Click here to switch to the map view.

The map label for this gene is acrA [H]

Identifier: 162147079

GI number: 162147079

Start: 1281207

End: 1282376

Strand: Direct

Name: acrA [H]

Synonym: GDI_1284

Alternate gene names: 162147079

Gene position: 1281207-1282376 (Clockwise)

Preceding gene: 162147075

Following gene: 162147080

Centisome position: 32.48

GC content: 70.68

Gene sequence:

>1170_bases
ATGACGCCCCCTCGTCCTTATCGTGCCGTCCTTCTGGCCGCCTGTGCGTCCCTGGCGCTGGCCGGATGCAACCGCAAGGC
CGCGCCCCTCGCCCCGCCGCCGCAGAGGGTCGAGGTCCTGACCCTGCGCGCGGCGCCGGTCGGCGTCACCACCGACCTGC
CCGGACGCACCGAAGCGGTCGAAATCGCCCAGGTCCGCCCGCAGGTCGCGGGCGTGATCCAGAAGCGCCTGTTCGTCGAA
GGCTCGGACGTCACGGCCGGGCAGCAGCTGTACCAGATCGATCCGTCCGCCTATCAGGCGACCTACGACATGGCGAAGGC
GCAATGGCTGCACGCCAAGGCCGCGCGGGTGACGGCACAGGCGCGGCTGGACCGCTACGGCCCGCTGGCGCAGGCCCACG
CCGTCAGCCGGCAGGATTACGATGACGCCCTGGCGGCGGCGCGCGAGGCCGATGCCGACATCGCCCAGGGCCGTGCCAAC
ATGGACCGCGCGGCGGTCGACCTGGGCTATACCCGCGTGCTGTCGCCGATTTCCGGGCGCATCGGCCGATCGCTGATGAC
GGTGGGTGCCCTAGCGGTCGTGGGCCAGTCCAGCAGCCTGTCGGTCGTCACCCGGCTGGACCCGATCTATGTCGACGTGA
ACCTGGCGGCGACGCGGCTTCTGGAACTGCGGCGCGAACTGGCCCAGGGACGGCTGAGCCGCGCGGGCGACAATGCCGCC
ACCGTCAGCCTGACGCTGGAGGACGGCAGCCGCTATGAAACGCAGGGCCGCCTCGAATTCTCCGAGGTGAACGTGGACGA
GACGACGGGCACCGTGGTGGTCCGCGCCATCTTCCCCAACGCCGCCCATATGCTGCTGCCGGGCATGTACGTGCATGCCG
AACTGCAGGAAGGCGTCGATCCCAAGGCCCTGCGCGTGCCGCAGGTGGCGGTGGTGCGCACCCCGCACGGTGACCCCATG
GTCATGGTCGTGGGCGCGGACAGCAAGGTGGCGACGCGCATGATCACGACCGGGGCCGCCGTCGGCACAGACTGGATCGT
GACCGACGGGCTGAAGCCCGGCGATCGGGTAGTGGTCAGCGGCCTGCAGAAGATCCACCCCGGCGATACCGTCGCACCCA
CCGAGGTCGCGCAGGGCGGTGCTCCGCAGGATCAGGGCAAGGCGGGGTAA

Upstream 100 bases:

>100_bases
ATGGGTCGGTTTCGCGGGCCGGATTCCCCCCGTGATTCATCCTCGTTCGACCCGGCGTACCACGCTTTCACACCACGCTT
TCTGCAGGGTTGAACCACGC

Downstream 100 bases:

>100_bases
CGATCCATGTCGCGCTTCTTCATCGACCGGCCCGTCTTCGCATGGGTCATCGGCCTGGTGATCATGCTGGTGGGGGCCGT
CGCCGTCTTCCGCCTGCCGA

Product: acriflavine resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTPPRPYRAVLLAACASLALAGCNRKAAPLAPPPQRVEVLTLRAAPVGVTTDLPGRTEAVEIAQVRPQVAGVIQKRLFVE
GSDVTAGQQLYQIDPSAYQATYDMAKAQWLHAKAARVTAQARLDRYGPLAQAHAVSRQDYDDALAAAREADADIAQGRAN
MDRAAVDLGYTRVLSPISGRIGRSLMTVGALAVVGQSSSLSVVTRLDPIYVDVNLAATRLLELRRELAQGRLSRAGDNAA
TVSLTLEDGSRYETQGRLEFSEVNVDETTGTVVVRAIFPNAAHMLLPGMYVHAELQEGVDPKALRVPQVAVVRTPHGDPM
VMVVGADSKVATRMITTGAAVGTDWIVTDGLKPGDRVVVSGLQKIHPGDTVAPTEVAQGGAPQDQGKAG

Sequences:

>Translated_389_residues
MTPPRPYRAVLLAACASLALAGCNRKAAPLAPPPQRVEVLTLRAAPVGVTTDLPGRTEAVEIAQVRPQVAGVIQKRLFVE
GSDVTAGQQLYQIDPSAYQATYDMAKAQWLHAKAARVTAQARLDRYGPLAQAHAVSRQDYDDALAAAREADADIAQGRAN
MDRAAVDLGYTRVLSPISGRIGRSLMTVGALAVVGQSSSLSVVTRLDPIYVDVNLAATRLLELRRELAQGRLSRAGDNAA
TVSLTLEDGSRYETQGRLEFSEVNVDETTGTVVVRAIFPNAAHMLLPGMYVHAELQEGVDPKALRVPQVAVVRTPHGDPM
VMVVGADSKVATRMITTGAAVGTDWIVTDGLKPGDRVVVSGLQKIHPGDTVAPTEVAQGGAPQDQGKAG
>Mature_388_residues
TPPRPYRAVLLAACASLALAGCNRKAAPLAPPPQRVEVLTLRAAPVGVTTDLPGRTEAVEIAQVRPQVAGVIQKRLFVEG
SDVTAGQQLYQIDPSAYQATYDMAKAQWLHAKAARVTAQARLDRYGPLAQAHAVSRQDYDDALAAAREADADIAQGRANM
DRAAVDLGYTRVLSPISGRIGRSLMTVGALAVVGQSSSLSVVTRLDPIYVDVNLAATRLLELRRELAQGRLSRAGDNAAT
VSLTLEDGSRYETQGRLEFSEVNVDETTGTVVVRAIFPNAAHMLLPGMYVHAELQEGVDPKALRVPQVAVVRTPHGDPMV
MVVGADSKVATRMITTGAAVGTDWIVTDGLKPGDRVVVSGLQKIHPGDTVAPTEVAQGGAPQDQGKAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=51.4905149051491, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1789665, Length=358, Percent_Identity=47.4860335195531, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1789929, Length=361, Percent_Identity=42.6592797783933, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI87082036, Length=333, Percent_Identity=27.3273273273273, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41112; Mature: 40981

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPRPYRAVLLAACASLALAGCNRKAAPLAPPPQRVEVLTLRAAPVGVTTDLPGRTEAV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCHHH
EIAQVRPQVAGVIQKRLFVEGSDVTAGQQLYQIDPSAYQATYDMAKAQWLHAKAARVTAQ
HHHHHCHHHHHHHHHHHEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
ARLDRYGPLAQAHAVSRQDYDDALAAAREADADIAQGRANMDRAAVDLGYTRVLSPISGR
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IGRSLMTVGALAVVGQSSSLSVVTRLDPIYVDVNLAATRLLELRRELAQGRLSRAGDNAA
HHHHHHHHHHHHEECCCCCCEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEE
TVSLTLEDGSRYETQGRLEFSEVNVDETTGTVVVRAIFPNAAHMLLPGMYVHAELQEGVD
EEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHCCCEEEEEHHHCCCC
PKALRVPQVAVVRTPHGDPMVMVVGADSKVATRMITTGAAVGTDWIVTDGLKPGDRVVVS
CCCEECCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHH
GLQKIHPGDTVAPTEVAQGGAPQDQGKAG
HHHHCCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TPPRPYRAVLLAACASLALAGCNRKAAPLAPPPQRVEVLTLRAAPVGVTTDLPGRTEAV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCHHH
EIAQVRPQVAGVIQKRLFVEGSDVTAGQQLYQIDPSAYQATYDMAKAQWLHAKAARVTAQ
HHHHHCHHHHHHHHHHHEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
ARLDRYGPLAQAHAVSRQDYDDALAAAREADADIAQGRANMDRAAVDLGYTRVLSPISGR
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IGRSLMTVGALAVVGQSSSLSVVTRLDPIYVDVNLAATRLLELRRELAQGRLSRAGDNAA
HHHHHHHHHHHHEECCCCCCEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEE
TVSLTLEDGSRYETQGRLEFSEVNVDETTGTVVVRAIFPNAAHMLLPGMYVHAELQEGVD
EEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHCCCEEEEEHHHCCCC
PKALRVPQVAVVRTPHGDPMVMVVGADSKVATRMITTGAAVGTDWIVTDGLKPGDRVVVS
CCCEECCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHH
GLQKIHPGDTVAPTEVAQGGAPQDQGKAG
HHHHCCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]