTCCGATGATGCACTGA

Upstream 100 bases:

>100_bases
GCACCGCGTACGACGAATTGCAGCCTTACTGTAATTATATGATGAAAAACGACAGGCCGCTGCGATAGTACGGCCTGACG
ATCTCTAACGGAGTGCCGCC

Downstream 100 bases:

>100_bases
CGCTTGCCTCGGCGATTGCCTCGACGATTGCCTCCCTGGCGGCGGCAGGTTACAGCAGGGCACGTTTTTCACGGTCCGGA
CGGCAGGTCCGGCCCGGTTC

Product: antibiotic resistance transport membrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MASSATAARSDDAPVSAVAAGSMSWIAILLGFLTAVGPVSTDIYLPAFPALEASLHAPAGSAEMTLAVWFVGLAVGQITM
GPVSDRFGRRMPMLVGTLVYTLASVGCAVAPDIWTFSSFRLLASLGASASLVIPSACVRDLSHGNEAARLMSRLVLVMGV
VPILAPTLGGFVLSFTTWRSIFWASAVYGLICTFLIARVLPETLAAHDRSVMPPVALAGRYLTLVRDRGFITNALVAGFG
AFMSFTYLSAAPSVFIHLFGFSPAQFGMLFGVFAVCMIGASQLNGVLVGRIDATRILGISVGTAVLGTVAMTVVSIVMAF
AMKGGVMLHSLALVPLVLTMMVALGTTGIIGPNATVGAMADHPRLAGSASAFVGTLQYVLGAVAGAIVGMLPATTPVPMS
GVMLTGAVIMLLMVLLRPARRPGEATVPADLPMMH

Sequences:

>Translated_435_residues
MASSATAARSDDAPVSAVAAGSMSWIAILLGFLTAVGPVSTDIYLPAFPALEASLHAPAGSAEMTLAVWFVGLAVGQITM
GPVSDRFGRRMPMLVGTLVYTLASVGCAVAPDIWTFSSFRLLASLGASASLVIPSACVRDLSHGNEAARLMSRLVLVMGV
VPILAPTLGGFVLSFTTWRSIFWASAVYGLICTFLIARVLPETLAAHDRSVMPPVALAGRYLTLVRDRGFITNALVAGFG
AFMSFTYLSAAPSVFIHLFGFSPAQFGMLFGVFAVCMIGASQLNGVLVGRIDATRILGISVGTAVLGTVAMTVVSIVMAF
AMKGGVMLHSLALVPLVLTMMVALGTTGIIGPNATVGAMADHPRLAGSASAFVGTLQYVLGAVAGAIVGMLPATTPVPMS
GVMLTGAVIMLLMVLLRPARRPGEATVPADLPMMH
>Mature_434_residues
ASSATAARSDDAPVSAVAAGSMSWIAILLGFLTAVGPVSTDIYLPAFPALEASLHAPAGSAEMTLAVWFVGLAVGQITMG
PVSDRFGRRMPMLVGTLVYTLASVGCAVAPDIWTFSSFRLLASLGASASLVIPSACVRDLSHGNEAARLMSRLVLVMGVV
PILAPTLGGFVLSFTTWRSIFWASAVYGLICTFLIARVLPETLAAHDRSVMPPVALAGRYLTLVRDRGFITNALVAGFGA
FMSFTYLSAAPSVFIHLFGFSPAQFGMLFGVFAVCMIGASQLNGVLVGRIDATRILGISVGTAVLGTVAMTVVSIVMAFA
MKGGVMLHSLALVPLVLTMMVALGTTGIIGPNATVGAMADHPRLAGSASAFVGTLQYVLGAVAGAIVGMLPATTPVPMSG
VMLTGAVIMLLMVLLRPARRPGEATVPADLPMMH

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=398, Percent_Identity=30.1507537688442, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI48994889, Length=399, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790146, Length=233, Percent_Identity=26.1802575107296, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082312, Length=362, Percent_Identity=27.3480662983425, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324264, Length=220, Percent_Identity=27.7272727272727, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319480, Length=322, Percent_Identity=26.3975155279503, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=214, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44833; Mature: 44702

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSATAARSDDAPVSAVAAGSMSWIAILLGFLTAVGPVSTDIYLPAFPALEASLHAPAG
CCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCC
SAEMTLAVWFVGLAVGQITMGPVSDRFGRRMPMLVGTLVYTLASVGCAVAPDIWTFSSFR
CCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LLASLGASASLVIPSACVRDLSHGNEAARLMSRLVLVMGVVPILAPTLGGFVLSFTTWRS
HHHHCCCCCEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFWASAVYGLICTFLIARVLPETLAAHDRSVMPPVALAGRYLTLVRDRGFITNALVAGFG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
AFMSFTYLSAAPSVFIHLFGFSPAQFGMLFGVFAVCMIGASQLNGVLVGRIDATRILGIS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHH
VGTAVLGTVAMTVVSIVMAFAMKGGVMLHSLALVPLVLTMMVALGTTGIIGPNATVGAMA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DHPRLAGSASAFVGTLQYVLGAVAGAIVGMLPATTPVPMSGVMLTGAVIMLLMVLLRPAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
RPGEATVPADLPMMH
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSATAARSDDAPVSAVAAGSMSWIAILLGFLTAVGPVSTDIYLPAFPALEASLHAPAG
CCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCC
SAEMTLAVWFVGLAVGQITMGPVSDRFGRRMPMLVGTLVYTLASVGCAVAPDIWTFSSFR
CCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LLASLGASASLVIPSACVRDLSHGNEAARLMSRLVLVMGVVPILAPTLGGFVLSFTTWRS
HHHHCCCCCEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFWASAVYGLICTFLIARVLPETLAAHDRSVMPPVALAGRYLTLVRDRGFITNALVAGFG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
AFMSFTYLSAAPSVFIHLFGFSPAQFGMLFGVFAVCMIGASQLNGVLVGRIDATRILGIS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHH
VGTAVLGTVAMTVVSIVMAFAMKGGVMLHSLALVPLVLTMMVALGTTGIIGPNATVGAMA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DHPRLAGSASAFVGTLQYVLGAVAGAIVGMLPATTPVPMSGVMLTGAVIMLLMVLLRPAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
RPGEATVPADLPMMH
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]