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The map label for this gene is metK

Identifier: 162147054

GI number: 162147054

Start: 1254195

End: 1255382

Strand: Reverse

Name: metK

Synonym: GDI_1259

Alternate gene names: 162147054

Gene position: 1255382-1254195 (Counterclockwise)

Preceding gene: 162147055

Following gene: 162147053

Centisome position: 31.83

GC content: 67.51

Gene sequence:

>1188_bases
ATGCGTAACAAAGGCGATTTTCTGTTCACGTCGGAATCGGTATCCGAAGGCCACCCCGACAAGGTTGCCGACCGGATCAG
CGATACGGTCCTGGACGCCTACCTGGCGGCCGATGGTGAATCCCGCGTCGCCTGCGAAACACTGGTGACGACGAACCGCG
TGGTCCTGGCGGGCGAAGTTCGCGGCCCGTCCTCCGTCACCCCCGAGGCGCTGATCGAGGGCGCGCGCGCCGCGATCCGC
GACATCGGCTATGACCAGGCCGGCTTCTCGTGGAAGAACGCCCAGATCGAATCCTACCTGCACGCGCAGTCCGCCGATAT
CGCCGTCGGCGTCGACAGCGCCGGCGAGAAGGACGAGGGTGCGGGCGACCAGGGCATCATGTTCGGCTTCGCGACGCGCG
AGACCGAAACGCTGATGCCCGCGCCGCTGTTCTATGCCCACGGCATCCTGCATCGCATCCGCGACCTGCGCAAAGCGGGC
GACGCGCGCGTGGCCATGCTGCAGCCCGACGCCAAGAGCCAGGTCACGCTGCGCTATGTCGACGGCCGCCCGGTCGGCGC
GACCTCGGTCGTCATTTCCACCCAGCACGACGAGGGCGCCAGCCAGGCCGCGATCCGCGAGGCCCTGCGCGACGTGGTGC
AGGACGTGCTGCCCGAAGGCTGGATGTGCCCGGAAGACGAATTCTACGCCAACCCGACCGGCGTGTTCGTCATCGGCGGT
CCGGACGGCGATTGCGGTCTGACCGGGCGCAAGATCATCGTCGACACCTACGGCGGCGCGGCCCCGCATGGCGGCGGCGC
ATTCTCGGGCAAGGACCCGACCAAGGTCGACCGTTCGGCGGCCTATGCCTGCCGCTACCTAGCCAAGAACGTGGTTGCGG
CCGGCCTGGCCGATCGCTGTACGCTGCAGATCTCCTACGCCATCGGCGTGTCGCATCCGCTGTCGGTCTATGTGGACCTG
GACGGCACCGGCAAGGACGTGGACGAGGCGAAGCTGGGCAGCGTCCTGCGCGAAGTCATGAACCTGACGCCGCGCGGCAT
CCGCCAGCATCTGCGCCTGAACCGTCCGATCTATACCGAGACCTCGGCCTATGGCCATTTCGGCCGCACGCCGGATGAAG
CCCGCGACAATTTCACGTGGGAGAAGACCGACCTGGTGGACGCGCTGCGCGGCGCGTTCAACCGCTGA

Upstream 100 bases:

>100_bases
ATACTTGACGCTCTGGGGGTATTGGGTCAAACGGACCCGCCCGGACCGCGCATGCGTGGCGGCGTCACCCTTTCCTTCGT
TCGAGATAGACAGGACCATC

Downstream 100 bases:

>100_bases
TCGCGGCAGCCAAGGTCGTATGACGGTCCCGCAGGCACCTGACGGTACGGTATCGGGGGTCAAGGCTTCGCCTGACCGGC
TGTATGGCCGTCAGCGCGGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRNKGDFLFTSESVSEGHPDKVADRISDTVLDAYLAADGESRVACETLVTTNRVVLAGEVRGPSSVTPEALIEGARAAIR
DIGYDQAGFSWKNAQIESYLHAQSADIAVGVDSAGEKDEGAGDQGIMFGFATRETETLMPAPLFYAHGILHRIRDLRKAG
DARVAMLQPDAKSQVTLRYVDGRPVGATSVVISTQHDEGASQAAIREALRDVVQDVLPEGWMCPEDEFYANPTGVFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLADRCTLQISYAIGVSHPLSVYVDL
DGTGKDVDEAKLGSVLREVMNLTPRGIRQHLRLNRPIYTETSAYGHFGRTPDEARDNFTWEKTDLVDALRGAFNR

Sequences:

>Translated_395_residues
MRNKGDFLFTSESVSEGHPDKVADRISDTVLDAYLAADGESRVACETLVTTNRVVLAGEVRGPSSVTPEALIEGARAAIR
DIGYDQAGFSWKNAQIESYLHAQSADIAVGVDSAGEKDEGAGDQGIMFGFATRETETLMPAPLFYAHGILHRIRDLRKAG
DARVAMLQPDAKSQVTLRYVDGRPVGATSVVISTQHDEGASQAAIREALRDVVQDVLPEGWMCPEDEFYANPTGVFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLADRCTLQISYAIGVSHPLSVYVDL
DGTGKDVDEAKLGSVLREVMNLTPRGIRQHLRLNRPIYTETSAYGHFGRTPDEARDNFTWEKTDLVDALRGAFNR
>Mature_395_residues
MRNKGDFLFTSESVSEGHPDKVADRISDTVLDAYLAADGESRVACETLVTTNRVVLAGEVRGPSSVTPEALIEGARAAIR
DIGYDQAGFSWKNAQIESYLHAQSADIAVGVDSAGEKDEGAGDQGIMFGFATRETETLMPAPLFYAHGILHRIRDLRKAG
DARVAMLQPDAKSQVTLRYVDGRPVGATSVVISTQHDEGASQAAIREALRDVVQDVLPEGWMCPEDEFYANPTGVFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLADRCTLQISYAIGVSHPLSVYVDL
DGTGKDVDEAKLGSVLREVMNLTPRGIRQHLRLNRPIYTETSAYGHFGRTPDEARDNFTWEKTDLVDALRGAFNR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=48.062015503876, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI4557737, Length=387, Percent_Identity=47.0284237726098, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=55.1546391752577, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17538498, Length=384, Percent_Identity=48.1770833333333, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25145633, Length=388, Percent_Identity=47.680412371134, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17538494, Length=384, Percent_Identity=47.3958333333333, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=47.9274611398964, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=43.979057591623, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=45.4005934718101, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=45.1038575667656, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=46.1794019933555, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI32565909, Length=183, Percent_Identity=32.7868852459016, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=45.0777202072539, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=44.8717948717949, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24580527, Length=390, Percent_Identity=45.8974358974359, Blast_Score=297, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24580525, Length=390, Percent_Identity=45.8974358974359, Blast_Score=297, Evalue=7e-81,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=45.6410256410256, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=45.6410256410256, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=45.6410256410256, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=45.6410256410256, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=45.6410256410256, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=45.6410256410256, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=43.8961038961039, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24580539, Length=162, Percent_Identity=38.2716049382716, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_GLUDA (A9HE98)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601515.1
- RefSeq:   YP_002276345.1
- ProteinModelPortal:   A9HE98
- SMR:   A9HE98
- GeneID:   5789179
- GeneID:   6975396
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1970
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42466; Mature: 42466

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKGDFLFTSESVSEGHPDKVADRISDTVLDAYLAADGESRVACETLVTTNRVVLAGEV
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHEECCEEEEEECC
RGPSSVTPEALIEGARAAIRDIGYDQAGFSWKNAQIESYLHAQSADIAVGVDSAGEKDEG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
AGDQGIMFGFATRETETLMPAPLFYAHGILHRIRDLRKAGDARVAMLQPDAKSQVTLRYV
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEE
DGRPVGATSVVISTQHDEGASQAAIREALRDVVQDVLPEGWMCPEDEFYANPTGVFVIGG
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLADRC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
TLQISYAIGVSHPLSVYVDLDGTGKDVDEAKLGSVLREVMNLTPRGIRQHLRLNRPIYTE
EEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEC
TSAYGHFGRTPDEARDNFTWEKTDLVDALRGAFNR
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNKGDFLFTSESVSEGHPDKVADRISDTVLDAYLAADGESRVACETLVTTNRVVLAGEV
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHEECCEEEEEECC
RGPSSVTPEALIEGARAAIRDIGYDQAGFSWKNAQIESYLHAQSADIAVGVDSAGEKDEG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
AGDQGIMFGFATRETETLMPAPLFYAHGILHRIRDLRKAGDARVAMLQPDAKSQVTLRYV
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEE
DGRPVGATSVVISTQHDEGASQAAIREALRDVVQDVLPEGWMCPEDEFYANPTGVFVIGG
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLADRC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
TLQISYAIGVSHPLSVYVDLDGTGKDVDEAKLGSVLREVMNLTPRGIRQHLRLNRPIYTE
EEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEC
TSAYGHFGRTPDEARDNFTWEKTDLVDALRGAFNR
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA