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The map label for this gene is folD

Identifier: 162147100

GI number: 162147100

Start: 1305176

End: 1306111

Strand: Direct

Name: folD

Synonym: GDI_1305

Alternate gene names: 162147100

Gene position: 1305176-1306111 (Clockwise)

Preceding gene: 162147098

Following gene: 162147101

Centisome position: 33.09

GC content: 70.09

Gene sequence:

>936_bases
ATGACCGATCGAACGACCGATCCCGTGCCTGCCGAACCCGCGCCGGTTCGCCATCCCACCGCGCGCCTGATCGACGGCAA
GGCGTTCGCGGCGAACCTGACGCAGACGATCGCACGGGACGTGCAATCATTCCATGATCGTTATCGCGTCACCCCCGGGC
TGGCGGTGGTGCTGGTCGGTAACGATCCCGCCAGCGAGGTCTATGTCCGCAACAAGGCGTTGCAGACCCATCGCGCGGGC
ATGCGCTCGTTCATGCACATGCTGCCCGCCACCACGTCGCAGGCCGAGCTTCTGGCGCTGATCGGACGCCTGAACGCCGA
TCCGGAAATCCACGGCATCCTGGTGCAGTTGCCGCTGCCCGCCGGTCTGGACCCCGTCGCCGTGACCAATGCCATCCTGC
CGGACAAGGATGTGGACGGGCTGGGCGAGGTCAATGCCGGCCGCCTGGCGCTGGGCCAGCCGGGGATCGTGCCGTGCACG
CCGCTGGGCTGCCTGATGCTGCTGAAGTCGGAACTGGGCGATCTGCGCGGACTGCACGCGGTCATCATCGGCGCGTCCAA
CCTGGTCGGCCGGCCGATGGCCCGGCTGCTGCTGGCCGAGGGCTGCACGGTGACGGTCGGGCATATCGACACGCGCGACC
CCGCGGCGCTGGCGCGGCAGGGGGATATCCTGGTGGTGGCCACCGGCTGCCACGGGCTGGTGCGCGGCGACTGGATCAAG
CCGGGCGCCGCCGTGATCGACGTGGGCATCACCCGCATATCGCTGCCCAGCGGCAAGACGCGCCTGGTCGGCGACGTCGC
GTTCGACGAGGCCGTGACCCGTGCGGGATGCATCACCCCGGTGCCGGGCGGCGTCGGGCCGATGACCATCGCCTGCCTGC
TGAACAACACCCTGGCGGCGGCGCGTCGCATCGTCGGCGCGACGGACGCGGCATGA

Upstream 100 bases:

>100_bases
GGAACGACGTTGTCGCTGCTGAAATTTTTGCGTATCTGCTCTACCACCCTGGCCCCCTGTCCTATGCCATCTGGCCCATG
CCCGCTGTCCGGAGCGCCCC

Downstream 100 bases:

>100_bases
CCCGGATTTCGGTCGAACTGATCCCCCGCGACGCCGAAGCCCTGCTGCGCGACGCGGCCGAGGTCCGGCAGCATTTCCCG
CTGGCCGACACGCTGAACGT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTDRTTDPVPAEPAPVRHPTARLIDGKAFAANLTQTIARDVQSFHDRYRVTPGLAVVLVGNDPASEVYVRNKALQTHRAG
MRSFMHMLPATTSQAELLALIGRLNADPEIHGILVQLPLPAGLDPVAVTNAILPDKDVDGLGEVNAGRLALGQPGIVPCT
PLGCLMLLKSELGDLRGLHAVIIGASNLVGRPMARLLLAEGCTVTVGHIDTRDPAALARQGDILVVATGCHGLVRGDWIK
PGAAVIDVGITRISLPSGKTRLVGDVAFDEAVTRAGCITPVPGGVGPMTIACLLNNTLAAARRIVGATDAA

Sequences:

>Translated_311_residues
MTDRTTDPVPAEPAPVRHPTARLIDGKAFAANLTQTIARDVQSFHDRYRVTPGLAVVLVGNDPASEVYVRNKALQTHRAG
MRSFMHMLPATTSQAELLALIGRLNADPEIHGILVQLPLPAGLDPVAVTNAILPDKDVDGLGEVNAGRLALGQPGIVPCT
PLGCLMLLKSELGDLRGLHAVIIGASNLVGRPMARLLLAEGCTVTVGHIDTRDPAALARQGDILVVATGCHGLVRGDWIK
PGAAVIDVGITRISLPSGKTRLVGDVAFDEAVTRAGCITPVPGGVGPMTIACLLNNTLAAARRIVGATDAA
>Mature_310_residues
TDRTTDPVPAEPAPVRHPTARLIDGKAFAANLTQTIARDVQSFHDRYRVTPGLAVVLVGNDPASEVYVRNKALQTHRAGM
RSFMHMLPATTSQAELLALIGRLNADPEIHGILVQLPLPAGLDPVAVTNAILPDKDVDGLGEVNAGRLALGQPGIVPCTP
LGCLMLLKSELGDLRGLHAVIIGASNLVGRPMARLLLAEGCTVTVGHIDTRDPAALARQGDILVVATGCHGLVRGDWIKP
GAAVIDVGITRISLPSGKTRLVGDVAFDEAVTRAGCITPVPGGVGPMTIACLLNNTLAAARRIVGATDAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=43.6860068259386, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI222418558, Length=301, Percent_Identity=43.1893687707641, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI94721354, Length=309, Percent_Identity=41.4239482200647, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=26.7676767676768, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=53.2142857142857, Blast_Score=260, Evalue=9e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=40.2061855670103, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=43.2432432432432, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=296, Percent_Identity=41.8918918918919, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.9591836734694, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.9591836734694, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI62472483, Length=302, Percent_Identity=43.7086092715232, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45551871, Length=302, Percent_Identity=43.7086092715232, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=43.8356164383562, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=43.8356164383562, Blast_Score=218, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_GLUDA (A9HEL1)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601561.1
- RefSeq:   YP_002276390.1
- ProteinModelPortal:   A9HEL1
- SMR:   A9HEL1
- GeneID:   5789639
- GeneID:   6975441
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_2015
- HOGENOM:   HBG328751
- OMA:   GINHIHD
- ProtClustDB:   PRK14188
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32340; Mature: 32209

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRTTDPVPAEPAPVRHPTARLIDGKAFAANLTQTIARDVQSFHDRYRVTPGLAVVLVG
CCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEC
NDPASEVYVRNKALQTHRAGMRSFMHMLPATTSQAELLALIGRLNADPEIHGILVQLPLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCC
AGLDPVAVTNAILPDKDVDGLGEVNAGRLALGQPGIVPCTPLGCLMLLKSELGDLRGLHA
CCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
VIIGASNLVGRPMARLLLAEGCTVTVGHIDTRDPAALARQGDILVVATGCHGLVRGDWIK
EEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCC
PGAAVIDVGITRISLPSGKTRLVGDVAFDEAVTRAGCITPVPGGVGPMTIACLLNNTLAA
CCCEEEEECEEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ARRIVGATDAA
HHHHHCCCCCC
>Mature Secondary Structure 
TDRTTDPVPAEPAPVRHPTARLIDGKAFAANLTQTIARDVQSFHDRYRVTPGLAVVLVG
CCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEC
NDPASEVYVRNKALQTHRAGMRSFMHMLPATTSQAELLALIGRLNADPEIHGILVQLPLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCC
AGLDPVAVTNAILPDKDVDGLGEVNAGRLALGQPGIVPCTPLGCLMLLKSELGDLRGLHA
CCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
VIIGASNLVGRPMARLLLAEGCTVTVGHIDTRDPAALARQGDILVVATGCHGLVRGDWIK
EEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCC
PGAAVIDVGITRISLPSGKTRLVGDVAFDEAVTRAGCITPVPGGVGPMTIACLLNNTLAA
CCCEEEEECEEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ARRIVGATDAA
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA